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Journal of Semiconductors 2023年第2期44卷 49-56页

作者： Xiaofei Liu Songyin Qiu Haiping Fang Lin Mei Hongli Jing Chunyan Feng shaoqiang wu Xiangmei Lin Institute of Animal Inspection and Quarantine Chinese Academy of Inspection and QuarantineBeijing 100176China Research Center of GACC for international Inspection and Quarantine Standards and Technical Regulations Beijing 100013China

Viral diseases represent one of the major threats for salmonids *** detection and identification of viral pathogens is the main prerequisite prior to undertaking effective prevention and control ***,sensitive,efficient and portable detection method is highly essential for fish viral diseases *** strategies are highly prevalent and fulfill the expanding demands of on-site detection with fast response,cost-effectiveness,high sensitivity,and *** the development of material science,the nucleic acid biosensors fabricated by semiconductor have shown great potential in rapid and early detection or screening for diseases at salmonids *** paper reviews the current detection development of salmonids viral *** present limitations and challenges of salmonids virus diseases surveillance and early detection are *** nucleic acid semiconductor biosensors are briefly *** perspective and potential application of biosensors in the on-site detection of salmonids diseases are discussed.

关键词： salmonids virus detection nucleic acid test biosensors semiconductor

Application and prospect of semiconductor biosensors in detection of viral zoonoses

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Journal of Semiconductors 2023年第2期44卷 40-48页

作者： Jiahao Zheng Chunyan Feng Songyin Qiu Ke Xu Caixia Wang Xiaofei Liu Jizhou Lv Haoyang Yu shaoqiang wu Institute of Animal Quarantine Chinese Academy of Inspection and QuarantineBeijing100176China Chinese Academy of Inspection and Quarantine Center for Biosafety Sanya 572024China National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China

The rapid spread of viral zoonoses can cause severe consequences,including huge economic loss,public health problems or even global crisis of *** detection technology plays a very important role in the prevention and control of such *** rapid and accurate detection of the pathogens of the diseases can directly lead to the early report and early successful control of the *** the advantages of being easy to use,fast,portable,multiplexing and cost-effective,semiconductor biosensors are kinds of detection devices that play an important role in preventing epidemics,and thus have become one of the research ***,we summarized the advances of semiconductor biosensors in viral zoonoses *** discussing the major principles and applications of each method for different pathogens,this review proposed the directions of designing semiconductor biosensors for clinical application and put forward perspectives in diagnostic of viral zoonoses.

关键词： semiconductor biosensor viral zoonoses graphene silicon nanowire carbon nanotube

Molecular Identification of D. nuttalli and ***

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Animal Husbandry and Feed Science 2014年第4期6卷 159-164,215页

作者： Lv Jizhou Wang Zhenbao Yuan Xiangfen wu shaoqiang Institute of Animal Quarantine Chinese Academy of Inspection and Quarantine Yili Entry-exit Inspection and Quarantine Bureau

This study was to establish a molecular identification method to distinguish Dermacentor nuttalli(D. nuttalli) and Dermacentor marginatus(D. marginatus),and to study their phylogenetic relationship. The ticks were collected from domestic animals in Xinjiang Uygur Autonomous Region for morphological identification,then their genomic DNAs were isolated for amplifying mitochondrial 16 S rRNA and cytochrome oxidase subunit I gene(COI). The phylogenetic tree was constructed based on the sequencing results of PCR products by employing Mega 5. 0 and Mrbayes 3. 2 for homology analysis. Clustering analysis PCR product for 16 S rRNA from both D. marginatus and D. nuttalli was clustered together with their respective 16 S rRNA sequences previously accessed in GenBank,and that for COI gene as well. These are basically identical with morphological identification results. Our results indicate that morphological identification,combined with molecular markers,would be a simple and accurate method for distinguishing D. nuttalli and D. marginatus.

关键词： Mitochondrial cytochrome oxidase(COI) 16S rRNA Dermacentor nuttalli Dermacentor marginatus Molecular identification

In vitro Culture of Bonamia ostreae and Its Identification

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Animal Husbandry and Feed Science 2014年第4期6卷 206-208页

作者： Feng Chunyan Wang Fei Yang Lianru wu shaoqiang Institute of Animal Quarantine Chinese Academy of Inspection and Quarantine College of Animal Science and Veterinary Medicine Inner Mongolia Agricultural University

In recent years,bonamiosis has frequently occurred in European areas,which has caused the death of oyster in a wide range and brought enormous economic losses to the breeding industry of oyster. Nowadays,the study on Bonamia sp. is still in the elementary phase. The technology of pathogen’s culture in vitro is the basis for further study on the pathogenesis of Bonamia sp.,its interaction with hosts and the prevention and control of the related disease. In this study,total tissues of oyster identified by PCR were used as culture media to in vitro culture. After one month,they were identified by the method of in situ hybridization. It was found that the results of in situ hybridization were accordant with PCR results. And Bonamia ostreae were detected by in situ hybridization after B. ostreae were cultured for one month. We successfully established a simple and feasible method for in vitro culturing B. ostreae.

关键词： Bonamia ostreae Culture in vitro Identification In situ hybridization

白藜芦醇通过PROX1/SIRT1信号通路调控山羊肌纤维类型转化的机制研究

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畜牧兽医学报 2025年第1期56卷 201-212页

作者：吴少强刘雨帆韦义荣黄艳娜蒋钦杨广西大学动物科学技术学院广西畜禽繁育与疾病防控重点实验室南宁530004

旨在解析白藜芦醇(resveratrol, RES)介导PROX1基因调控山羊骨骼肌肌纤维类型转化的分子机制。体内试验部分,试验选取12只180日龄去势努比亚山羊((28.25±0.26)kg)随机分为2组,每组6个重复。以补饲0、150 mg·kg^(-1) RES组山羊背最长... 详细信息

旨在解析白藜芦醇(resveratrol, RES)介导PROX1基因调控山羊骨骼肌肌纤维类型转化的分子机制。体内试验部分,试验选取12只180日龄去势努比亚山羊((28.25±0.26)kg)随机分为2组,每组6个重复。以补饲0、150 mg·kg^(-1) RES组山羊背最长肌组织为试验样品,采用实时荧光定量PCR(RT-qPCR)及Western blot技术检测PROX1基因的表达量;体外试验,基于课题组前期试验所筛选出的20μmol·L^(-1) RES是细胞培养最佳浓度,通过转染PROX1干扰片段及干扰片段与20μmol·L^(-1) RES共处理山羊骨骼肌成肌细胞,检测CaN/NFAT通路关键基因与肌纤维四型标志基因在mRNA水平及蛋白水平的表达情况;同时,利用蛋白-蛋白对接技术检测SIRT1与PROX1蛋白是否互作,并通过SIRT1抑制剂EX-527和RES共处理山羊成肌细胞,验证SIRT1与CaN/NFAT通路的调控关系。结果表明,与对照组相比,补饲150 mg·kg^(-1) RES处理山羊成肌细胞可使SIRT1基因及CaN/NFAT通路关键基因的mRNA表达量极显著上调(P<0.001),使MYH7蛋白的表达水平显著上调(P<0.01),MYH4蛋白的表达水平显著下降(P<0.01)。而干扰PROX1基因后,CaN/NFAT通路关键基因mRNA表达水平及MYH7、MYH4基因mRNA表达水平与蛋白表达水平的表达情况都与上述相反,该情况则在干扰片段与RES共处理成肌细胞后发生改变。蛋白-蛋白对接结果表明,SIRT1蛋白与PROX1蛋白可以互作。SIRT1抑制剂处理成肌细胞后,CaN/NFAT通路关键基因(NFATc1、TNNC1、TNNI1、MYOZ2、SLN)的mRNA表达量较CON组显著下调(P<0.01),再经过RES干预后,TNNC1、TNNI1的mRNA表达量极显著上调(P<0.01)。本研究初步揭示了RES通过SIRT1/PROX1/CaN/NFAT途径调控肌纤维类型转化的机制,为后续畜禽肉品质的改良提供理论依据。

关键词：白藜芦醇(RES) CaN/NFAT信号通路山羊成肌细胞肌纤维转化

Identification of tick-borne pathogens using metagenomic analyses in *** feeding on humans in downtown Beijing

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Animal Diseases 2021年第3期1卷 173-181页

作者： Jizhou Lv Huiyu Wang Xueqing Han Lin Mei Xiangfen Yuan Yufang Kong Junhua Deng Zhen F.Fu shaoqiang wu Xiangmei Lin Institute of Animal Inspection and Quarantine Chinese Academy of Inspection and QuarantineBeijing 100176China Department of Pathology College of Veterinary MedicineUniversity of GeorgiaAthens 30602USA

On August 14th,2018,a Beijing resident living in Xicheng District found a female *** tick attached to the skin at the front of his upper *** examination,the patient was afebrile and appeared *** species of the tick was identified through morphological characteristics and phylogenetic analysis based on cytochrome C oxidase subunit *** *** tick was screened for tick-borne pathogens such as viruses,bacteria and *** pathogens were screened by PCR and sequencing,while DNA pathogens were screened by metagenomic *** was found that the tick was positive for the DNA sequences of zoonotic and animal pathogens such as A phagocytophilum,Ehrlichia minasensis and *** the good health condition of the patient,we hypothesized that the pathogens originated from the tick specimen itself rather than host blood *** the first time,our study reveals the possible risk of transmission of tick-borne pathogens to human beings through tick bit in downtown *** research is needed to screen for tick-borne pathogens among unfed ticks collected from central Beijing.

关键词： Tick-borne pathogens Metagenomic analyses H.longicornis Downtown Beijing

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聚合物波导光栅耦合器的衍射场仿真（英文）

红外与激光工程 2019年第4期48卷 279-284页

作者：吴少强冯向华卫正统吴天昊中国人民解放军信息工程大学基础部河南郑州450000

为了实现横截面尺寸为50μm×50μm的聚硅氧烷聚合物光波导的耦合转向问题,设计了一种表面覆盖高折射率包层的多层蚀刻光栅耦合器。首先,分析了影响聚合物波导光栅耦合器耦合效率的结构因素;然后,采用在光栅表面蚀刻高折射率层的方法,... 详细信息

为了实现横截面尺寸为50μm×50μm的聚硅氧烷聚合物光波导的耦合转向问题,设计了一种表面覆盖高折射率包层的多层蚀刻光栅耦合器。首先,分析了影响聚合物波导光栅耦合器耦合效率的结构因素;然后,采用在光栅表面蚀刻高折射率层的方法,提高了聚合物波导光栅耦合器的耦合效率;接着,对不同的周期(范围:100～4 000 nm)和不同的蚀刻深度(范围:0～50 000 nm)进行排列组合,形成不同的光栅结构,基于时域有限差分法编写程序,遍历所有情况,得到不同光栅结构下的光场情况以及其耦合效率,找到使耦合效率最大的周期以及蚀刻深度。最后,设计了多层蚀刻的光栅耦合器,进一步提高耦合效率。当蚀刻深度为5 000 nm,光栅周期为2 600 nm时,带高折射率层的聚硅氧烷聚合物光波导均匀光栅耦合器的耦合效率达到最大,为17.2%。采用多层蚀刻的方式,对结构进行优化,其耦合效率能达到37.4%。为聚硅氧烷聚合物光波导在光互连中的实际应用提供了理论依据。

关键词：光互连光栅耦合器时域有限差分法聚硅氧烷聚合物光波导耦合效率

Prevalence and Countermeasures of Porcine Circovirus 3

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Animal Husbandry and Feed Science 2018年第3期10卷 185-188页

作者： Feng Chunyan Wang Caixia Liu Dandan Zhang Yongning Du Fangyuan Lin Xiangmei wu shaoqiang Animal Quarantine Institute Chinese Academy of Inspection and Quarantine

The development history, etiology, epidemiology, clinical symptoms, pathological changes and diagnosis technology of porcine circovirus 3 were summarized, and countermeasures for prevention and control of the disease ... 详细信息

关键词： Porcine circovirus 3 Etiology Epidemiology Diagnosis Prevention and control

Antigen-capture ELISA for grass carp reovirus 873

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Antigen-capture ELISA for grass carp reovirus 873

中国水产学会鱼病专业委员会2013年学术研讨会

作者： Jing Hongli Gao long ying Zhang Min Wang Na lin xiangmei wu shaoqiang Research Centre of Aquatic Animal Diseases the Institute of Animal QuarantineChinese Academy of Inspection and Quarantine Shen zhen Entry-exit Inspection and Quarantine Bureau

An antigen-capture ELISA for grass carp reovirus 873(GCRV 873)was *** employs polyclonal antiserum directed against grass carp reovirus *** antigen boundby the capture mAb(16B7)was detected by addition of the goat anti-mouse Ig peroxidaseconjugation,followed by addition of the enzyme ***-873 was detected in culturesupernatants(6.25×105pfu/ml)and in ectracts of kidney and spleen of infected grass *** was highly specific:Viral hemorrhagic septicemia virus,Infectious haematopoietic necrosisvirus,spring viraemia of carp virus,Epizootic haematopoietic necrosis virus,Chinook salmonreovirus could not be detected by the antigen-capture ELISA for GCRV 873.

关键词： Grass carp reovirus(GCRV) fish viral diseases Antigen-capture ELISA

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应对战场电磁干扰的自适应动态指标配置方法

兵工学报 2019年第8期40卷 1648-1657页

作者：吴天昊王劲松牛侃孙淼吴少强战略支援部队信息工程大学河南郑州450001 32553部队海南海口570100 31007部队北京100079

在敌我双方对抗中,持续性电磁冲突和干扰可能导致用频装备效能下降甚至瘫痪,如何在电磁干扰环境下实现快速响应并精确配置元器件参数,已成为打赢现代化战争的关键能力之一。针对战场电磁干扰具有不确定、动态、多维等特点,在指标属性权... 详细信息

在敌我双方对抗中,持续性电磁冲突和干扰可能导致用频装备效能下降甚至瘫痪,如何在电磁干扰环境下实现快速响应并精确配置元器件参数,已成为打赢现代化战争的关键能力之一。针对战场电磁干扰具有不确定、动态、多维等特点,在指标属性权重和时序权重未知的决策环境下,提出一种基于灰色靶心决策理论的自适应动态指标配置方法。以靶心度为衡量标准,纵向给出多时序区间的自适应灰靶决策模型,横向给出采集区间时序的自适应调整方法,以实现对策指标自适应调整和确定。结合战场电磁干扰类型及原理,构建了自适应动态指标配置模型。仿真实验结果表明,与标准算法对比,该改进算法具有可行性与精确性,可为决策者科学合理配置装备指标参数提供参考。

关键词：电磁干扰网络战场自适应灰靶决策动态指标指标确定频谱管控