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双乾肉羊PDK4基因的组织表达差异及多态性分析

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黑龙江畜牧兽医 2024年第1期 47-53页

作者：缪立生肖成杨少滢栾祜赵文军沈宏旭刘宇曹阳巩俊明无吉林省农业科学院长春132101 吉林农业大学动物科学技术学院长春130118 双辽市动物疫病预防控制中心吉林双辽136400 延边大学农学院吉林延吉136200

为了研究丙酮酸脱氢酶激酶4(pyruvate dehydrogenase kinase 4,PDK4)基因在双乾肉羊不同组织中的表达差异,以及基因外显子多态性对肉质性状的影响,试验以45只健康的8月龄双乾肉羊公羊为研究对象,屠宰后采集心脏、肝脏、脾脏、肺脏、肾... 详细信息

为了研究丙酮酸脱氢酶激酶4(pyruvate dehydrogenase kinase 4,PDK4)基因在双乾肉羊不同组织中的表达差异,以及基因外显子多态性对肉质性状的影响,试验以45只健康的8月龄双乾肉羊公羊为研究对象,屠宰后采集心脏、肝脏、脾脏、肺脏、肾脏、十二指肠及背最长肌,利用PCR法与Sanger测序法结合的方式检测PDK4基因外显子中的单核苷酸多态性位点(SNP),并与肉质性状数据进行关联分析。结果表明:PDK4基因广泛分布于各组织中,在十二指肠组织中的相对表达量最高。PDK4基因第1外显子存在2个SNP位点,分别为Chr 4.14723148 T>C突变、Chr 4.14723208 T>C突变。Chr 4.14723148 T>C存在2种基因型,分别为CC和CT基因型;Chr 4.14723208 T>C存在3种基因型,分别是TT、TC和CC基因型。突变基因型分布符合哈代温伯格(Hardy-Weinberg)定律,突变个体在群体中处于稳定状态。Chr 4.14723148 T>C和Chr 4.14723208 T>C位点的多态性信息含量分别为0.94和0.61,均为高度多态(P>0.5),Chr 4.14723148 T>C突变中CC基因型为优势基因型,其个体压榨水分率显著低于CT基因型个体(P<0.05)。说明双乾肉羊群体稳定存在Chr 4.14723148突变位点,并且CC优势基因型个体肌肉水分保持能力更强,正向影响肉质性状。

关键词：双乾肉羊 PDK4基因突变位点肉质性状组织表达谱

鸭短喙矮小综合征灭活疫苗的研制及免疫效力评价

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中国兽医学报 2024年第2期44卷 264-267页

作者：陈仕龙程晓霞肖世峰江丹丹林锋强王劭朱小丽俞伏松邹敏陈少莺福建省农业科学院畜牧兽医研究所福建福州350000 福建省畜禽疫病防治工程技术研究中心福建福州350013 青岛易邦生物工程有限公司山东青岛266000

鸭短喙矮小综合征(short beak and dwarfism syndrome,SBDS)是近年来在我国鸭群中流行的一种新传染病,为研制SBDS灭活疫苗,本研究将SBDSV M15株在番鸭胚增殖,收获病毒尿囊液经灭活后制备了3批灭活疫苗,并对疫苗的安全性、免疫原性及免... 详细信息

鸭短喙矮小综合征(short beak and dwarfism syndrome,SBDS)是近年来在我国鸭群中流行的一种新传染病,为研制SBDS灭活疫苗,本研究将SBDSV M15株在番鸭胚增殖,收获病毒尿囊液经灭活后制备了3批灭活疫苗,并对疫苗的安全性、免疫原性及免疫效力进行检验。结果显示,3日龄樱桃谷鸭超剂量接种1.0 mL/羽,未见不良反应,表明疫苗的安全性好;3批灭活疫苗免疫20日龄雏半番鸭,免疫后28 d血清的胶乳凝集抑制试验(LPAI)抗体效价均值为4.3 log_(2);130日龄樱桃谷鸭种鸭免疫1次灭活疫苗后60 d血清LPAI抗体平均效价为6.9 log_(2);种鸭免疫后60 d所产种蛋卵黄抗体的LPAI效价均值为6.5 log_(2);对种鸭免疫后的3日龄子代雏鸭进行攻毒获得100%免疫保护。结果表明,本研究研制的SBDS灭活疫苗具有良好的免疫原性,种鸭1次免疫后可提供子代雏鸭保护性母源抗体,保护雏鸭度过最易感的危险窗口期,为SBDS的防控提供了有效手段。

关键词：鸭短喙矮小综合征灭活疫苗抗体效价母源抗体免疫效力

Radiation balance and the response of albedo to environmental factors above two alpine ecosystems in the eastern Tibetan Plateau

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Research in Cold and Arid Regions 2017年第2期9卷 142-150页

作者： shaoying Wang yu Zhang ShiHua Lyu Lunyu Shang YouQi Su HanHui Zhu Key Laboratory of Land Surface and Climate Change in Cold and Arid Regions Northwest Institute of Eco-Environment and Resources Chinese Academy of Sciences Lanzhou Gausu 730000 China College of Atmospheric Sciences Chendu University of Information Technology Chengdu Sichuan 610225 China

Understanding the energy balance on the Tibetan Plateau is important for better prediction of global climate change. To characterize the energy balance on the Plateau, we examined the radiation balance and the response of albedo to environmental factors above an alpine meadow and an alpine wetland surfaces in the eastern Tibetan Plateau, using 2014 data. Although our two sites belong to the same climatic background, and are close geographically, the annual incident solar radiation at the alpine meadow site(6,447 MJ/(m2·a)) was about 1.1 times that at the alpine wetland site(6,012 MJ/(m2·a)),due to differences in the cloudiness between our two sites. The alpine meadow and the alpine wetland emitted about 38%and 42%, respectively, of annual incident solar radiation back into atmosphere in the form of net longwave radiation; and they reflected about 22% and 18%, respectively, of the annual incident solar radiation back into atmosphere in the form of shortwave radiation. The annual net radiation was 2,648 and 2,544 MJ/(m2·a) for the alpine meadow site and the alpine wetland site, respectively, accounting for only about 40% of the annual incident solar radiation, significantly lower than the global mean. At 30-min scales, surface albedo exponentially decreases with the increase of the solar elevation angle; and it linearly decreases with the increase of soil-water content for our two sites. But those relationships are significantly influenced by cloudiness and are site-specific.

关键词： Tibetan Plateau radiation balance surface albedo solar elevation angle soil water content

基于金课构建“食品微生物实验技术”层级立体化教学模式

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食品工业 2024年第9期45卷 241-244页

作者：于有伟张少颖张秀红徐建国崔美林李艳山西师范大学食品科学学院太原030031

为有效达成“食品微生物实验技术”的课程目标,教学团队按照金课两性一度的标准,在教学过程中构建层级立体化教学模式。教学团队通过优化实验内容,逐级提高复杂程度;联动实施实验,促使本科生强化训练操作技能;面向新工科产业实践,培养... 详细信息

为有效达成“食品微生物实验技术”的课程目标,教学团队按照金课两性一度的标准,在教学过程中构建层级立体化教学模式。教学团队通过优化实验内容,逐级提高复杂程度;联动实施实验,促使本科生强化训练操作技能;面向新工科产业实践,培养本科生创新能力;与第二课堂有机结合,培养学生实践能力等,有效构建了建层级立体化教学模式。这种教学模式有助于培养学生深入领会理论知识,扎实掌握操作技能,塑造良好的责任担当意识。层级立体化教学模式实现新工科背景下“食品微生物实验技术”课程培养高素质食品科技人才的教学目标。

关键词：食品微生物实验技术金课两性一度层级立体化教学模式

产教融合视角下“山西特色酿造食品”课程的构建与探索

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农产品加工 2024年第16期 130-133,136页

作者：张秀红崔美林王佳丽赵微张少颖于有伟山西师范大学食品科学学院山西太原030002 山西电子科技学院生物与食品工程学院山西临汾041000

清香汾酒和山西老陈醋历史悠久,工艺精湛,是极具地方特色的酿造食品。为了增强带入感,突出产教融合特征,食品类专业的选修课“山西特色酿造食品”建设过程中,教学内容融入产业文化、详解酿造工艺及重要参数,与国内其他同类名优食品比较... 详细信息

清香汾酒和山西老陈醋历史悠久,工艺精湛,是极具地方特色的酿造食品。为了增强带入感,突出产教融合特征,食品类专业的选修课“山西特色酿造食品”建设过程中,教学内容融入产业文化、详解酿造工艺及重要参数,与国内其他同类名优食品比较以便互相借鉴;在课堂教学方面,通过大量实际生产的照片视频等进行沉浸式教学、透过工艺参数理解酿造过程控制、结合生产工作任务学习工艺流程,同时融入课程思政,感受大国工匠精神。以期为产教融合类课程的建设和应用型人才培养提供参考。

关键词：汾酒山西老陈醋产教融合

Winter estimation of surface roughness length over eastern Qinghai-Tibetan Plateau

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Research in Cold and Arid Regions 2017年第2期9卷 151-157页

作者： Lunyu Shang yu Zhang ShiHua Lyu shaoying Wang YinHuan Ao SiQiong Luo ShiQiang Chen Key Laboratory of Land Surface Process and Climate Change in Cold and Arid Regions Northwest Institute of Eco-Environment and Resources Chinese Academy of Sciences Lanzhou Gansu 730000 China College of Atmospheric Science Chengdu University of Information Technology Chengdu Sichuan 610225 China

Based on the Monin-Obulchov similarity theory, a scheme was developed to calculate surface roughness length. Surface roughness length over the eastern Qinghai-Tibetan Plateau during the winter season was then estimated using the scheme and eddy covariance measurement data. Comparisons of estimated and measured wind speeds show that the scheme is feasible to calculate surface roughness length. The estimated roughness lengths at the measurement site during unfrozen, frozen and melted periods are 3.23x10(-3), 2.27x10(-3) and 1.92x10(-3) m, respectively. Surface roughness length demonstrates a deceasing trend with time during the winter season. Thereby, setting the roughness length to be a constant value in numerical models could lead to certain degree of simulation errors. The variation of surface roughness length may be caused by the change in land surface characteristic.

关键词： Qinghai-Tibetan Plateau surface roughness length Monin-Obuichov similarity theory least square fitting

采前1-甲基环丙烯处理对货架期‘Bartlett’梨虎皮病和抗氧化特性的影响

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食品科学 2021年第1期42卷 250-256页

作者：李英丽张少颖董宇河北农业大学园艺学院河北省梨工程技术研究中心河北保定071001 美国俄勒冈州立大学哥伦比亚农业研究与推广中心美国俄勒冈胡德里弗97031 山西师范大学食品科学学院山西临汾041004

以‘Bartlett’梨为实验材料,研究了采前喷施300 mg/L 1-甲基环丙烯(1-methylcyclopene,1-MCP)和蒸馏水(对照)对货架期梨果实虎皮病以及相关抗氧化物质含量、抗氧化酶活力的影响,为今后研发适宜的调控果实虎皮病技术提供依据。结果表明... 详细信息

以‘Bartlett’梨为实验材料,研究了采前喷施300 mg/L 1-甲基环丙烯(1-methylcyclopene,1-MCP)和蒸馏水(对照)对货架期梨果实虎皮病以及相关抗氧化物质含量、抗氧化酶活力的影响,为今后研发适宜的调控果实虎皮病技术提供依据。结果表明:与对照相比,采前15 d 1-MCP处理能降低货架期果实虎皮病病情指数,推迟虎皮病发生的时间,延长贮藏期1个月,降低梨在室温下放置过程中呼吸速率、乙烯生成速率和果皮α-法尼烯、共轭三烯含量,推迟乙烯高峰的出现;显著提高中后期果皮1,1-二苯基-2-三硝基苯肼自由基清除能力以及花青苷、总酚和总黄酮含量,抑制抗坏血酸过氧化物酶和过氧化氢酶活力的下降,提高后期过氧化物酶活力,降低膜脂过氧化,减少丙二醛的积累。

关键词： ‘Bartlett’梨果实 1-甲基环丙烯虎皮病抗氧化物质抗氧化酶

Numerical Optimization of Tunnel-recombination Junction and Optical Absorption Properties of a-Si:H/a-SiGe:H Double-junction Solar Cell

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Journal of the Chinese Ceramic Society 2015年第1期 1-11页

作者： KE shaoying WANG Chong PAN Tao WANG Zhaoqing YANG Jie YANG yu Institute of Optoelectronic Information Materials Yunnan University National Laboratory for Infrared Physics Shanghai Institute of Technical Physics Chinese Academy of Science

The tunnel-recombination junction(TRJ) and optical absorption properties of a-Si:H/a-Si Ge:H double-junction solar cell were calculated by means of one dimensional simulator named AMPS-1D at the radiation of AM1.5G with a power density of 100 m W/cm2. Since the TRJ is the core component of the tandem solar cell, the optical absorption of the sub-cells and the electronic transport properties at the interface of the sub-cells are affected by the thickness and doping concentration of the TRJ. As a result, the TRJ parameters were optimized. The numerical results indicate that the maximum conversion efficiency(Eff) of 9.862% can be obtained when the thickness and doping concentration of the TRJ are 10 nm and 5*1019 cm–3, respectively. Based on the analysis of the contour map of short circuit current density, the optimal current matching can be achieved for 130 nm-thick top i-layer and 250 nm-thick bottom i-layer. In addition, four kinds of TRJ structures were also simulated for the comparison purpose. According to the calculated resistivity and band structures of the four TRJs, the efficiency of the solar cell with n-type μc-Si:H layer and p-type a-Si:H layer in TRJ structure is greater than that with other TRJ structures. It is assumed that the effect of the band offset that results in the formation of triangular barrier and backscattering behavior at the edge of the TRJ could be responsible to this phenomenon.

关键词： a-Si:H/a-Si Ge:H tandem solar cell tunnel-recombination junction current matching band offset

Meeting 24-h Movement Guidelines is Related to Better Academic Achievement:Findings from the YRBS 2019 Cycle

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International Journal of Mental Health Promotion 2022年第1期24卷 13-24页

作者： shaoying Liu Qian yu Md Mahbub Hossain Scott Doig Ran Bao Yaping Zhao Jin Yan Xun Luo Jiaxuan Yang Arthur F.Kramer Liye Zou College of Sports Science Haikou University of EconomicsHaikouChina College of Sports Science Jishou UniversityJishouChina Exercise Psychophysiology Laboratory Institute of KEEP Collaborative InnovationSchool of PsychologyShenzhen UniversityShenzhenChina Department of Health Promotion and Community Health Sciences School of Public HealthTexas A&M UniversityCollege StationUSA College of Education and Health Professions Limestone UniversitySouth CarolinaUSA School of Physical Education and Sport Training Shanghai University of SportShanghaiChina The Library of Shandong University of Sport ShandongChina Priority Research Centre in Physical Activity and Nutrition School of EducationUniversity of NewcastleCallaghanAustralia Beckman Institute University of IllinoisUrbanaIllinoisUSA Canter for Cognitive&Brain Health Northeastern UniversityBostonUSA

This research is designed to investigate the relationship between the 24-h movement guidelines(24-HMG)and self-reported academic achievement(AA)using nationally representative data derived from the 2019 *** Youth Risk Behaviour Survey.A multiple-stage cluster sampling procedure has been adopted to ensure a representative sample(N=9127 adolescents;mean age=15.7 years old;male%=49.8%).Logistic regression has been adopted to obtain the odds ratio(OR)regarding the associations between adherence to 24-HMG and AA while controlling for ethnicity,body mass index,sex and *** prevalence of meeting the 24-h movement guidelines in isolation and combination varied greatly(physical activity=23.3%,screen time=32.5%,sleep=22.3%,and 24-HMG=2.8%),while the percentage of highest-class AA was 42.5%.Compared with the situation when none of 24-HMG is met,the achievement of any of the combined guidelines(except for meeting the physical activity guidelines)was significantly associated with higher odds of achieving first-class *** the sleep guideline had 1.42 times increased likelihood to achieve highest-class AA as compared with not meeting the sleep *** screen time guidelines and physical activity guidelines,respectively,were 1.32 and 1.13 times more likely to report first-class AA;but meeting the guidelines of physical activity was not significantly related to *** the 24-HMG had the highest odds of achieving first-class AA(OR=2.01,95%CI:1.47-2.73).In both sexes,adolescents who met 24-HMG self-reported better AA(boys OR=2.05,95%CI:1.34-3.15;girls OR=2.26,95%CI:1.36-3.76).Significant relationships were observed in adolescents from 9-10th grade,but not higher *** research findings suggest that optimal movement behaviours can be seen as an important element to better academic achievement among *** studies can adopt our discoveries to promote adolescents’academic achievement through implementing optimal 24-h movement behaviour

关键词： Sedentary behaviour physical activity sleep academic performance high school students U.S.