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<i>In Vitro</i>Regeneration of <i>Curcuma caesia</i>Plantlets from Basal Part and via Somatic Embryogenesis

Advances in Bioscience and Biotechnology 2014年第4期5卷 363-372页

作者： Ab. Rahman Zuraida Kamaruddin Fatin Liyana Izzati Othman Ayu Nazreena Che Mohd Zain Che Radziah sheikh ghadzi siti nur asyikin Subramaniam Sreeramanan Biotechnology Research Centre MARDI Headquarters Persiaran MARDI-UPM Serdang Malaysia Faculty of Science and Technology Universiti Kebang-saan Malaysia Bangi Malaysia School of Biological Sciences Universiti Sains Malaysia (USM) Minden Heights Malaysia

Plantlets of Curcuma caesia were produced in vitro from newly sprouting vegetative buds of tubers. Segments of the plantlets from the junction between the root and the basal portion of the stem were subsequently used as explants to investigate factors affecting callus induction and plant regeneration via somatic embryogenesis. The explants were placed on Woody Plant Medium (WPM) together with different concentrations of 2,4-dichlorophenoxyacetic acid(2,4-D) and benzyl aminopurine (BAP) in the presence of light. The growth medium supplemented with 5 mg/L BAP and 2 mg/L 2,4-D promoted callus induction after 70 days of culture. Sub-culturing on the same medium enhanced the production of friable callus. Culture media containing higher concentrations of agar promoted the development of green somatic embryos from the callus. Respond of somatic embryogenesis was most successful with MS medium in 6.0 g/L agar supplemented with 5 mg/L BAP and 0.2 mg/L 2,4-D whereby the callus developed into green somatic embryos with an efficiency of 53%. This culture medium also produced the largest number plantlets.

关键词： In Vitro Regeneration Somatic Embryogenesis Curcuma caesia

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Pharmacist intervention in home care program for diabetes patients

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Journal of Diabetes Mellitus 2012年第3期2卷 279-293页

作者： Syed Wasif Gillani Syed Azhar Syed Sulaiman Mirza Baig Yelly Oktavia Sari siti Maisharah sheikh ghadzi Sabariah Noor Haroon nur Hafzan Md Hanafiah Department of Clinical Pharmacy Aimst University Bedong Malaysia Faculty of Pharmacy Andalas University Padang Indonesia School of Pharmaceutical Sciences Universiti Sains Malaysia Kubang Kerian Malaysia School of Pharmaceutical Sciences Universiti Sains Malaysia Pulau Pinang Malaysia

Majority of research reports identified moderate reduction in glycated haemoglobin with education interventions regardless of age group. Our study objective was to evaluate the pharmacist interventions in providing patient home care. A 24-week longitudinal quasi-experimental—pre-test/post-test study design was used to assess the effectiveness of a diabetes education program to enhance self-care practices. A double-blinded randomized study design was considered but was not feasible as the investigator was responsible for implementing the intervention and collecting data on outcomes. Since this was a longitudinal study a 25% attrition rate was included in the calculation of sample size. Hence the sample size for the proposed study was 106 subjects with 53 subjects in each group. All analyses were done using SPSS version 18?. The level of significance was set at 0.05. The Research Ethics Committee of hospital and the Malaysian Medical Research and Ethics Committee approved the study. Of the 109 subject who met the study-entry criteria, 3 subjects declined to participate due to lack of time and interest. There was no significant relationship between the demographic and clinical characteristic of participants who completed the study. No significant relationship between the intervention and control groups who completed the study in demographic, clinical and psychosocial contexts. Of the 47 subjects from the intervention group who reported adherent to their daily medication intake after the education intervention, 51 subjects (31.9%) reported taking their medication at the wrong time. The recommended times for oral anti-hyperglycemic medication (OAM) are: sulphonylureas 30 minutes before food, acarbose with food, metformin with or within 30 minutes after food. This research has shown a brief structured education program that incorporated behavior science specifically self-efficacy was effective in enhancing self-care practices (SMBG and medication adherence) and improving

关键词： Diabetes Mellitus Interventional Study Longitudinal Study Pharmacist Services

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