检索结果-南通市图书馆

Functional expression of large-conductance calcium-activated potassium channels in human endometrium:an possible mechanism involved in endometrial receptivity and embryo implantation

在线全文

cnki会议

学校读者我要写书评

暂无评论

Functional expression of large-conductance calcium-activated...

2011中国妇产科学术会议暨浙江省计划生育与生殖医学学术年会暨生殖健康讲习班

作者： ZHANG Run-ju,ZOU Li-bo,WANG Ting-ting,LIU Ai-xia,QV Fan,DING Guo-lian,LU Yong-chao,sheng jian-zhong and huang he-feng department of reproductive endocrinology,Zhejiang Women’s Hospital,Zhejiang University School of Medicine, Hangzhou,Zhejiang,China department of Pathophysiology,Zhejiang University School of Medicine,Hangzhou,Zhejiang,China

Background Biomolecular mechanisms that are involved in the embryo implantation and development process are still *** studies showed that the functions of large-conductance calcium-activated potassium channels(BKca channels) were beyond traditional ion channel and they were the important integrators in many physiological systems. The purpose of the present study was to examine whether BK channels was expressed in uterine endometrium and its

关键词： Endometrium calcium-activated potassium channel,embryo implantation window of implantation

Adriamycin induces H2AX phosphorylation in human spermatozoa

在线全文

cnki会议

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2008年第5期10卷 749-757页

作者： zhong-Xiang Li Ting-Ting Wang Yan-Ting Wu Chen-Ming Xu Min-Yue Dong jian-zhong sheng he-feng huang department of reproductive endocrinology Women＇s Hospital Zhejiang University School of Medicine Hangzhou 310006 China department of Pharmacology and Therapeutics Faculty of Medicine University of Calgary Calgary Alberta T2N 4N1 Canada

Aim： To investigate whether adriamycin induces DNA damage and the formation of γH2AX （the phosphorylated form of histone H2AX） foci in mature spermatozoa. Methods： Human spermatozoa were treated with adriamycin at different concentrations, γH2AX was analyzed by immunofluorescent staining and flow cytometry and doublestrand breaks （DSB） were detected by the comet assay. Results： The neutral comet assay revealed that the treatment with adriamycin at 2 μg/mL for different times （0.5, 2, 8 and 24 h）, or for 8 h at different concentrations （0,4, 2 and 10 μg/mL）, induced significant DSB in spermatozoa. Immunofluorent staining and flow cytometry showed that the expression of γH2AX was increased in a dose-dependent and time-dependant manner after the treatment of adriamycin. Adriamycin also induced the concurrent appearance of DNA maintenance/repair proteins RAD50 and 53BP 1 with γH2AX in spermatozoa. Wortmannin, an inhibitor of the phosphatidylinositol 3-kinase （PI3K） family, abolished the co-appearance of these two proteins with γH2AX. Conclusion： Human mature spermatozoa have the same response to DSB-induced H2AX phosphorylation and subsequent recruitment of DNA maintenance/ repair proteins as somatic cells.

关键词： adriamycin human spermatozoa DNA double strand-breaks γH2AX

The effects of diabetes on male fertility and epigenetic regulation during spermatogenesis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2015年第6期17卷 948-953,I0008-I0009页

作者： Guo-Lian Ding Ye Liu Miao-E Liu Jie-Xue Pan Meng-Xi Guo jian-zhong sheng he-feng huang International Peace Maternity and Child health Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200030 China The Key Laboratory of reproductive Genetics Ministry of Education (Zhejiang University) Hangzhou 310058 China department of Pathology and Pathophysiology School of MedicineZhejiang University Hangzhou 310058 China

The effects of diabetes mellitus include long-term damages, dysfunctions, and failures of various organs. An important complication of diabetes is the disturbance in the male reproductive system. Glucose metabolism is an important event in spermatogenesis. Moreover, glucose metabolism is also important for maintaining basic cell activity, as well as specific functions, such as motility and fertilization ability in mature sperm. Diabetic disease and experimentally induced diabetes both demonstrated that either type 1 diabetes or type 2 diabetes could have detrimental effects on male fertility, especially on sperm quality, such as sperm motility, sperm DNA integrity, and ingredients of seminal plasma. Epigenetic modifications are essential during spermatogenesis. The epigenetic regulation represents chromatin modifications including DNA methylation, histone modifications, remodeling of nucleosomes and the higher-order chromatin reorganization and noncoding RNAs. If spermatogenesis is affected during the critical developmental window, embryonic gonadal development, and germline differentiation, environmentally-induced epigenetic modifications may become permanent in the germ line epigenome and have a potential impact on subsequent generations through epigenetic transgenerational inheritance. Diabetes may influence the epigenetic modification during sperm spermatogenesis and that these epigenetic dysregulation may be inherited through the male germ line and passed onto more than one generation, which in turn may increase the risk of diabetes in offspring.

关键词： diabetes epigenetic regulation sperm spermatogenesis

Long interpregnancy interval and adverse perinatal outcomes: a retrospective cohort study

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2020年第6期63卷 898-904页

作者： Jing Lin Han Liu Dan-Dan Wu Hong-Tao Hu Hui-Hui Wang Cheng-Liang Zhou Xin-Mei Liu Xiao-Jun Chen jian-zhong sheng he-feng huang International Peace Maternity and Child health Hospital School of MedicineShanghai Jiao Tong UniversityShanghai 200030China Institute of Embryo-Fetal Original Adult Disease Shanghai Key Laboratory for Reproductive MedicineSchool of MedicineShanghai Jiao Tong UniversityShanghai 200030China Shanghai Key Laboratory of Embryo Original Disease Shanghai 200030China department of Pathology and Pathophysiology School of MedicineZhejiang UniversityHangzhou 310058China

We conducted a retrospective cohort study of 9,552 women experiencing their second delivery between 2014 and 2016 at the International Peace Maternity and Child health Hospital to investigate the association between the interpregnancy interval(IPI)and adverse perinatal outcomes. With the 12–23-mon IPI as the reference category, logistic regression analyzes were used to examine associations between different IPIs(he 12–23-mon IPI category, women with longer IPIs had a higher risk of adverse perinatal outcomes, and those with an IPI ≥120 mon had the highest risk of gestational diabetes mellitus and premature membrane rupture(adjusted odds ratio(OR) 1.76, 95% confidence interval(CI) 1.32–2.35 and adjusted OR 2.03, 95% CI 1.53–2.67, respectively). These results indicate that a longer IPI is associated with a higher risk of adverse perinatal outcomes and an IPI of ≥120 mon appears to be independently associated with a higher risk of gestational diabetes mellitus and premature membrane rupture.

关键词： interpregnancy interval perinatal outcomes gestational diabetes mellitus premature membrane rupture

Pi3k/Pkc Pathway Mediates the Alteration of AQP7Expression and Location in Oocyte Induced by Cryoprotectants and Hyperosmolar Stimulation

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Pi3k/Pkc Pathway Mediates the Alteration of AQP7Expression a...

中国中西医结合学会生殖医学分会首届学术年会暨生殖医学专业委员会成立大会

作者： Ya-Jing Tan jian-zhong sheng he-feng huang department of reproductive Medicine International Peace Maternity and Child Health HospitalSchool of MedicineShanghai Jiao Tong University department of Pathology & Pathophysiology School of MedicineZhejiang University

Objective:To investigate molecular mechanisms of cryoprotectants and hyperosmolar stimulating oocytes AQP7 expression and localization ***:Detect the expression of AQP3,7 and 9 in human and mouse *** AQP3,7and 9 protein level after exposured in *** AQP7 protein location after exposured in hyperosmolar *** the signaling pathways of kinase activation and

关键词：

Basonuclin 1 deficiency causes testicular premature aging: BNC1 cooperates with TAF7L to regulate spermatogenesis

在线全文

cnki会议

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2020年第1期12卷 71-83页

作者： Jing-Yi Li Yi-feng Liu Hai-Yan Xu Jun-Yu Zhang Ping-Ping Lv Miao-E Liu Yan-Yun Ying Ye-Qing Qian Kun Li Cheng Li Yun huang Gu-feng Xu Guo-Lian Ding Yu-Chan Mao Chen-Ming Xu Xin-Mei Liu jian-zhong sheng Dan Zhang he-feng huang Key Laboratory of reproductive Genetics(Ministry of Education)and department of reproductive endocrinology Women's HospitalZhejiang University School of MedicineHangzhou 310006China International Peace Maternal and Child health Hospital Shanghai Jiao Tong UniversityShanghai 200030China department of Pathology&Pathophysiology Zhejiang University School of MedicineHangzhou 310000China

Basonuclin(BNC1)is expressed primarily in proliferative keratinocytes and gametogenic ***,its roles in spermatogenesis and testicular aging were not *** we discovered a heterozygous BNC1 truncation mutation in a premature ovarian insufficiency *** this study,we found that male mice carrying the truncation mutation exhibited progressively fertility loss and testicular premature ***-wide expression profiling and direct binding studies(by chromatin immunoprecipitation sequencing)with BNC1 in mouse testis identified several spermatogenesis-specific gene promoters targeted by BNC1 including kelch-like family member 10(Klhl1O),testis expressed 14(Tex14)9 and spermatogenesis and centriole associated 1(Spatcl).Moreover,biochemical analysis showed that BNC1 was associated with TATA-box binding protein-associated factor 7 like(TAF7L),a germ cell-specific paralogue of the transcription factor IID subunit TAF7,both in vitro and in testis,suggesting that BNC1 might directly cooperate with TAF7L to regulate *** truncation mutation disabled nuclear translocation of the BNC1/TAF7L complex,thus,disturbing expression of related genes and leading to testicular premature ***,expressions of Y-box-binding protein 2(YBX2),outer dense fiber of sperm tails 1(ODfl),and glyceraldehyde-3-phosphate dehydrogenase,spermatogenic(GAPDHS)were significantly decreased in the testis of men with non-obstructive *** present study adds to the understanding of the physiology of male reproductive aging and the mechanism of spermatogenic failure in infertile men.

关键词： testicular aging spermatogenesis BNC1 TAF7L gene mutation

The Role of Large-C onductance Calcium-A ctivated Potassium Channels in Blastocyst Implantation

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Role of Large-C onductance Calcium-A ctivated Potassium ...

2009中国杭州生殖健康学术论坛暨浙江省计划生育学与生殖医学分会学术年会

作者： department of endocrinology Women’s Hospital School of Medicine Zhejiang University China Run Ju Zhang Ping Ping Lv he feng huang department of pathophysiology School of Medicine Zhejiang University China jian zhong sheng

Background Endometrium receptivity is essential for the success of embryonic implantation into endometrial *** fundamental moderators to endometrium receptivity are 17-beta oestradiol and *** the fact that BK has been found expressed on the human endometrium,this study is to investigate the role played by BK in the pathway through which endometrium receptivity is regulated by 17β-estradiol and progestone.

关键词：

Metabolic Risk Associated with Intrauterine Environment of Gestational Diabetes Mellitus

在线全文

cnki会议

学校读者我要写书评

暂无评论

Metabolic Risk Associated with Intrauterine Environment of G...

2009中国杭州生殖健康学术论坛暨浙江省计划生育学与生殖医学分会学术年会

作者： department of endocrinology Women’s Hospital School of Medicine Zhejiang University China Guo Lian Ding Fang Fang Wang he feng huang department of pathophysiology School of Medicine Zhejiang University China jian zhong sheng

Background Since hyperglycemia is a major characteristic of gestational diabetes mellitus(GDM),the importance of the intrauterine environment is highlighted by studies in the general population that indicate an association between poor fetal growth followed by subsequent risk of diabetes in ***,the mechanism of intrauterine hyperglycemia associated diabetes in offspring is still unclear.

关键词：

FGF2 bind to FGFR2 in trophectoderm cells is indispensable for expanded blastocyst formation and is activated via PKC-p38 signalling pathway

在线全文

cnki会议

学校读者我要写书评

暂无评论

FGF2 bind to FGFR2 in trophectoderm cells is indispensable f...

中国中西医结合学会生殖医学分会首届学术年会暨生殖医学专业委员会成立大会

作者： Jing Yang Yong-Chao Lu Xian-Hua Lin Ying Yu Ying-Hui Ye Liang Jin Dan Zhang jian-zhong sheng he-feng huang International Peace Maternity and Child health Hospital Shanghai Jiao Tong University School of Medicine The Key Laboratory of reproductive Genetics Ministry of Education(Zhejiang University) department of Pathology and Pathophysiology School of MedicineZhejiang University

BACKGROUND:The fibroblast growth factors(FGFs) binding to fibroblast growth factor receptors(FGFRs) plays important roles in the formation of the inner cell mass(ICM) lineages in mouse embryo ***,its role in trophectoderm(TE) remains ***:In the present study,we investigated which and how FGFs/FGFRs in trophectoderm cells(TEs) acted in expanded blastocyst formation using embryo culture,quantitative real-time PCR,immunofluorescence labeling,confocal microscopy,embryo culture,siRNA transfection and ***:The FGF2/FGFR2 in TEs was required for expanded blastocyst ***2 or FGFR2 knockdown in blastocyst suppressed the expression and altered the distribution of E-cadherin,ZO-1,Na-K ATPase,epithelial Na channel(ENaC),aquaporin(AQP) 3 and AQP 9 in *** with protein kinase C(PKC) inhibitor,G66983,suppressed expanded blastocyst formation,p-p38 translocation and the above proteins ***,p38 MAPK inhibitor,SB202190,also blocked p-p38 translocation and the above proteins ***:The present results suggest that FGF2/FGFR2 may act in an autocrine fashion and activate downstream PKC/p38 pathway in TEs in expanded blastocyst formation.

关键词： fibroblast growth factor 2 fibroblast growth factor receptor 2 expanded blastocyst formation protein kinase C p38 MAPK