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Establishment of a dmp based maternal haploid induction system for polyploid Brassica napus and Nicotiana tabacum

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Journal of Integrative Plant Biology 2022年第6期64卷 1281-1294页

作者： Yu Zhong Yuwen Wang Baojian chen Jinchu Liu Dong Wang Mengran Li Xiaolong Qi chenxu Liu Kim Boutilier shaojiang chen National Maize Improvement Center of China Key Laboratory of Crop Heterosis and Utilization/Engineering Research Center for Maize BreedingMinistry of Educati onCollege of Agronomy and Biotech no logyChina Agricultural Un iversityBeijing 100193China Bioscienee Wageningen University and Research6700 AAWageningenThe Netherlands

Doubled haploid(DH) technology is used to obtain homozygous lines in a single generation, a technique that significantly accelerates the crop breeding trajectory. Traditionally, in vitro culture is used to generate DHs, but this technique is limited by species and genotype recalcitrance. In vivo haploid induction(HI) through seed is widely and efficiently used in maize and was recently extended to several other crops. Here we show that in vivo HI can be triggered by mutation of DMP maternal haploid inducer genes in allopolyploid(allotetraploid) Brassica napus and Nicotiana tabacum. We developed a pipeline for selection of DMP orthologs for clustered regularly interspaced palindromic repeats mutagenesis and demonstrated average amphihaploid induction rates of2.4% and 1.2% in multiple B. napus and N. tabacum genotypes, respectively. These results further confirmed the HI ability of DMP gene in polyploid dicot crops. The DMP-HI system offers a novel DH technology to facilitate breeding in these crops. The success of this approach and the conservation of DMP genes in dicots suggest the broad applicability of this technique in other dicot crops.

关键词： amphihaploid Brassica napus dicot crops doubled haploid tech no logy maternal haploid in ducti on Nicotiana tabacum polyploid

Development of high-oil maize haploid inducer with a novel phenotyping strategy

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The Crop Journal 2022年第2期10卷 524-531页

作者： chenxu Liu Jinlong Li Ming chen Wei Li Yu Zhong Xin Dong Xiaowei Xu chen chen Xiaolong Tian shaojiang chen College of Agronomy and Biotechnology Beijing Key Laboratory of Crop Genetic ImprovementLaboratory of Crop Heterosis and UtilizationMinistry of EducationNationalMaize Improvement Center of ChinaChina Agricultural UniversityBeijing 100193China Chongqing Academy of Agricultural Sciences Chongqing 401329China

Doubled haploid(DH) technology is important in modern maize breeding. Haploid inducers determine the efficiency of both haploid induction and identification. It has taken decades to improve the efficiency,haploid induction rate(HIR), from the ~2% of the ancestor haploid inducer, stock6, to the ~10% of modern haploid inducers. Improvement of kernel oil content(KOC) would further enhance haploid identification efficiency. Using molecular marker-assisted selection, in combine with the number of haploids per ear as phenotypic criterion, we developed a new high-oil haploid inducer line, CHOI4, with a mean HIR of 15.8%and mean KOC of 11%. High KOC of CHOI4 can achieve a mean accuracy greater than 90% in identification of haploids of different backgrounds, with reduced false discovery rates and false negative rates in comparison with the previous high-oil haploid inducer line, CHOI3. Comparison of phenotypic selection strategies suggested that the number of haploids per ear can be used as a phenotyping criterion during haploid inducer line development. CHOI4 could further increase the efficiency of large-scale DH breeding programs with lower cost.

关键词： Doubled haploid Marker-assisted selection Kernel oil content Haploid identification Haploids per ear

Construction of homozygous diploid potato through maternal haploid induction

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aBIOTECH 2022年第3期3卷 163-168页

作者： Jinzhe Zhang Jian Yin Jiayi Luo Die Tang Xijian Zhu Jie Wang Zhihong Liu Pei Wang Yu Zhong chenxu Liu Canhui Li shaojiang chen Sanwen Huang Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of AgricultureSino-Dutch Joint Laboratory of Horticultural GenomicsBeijing100081China Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen518120China The AGISCAAS-YNNU Joint Academy of Potato Sciences Yunnan Normal UniversityKunming650500China National Maize Improvement Center of China Key Laboratory of Crop Heterosis and Utilization/Engineering Research Center for Maize BreedingMinistry of EducationCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing100193China

Reinventing the tetraploid potato into a seed-propagated,diploid,hybrid potato would significantly accelerate potato breeding.In this regard,the development of highly homozygous inbred lines is a prerequisite for breeding hybrid potatoes,but self-incompatibility and inbreeding depression present challenges for developing pure inbred lines.To resolve this impediment,we developed a doubled haploid(DH)technology,based on mutagenesis of the potato DOMAIN OF UNKNOWN FUNCTION 679 membrane protein(StDMP)gene.Here,we show that a deficiency in StDMP allows the generation of maternal haploids for generating diploid potato lines.An exercisable protocol,involving hybridization,fluorescent marker screening,molecular and flow cytometric identification,and doubling with colchicine generates nearly 100%homozygous diploid potato lines.This dmp-triggered haploid induction(HI)system greatly shortens the breeding process and offers a robust method for generating diploid potato inbred lines with high purity.

关键词： Doubled haploid technology Haploid induction Diploid potato Inbred lines StDMP

Integration of genomic selection with doubled-haploid evaluation in hybrid breeding: From GS 1.0 to GS 4.0 and beyond

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Molecular Plant 2022年第4期15卷 577-580页

作者： Junjie Fu Yangfan Hao Huihui Li Jochen C.Reif shaojiang chen Changling Huang Guoying Wang Xinhai Li Yunbi Xu Liang Li Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China Leibniz Institute of Plant Genetics and Crop Plant Research(IPK) 06466 Stadt SeelandGermany College of Agronomy and Biotechnology China Agricultural UniversityBeijing 100193China International Maize and Wheat Improvement Center(CIMMYT) El BatanTexcoco 56130Mexico

DOUBLED-HAPLOID TECHNOLOGY FACES A GREAT CHALLENGE FOR HYBRID BREEDING,Ensuring food security for the ever-growing population is a common mission and a great challenge for agricultural scientists worldwide.Historically,advances in crop breeding and management practices have contributed substantially to crop productivity.Indeed,the substantial increase in global grain yields over the last eight decades is largely due to the adoption of hybrids.However,the rate of increase of hybrid yields began to slow down in the early 2000s,and since then,it has reached a plateau for many crops and regions(https://***).Therefore,we must find solutions to accelerating genetic gain and boost hybrid development,for which developing new breeding technologies provides novel creative opportunities.

关键词： breeding crops doubled

High-throughput haploid induction in species with bisexual flowers

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Plant Communications 2023年第1期4卷 209-212页

作者： Xiaolong Qi Jinchu Liu Zongkai Liu chen chen Baojian chen Shuwei Guo Zhongfu Ni Yu Zhong shaojiang chen chenxu Liu College of Agronomy and Biotechnology China Agricultural UniversityBeijing 100193China National Maize Improvement Center China Agricultural UniversityBeijing 100193China Hainan Yazhou Bay Seed Laboratory Sanya 572024China

Dear Editor,Doubled haploid(DH)technology can significantly accelerate the development of homozygous lines.DH breeding has achieved great success inmaize because of the discovery of the first haploid inducer,Stock6,and the development of a series of high-efficiency haploid inducers(Hu et al.,2016).Pioneering studies on the genetic basis of haploid induction(HI)revealed that loss-offunction mutation of the phospholipase gene ZmPLA1/MATL/NLD triggers HI and that the HI rate(HIR)can be dramatically enhanced by a single nucleotide substitution from T to C in ZmDMP(Jacquier et al.,2020).Remarkably,knockout of ZmPLA1/MATL/NLD homologs in rice,wheat,and foxtail millet results in HIRs of 2%–6%,5%–15%,and 2%–3%,respectively(Jacquier et al.,2020;cheng et al.,2021).In addition,loss of function of ZmDMP-like genes enables HI in species including Arabidopsis,tomato,rapeseed,tobacco,etc.,with an average HIR of around 2%(Zhong et al.,2020,2022a,2022b).These successes have laid solid foundations for the construction of a universal DH breeding system in different crop species.More importantly,HI-Edit/IMGE systems that enable gene editing in elite germplasms have been established on the basis of HI,making HI even more important(Kelliher et al.,2019;Wang et al.,2019).

关键词： breeding elite enable

Establishment of an efficient haploid identification system by engineering anthocyanin accumulation in the wheat embryo

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Plant Communications 2023年第3期4卷 7-10页

作者： Xiaolong Qi Shuwei Guo Yu Zhong Baojian chen Zongkai Liu Tongzheng Yan Bin Feng Zhongfu Ni shaojiang chen chenxu Liu College of Agronomy and Biotechnology China Agricultural UniversityBeijing 100193China National Maize Improvement Center China Agricultural UniversityBeijing 100193China Sanya Institute of China Agricultural University Sanya 572024China Hainan Yazhou Bay Seed Laboratory Sanya 572024China These authors contributed equally to this article

Dear Editor,Bread wheat(Triticum aestivum)is one of the most important food crops and provides approximately 20%of the food calories for human consumption.A 70%increase in wheat production is needed by 2050 to keep pace with the growing global population(International Wheat Genome Sequencing,2014).Developing superior cultivars is an efficient way to improve yield.Nevertheless,conventional breeding is time consuming,as more than eight generations are needed to develop new plant varieties.Using doubled haploid(DH)technology,homozygous lines can be produced in only two generations,dramatically accelerating the breeding process.In wheat,haploids can be obtained by cross pollination with corn pollen followed by embryo rescue(Laurie and Bennett,1988).In maize,haploids can be induced by haploid inducer lines derived from Stock6(Liu et al.,2022).The cloning of two genes that control haploid induction(HI)in maize,MATL/ZmPLA1/NLD and ZmDMP,paved the way for DH breeding in more crop species(Jacquier et al.,2020).Further studies have shown that loss of function of TaPLAs triggers wheat HI with an efficiency of 5.88%to 31.6%(Liu et al.,2020a,2020b);this would be a promising approach for establishing a new,simple,and more efficient DH breeding method in wheat.

关键词： breeding crops cultivar

Genome Editing and Double-Fluorescence Proteins Enable Robust Maternal Haploid Induction and Identification in Maize

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Molecular Plant 2018年第9期11卷 1214-1217页

作者： Le Dong Lina Li Changlin Liu chenxu Liu Shuaifeng Geng Xinhai Li Changling Huang Long Mao shaojiang chen Chuanxiao Xie Institute of Crop Science Chinese Academy of Agricultural Sciences National Key Facility for Crop Gene Resources and Genetic Improvement Beijing 100081 China National Maize Improvement Center of China China Agricultural University No. 2 Yuan Ming Yuan West Road Haidian District Beijing 100193 China

Dear Editor Genome editing technologies have paved the way for exciting and novel applications in plant biotechnology. Doubled haploid （DH） technology has a significant and valuable advantage over traditional approaches in crop breeding. Unlike traditional breeding processes, which may take over eight generations to stabilize the genetic background of interest.

关键词： PHOSPHOLIPASE MARKER

Genome-wide prediction in a hybrid maize population adapted to Northwest China

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The Crop Journal 2020年第5期8卷 830-842页

作者： Guoliang Li Yuan Dong Yusheng Zhao Xiaokang Tian Tobias Würschum Jiquan Xue shaojiang chen Jochen C.Reif Shutu Xu Wenxin Liu National Maize Improvement Center of China Key Laboratory of Crop Heterosis and Utilization(MOE)China Agricultural UniversityBeijing 100193China Key Laboratory of Biology and Genetic Improvement of Maize in Arid Area of Northwest Region(Ministry of Agriculture) College of AgronomyNorthwest A&F UniversityYangling 712100ShaanxiChina Department of Breeding Research Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)06466 Stadt SeelandGermany Institute of Plant Breeding Seed Science and Population GeneticsUniversity of Hohenheim70599 StuttgartGermany

Genome-wide prediction is a promising approach to boost selection gain in hybrid breeding.Our main objective was to evaluate the potential and limits of genome-wide prediction to identify superior hybrid combinations adapted to Northwest China.A total of 490 hybrids derived from crosses among 119 inbred lines from the Shaan A and Shaan B heterotic pattern were used for genome-wide prediction of ten agronomic traits.We tested eight different statistical prediction models considering additive(A)effects and in addition evaluated the impact of dominance(D)and epistasis(E)on the prediction ability.Employing five-fold cross validation,we show that the average prediction ability ranged from 0.386 to 0.794 across traits and models.Six parametric methods,i.***.ridge regression,LASSO,Elastic Net,Bayes B,Bayes C and reproducing kernel Hilbert space(RKHS)approach,displayed a very similar prediction ability for each trait and two non-parametric methods(random forest and support vector machine)had a higher prediction performance for the trait rind penetrometer resistance of the third internode above ground(RPR_TIAG).The models of A+D RKHS and A+D+E RKHS were slightly better for predicting traits with a relatively high non-additive variance.Integrating trait-specific markers into the A+D RKHS model improved the prediction ability of grain yield by 3%,from 0.528 to 0.558.Of all 6328 potential hybrids,selection of the top 44 hybrids would lead to a 6%increase in grain yield compared with Zhengdan 958,a commercially successful hybrid variety.In conclusion,our results substantiate the value of genome-wide prediction for hybrid breeding and suggest dozens of promising single crosses for developing high-yielding hybrids for Northwest China.

关键词： breeding prediction forest

A 4-bp Insertion at ZmPLA 1 Encoding a Putative Phospholipase A Generates Haploid Induction in Maize

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Molecular Plant 2017年第3期10卷 520-522页

作者： chenxu Liu Xiang Li Dexuan Meng Yu Zhong chen chen Xin Dong Xiaowei Xu Baojian chen Wei Li Liang Li Xiaolong Tian Haiming Zhao Weibin Song Haishan Luo Qinghua Zhang Jinsheng Lai Weiwei Jin Jianbing Yan shaojiang chen National Maize Improvement Center of China Key Laboratory of Crop Heterosis and Utilization (MOE) China Agricultural University No. 2 Yuan Ming Yuan West Road Haidian District Beijing 100193 China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 China Maize Research Institute Chongqing Academy of Agricultural Sciences Baishiyi Town Jiulongpo District Chongqing 401329 China These aut-hors contributed equally to this article.

Maize is one of the most important crops in the world and is also an essential raw material for the food, fuel, and fodder industries. Maize hybrids are widely used today, and gain of elite inbred lines is a crucial s... 详细信息

关键词： A 4-bp Insertion ZmPLA1 Encoding Putative Phospholipase