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Transportation of AIE-visualized nanoliposomes is dominated by the protein corona

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National Science Review 2021年第6期8卷 23-33页

作者： Yi-Feng Wang chunqiu Zhang Keni Yang Yufei Wang shaobo Shan Yan Yan Kenneth A.Dawson chen Wang Xing-Jie Liang cAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Center for Excellence in NanoscienceNational Center for Nanoscience and Technology of China University of chinese Academy of Sciences State Key Laboratory of Medicinal chemical Biology Key Laboratory of Bioactive Materials of Ministry of Educationand College of Life SciencesNankai University centre for Bio Nano Interactions School of ChemistryUniversity College Dublin School of Biomolecular and Biomedical Science University College Dublin Guangdong Provincial Education Department Key Laboratory of Nano-Immuno-regulation Tumor Microenvironment The Second Affiliated HospitalGuangzhou Medical University

Liposomes, especially cationic liposomes, are the most common and well-investigated nanocarriers for biomedical applications, such as drug and gene delivery. Like other types of nanomaterials, once liposomes are incubated in a biological milieu, their surface can be immediately cloaked by biological components to form a protein corona, which confers a new ‘biological identity’ and modulates downstream interactions with cells. However, it remains unclear how the protein corona affects the transportation mechanism after liposomes interact with cells. Here, we employed home-made aggregation-induced-emission-visualized nanoliposomes TR4@Lipo as a model to investigate transportation with or without the protein corona by optical imaging techniques. The results show that the protein corona can change the cellular transportation mechanism of TR4@Lipo from energy-independent membrane fusion to energy-dependent *** protein corona also modulates the intracellular distribution of loaded cargoes. This knowledge furthers our understanding of bio-nano interactions and is important for the efficient use of cationic liposomes.

关键词： aggregation-induced emission (AIE) cationic liposomes protein corona endocytosis membrane fusion

Transcriptome Analysis of crassostrea sikamea (♀) × crassostrea gigas (♂) Hybrids Under and After Thermal Stress

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Journal of Ocean University of china 2022年第1期21卷 213-224页

作者： ZHANG Xuekai FAN chao ZHANG Xingzhi LI Qiongzhen LI Yangchun MA Peizhen YUE shaobo HUO Zhongming WANG Zhaoping Fisheries college Ocean University of ChinaQingdao 266003China Guangxi Academy of Fisheries Sciences Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy AquacultureNanning 530021China

crossbreeding is an effective approach to manage the genetic decline in ***-way hybrids of crassostrea sikamea(♀)and crassostrea gigas(♂)have advantages in growth traits and adaptation to high ***,we used high-throughput sequencing to analyze the molecular processes in the hybrids under and after thermal *** hybrids were cultured in the seawater with an increasing temperature from 25℃to 40℃during 10 hours,which is regarded as the thermal stress *** the temperature decreased from 40℃to 25℃within 2 h,which is regarded as the recovery *** this study,1293 significant diffe-rentially expressed genes(DEGs)were obtained under thermal stress,of which 576 were upregulated and 717 were downregulated,and 740 significant differentially expressed genes(DEGs)were obtained in the recovery stage,of which the number of upregulated and downregulated genes was 409 and 331,*** antigen processing and presentation,NOD-like,and NF-kappa B path-ways were significantly enriched during the thermal stress *** MAPK and PPAR signaling pathways were significantly enrich-ed during the recovery *** HSP70,HSP90,and cANX genes were strongly and rapidly upregulated in the control/thermal stress groups but were slightly less upregulated in the thermal stress/recovery *** results indicate that the innate immune system or nonspecific immunity was deployed to protect interior tissues from thermal *** addition,85%of the mutual DEGs were involved in bidirectional regulation(up/down or down/up)when the oysters were removed from the thermal stress to *** study provides preliminary insight into the molecular response of ***(♀)and ***(♂)hybrids to thermal stress and provides a basis for future studies on temperature-adaptation and the possible expansion of hybrid breeding.

关键词： oyster hybrids gill thermal stress recovery transcriptome

Flexible triboelectric nanogenerator toward ultrahigh-frequency vibration sensing

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Nano Research 2022年第8期15卷 7484-7491页

作者： Zhiwei Lin chenchen Sun Gaoqiang Zhang Endong Fan Zhihao Zhou Ziying Shen Jun Yang Mingyang Liu Yushu Xia shaobo Si Jin Yang Key Laboratory of Optoelectronic Technology&Systems(Ministry of Education) Department of Optoelectronic EngineeringChongqing UniversityChongqing 400044China chongqing Institute of Green and Intelligent Technology Chinese Academy of SciencesChongqing 400714China

Flexible high-frequency vibration sensors are highly desirable in various real-world applications such as structural health monitoring,environmental monitoring,and the internet of ***,developing a facile and effective method to fabricate vibration sensors simultaneously featuring high vibration frequency response-ability and flexibility remains a grand ***,we report a flexible ultrahigh-frequency triboelectric vibration sensor(UTVS)prepared by a layer-particle-layer *** to the flexibility of the materials(i.e.,polyethylene terephthalate membrane)and the ultrahigh-frequency vibration response-ability of internal microparticles,the flexible UTVS exhibits an enhanced working frequency range of 3–170 kHz,which is much broader than previously reported triboelectric vibration ***,the UTVS can work not only in a flat state but also in a bent state due to its flexibility and the unique layer-particle-layer structural *** UTVS shows nanometer-level vibration response-ability,omnidirectional response,stability in the temperature range of 10–70°c,good frequency resolution of 0.01 kHz,and excellent performance in burst vibration detection(e.g.,pencil lead break events and impact events from falling steel balls).With a collection of compelling features,the device is successfully demonstrated in vibration monitoring of curved structures(e.g.,real-time water pipeline leak monitoring).Such a flexible ultrahigh-frequency triboelectric vibration sensor holds great potential in a wide range of practical applications,such as communication,health care,and infrastructure monitoring.

关键词： vibration sensing structural health monitoring flexible electronics self-powered sensor

Exploring the Spatial control of Topotactic Phase Transitions Using Vertically Oriented Epitaxial Interfaces

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Nano-Micro Letters 2022年第1期14卷 47-58页

作者： Wenrui Zhang Jie Zhang shaobo c.eng christopher M.Rouleau Kim Kisslinger Lihua Zhang Yimei Zhu Thomas Z.Ward Gyula Eres Materials Science and Technology Division Oak Ridge National LaboratoryOak RidgeTN 37831USA Ningbo Institute of Materials Technology and engineering Chinese Academy of SciencesNingboZhejiang 315201P.R.China condensed Matter Physics and Materials Science Brookhaven National LaboratoryUptonNY 11973USA center for Nanophase Materials Sciences Oak Ridge National LaboratoryOak RidgeTN 37831USA center for Functional Nanomaterials Brookhaven National LaboratoryUptonNY 11973USA

engineering oxygen vacancy formation and distribution is a powerful route for controlling the oxygen sublattice evolution that affects diverse functional *** controlling of the oxygen vacancy formation process is particularly important for inducing topotactic phase transitions that occur by transformation of the oxygen *** we demonstrate an epitaxial nanocomposite approach for exploring the spatial control of topotactic phase transition from a pristine perovskite phase to an oxygen vacancy-ordered brownmillerite(BM)phase in a model oxide La_(0.7)Sr_(0.3)MnO_(3)(LSMO).Incorporating a minority phase NiO in LSMO films creates ultrahigh density of vertically aligned epitaxial interfaces that strongly influence the oxygen vacancy formation and distribution in *** structural characterizations reveal strong interactions between NiO and LSMO across the epitaxial interfaces leading to a topotactic phase transition in LSMO accompanied by significant morphology evolution in *** the NiO nominal ratio as a single control parameter,we obtain intermediate topotactic nanostructures with distinct distribution of the transformed LSMO-BM phase,which enables systematic tuning of magnetic and electrical transport *** use of self-assembled heterostructure interfaces by the epitaxial nanocomposite platform enables more versatile design of topotactic phase structures and correlated functionalities that are sensitive to oxygen vacancies.

关键词： Epitaxial interface Nanocomposite Functional oxides Oxygen vacancy Topotactic phase transition

免疫组化MYB和Notch1在乳腺腺样囊性癌中的应用

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临床与实验病理学杂志 2024年第9期40卷 942-947页

作者：张晓红张晓丽韦志永王晓映眭怡群刘晓江王志蕙姚少波薛梅临沂市肿瘤医院病理科临沂276000 临沂市人民医院病理科临沂276000 山东大学第二医院病理科济南250012 苏州大学附属第二医院病理科苏州215004

目的探讨MYB、Notch1免疫组化染色在经典型乳腺腺样囊性癌(classic adenoid cystic carcinoma of the breast, c-Adcc)、具有基底细胞样特征的实体型乳腺腺样囊性癌(solid-basaloid adenoid cystic carcinoma of the breast, SB-Adcc)鉴别诊断中的应用价值。方法收集病理科存档的20例c-Adcc、6例SB-Adcc及65例其他乳腺病变组织,分别行MYB、Notch1免疫组化染色,并对26例Adcc行FISH检测,6例SB-Adcc行NGS检测。结果 (1)MYB免疫组化染色显示:c-Adcc(20/20)弥漫中等或强阳性,SB-Adcc(4/6)弥漫中等或强阳性;胶原小体病(5/5)局灶或弥漫弱阳性;恶性腺肌上皮瘤(3/3)局灶中等或强阳性;8例产基质的癌、9例分泌性癌及40例非特殊型三阴型乳腺癌均阴性。(2)Notch1免疫组化显示:SB-Adcc(3/6)弥漫中等阳性,c-Adcc(20/20)均阴性;3例恶性腺肌上皮瘤、5例胶原小体病、8例产基质的癌、9例分泌性癌及40例非特殊型三阴型乳腺癌均阴性。(3)FISH检测显示c-Adcc(12/19)MYB基因断裂,NGS检测显示SB-Adcc(3/6)Notch1突变。结论免疫组化MYB、Notch1中等或强的弥漫性表达,可辅助鉴别c-Adcc、SB-Adcc的分型;与恶性腺肌上皮瘤鉴别困难时,可借助分子检测进一步明确。

关键词：乳腺肿瘤 c-Adcc SB-Adcc MYB Notch1

肠源性氧化三甲胺对缺血性心力衰竭大鼠预后的影响

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武警医学 2024年第6期35卷 474-479页

作者：张芯石蕊倪健美刘小伟郭琼王涛王晓静陈少伯武警特色医学中心高原高寒环境及心血管病防治研究所/天津市心血管重塑与靶器官损伤重点实验室天津市中西医结合南开医院

目的评估氧化三甲胺(TMAO)对缺血性心力衰竭大鼠预后的影响。方法 SD大鼠随机分为正常对照组、假手术组、模型(HF)组、干预(HF-DMB)组，其中HF组与HF-DMB组大鼠行结扎法模拟心肌梗死，分别于术后4周(4 W)、8周(8 W)、12周(12 W)评估心... 详细信息

目的评估氧化三甲胺(TMAO)对缺血性心力衰竭大鼠预后的影响。方法 SD大鼠随机分为正常对照组、假手术组、模型(HF)组、干预(HF-DMB)组，其中HF组与HF-DMB组大鼠行结扎法模拟心肌梗死，分别于术后4周(4 W)、8周(8 W)、12周(12 W)评估心脏功能，检测血清TMAO、脑钠肽(BNP)、c反应蛋白(cRP)、内毒素(LPS)、白介素-6(IL-6)等水平，心肌组织进行HE染色及Masson染色，取粪便组织进行16S r DNA高通量测序分析。结果与正常对照组比较，HF组LVEF随时间明显下降，且在8W时下降尤为明显(27.68%±6.41%),HF组各时间点循环TMAO[(9534.02±202.75) nmol/L、(12713.06±243.66) nmol/L、(4970.95±168.15) nmol/L)]、BNP[(37.26±3.41) ng/L、(59.17±3.80) ng/L、(58.83±4.41) ng/L]、cRP[(179.30±13.85)μg/L、(186.40±13.47)μg/L、(187.21±11.35)μg/L]、LPS[(92.45±8.83) ng/L、(90.65±7.5) ng/L、(95.20±4.68) ng/L]、IL-6[(97.57±3.48) pg/ml、(102.60±4.96) pg/ml、(107.70±4.70) pg/ml]等指标浓度较正常对照组明显升高且差异具有统计学意义(P<0.05),HF-DMB组LVEF在各时间点有所回升，且循环中各指标均较HF组有不同程度下降(P<0.05)，且TMAO水平变化与其他指标改变一致;心肌病理染色提示HF-DMB组较HF组心肌细胞存活更多且纤维化程度有所减轻;测序分析中，与HF组比较，HF-DMB组菌落丰度增加，HF-8W组厚壁菌门/拟杆菌门比值下降，而HF-DMB-8W组变化与HF-8W组相反。结论缺血性心力衰竭大鼠循环TMAO变化与传统心力衰竭评价指标变化一致，特异性抑制循环TMAO能够改善心力衰竭多项指标，在心力衰竭的治疗和预后评估中具有一定的临床价值。

关键词：氧化三甲胺心力衰竭 3,3-二甲基-1-丁醇肠道菌群

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胆管癌免疫相关基因的筛选及预后模型构建

现代肿瘤医学 2024年第15期32卷 2788-2795页

作者：李涵琪师煜吴少波陶杰孙昊刘青光孙联康西安交通大学第一附属医院肝胆外科西安交通大学第一附属医院肿瘤内科陕西西安710061 西安交通大学医学部陕西西安710061

目的:基于生物信息学构建胆管癌免疫相关预后模型。方法:首先基于癌症基因组图谱(The cancer Genome Atlas, TcGA)下载胆管癌(cholangiocarcinoma, ccA)的转录组测序(RNA-seq)数据,和从ImmPort网站下载的免疫相关基因(immune-related ge... 详细信息

目的:基于生物信息学构建胆管癌免疫相关预后模型。方法:首先基于癌症基因组图谱(The cancer Genome Atlas, TcGA)下载胆管癌(cholangiocarcinoma, ccA)的转录组测序(RNA-seq)数据,和从ImmPort网站下载的免疫相关基因(immune-related genes, IRGs)取交集。接着通过“DESeq2”R包进行差异分析,进一步用批量单因素cOX、LASSO回归筛选基因,将得到的7个基因和临床信息一起进行多因素cOX分析,构建胆管癌预后模型。使用Kaplan-Meier(K-M)生存曲线、风险因子关联分析、受试者工作特征曲线(receiver operator curve, ROc)进行验证,绘制列线图(Nomogram)。然后,研究模型风险评分和患者临床特征的相关性,并对差异基因进行富集分析。最后,探究高低风险组的免疫特征以及药物敏感性。结果:筛选出3个免疫相关基因(GDNF、FGF11、LcN2)和年龄一起构建预后模型,模型一致性指数(c index value)为0.8。高风险组患者的预后差,免疫细胞浸润程度高,免疫检查点基因表达高,高危免疫亚型占比大,免疫治疗有效率及抗肿瘤药物敏感性低。结论:该研究构建的免疫相关预后模型能够有效预测胆管癌患者的预后以及免疫相关特征和药物治疗有效性。

关键词：免疫相关基因胆管癌预后模型免疫浸润药物敏感性

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小型化频率选择表面研究现状及其应用进展

材料导报 2019年第5期33卷 881-893页

作者：王晖屈绍波空军工程大学研究生院西安710051

频率选择表面是一种在平面内排布的二维周期阵列,由金属贴片单元或孔径单元构成,其对不同频率电磁波呈现不同的滤波响应,被广泛应用于雷达隐身、卫星通讯和电磁兼容等领域。随着应用对技术的要求不断提高,传统频率选择表面难以满足实际... 详细信息

频率选择表面是一种在平面内排布的二维周期阵列,由金属贴片单元或孔径单元构成,其对不同频率电磁波呈现不同的滤波响应,被广泛应用于雷达隐身、卫星通讯和电磁兼容等领域。随着应用对技术的要求不断提高,传统频率选择表面难以满足实际应用。原因有:(1)传统频率选择表面较大的单元尺寸会导致单元谐振时栅瓣过早出现,对传输特性产生不良影响;(2)传统频率选择表面较大的单元尺寸导致其很难在有限空间的区域内以及不规则天线罩上使用,且大尺寸使得现有工艺很难完成贴装布阵。而单元尺寸远小于工作波长的小型化频率选择表面MEFSS的出现为解决上述问题提供了新的思路,其优势日益凸显了自身的价值,逐渐发展成为新的研究方向。最初的小型化频率选择表面通过结构上的交叉、旋绕、连接等模式来达到缩小单元尺寸的目的,最典型的方法就是单元的卷曲、交指设计。为了突破"单元尺寸与谐振波长一致"这一限制,实现小尺寸结构可以控制长波长谐振,通过金属贴片和网格经介质级联,产生了基于容性表面与感性表面耦合技术的小型化频率选择表面。该频率选择表面通过减小单元的有效电尺寸来实现小型化。耦合型双屏小型化频率选择表面主要分为:网栅及其互补结构、网栅和方环贴片阵列结构、方环贴片及其互补结构。这三种典型的耦合双屏结构是研究复杂耦合型小型化频率选择表面结构的基础。由于前两类频率选择表面在实现小型化特性时都无法摆脱周期单元尺寸的制约,若需要更高的小型化需求时则难以实现。而加载无源集总元件(电容器、电感器)的频率选择表面可以增大等效电感L以及等效电容c的值,能够更大程度地缩小单元的有效电尺寸,使得频率选择表面小型化程度更高。为了拓展小型化频率选择表面的功能,在普通小型化频率选择表面单元或介质衬底上加入有源器件(变容二极管和PIN二极管等)或采用特征媒质作为介质衬底等组成有源小型化频率选择表面。该频率选择表面在实现小型化特性的同时,通过对有源器件的调节来控制该频率选择表面的通带开关和变频特性等。本文从单元卷曲和交指设计、基于容性表面与感性表面耦合技术设计、加载无源/有源集总元件技术设计等方面综述了小型化频率选择表面的研究状况。随后,对小型化频率选择表面在实际中的应用进行了介绍并对其未来发展方向进行了展望。

关键词：频率选择表面空间滤波器小型化电磁兼容天线罩

内镜下不同比例聚桂醇泡沫硬化剂注射联合套扎术治疗Ⅱ~Ⅲ度内痔的临床效果

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中国医药科学 2024年第14期14卷 147-150,190页

作者：王家旭覃屹谢榕罗期唐少波张伟伟南宁市第一人民医院消化内科广西南宁535000

目的探讨不同比例的聚桂醇泡沫硬化剂注射联合套扎术治疗Ⅱ~Ⅲ度内痔的有效性及安全性。方法选取2019年12月至2022年12月南宁市第一人民医院消化内科收治的90例内痔患者,按随机数表法将患者分为A、B、c三组。A组为聚桂醇原液组;B组为1∶... 详细信息

目的探讨不同比例的聚桂醇泡沫硬化剂注射联合套扎术治疗Ⅱ~Ⅲ度内痔的有效性及安全性。方法选取2019年12月至2022年12月南宁市第一人民医院消化内科收治的90例内痔患者,按随机数表法将患者分为A、B、c三组。A组为聚桂醇原液组;B组为1∶1聚桂醇泡沫硬化剂组;c组为1∶2聚桂醇泡沫硬化剂组,每组各30例。对各组患者行硬化剂注射和套扎治疗。记录各组手术时间、聚桂醇用量、住院天数、术后疼痛、出血、疗效及异位栓塞、尿潴留、肛门感染等并发症情况。结果三组手术时间、住院天数、并发症发生率比较,差异无统计学意义(P>0.05);三组手术费用、聚桂醇用量、术后疼痛、出血以及疗效比较,差异有统计学意义(Pc组较A组手术费用和聚桂醇用量明显减少,术后疼痛和出血发生更低,疗效明显升高。但B组和c组手术费用、聚桂醇用量、术后疼痛和出血比较,差异无统计学意义(P>0.05)。B组较c组临床疗效明显增高,差异有统计学意义(P<0.05)。结论内镜下1∶1的聚桂醇泡沫硬化注射治疗内痔具有效率高、用量少、费用低、术后出血少、疼痛轻以及并发症发生率低等特点,值得推广。

关键词：内痔聚桂醇泡沫硬化剂消化内镜套扎