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International Journal of Minerals,Metallurgy and Materials 2023年第6期30卷 1207-1216页

作者： Shuai Zhang Yanling Zhang shaowen wu State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China

Augite-based glass ceramics were synthesised using ZnO,FeO,and Fe_(2)O_(3)as additives,and the spinel formation,matrix structure,crystallisation thermodynamics,and physicochemical properties were investigated.The results showed that oxides resulted in numerous preliminary spinels in the glass matrix.FeO,ZnO,and Fe_(2)O_(3)influenced the formation of spinel,while FeO simplified the glass network.FeO and ZnO promoted bulk crystallisation of the parent glass.After adding oxides,the grains of augite phase were refined,and the relative quantities of augite crystal planes were also influenced.All samples displayed good mechanical properties and chemical stability.The 2wt%ZnO-doping sample displayed the maximum flexural strength(170.3 MPa).Chromium leaching amount values of all the samples were less than the national standard(1.5 mg/L),confirming the safety of the materials.In conclusion,an appropriate amount of zinc-containing raw material is beneficial for the preparation of augite-based glass ceramics.

关键词： spinel network structure thermodynamics microstructure glass ceramics

Changes in cesarean section rates and its indications among primiparous women during each implementation phase of Chinese two-child policy:a retrospective study

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Chinese Medical Journal 2022年第18期135卷 2264-2266页

作者： Yumei Cheng Shaofei Su shaowen wu Department of Perinatal Medicine Beijing Obstetrics and Gynecology HospitalCapital Medical UniversityBeijing Maternal and Child Health Care HospitalBeijing 100026China Department of Central Laboratory Beijing Obstetrics and Gynecology HospitalCapital Medical UniversityBeijing Maternal and Child Health Care HospitalBeijing 100026China

To the Editor:Cesarean section(CS)is among the most important and safe procedures in obstetrics.When medically indicated,CS can be a life-saving intervention.During the Chinese one-child policy,couples tended to choose CS for various reasons.In 2010,the CS rate in China rose to among the world’s highest.^([1])Yet,CS can also have various adverse maternal and neonatal consequences.Maternal mortality and maternal and infant complications are higher with CS compared with vaginal delivery.[2]Over the past several decades,CS rates have increased sharply worldwide,and controlling these rates has become among the most prominent obstetric issues.

关键词： section policy among

Fast electrothermal coupling calculation method for supporting digital twin construction of electrical equipment

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High Voltage 2023年第2期8卷 390-400页

作者： Xiaosong Zhu Youyuan Wang Ruixiao Meng Shi Long shaowen wu State Key Laboratory of Power Transmission Equipment and System Security and New Technology(Chongqing University) ChongqingChina

The failure of electrical equipment directly or indirectly caused by overheating has become one of the main reasons for equipment accidents.The real-time condition monitoring method of electrical equipment based on digital twin(DT)has received extensive attention and is considered as a technology with great engineering values and excellent application prospects.However,the current calculation of DT mostly relies on the traditional finite element method(CFEM).This method becomes less computationally efficient as the size of the DT increases,especially for electrical equipment with high complexity.It is difficult to meet the requirements real-time calculation in DT.Therefore,starting from the algorithm optimization and parallel architecture,based on the weighted residual method and stabilised conforming nodal integration,a novel discrete method of electrothermal coupling equation is proposed and the data storage structure of the algorithm from the bottom layer is redesigned,and the corresponding GPU and multi-core CPU parallel framework are proposed.Finally,taking the high voltage bushing as an example,the correctness of the method in this paper is verified by the CFEM code and commercial software ABAQUS.Under the same grid and accuracy requirement,the calculation time is shortened by at least five times than ABAQUS.And the method is easy to extend to other types of multi-physics calculations.

关键词： electrical coupling method

Highly Efficient Photothermocatalytic CO_(2) Reduction in Ni/Mg-Doped Al_(2)O_(3) with High Fuel Production Rate, Large Light-to-Fuel Efficiency, and Good Durability

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Energy & Environmental Materials 2022年第2期5卷 582-591页

作者： Xin Tan shaowen wu Yuanzhi Li Qian Zhang Qianqian Hu Jichun wu An Zhang Yongdi Zhang State Key Laboratory of Silicate Materials for Architectures Wuhan University of TechnologyWuhan 430070China

A novel nanocomposite of Ni nanoparticles loaded on Mg-doped Al_(2)O_(3)(Ni/Mg-Al_(2)O_(3))was prepared.By photothermocatalytic CO_(2) reduction with methane(CRM)merely using focused UV-vis-IR illumination on Ni/Mg-Al_(2)O_(3),high production rates of H_(2)(r_(H2),69.71 mmol min^(−1) g^(−1))and CO(rCO,74.57 mmol min^(−1) g^(−1))and an extremely large light-to-fuel efficiency(η,32.9%)are acquired.High rH2 and rCO(51.07 and 59.66 mmol min^(−1) g^(−1))and a largeη(32.5%)are acquired even by using focusedλ>560 nm vis-IR illumination.Ni/Mg-Al_(2)O_(3) shows good durability for photothermocatalytic CRM due to the side reaction of carbon deposition being enormously inhibited in comparison with a reference catalyst of Ni nanoparticles loaded on Al_(2)O_(3).The enormous carbon deposition inhibition is ascribed to the presence of a fence of CO_(2) molecules(strongly adsorbed on Mg-doped Al_(2)O_(3))around Ni nanoparticles,which block the polymerization and growth of carbon species to nanofibers by promoting the oxidation of carbon species formed by CH_(4) dissociation.The high photothermocatalytic activity of Ni/Mg-Al_(2)O_(3) arises from efficient light-driven thermocatalytic CRM.A photoactivation is found to considerably raise the photothermocatalytic activity of Ni/Mg-Al_(2)O_(3) because of the apparent activation energy(Ea)being substantially decreased upon focused illumination.The Ea reduction is associated with the rate-determining steps of CRM(e.g.,CH_(4) dissociation and the oxidation of carbon species)being accelerated upon focused illumination.

关键词： CO_(2)reduction light-to-fuel efficiency Ni/Mg-Al_(2)O_(3)nanocomposite photocatalytic photothermocatalytic

Methionine addition improves the acid tolerance of Lactiplantibacillus plantarum by altering cellular metabolic flux,energy distribution,lipids composition

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Stress Biology 2022年第1期2卷 109-119页

作者： Qiang Meng Yueyao Li Yuxin Yuan shaowen wu Kan Shi Shuwen Liu College of Enology Northwest A&F UniversityYangling 712100ShaanxiChina Shaanxi Engineering Research Center for Viti-Viniculture Yangling 712100ShaanxiChina Viti-viniculture Engineering Technology Center of State Forestry and Grassland Administration Yangling 712100ShaanxiChina Heyang Experimental and Demonstrational Stations for Grape Northwest A&F UniversityHeyang 715300ShaanxiChina Ningxia Helan Mountain’s East Foothill Wine Experiment and Demonstration Station Northwest A&F UniversityYongning 750104NingxiaChina

This paper reported a wine-derived lactic acid bacterium,Lactiplantibacillus plantarum XJ25,which exhibited higher cell viability under acid stress upon methionine supplementation.Cellular morphology and the composition of the cytomembrane phospholipids revealed a more solid membrane architecture presented in the acid-stressed cells treated with methionine supplementation.Transcriptional analysis showed L.plantarum XJ25 reduced methionine transport and homocysteine biosynthesis under acid stress.Subsequent overexpression assays proved that methio-nine supplementation could alleviate the cell toxicity from homocysteine accumulation under acid stress.Finally,L.plantarum XJ25 employed energy allocation strategy to response environmental changes by balancing the uptake methionine and adjusting saturated fatty acids(SFAs)in membrane.These data support a novel mechanism of acid resistance involving methionine utilization and cellular energy distribution in LAB and provide crucial theoretical clues for the mechanisms of acid resistance in other bacteria.

关键词： Acid stress Lactiplantibacillus plantarum Methionine Cell viability Transcription level Membrane lipids

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电场条件下高盐溶液蒸发的分子动力学模拟

同济大学学报（自然科学版） 2024年第3期52卷 437-445页

作者：吴志根吴少文颜子涵齐孝圣同济大学环境与科学工程学院上海200092 上海污染控制与生态安全研究院上海200092 上海理工大学能源与动力工程学院上海200092

已有研究表明,外加电场能显著改变纯水蒸发过程和蒸发速率。为了探究外加电场对高盐溶液微观结构和蒸发性质的影响,采用分子动力学模拟方法对纯水和LiCl、KCl和CaCl23种盐溶液在平行液面(x方向)和垂直液面方向(z方向)上布置外加电场来... 详细信息

已有研究表明,外加电场能显著改变纯水蒸发过程和蒸发速率。为了探究外加电场对高盐溶液微观结构和蒸发性质的影响,采用分子动力学模拟方法对纯水和LiCl、KCl和CaCl23种盐溶液在平行液面(x方向)和垂直液面方向(z方向)上布置外加电场来观察其蒸发过程。模拟计算分析了电场对蒸发速率、水分子取向、氢键以及水合离子的影响,并且观察比较了电场下不同离子在溶液中的运动。结果显示,与纯水相反,平行液面的电场能显著提高盐溶液中自由水分子的占比,当平行液面电场强度从0变为0.3 v·nm-1时,LiCl、KCl和CaCl2溶液中用于评价蒸发的自由水分子占比分别增加了48%,38%和56%,高盐溶液蒸发速率得到有效提高。

关键词：高盐溶液溶液蒸发分子动力学模拟气液界面电场

USP11调节IGF2BP3介导口腔鳞状细胞癌细胞增殖和侵袭机制研究

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实用口腔医学杂志 2024年第3期40卷 377-384页

作者：郭红燕吴福焱王少文聊城市第四人民医院口腔科 252000

目的:探究去泛素化酶(USP11)通过调节胰岛素样生长因子2-mRNA结合蛋白3(IGF2BP3)表达影响口腔鳞状细胞癌(OSCC)恶性表型的分子机制。方法:免疫组化和Western blot检测OSCC患者(n=50)癌组织和癌旁组织中USP11蛋白表达,Western blot检测US... 详细信息

目的:探究去泛素化酶(USP11)通过调节胰岛素样生长因子2-mRNA结合蛋白3(IGF2BP3)表达影响口腔鳞状细胞癌(OSCC)恶性表型的分子机制。方法:免疫组化和Western blot检测OSCC患者(n=50)癌组织和癌旁组织中USP11蛋白表达,Western blot检测USP11蛋白在正常人口腔上皮角质细胞(HOK)和人OSCC细胞SCC-25和CAL-27中表达;Kaplan-Meier生存模型分析USP11高低表达对OSCC患者生存率的影响;siRNA USP11(si-USP11)转染SCC-25和CAL-27细胞敲低内源性USP11表达;CCK-8、划痕实验和Transwell实验检测细胞增殖、迁移和侵袭;Western blot检测敲低USP11后的IGF2BP3蛋白表达;敲低USP11和过表达IGF2BP3,检测OSCC细胞增殖、迁移和侵袭的变化;过表达USP11同时敲低IGF2BP3,检测敲低IGF2BP3对USP11过表达OSCC细胞增殖、迁移和侵袭的影响;裸鼠接种SCC-25细胞构建皮下移植瘤模型,考察敲低USP11对SCC-25细胞成瘤抑制作用。结果:与癌旁组织相比,OSCC患者癌组织中USP11蛋白免疫组化评分显著升高(P<0.01);与HOK细胞相比,SCC-25和CAL-27细胞USP11蛋白表达显著增加(P<0.01)。敲低USP11降低OSCC细胞增殖、迁移和侵袭能力(P<0.01),下调IGF2BP3蛋白表达。过表达IGF2BP3逆转USP11敲低对OSCC细胞增殖、迁移和侵袭能的抑制作用;敲低IGF2BP3逆转USP11过表达对OSCC细胞增殖、迁移和侵袭的促进作用。裸鼠致瘤性实验显示敲低USP11抑制移植瘤模型中SCC-25细胞体内生长。结论:USP11在OSCC患者癌组织中表达升高且高表达患者预后更差,USP11通过上调IGF2BP3蛋白表达促进OSCC细胞增殖、迁移和侵袭。

关键词： USP11 IGF2BP3 口腔鳞状细胞癌增殖

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典型南方丘陵山区地下水动态及影响因素分析

水文 2024年第2期44卷 66-73页

作者：方少文陈静吴智韦丽徐征文江西省水文监测中心江西南昌330002 河海大学水文水资源学院江苏南京210098 抚河水文水资源监测中心江西抚州344000

目前针对南方低山丘陵区地下水动态的研究较少,且仍存在对地下水位及其影响因素之间关系的空间非平稳性进行定量化表达方面的不足。以江西省抚河流域典型丘陵山区为例,分析丘陵山区地下水动态,并基于地理加权回归模型和多源数据,定量化... 详细信息

目前针对南方低山丘陵区地下水动态的研究较少,且仍存在对地下水位及其影响因素之间关系的空间非平稳性进行定量化表达方面的不足。以江西省抚河流域典型丘陵山区为例,分析丘陵山区地下水动态,并基于地理加权回归模型和多源数据,定量化表达影响因素在山地区和平原区对地下水位的贡献度排序,从时空尺度系统对比影响因素作用效果的差异。结果表明,研究区地下水动态以降水补给、侧向排泄为主,地下水位月际波动大,空间上呈现由南向北递减趋势。山地区和平原区地下水位均受降水量因子和高程因子影响最显著,在山地区地表水体面积占比因子对地下水位的贡献较大,而在平原区地表温度因子对地下水位的贡献较大。影响因素的作用效果均具有明显的空间异质性,随着降水量的增加,降水量因子对地下水位的影响逐渐增加,高程因子在山地区正效应明显,地表温度因子总体在平原区负效应明显。

关键词：地理加权回归丘陵山区地下水动态影响因素

基于纳米抗体-荧光素酶的黄曲霉毒素B1检测方法构建

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中国食品学报 2024年第4期24卷 349-360页

作者：陈文星王凤华谭晓亮晏石娟张军吴绍文甘肃农业大学资源与环境学院兰州730070 猪禽种业全国重点实验室广东省农作物种质资源保存和利用重点实验室广东省农业科学院农业生物基因研究中心广州510640

黄曲霉毒素B1(AFB1)是一种剧毒、致癌的食源性污染物,严重威胁食品安全和公共健康。为开发快速检测AFB1的生物发光酶联免疫分析(BLEIA)方法,系统测试了3种抗AFB1特异性纳米抗体(NB)与纳米荧光素酶(Nluc)融合蛋白(G8-Nluc、Nluc-NB26和Nl... 详细信息

黄曲霉毒素B1(AFB1)是一种剧毒、致癌的食源性污染物,严重威胁食品安全和公共健康。为开发快速检测AFB1的生物发光酶联免疫分析(BLEIA)方法,系统测试了3种抗AFB1特异性纳米抗体(NB)与纳米荧光素酶(Nluc)融合蛋白(G8-Nluc、Nluc-NB26和Nluc-NB28)的可溶性表达、纯化情况及酶催化活性。基于3种融合蛋白分别建立BLEIA检测体系,选择Nluc-NB26用于谷物样品的分析和验证。结果表明,Nluc-NB26的可溶性表达量最高,稳定性更好,Nluc-NB28的可溶性表达量次之,而G8-Nluc基本不可溶。不同表面活性剂对G8-Nluc的促溶解性研究表明,添加N-月桂酰肌氨酸钠可显著提高其溶解度,纯化得到的3种融合蛋白均具有良好的酶活性及抗原结合活性。基于融合蛋白的BLEIA检测结果显示,Nluc-NB28-BLEIA、G8-Nluc-BLEIA和Nluc-NB26-BLEIA体系检测AFB1的IC_(50)值分别为4.213,1.697,2.169 ng/mL,表明G8-Nluc-BLEIA体系的灵敏度最高,Nluc-NB26-BLEIA与前者接近,Nluc-NB28-BLEIA最低。综合考虑融合蛋白的可溶性表达量、稳定性及检测性能,对Nluc-NB26-BLEIA开展实际样品的分析和验证,结果表明:这一方法检测谷物中AFB1的平均回收率在91.1%~104.1%之间,与商业化酶联免疫吸附测定试剂盒结果相似,而检测的时间和试剂成本明显降低。研究结果为开发快速、高灵敏的AFB1检测技术提供参考。

关键词：黄曲霉毒素B1 纳米抗体纳米荧光素酶快速检测生物发光酶联免疫分析

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实用拜占庭容错共识算法的奖惩机制优化研究

计算机工程与应用 2024年第7期60卷 266-273页

作者：张苗李绍稳吴雨婷涂立静张磊杨尚雄安徽农业大学信息与计算机学院合肥230036 智慧农业技术与装备安徽省重点实验室合肥230036

联盟链是农业溯源行业的首选区块链方案,针对其核心共识机制实用拜占庭容错共识算法(PBFT)存在的共识安全性低、主节点出错概率大、通信开销大等问题,通过引入奖惩机制以及分组共识机制,提出了基于奖惩机制的改进实用拜占庭容错共识算... 详细信息

联盟链是农业溯源行业的首选区块链方案,针对其核心共识机制实用拜占庭容错共识算法(PBFT)存在的共识安全性低、主节点出错概率大、通信开销大等问题,通过引入奖惩机制以及分组共识机制,提出了基于奖惩机制的改进实用拜占庭容错共识算法。把节点划分为4类,制定节点信誉规则,增强节点主动性,减少拜占庭节点的参与;剔除作恶节点参与共识资格,统计其余节点信誉值及票数,动态筛选信誉良好节点作为共识节点,并依据最高信誉值规则选取主节点,从而提升共识安全、降低拜占庭节点当选主节点概率;以PBFT共识为基础,将共识过程简化为组内共识及全局共识两个阶段,并基于最少广播消息数提出最优分组,缓解共识节点数目增多导致通信量过大的问题。实验结果表明,该方案能够有效抑制拜占庭节点,提升共识安全性能,降低共识成本。

关键词：区块链共识算法奖惩机制分组共识