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Endo-β-1,4-glucanases impact plant cell wall development by influencing cellulose crystallization

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Journal of Integrative plant Biology 2015年第4期57卷 396-410页

作者： Magdalena Glass Sarah Barkwill Faride Unda shawn D.Mansfield Department of Wood Science University of British Columbia

Cell walls are vital to the normal growth and development of plants as they protect the protoplast and provide rigidity to the stem. Here, two poplar and Arabidopsis orthologous endoglucanases, which have been proposed to play a role in secondary cell wall development, were examined. The class B endoglucanases, pt GH9B5 and At GH9B5, are secreted enzymes that have a predicted glycosylphosphatidylinositol anchor, while the class C endoglucanases, pt GH9C2 and At GH9C2, are also predicted to be secreted but instead contain a carbohydrate-binding *** poplar endoglucanases were expressed in Arabidopsis using both a 35 S promoter and the Arabidopsis secondary cell wall-specific Ces A8 promoter. Additionally, Arabidopsis t-DNA insertion lines and an RNAiconstruct was created to downregulate At GH9C2 in Arabidopsis. All of the plant lines were examined for changes in cell morphology and patterning, growth and development, cell wall crystallinity, micro fibril angle, and proportion of cell wall carbohydrates. Misregulation of pt GH9B5/At GH9B5 resulted in changes in xylose content, while misregulation of pt GH9C2/At GH9C2 resulted in changes in crystallinity, which was inversely correlated with changes in plant height and rosette diameter. Together, these results suggest that these endoglucanases affect secondary cell wall development by contributing to the cell wall crystallization process.

关键词： Cellulose cell walls crystallinity endoglucanase expansion glucose xylose

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A 3D-printed modular magnetic digital microfluidic architecture for on-demand bioanalysis

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Microsystems & Nanoengineering 2020年第1期6卷 785-795页

作者： pojchanun Kanitthamniyom Aiwu Zhou Shilun Feng Aiqun Liu shawn Vasoo Yi Zhang Singapore Centre for 3D printing School of Mechanical and Aerospace EngineeringNanyang Technological UniversitySingaporeSingapore School of Mechanical and Aerospace Engineering Nanyang Technological UniversitySingaporeSingapore School of Electrical and Electronic Engineering Nanyang Technological UniversitySingaporeSingapore National Center for Infectious Disease Tan Tock Seng HospitalSingaporeSingapore

Magnetic digital microfluidics(MDM)manipulates fluids in the form of droplets on an open substrate,and incorporates surface energy traps(SETs)to facilitate the droplet *** MDM devices are fabricated monolithically,which makes it difficult to modify the device configuration without completely overhauling the original *** this paper,we present a modular MDM architecture that enables rapid on-demand configuration and re-configuration of MDM platforms for customized *** modular component contains a SET and a Lego-like antistud that fits onto a base board with Lego-like *** illustrate the versatility of the modular MDM architecture in biomarker sensing,pathogen identification,antibiotic resistance determination,and biochemical quantification by demonstrating immunoassays,phenotypical assays and enzymatic assays on various modular MDM platforms configured on demand to accomplish the fluidic operations required by assorted bioanalytical *** modular MDM architecture promises great potential for point-of-care diagnostics by offering on-demand customization of testing platforms for various categories of diagnostic *** also provides a new avenue for microfluidic assay development with its high configurability which would significantly reduce the time and cost of the development cycle.

关键词： configuration operations enable

Implementation science for the adductor canal block:A new and adaptable methodology process

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World Journal of Orthopedics 2021年第11期12卷 899-908页

作者： Nikhil Crain Chun-Yuan Qiu Stephen Moy shawn Thomas Vu Thuy Nguyen Mijin Lee-brown Diana Laplace Jennifer Naughton John Morkos Vimal Desai Bowman Gray Center for Medical Education Wake Forest School of MedicineWinston-SalemNC 27103United States perioperative Service and Anesthesiology Kaiser Permanente Medical CenterBaldwin ParkCA 91706United States Department of Anesthesiology Baldwin Park Medical CenterSouthern California Kaiser Permanente Medical GroupBaldwin ParkCA 91706United States Johns Hopkins University The Johns Hopkins University School of MedicineBaltimoreMD 21205United States

BACKGROUND Following the successful perioperative Surgical Home(pSH)practice for total knee arthroplasty(TKA)at our institution,the need for continuous improvement was realized,including the deimplementation of antiquated pSH elements and introduction of new *** To investigate the transition from femoral nerve blocks(FNB)to adductor canal nerve blocks(ACB)during *** Our 13-month study from June 2016 to 2017 was divided into four periods:a three-month baseline(103 patients),a one-month pilot(47 patients),a three-month implementation and hardwiring period(100 patients),and a six-month evaluation period(185 patients).In total,435 subjects were *** within 30 postoperative days were extracted from electronic medical records,such as physical therapy results and administration of oral morphine equivalents(OME).RESULTS Our institution reduced FNB application(64% to 3%)and increased ACB utilization(36% to 97%)at 10 *** in the ACB group were found to have increased ambulation on the day of surgery(4.1 vs 2.0 m)and lower incidence of falls(0 vs 1%)and buckling(5% vs 27%)compared with FNB patients(ppatients(13.9)reported lower OME than FNB patients(15.9),the difference(p=0.087)did not fall below our designated statistical threshold of p valuep”within 6 mo,our institution’s findings demonstrate evidence in the value of implementation *** education,technical support,and performance monitoring were deemed key facilitators of our program’s *** patient populations and additional orthopedic procedures are recommended for future study.

关键词： Total knee arthroplasty Femoral nerve block Adductor canal block physical therapy Oral morphine equivalent Action-related information gap

Acoustic Scene Classification via Classifiers Voting

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Acoustic Scene Classification via Classifiers Voting

作者： shawn Sudheer Sagar 华南理工大学

学位级别：硕士

Acoustic Scene Classification（ASC）is the recognition and categorization of audio data that identifies the environment which it has been *** is quite a challenging application of machine listening due to the noisy nature of the audio *** analyze several state-of-the-art models for ASC on two different *** datasets belong to the IEEE challenge on the Detection and Classification of Acoustic Scenes and Events（DCASE）2017 and *** two datasets are publicly available for research purpose,and consist of 10 and 15 classes of acoustic scenes *** total,57 hours of stereo recordings are available which includes common indoor and outdoor environmental scenes,such as beach,city center,library,forest path train,car,*** propose a method of ASC via fusing the voting of deep neural *** the proposed method,two different acoustic features are first extracted from each audio recording *** cepstral coefficients（MFCC）and Logarithmic filter-bank（LFB）.These features are then fed into three different classifiers（deep neural networks）: Visual Geometry Group（VGG）,Residual Network（Res Net）and Long Short-Term Memory（LSTM）.The motivation for choosing these variety of neural networks is that they have complementary advantages for *** training each network and acquiring the results,fusion of classifiers voting is used to determine a final *** fusion of all the results through voting technique is one of the methods under ensemble *** final Classification Accuracies（CA）that are obtained after the fusion of the classifiers are 73.27% and 76.99% on DCASE 2017 and 2019 datasets *** proposed fusion of classifiers voting obtains the CA improvements by 12.71% and 13.79% on DCASE 2017 and 2019 datasets respectively,compared to the individual baseline classifiers.

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Using Folding Ensemble and Stem probability Maximization Methods to predict RNA H-Type pseudoknots

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Tsinghua Science and Technology 2012年第6期17卷 691-700页

作者： Junilda Spirollari shawn Xiong Wang Jason T.L. Wang Bioinformatics program Department of Computer Science New Jersey Institute of Technology University Heights Department of Computer Science California State University

We present in this paper an ab initio method, named KnotFold, for RNA H-type pseudoknot prediction. Our method employs an ensemble of RNA folding tools and a filtering heuristic to generate a set of pseudoknot-free stems, and then predicts pseudoknots by utilizing a search technique with a pseudo-probability scoring scheme. Experimental results show that KnotFold achieves higher sensitivity than existing methods. The KnotFold package with documentation is freely available at http：//***/KnotFold.

关键词： RNA structure pseudoknots tool ensemble

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Optimal Retrofit Scheme for Highway Network under Seismic Hazards

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International Journal of Transportation Science and Technology 2014年第2期3卷 109-128页

作者： Yongxi Huang shawn parmelee Weichiang pang Glenn Department of Civil Engineering Clemson UniversityClemsonSC 29634 Civil Engineer AMEC400 Executive Center Dr.GreenvilleSC 29615 Assistant professor 1-864-656-0114lenn Department of Civil EngineeringClemson UniversityClemsonSC 29634

Many older highway bridges in the United States(US)are inadequate for seismic loads and could be severely damaged or collapsed in a relatively small *** to the most recentAmerican Society of Civil Engineers’infrastructure report card,one-third of the bridges in the US are rated as structurally deficient and many of these structurally deficient bridges are located in seismic *** improve this situation,at-risk bridges must be identified and evaluated and effective retrofitting programs should be in place to reduce their seismic *** this study,a new retrofit strategy decision scheme for highway bridges under seismic hazards is developed and seamlessly integrate the scenario-based seismic analysis of bridges and the traffic network into the proposed optimization modeling framework.A full spectrum of bridge retrofit strategies is considered based on explicit structural assessment for each seismic damage *** an empirical case study,the proposed retrofit strategy decision scheme is utilized to evaluate the bridge network in one of the active seismic zones in the US,Charleston,South *** developed modeling framework,on average,will help increase network throughput traffic capacity by 45%with a cost increase of only$15million for the Mw 5.5 event and increase the capacity fourfold with a cost of only$32m for the Mw 7.0 event.

关键词： Retrofit Seismic hazards Optimization Highway network

The use of woods-run chips in filter socks to control erosion and sedimentation during petroleum development in the Appalachian Basin

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International Journal of Sediment Research 2019年第6期34卷 608-615页

作者： shawn T.Grushecky Louis M.McDonald Lawrence Osborn West Virginia University Davis College of Agriculture Natural Resources and Design School of Natural Resources Appalachian Hardwood Center at West Virginia University School of Natural Resources Energy Land Management 3. Division of Plant and Soil SciencesEnvironmental Soil Chemistry and Soil Fertility

Filter socks frequently are used for erosion and sediment perimeter control during oil and gas development activities in the Appalachian Basin of the United *** specify the use of composted wood material for sock *** specification,as opposed to non-composted or fresh wood chips(woods-run),has created inefficiencies during well site *** than use fresh wood chips created during site construction,composted chips must be procured and used as filter sock media for erosion and sedimentation *** woods-run chips could be used as filter sock media instead of composted chips,there could be a significant reduction in energy/capital costs,truck traffic,and disposal *** primary objective of this research project was to compare the effectiveness of woodsrun material versus traditionally composted wood chips in controlling sediment transport as well as other chemical and physical parameters in a laboratory *** significant differences in pH(5.96 versus 6.02) or conductivity(0.029 dS/m versus 0.035 dS/m) were found in sediment laden water filtered through woods-run versus composted chips,***,chip particle sizes were outside the allowable limits for composted sock media,and moisture content also was outside the specified limits for woods-run ***(NO) concentrations were significantly higher in woods-run,while phosphorus(p) and potassium(K) concentrations were greater in composted chips;however none of the N,p,or K concentrations were above the regulatory ***,no difference in the filtering efficiency or time was found between woods-run and composted *** laboratory results indicate that current regulations allowing the use of woods-run chips in all but the highest quality watersheds is justified.

关键词： BMp Filter socks petroleum exploration Marcellus shale Wood chips

Lithium-ion battery data and where to find it

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Energy and AI 2021年第3期5卷 255-269页

作者： Gonçalo dos Reis Calum Strange Mohit Yadav shawn Li School of Mathematics University of EdinburghThe King’s BuildingsEdinburgh EH93FDUK Centro de Matemática e Aplicações(CMA) FCTUNLQuinta da Torre2829-516 CaparicaPortugal Indian Institute of Technology KanpurIndia

Lithium-ion batteries are fuelling the advancing renewable-energy based *** the core of transformational developments in battery design,modelling and management is *** this work,the datasets associated with lithium batteries in the public domain are *** review the data by mode of experimental testing,giving particular attention to test variables and data *** highlighted tools and platforms,over 30 datasets are reviewed.

关键词： Lithium battery public data Battery data Battery tests Machine learning

Using microglia-derived extracellular vesicles to capture diversity of microglial activation phenotypes following neurological injury

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Neural Regeneration Research 2024年第8期19卷 1633-1634页

作者： Austyn D.Roseborough Nikita Ollen-Bittle shawn NWhitehead Vulnerable Brain Laboratory Department of Anatomy&Cell BiologySchulich School of Medicine&DentistryLondonOHCanada

Microglia are one of the three glial cell populations in the central nervous system(CNS),along with astrocytes and *** microglia are unique among brain cells due to their hematologic origin and perform immune function... 详细信息

关键词： neurological cytes activation

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