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Chinese Chemical Letters 2010年第11期21卷 1361-1365页

作者： Jian she tang Li Xiang National Experimental Teaching Demonstration Laboratory of Water Pollution Control and Recovery Utilization from Wastewater Anhui University of Architecture Hefei 230022 China

A rapid,simple,and reliable competitive molecular imprinted polymer sorbent assay（MIPSA） was developed and validated for measurement of parathion in water *** assay employed a molecular imprinted polymer（MIP） that was synthesized with non-covalent imprinting method as capture reagent and p-aminoparathion conjugate of horseradish peroxidase（para-HRP） as an enzyme *** assay depended on a competitive binding reaction between the enzyme conjugate and analyte for the binding sites of the *** optimized analysis conditions of 10 ng mL-1 para-HRP and 10 mg mL-1 polymer were *** assay was acceptable to detect parathion in water samples under the optimized conditions,with a limit of detection of 50 ng *** analytical recoveries of added parathion in water samples ranged from 101.2%to 105%.The precision of the assay was satisfactory; relative standard deviation ranged from 4.3%to 6%.

关键词： Molecular imprinting Conjugated enzyme Sorbent assay Parathion Water sample

Effects of soilless substrates on seedling quality and the growth of transplanted super japonica rice

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Journal of Integrative Agriculture 2017年第5期16卷 1053-1063页

作者： LEI Wu-sheng DING Yan-feng LI Gang-hua tang she WANG Shao-hua Jiangsu Collaborative Innovation Center for Modern Crop Production/National Engineering and Technology Center for Information Agricultrue/Key Laboratory of Crop Physiology and Ecology in Southern China Nanjing Agricultural UniversityNanjing 210095P.R.China Jiangsu Vocational College of Agriculture and Forestry Jurong 212400P.R.China

This study was conducted to investigate the effects of soilless substrates of hydroponically grown long-mat seedlings （HLMS） on seedling quality and field growth characteristics of transplanted super japonica rice. A widely grown conventional super japonica rice cultivar （Wuyunjing 23） was selected as the test material. The effect of HLMS on seedling quality, mechanical transplantation quality, field growth characteristics, yield, and benefit-cost ratio were compared with seedlings grown in organic substrates and traditional nutritive soil, whJch was selected as the control. Root number, root twJstJng power and root activity of seedlings cultivated by HLMS were decreased compared to that of the organic substrates andcontrol. However, seedling root length as well as aboveground growth were increased compared to the organic substrates and control seed- lings. In the HLMS, the content of gibberellin acid （GA3） decreased while abscisic acid （ABA） content increased compared to that of the organic substrates and control seedlings. During the early stages after transplanting, the re-greening of HLMS was delayed compared to that of the organic substrates and control seedlings. Nevertheless, there were no significant differences in tiller dynamics and crop yield among the HLMS, organic substrates and control treatments. The effects of HLMS on seedling production were similar to those of the organic substrates and traditional nutritive soil in the present study, suggesting that HLMS have the potential to replace traditional nutritive soil in seedling production without decreasing crop yield. Finally, it is important to reduce organic substrates and topsoil dependence during rice seedling production and worthwhile to consider HLMS popularization and its application on a larger scale.

关键词： super hybrid rice (Oryza sativa L.) mechanical transplantation seedling quality yield seedling raised method

Effects of rice or wheat residue retention on the quality of milled japonica rice in a rice–wheat rotation system in China

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The Crop Journal 2015年第1期3卷 67-73页

作者： Pengfu Hou Yanfeng Ding Guofa Zhang Quan Li Shaohua Wang she tang Zhenghui Liu Chengqiang Ding Ganghua Li College of Agronomy Nanjing Agricultural University/Key Laboratory of Crop Physiology Ecology and Production ManagementMinistry of Agriculture Daqing Normal University

In rice–wheat rotation systems, crop straw is usually retained in the field at land preparation in every, or every other, season. We conducted a 3-year-6-season experiment in the middle–lower Yangtze River Valley to compare the grain qualities of rice under straw retained after single or double seasons per year. Four treatments were designed as: both wheat and rice straw retained(WR), only rice straw retained(R), only wheat straw retained(W), and no straw retained(CK). The varieties were Yangmai 16 wheat and Wuyunjing 23 japonica rice. The results showed contrasting effects of W and R on rice quality. Amylopectin content, peak viscosity, cool viscosity, and breakdown viscosity of rice grain were significantly increased in W compared to the CK, whereas gelatinization temperature,setback viscosity, and protein content significantly decreased. In addition, the effect of WR on rice grain quality was similar to that of W, although soil fertility was enhanced in WR due to straw being retained in two cycles. The differences in protein and starch contents among the treatments might result from soil nitrogen supply. These results indicate that wheat straw retained in the field is more important for high rice quality than rice straw return, and straw from both seasons is recommended for positive effects on soil fertility.

关键词： Japonica rice Crop straw Grain quality Paddy–upland rotation

Effects of culm carbohydrate partitioning on basal stem strength in a high-yielding rice population

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The Crop Journal 2017年第6期5卷 478-487页

作者： Jun Zhang Ganghua Li Qingyu Huang Zhenghui Liu Chengqiang Ding she tang Lin Chen Shaohua Wang Yanfeng Ding Weijian Zhang Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing 100081 China Jiangsu Key Laboratory for Information Agriculture/National Engineering and Technology Center for Information/Agriculture Key Laboratory of Crop Physiology and Ecology in Southern China Nanjing Agricultural University Nanjing 210095 Jiangsu China Agricultural Technology Promotion Station of Taoyuan Township in Yongsheng County Yongsheng 674205 Yunnan China

Rice culm carbohydrate transport can simultaneously affect grain filling and stem lodging resistance by regulating non-structural carbohydrate(NSC) and structural carbohydrate(SC) contents. However, the relationship between carbohydrate transposition and culm strength is not well documented. Accordingly, a high-yielding hybrid rice cultivar(Y Liangyou 2) was tested under different N fertilization regimes at two locations, Taoyuan(a special high-yield eco-site), Yunnan province and Danyang(a representative eco-site of the middle and lower Yangtze), Jiangsu province, China. Significantly higher grain yield and basal stem strength were found at Taoyuan than Danyang under all N rates throughout the two-year experiment. At heading stage, soluble sugars, starch, cellulose and lignin contents of the basal culm at Taoyuan were significantly 132.0%, 73.7%, 1.2%, and 62.7% higher than those at Danyang, respectively. At 20 days after heading, soluble sugars and starch content at Taoyuan decreased significantly compared to Danyang, but lignin content remained higher. Culm carbohydrate transport to kernels at Taoyuan was significantly greater than that at Danyang, and the proportion of soluble sugars and starch was correspondingly 62.9%lower. However, the proportion of lignin and cellulose was 22.7% higher at Taoyuan than that at Danyang. Soluble sugars and starch partitioning were significantly reduced under an increased nitrogen application rate, but SC partitioning was little affected. There were significant positive correlations between basal culm bending stress and dry weight and cellulose and lignin proportions at both locations under all N rates, suggesting that the higher SC proportion at 20 days after heading was primarily responsible for culm *** results suggest that high-yielding rice populations with greater culm strength require both moderate NSC transport and greater SC accumulation.

关键词： Rice High-yielding Lodging Culm strength Carbohydrate partitioning

Charactering protein fraction concentrations as influenced by nitrogen application in low-glutelin rice cultivars

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Journal of Integrative Agriculture 2016年第3期15卷 537-544页

作者： LI Gang-hua CHEN Yi-lu DING Yan-feng GENG Chun-miao LI Quan LIU Zheng-hui WANG Shao-hua tang she Jiangsu Collaborative Innovation Center for Modern Crop Production/Jiangsu Key Laboratory for Information Agriculture/National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China Nanjing Agricultural University Nanjing 210095 P.R.China

To optimize both grain yield and quality of low-glutelin rice cultivars under N-fertilizer strategies, two-year field experiments involving three low-glutelin rice cultivars（W1240, W1721, W025） and an ordinary rice cultivar（H9405） with five N treatments were carried out to determine the effects of N application rate and genotype on protein fractions contents and Glutelin/Prolamin ratio（Glu/Pro）. The difference of protein fraction concentrations affected by N application rate existed in genotypes. Ordinary rice cultivar had a larger increase in glutlein concentration affected by N application rate than low-glutelin rice cultivars did. Glutelin in H9405 had a increase of 30.6 and 41.0% under the N4 treatment（360 kg N ha^（–1）） when compared with N0 treatment（no fertilizer N） in 2010 and 2011 respectively, while all the low-glutelin rice cultivars showed relatively smaller increases for two years. Variance analysis showed no significant effect of N application rate on glutelin in W1240 and W025 while the effects on albumin, globulin and prolamin were significant in low-glutelin rice. What＇s more, N treatment had no significant i nfluence on Glu/Pro ratios in low-glutelin rice cultivars while a significant increase in Glu/Pro ratio was observed in ordinary rice cultivar. So low-gultelin rice cultivars showed a different pattern from ordinary rice cultivars when influenced by N application rate.

关键词： low-glutelin rice nitrogen protein fractions Glu/Pro

Variation in Yield and Physicochemical Quality Traits among Mutants of Japonica Rice Cultivar Wuyujing 3

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Rice science 2016年第1期23卷 33-41页

作者： Jose Daniel ABACAR LIN Zhao-miao ZHANG Xin-cheng DING Cheng-qiang tang she LIU Zheng-hui WANG Shao-hua DING Yan-feng College of Agronomy Nanjing Agricultural University Nanjing 210095 China Jiangsu Collaborative Innovation Center for Modern Crop Production Nanjing 210095 China

To select elite germplasms, 112 mutants derived from japonica rice cultivar Wuyujing 3 were evaluated. The yield components such as panicle number per square meter, grain number per panicle, and grain weight were measured. The quality traits such as percentage of chalky grains（PCG）, brown rice yield（BRY）, milled rice yield（MRY）, degree of milling（DM）, amylose content（AC）, protein content（PC）, and relationships among traits were inverstigated. Results showed that grain yield ranged from 2.15 to 12.49 t/hm2 with a mean of 6.4 t/hm2 and number of grains per square meter contributed for 94.64% in grain yield variation. For quality traits, all rice mutants had short size（grain length ≤ 5.5 mm） and bold shape（grain length to width ratio = 1.10-2.00）. Most of rice mutants（87.5%） had PCG values below 20%. All mutants had MRY values above 50%, AC values below 20%, and PC values below 10%. Percentage of chalky grains was significantly negatively correlated with MRY and positively correlated with DM. BRY and MRY were significantly negatively correlated with DM. PC was significantly and positively correlated with MRY and negatively correlated with DM, while AC had no significant correlation with these quality traits. It was concluded that there were 25 rice mutants which fulfilled the major requirements of Jiangsu standard japonica rice such as low percentage of chalky grains, low amylose content, optimal protein content, and which could be used as elite germplasms. Thus the mutants identified may lead to significant progress in improvement of rice quality.

关键词： japonica rice mutant yield appearance quality milling quality nutritional quality

Effects of Nitrogen Application Rate and Ratio on Lodging Resistance of Super Rice with Different Genotypes

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Journal of Integrative Agriculture 2014年第1期13卷 63-72页

作者： ZHANG Wu-jun LI Gang-hua YANG Yi-ming LI Quan ZHANG Jun LIU Jin-you WANG Shao-hua tang she DING Yan-feng National Engineering and Technology Center for Information Agriculture Ministry of Industry and Information Technology/Jiangsu Key Laboratory for Information Agriculture Science and Technology Department of Jiangsu Province/College of Agriculture Nanjing Agricultural University Nanjing 210095 P.R. China Agronomy College Nanjing Agricultural University/Key Laboratory of Crop Physiology & Ecology in Southern China Ministry of Agriculture Nanjing 210095 P.R. China

The objective of this study was to determine the morphology mechanism of nitrogen （N） fertilizer rates and ratio on lodging resistance through analying its effects among lodging index （LI）, lodging-related morphological traits and physical strength in basal intemodes by comparing japonica and indica super rice cultivars. Field experiments, with three nitrogen levels （0, 150 and 300 kg ha-L） and two ratios of basal to topdressing （8：2 and 5：5） with two super rice cultivars （Yliangyou 2 and Wuyunjing 23）, were conducted in the Baolin Farm, Danyang Country, Jiangsu Province, China, in 2011 and 2012. Effects of N fertilizer rates and ratios on morphology of whole plant, morphology traits in basal intemodes and culm＇s physical strength parameters were investigated at 20 d after full heading stage. LI of Yliangyou 2 was significant greater than that of Wuyunjing 23 due to larger bending moment by whole plant （WP） with higher plant height and gravity center height. With higher volume of N fertilizer, LI of two super rice cultivars was increased conspicuously. However, no significant effect was detected with increase of panicle fertilizer ratio. The size of breaking strength （M） in basal intemodes was the key factor determining LI among N fertilizer treatments. Correlation analysis revealed that M value was positively related bending stress （BS） of Wuyunjing 23 and section modulus （Z） of Yliangyou 2, respectively. The higher N fertilizer levels induced reduction ofBS of Wuyunjing 23 due to weak culm and leaf sheath plumpness status and reduced Z of Yliangyou 2 owning to small diameter and culm wall thickness, consequently, influencing their M indirectly. These results suggested that breaking strength was the key factor influencing LI with increase of N fertilizer levels. However, the lodging-related morphology mechanism was different with genotypes. Culm wall thickness and diameter in basal internodes of indica super rice and culm and leaf sheath plumpness status of japonic

关键词： super rice nitrogen fertilizer application management lodging resistance culmmorphology

Light-responsive nanomaterials for biofilm removal in root canal treatment

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Chinese Chemical Letters 2025年第2期36卷 38-48页

作者： Di An Mingdong she Ziyang Zhang Ting Zhang Miaomiao Xu Jinjun Shao Qian shen Xuna tang Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM) Nanjing Tech University Department of Senior Expert Diagnosis and Treatment Center Nanjing Stomatological Hospital Affiliated Hospital of Medical School Nanjing University State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors Institute of Advanced Materials (IAM)Nanjing University of Posts and Telecommunications

Various chemical irrigants and drugs have been employed for intra-canal disinfection in root canal therapy (RCT). However, due to the complexity of root canal anatomy, many drugs still exhibit poor penetrability and antibiotic resistance, leading to suboptimal treatment outcomes. Thus, it is challenging to remove the organic biofilms from root canals. In recent years, light-responsive therapy, with deeper tissue penetration than traditional treatments, has emerged as an effective RCT modality. Herein, this review summarizes the recent development of light-responsive nanomaterials for biofilm removal in *** light-responsive nanomaterials and the corresponding therapeutic methods in RCT, including photodynamic therapy (PDT), photothermal therapy (PTT), and laser-activated therapy, are highlighted. Finally,the challenges that light-responsive nanomaterials and treatment modalities will encounter to conquer the biofilm in future RCT are discussed. This review is believed to significantly accelerate the future development of light-responsive nanomaterials for RCT from bench to bedside.

关键词： Biofilm Light-responsive nanomaterials Photodynamic therapy Photothermal therapy Root canal treatment

Top-dressing nitrogen fertilizer rate contributes to decrease culm physical strength by reducing structural carbohydrate content in japonica rice

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Journal of Integrative Agriculture 2016年第5期15卷 992-1004页

作者： ZHANG Wu-jun WU Long-mei DING Yan-feng WENG Fei WU Xiao-ran LI Gang-hua LIU Zheng-hui tang she DING Cheng-qiang WANG Shao-hua Jiangsu Collaborative Innovation Center for Modern Crop Production/Jiangsu Key Laboratory for Information Agriculture/National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China Nanjing Agricultural University Nanjing 210095 P.R. China

Lodging is an important factor limiting rice yield and quality by bending or breaking stem in japonica rice（Oryza sativa L.） production. The objectives of this study were to determine the mechanism of lodging resistance in japonica rice as affected by carbohydrate components, especially its related arrangement in culm tissue and response to top-dressing nitrogen（N） fertilizer. Field experiments were conducted in Danyang County, Jiangsu Province, China, by using two japonica rice varieties Wuyunjing 23（lodging-resistance variety） and W3668（lodging-susceptible variety） with three top-dressing N fertilizer rates（0, 135 and 270 kg N ha^（-1）） in 2013 and 2014. Lodging related physical parameters, morphological characteristics and stem carbohydrate components were investigated at 30 d after full heading stage. Results showed that with increasing N fertilizer rates, the lodging rate and lodging index increased rapidly primarily due to significant reduction of breaking strength in two japonica rice varieties. Correlation analysis revealed that breaking strength was significantly and positively correlated with bending stress, but negatively correlated with section modulus, except for significant correlation at W3668 in 2014. Higher stem plumpness status and structural carbohydrate contents significantly enhanced stem stiffness, despite of lower non-structural carbohydrate. With higher N fertilizer rate, the culm wall thickness was almost identical, and culm diameter increased slightly. The structural carbohydrates, especially for lignin content in culm, reduced significantly under high N rate. Further histochemical staining analysis revealed that high N treatments decreased the lignin deposition rapidly in the sclerenchyma cells of mechanical tissue, large vascular bundle and small vascular bundle region, which were consistent with reduction of bending stress, especially for W3668 and thus, resulted in poor stem strength and higher lodging index. These results suggested that

关键词： japonica rice lodging resistance nitrogen stem strength structural carbohydrate

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浅议媒体整合

西南民族大学学报(人文社会科学版) 2001年第2期22卷 135-136页

作者：唐陟四川大学新闻系!2000级硕士研究生

随着市场竞争的加剧,各种传播媒体都希望自身变得更加强大,以便在激烈的竞争中处于有利地位.于是,媒体合作就成为媒体竞争加剧过程中涌现的时代新潮.应当怎样把握这种媒体运作的新态势,是值得理论界和实务界共同关注的前沿课题.这里发... 详细信息

随着市场竞争的加剧,各种传播媒体都希望自身变得更加强大,以便在激烈的竞争中处于有利地位.于是,媒体合作就成为媒体竞争加剧过程中涌现的时代新潮.应当怎样把握这种媒体运作的新态势,是值得理论界和实务界共同关注的前沿课题.这里发表的一组笔谈,对这一课题从不同的角度进行了多方面探讨,提出了各自的见解,有助于我们认识媒体既竞争又合作的这一运作新动向.

关键词：媒体整合传统媒体报纸网站报刊行政性垄断浅议