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Results from the national China hospital invasive fungal surveillance net(CHIF-NET ) study,2011:yeast Species and in vitro susceptibilities to fluconazole and voriconazole

Results from the national China hospital invasive fungal sur...

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2012全国临床微生物与感染免疫学术研讨会

作者： chen sharon Centre for Infectious Diseases and Microbiology Laboratory Services Westmead HospitalDarcy RoadWestmeadNew South WalesA ustralia

To study the epidemiology of yeast pathogens and their antifungal susceptibility,we conducted active laboratory-based surveillance for isolates from patients with invasive infections across China from August 2010- July ***-NET is the first nationwide invasive fungal infection surveillance study in China that covered 23 tertiary hospitals in 15 provinces and *** is important to note that,only yeasts that isolated from blood samples or other sterile body sites were collected,whilst sputum and urine were excluded from the present study. DNA sequencing methods were used to define species,and susceptibility to fluconazole and voriconazole performed by the Clinical Laboratory Standards Institute M-44A2 disk diffusion *** 1 243 yeasts,Candida spp. comprised 91.3%of strains,followed by Cryptococcus neoformans(7.0%;69.7%of isolates were from cerebrospinal fluid) and other non-candidal yeasts(1.7%).Bloodstream isolates comprised 39.4%of strains,isolates from ascitic fluid comprised 24.9%.Amongst Candida,Candida albicans was the commonest species which accounted for 44.7%,followed by Candida tropicalis(17.5%) Candida parapsilosis sensu strict(13%) and Candida glabrata sensu strict(9.3%).In general,most yeasts were susceptible to fluconazole(FLU) and voriconazole(VOR). The resistant rate to FLU and VOR for the most common Candida *** Candida albicans(0.4%,0.4%) Candida tropicalis(3.7%,3.7%),Candida parapsilosis sensu strict(7.5%,3.1%) and Candida glabrata sensu strict (16.5%,15.7%).Most resistant strains showed cross-resistant to FLU and *** resistance rate to FLU and VOR for uncommon Candida/non-candidal yeasts(*** pathogens and their antifungal susceptibilities in China.

关键词： China hospital invasive fungal surveillance net(CHIF-NET) Antifungal susceptibility,Molecular identification Disk diffusion

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A PCR-based intergenic spacer(IGS ) region typing method for the identification and subtyping of nocardia species

A PCR-based intergenic spacer(IGS ) region typing method for...

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2012全国临床微生物与感染免疫学术研讨会

作者： Wehrhahn Micheal chen sharon Westmead Hospital

While 16S ribosomal RNAsequence-based identification of Nocardia species has become the gold standard,it is not without its *** evaluated a novel approach encompassing the amplification of the Nocardia 16S-23S rRNA intergenic spacer(IGS) region followed by fragment analysis by capillary gel electrophoresis (CGE) of the amplified product for species identification of *** hundred and forty-five Nocardia isolates(19 species) and four non-nocardiae aerobic actinomycetes were *** testing was performed in a subset(21%) of ***-five different electropherograms were identified,with heterogeneity within species a general *** common Nocardia species(*** cyriacigeorgica,N. nova,***),2-3 dominant electropherogram subgroups were *** only a minority of the different Nocardia species(8/19;42%) contained isolates displaying unique fragment sizes that were predictive of a particular species,virtually all isolates(142/145;98%) could be assigned to the correct species using IGS typing based on the number and size of amplified *** median number of fragments for each isolate was two(range: one to five) with only a minority(17%) having a single fragment *** majority(93%) of amplified fragments were between 408-461 *** technique was also non-operator dependent and highly reproducible and was quicker and less expensive than 16S *** summary,PCR-based IGS typing is relatively simple,accurate, reproducible and cost-effective and offers a potential alternative to 16S rRNA sequencing for identifying and subtyping Nocardia isolates.

关键词： Nocardia 16S-23S rRNA Intergenic Spacer(IGS) Capillary gel electrophoresis(CGE) PCR

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滚环扩增技术鉴定念珠菌、曲霉和赛多孢子菌

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中华皮肤科杂志 2009年第8期42卷 582-583页

作者：周小勇孔繁荣 Tania Sorrell 王辉段逸群 sharon chen 武汉市第一医院感染性皮肤病研究室 430022 澳大利亚悉尼大学韦斯米德医院感染性疾病和微生物中心

由于机会感染真菌对抗真菌药物的敏感性存在很大的差异，因此，及时鉴定机会感染真菌到种的水平对于后续临床治疗具有重要意义。为了克服传统培养鉴定方法耗时和敏感性差的缺陷，针对真菌核糖体DNA基因复合体ITS区，在PCR技术基础上建... 详细信息

由于机会感染真菌对抗真菌药物的敏感性存在很大的差异，因此，及时鉴定机会感染真菌到种的水平对于后续临床治疗具有重要意义。为了克服传统培养鉴定方法耗时和敏感性差的缺陷，针对真菌核糖体DNA基因复合体ITS区，在PCR技术基础上建立了许多分子生物学鉴定技术，如多重和（或）实时PCR、DNA序列分析和探针相关技术。滚环扩增技术能检测到数个碱基甚至单个碱基的差异，显示出很好的敏感性和特异性。我们采用滚环扩增技术，鉴定机会感染真菌包括除白念珠菌和烟曲霉以外的念珠菌和曲霉，以及赛多孢子菌。

关键词：技术鉴定扩增技术白念珠菌烟曲霉孢子抗真菌药物 DNA序列分析机会感染

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新加坡满是富裕的木头人

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商业周刊（中文版） 2012年第23期 17-18页

作者： Bruce Einhorn sharon chen

一项盖洛普民意测验显示，这个城市国家的人最缺乏情感“我们从小就被教导要不断前进，别没事就大惊小怪”

关键词：新加坡富裕民意测验城市国家盖洛普

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解封后的武汉生活已不同以往

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商业周刊（中文版） 2020年第6期 8-10页

作者： sharon chen Claire Che 杨飞(译) 不详

在居家隔离两个多月之后,随着新增感染病例从2月中旬的每日几千例降至每周仅数例,1100万武汉居民当中的大多数人现在已经可以自由外出了。但是,对餐馆老板熊飞(Xiong Fei)来说,这座最早暴发新冠疫情的中国城市的解封并没有让他感到轻松... 详细信息

在居家隔离两个多月之后,随着新增感染病例从2月中旬的每日几千例降至每周仅数例,1100万武汉居民当中的大多数人现在已经可以自由外出了。但是,对餐馆老板熊飞(Xiong Fei)来说,这座最早暴发新冠疫情的中国城市的解封并没有让他感到轻松,只是带来了一系列新的挑战。

关键词：解封餐馆老板新的挑战武汉

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米替福新对常见皮肤癣菌体外抑菌活性的研究

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华中医学杂志 2007年第5期31卷 341-342,345页

作者：童中胜段逸群王玮蓁江萍 sharon C.A.chen 武汉市第一医院皮肤科武汉430022 澳大利亚悉尼大学附属Westmead医院微生物感染性疾病研究中心

目的了解米替福新对常见皮肤癣菌的体外抑菌活性。方法参照美国临床和实验室标准化研究所（CLSI）制定的M38-A方案测定77株常见皮肤癣菌（含三属即毛癣菌属、小孢子菌属和表皮癣菌属）的最低抑菌浓度（MIC）。结果米替福新对常见皮肤癣... 详细信息

目的了解米替福新对常见皮肤癣菌的体外抑菌活性。方法参照美国临床和实验室标准化研究所（CLSI）制定的M38-A方案测定77株常见皮肤癣菌（含三属即毛癣菌属、小孢子菌属和表皮癣菌属）的最低抑菌浓度（MIC）。结果米替福新对常见皮肤癣菌均有较好的抑菌作用,MIC90为1.0μg/ml,MIC几何均数为0.617μg/ml,MIC范围0.25-2.0μg/ml。结论米替福新对常见皮肤癣菌各属均有较好的抑菌作用。

关键词：抗真菌药物敏感性试验体外米替福新伊曲康唑特比萘芬皮肤癣菌

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滚环扩增技术鉴定念珠菌、曲霉和赛多孢子菌

滚环扩增技术鉴定念珠菌、曲霉和赛多孢子菌

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2009全国中西医结合皮肤性病学术会议

作者：周小勇孔繁荣 Tania Sorrell 王辉 sharon chen 武汉市第一医院感染性皮肤病研究室澳大利亚悉尼大学韦斯米德医院感染性疾病和微生物中心

由于机会感染真菌对抗真菌药物的敏感性存在很大的差异,因此及时鉴定机会感染真菌到种的水平,对于后续临床治疗具有重要意义。为了克服传统培养鉴定方法耗时和敏感性差的缺陷,针对真菌核糖体DNA基因复合体ITS区,在PCR技术基础上建立了...

由于机会感染真菌对抗真菌药物的敏感性存在很大的差异,因此及时鉴定机会感染真菌到种的水平,对于后续临床治疗具有重要意义。为了克服传统培养鉴定方法耗时和敏感性差的缺陷,针对真菌核糖体DNA基因复合体ITS区,在PCR技术基础上建立了许多分子生物学鉴定技术如:多重和/或实时PCR、DNA序列分析和探针相关技术。

关键词：

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Transdermal delivery of Chinese herbal medicine extract using dissolvable microneedles for hypertrophic scar treatment

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Acta Pharmaceutica Sinica B 2021年第9期11卷 2937-2944页

作者： Xiaoyu Ning Christian Wiraja Wan Ting sharon Chewa chen Fan chenjie Xu NTU Institute for Health Technologies Interdisciplinary Graduate GroupNanyang Technological UniversitySingapore 639798Singapore School of Chemical and Biomedical Engineering Nanyang Technological UniversitySingapore 637459Singapore Skin Research Institute of Singapore 8A Biomedical GroveSingapore 138648Singapore Department of Biomedical Engineering City University of Hong KongHong Kong SAR 999077China

Hypertrophic scars are unfavorable skin diseases characterized by excessive collagen *** systemic treatments exist in clinic to manage hypertrophic scars,they pose significant side effects and tend to lose efficacy over prolonged *** Chinese medicine(TCM)offers as a promising candidate to treat pathological scars.A large number of TCMs have been studied to show anti-scarring effect,however,the natural barrier of the skin impedes their penetration,lowering its therapeutic ***,we reported the use of dissolvable hyaluronic acid(HA)microneedles(MNs)as a vehicle to aid the transdermal delivery of therapeutic agent,a model TCM called shikonin for the treatment of hypertrophic ***,shikonin was mixed with HA to make MNs with adequate mechanical strength for skin penetration,making its dosage controllable during the fabrication *** therapeutic effect of the shikonin MNs was studied in vira using HSFs and then further verified with quantitative reverse transcriptase polymerase chain *** data suggest that the shikonin HA MNs significantly reduce the viability and proliferation of the HSFs and downregulate the fibrotic-related genes(i.e.,TGFβ1,FAP-αand COL1 A1).Furthermore,we observed a localized therapeutic effect of the shikonin HA MNs that is beneficial for site-specific treatment.

关键词： Traditional Chinese medicine Shikonin Hyaluronic acid Microneedle Hypertrophic scarring Drug delivery Transdermal delivery Skin

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IGF-1R as an anti-cancer target—trials and tribulations

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Chinese Journal of Cancer 2013年第5期32卷 242-252页

作者： Helen X. chen Elad sharon Cancer Therapy Evaluation Program National Cancer Institute

Type I insulin-like growth factor receptor (IGF-1R) has long been recognized for its role in tumorigenesis and growth, but only recently have the tools for targeting the IGF pathway become available. More than 10 IGF/IGF-1R inhibitors have entered clinical trials, and these belong to three main classes: (1) monoclonal antibodies against IGF-1R, (2) monoclonal antibodies against IGF-1R ligands (IGF-1 and IGF- 2), and (3) IGF-1R tyrosine kinase inhibitors. These IGF-1R-targeting agents share common effects on IGF-1R signaling but differ in mechanisms of action, spectrum of target inhibition, and pharmacological features. Clinical activity of IGF-1R inhibitors has been demonstrated with sustained responses in a small number of patients with select tumor types, such as Ewing sarcoma and thymoma. However, many large clinical trials involving patients with adult tumors, including non-small cell lung cancer, breast cancer, and pancreatic cancer, failed to show clinical benefit in the overall patient population. Possible reasons for failure include the complexity of the IGF-1R/insulin receptor system and parallel growth and survival pathways, as well as a lack of patient selection markers. While IGF-1R remains a valid target for selected tumor types, identification of predictive markers and rational combinations will be critical to success in future development.

关键词： IGF-1 酪氨酸激酶抑制剂胰岛素样生长因子生长因子受体单克隆抗体肿瘤发生临床试验抗癌

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Proposed nomenclature for Pseudallescheria,Scedosporium and related genera

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Fungal Diversity 2014年第4期67卷 1-10页

作者： Michaela Lackner G.Sybren de Hoog Liyue Yang Leandro Ferreira Moreno Sarah A.Ahmed Fritz Andreas Josef Kaltseis Markus Nagl Cornelia Lass-Florl Brigitte Risslegger Gunter Rambach Cornelia Speth Vincent Robert Walter Buzina sharon chen Jean-Philippe Bouchara Jose F.Cano-Lira Josep Guarro Josepa Gené Fabiola Fernández Silva Rosa Haido Gerhard Haase Vladimir Havlicek Dea Garcia-Hermoso Jacques F.Meis Ferry Hagen Martin Kirchmair Johannes Rainer Katharina Schwabenbauer Mirjam Zoderer Wieland Meyer Felix Gilgado Vania A.Vicente Elena Piecková Monika Regenermel Peter-Michael Rath Joerg Steinmann Xisto Wellington de Alencar Francoise Symoens Kathrin Tintelnot Krzysztof Ulfig Aristea Velegraki Anna Maria Tortorano Sandrine Giraud Sara Mina Kinga Rigler-Hohenwarter Fernando L.Hernando Andoni Ramirez-Garcia Aize Pellon Jashanpreet Kaur Eliana Barreto Bergter Jardel Vieira de Meirelles Ingrid Dutra da Silva Laurence Delhaes Ana Alastruey-Izquerdo Ruo-yu Li Qiaoyun Lu Tarek Moussa Omar Almaghrabi Hassan Al-Zahrani Gen Okada Shuwen Deng Wangqing Liao Jingsi Zeng Jouni Issakainen Livia Cristina Liporagi Lopes Division of Hygiene and Medical Microbiology Innsbruck Medical UniversityInnsbruckAustria CBS Fungal Biodiversity Centre UtrechtThe Netherlands Institute for Biodiversity and Ecosystem Dynamics University of AmsterdamAmsterdamThe Netherlands Sun Yat-Sen Memorial Hospital Sun Yat-Sen UniversityGuangzhouChina Medical University of Graz GrazAustria Centre for Infectious Diseases and Microbiology Westmead HospitalWestmeadAustralia Laboratoire de Parasitologie-Mycologie Centre Hospitalier UniversitaireAngersFrance Unitats de Microbiologia i d’Anatomia Patològica Facultat de Medicina i Ciències de la Salut IISPVUniversitat Rovira i VirgiliReusSpain Universidade Federal do Estado do Rio de Janeiro Rio de JaneiroBrazil Institute of Medical Microbiology University Hospital RWTHAachenGermany Institute of Microbiology Academy of SciencesPragueCzech Republic Institut Pasteur Unitéde Mycologie MoléculaireCentre National de Référence Mycoses Invasives et AntifongiquesParisFrance Department of Medical Microbiology and Infectious Diseases Canisius-Wilhelmina HospitalNijmegenThe Netherlands Department of Medical Microbiology RadboudumcNijmegenThe Netherlands Institute of Microbiology eopold-Franzens-University InnsbruckInnsbruckAustria Molecular Mycology Research Laboratory Centre for Infectious Diseases and MicrobiologySydneyMedical School—Westmead HospitalMarie Bashir Institute for Infectious Diseases and BiosecurityThe University of SydneyWestmead Millennium InstituteWestmeadNSWAustralia Basic Pathology Department Federal University of ParanáStateCuritibaParanáBrazil Fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq) BrasíliaBrazil Institute of Preventive and Clinical Medicine BratislavaSlovakia Institut für Hygiene und Mikrobiologie Landesklinikum St.Pölten St.PöltenAustria Institute of Medical Microbiology University Hospital EssenEssenGermany Nursing School of Unigranrio Rio de JaneiroBrazil Scientific Institute of Public Health Culture Collectio

As a result of fundamental changes in the International Code of Nomenclature on the use of separate names for sexual and asexual stages of fungi,generic names of many groups should be *** of the ECMM/ISHAM working group on Pseudallescheria/Scedosporium infections herein advocate a novel nomenclature for genera and species in Pseudallescheria,Scedosporium and allied *** generic names Parascedosporium,Lomentospora,Petriella,Petriellopsis,and Scedosporium are proposed for a lineage within Microascaceae with mostly Scedosporium anamorphs producing slimy,annellidic *** that Scedosporium has priority over Pseudallescheria and that Scedosporium prolificans is phylogenetically distinct from the other Scedosporium species,some name changes are *** minutispora and Petriellidium desertorum are renamed as Scedosporium minutisporum and ***,*** prolificans is renamed as Lomentospora prolificans.

关键词： Graphium Lomentospora Petriella Scedosporium apiospermum complex Microascaceae Nomenclature

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