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Affinity peptide developed by phage display selection for targeting gastric cancer

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World Journal of Gastroenterology 2012年第17期18卷 2053-2060页

作者： Wen-Jie Zhang Yan-Xia Sui Arun Budha Jian-Bao Zheng Xue-Jun Sun ying-Chun Hou Thomas D Wang shao-ying lu Department of General Surgery The First Affiliated Hospital of Medical School Xian JiaotongUniversity Xi＇an 710061 Shaanxi Province China The Third Hospital of Chengdu Chengdu610031 Sichuan Province China Shaanxi Normal University Xi＇an 710061Shaanxi Province China Department of Medicine Division of Gastroenterology and Hepatology School of Medicine Universityof Michigan Ann Arbor MI 48109 United States

AIM:To develop an affinity peptide that binds to gastric cancer used for the detection of early gastric ***:A peptide screen was performed by biopanning the PhD-12 phage display library,clearing non-specific binders against tumor-adjacent normal appearing gastric mucosa and obtaining selective binding against freshly harvested gastric cancer *** binding of selected peptides was confirmed by bound phage counts,enzyme-linked immunosorbent assay,competitive inhibition,fluorescence microscopy and semi-quantitative analysis on immunohistochemistry using different types of cancer ***:Approximately 92.8% of the non-specific phage clones were subtracted from the original phage library after two rounds of biopanning against normal-appearing gastric *** the third round of positive screening,the peptide sequence AADNAKTKSFPV(AAD) appeared in 25%(12/48) of the analyzed *** the control peptide,these values were 6.8 ± 2.3,5.1 ± 1.7,3.5 ± 2.1,4.6 ± 1.9 and 1.1 ± 0.5,*** values for AAD peptide were statistically signif icant(P < 0.01) for gastric cancer as compared with other histological classif ications and control ***:A novel peptide is discovered to have a specific binding activity to gastric cancer,and can be used to distinguish neoplastic from normal gastric mucosa,demonstrating the potential for early cancer detection on endoscopy.

关键词： Gastric cancer Peptide Phage library Molecular imaging Early detection Immunohistochemistry Enzyme-linked immunosorbent assay

Percutaneous suture technique with ProGlide to manage vascular access pseudoaneurysm after percutaneous coronary intervention procedure: A case report

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Chinese Journal of Traumatology 2020年第1期23卷 34-37页

作者： Wei Liu Chao Liu shao-ying lu Department of Vascular Surgery The First Affiliated Hospital of Medical SchoolXi'an Jiaotong UniversityXi'an 710061China

Iatrogenic femoral artery pseudoaneurysm is a common complication of the endovascular *** compression and thrombin injection are the conventional techniques to occlude the ***,there are still some failed cases that applied these treatment *** aim of the study is to seek a potential and alternative method with ProGlide system to close the *** April 2018 to February 2019,2 patients with iatrogenic pseudoaneurysm of the superficial femoral were treated with the suture-base closure device–*** punctured the pseudoaneurysm and placed a 6-F sheath,the guide wire was placed in the right femoral artery via the access of the pseudoaneurysm *** the pseudoaneurysm neck was sutured by ProGlide to occlude the blood supply to the *** 2 patients were cured with no complications and complaints,which revealed that percutaneous suture technique with ProGlide at the neck level of pseudoaneurysm provides a novel method for the management of vascular access pseudoaneurysm,especially in those with a wide and short neck.

关键词： Pseudoaneurysm Percutaneous suture ProGlide

Design, Synthesis, and Activities of Novel Derivativesof Isophthalamide and Benzene-1,3-disulfonamide

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Chemical Research in Chinese Universities 2006年第3期22卷 356-359页

作者： LIU Xiu-jie WANG Song-qing ZHANG jing ZHANG Feng-xia LI Gui-zhu WANG Bao-jie shao ying-lu ZHANG Li-guang FANG Lin CHENG Mao-sheng The College of Pharmaceutical Engineering Shenyang Pharmaceutical University Shenyang 110016 P.R. China The College of Pharmaceutical and Biotechnology Tianjin University Tianjin 300072 P. R. China The School of Biology and Chemistry Tianjin University of Technology Tianjin 300191 P. R. China

Based on the antiplatelet aggregation mechanism and the bioisosterism principle of the reference drug picotamide, thirteen novel derivatives of arylamide and arylsulfonamide were designed and prepared. The biological activities of these derivatives were investigated. The chemical structures of the target compounds were confirmed by ^1H NMR and IR. The in vitro activities of antiplatelet aggregation of the thirteen target compounds were assessed by Bore＇s method. Compounds 2b and 8h have significant antiplatelet aggregation activities, which are superior to the corresponding activity of Picotamide.

关键词： Thromboxane Antiplatelet Isophthalamide Disulfonamide Synthesis

Iatrogenic femoral artery pseudoaneurysm surgically repaired with combined bovine pericardial roll and autologous great saphenous vein grafts

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Chinese Journal of Traumatology 2021年第2期24卷 75-78页

作者： Xun Huang Hui Cai shao-ying lu Department of Vascular Surgery The First Affiliated Hospital of Medical SchoolXi’an Jiaotong UniversityXi’an710061China

Iatrogenic femoral artery pseudoaneurysm caused by invasive procedures is one of the common complications for endovascular *** present a case of a young male with a complex iatrogenic femoral artery pseudoaneurysm as a result of iatrogenic femoral artery *** defective femoral artery was repaired with combined bovine pericardial tube and autologous great saphenous vein *** tomography angiography showed the grafts were still patent one year after the surgery.

关键词： Femoral artery Pseudoaneurysm Restoration

Overcoming chemoresistance in non-angiogenic colorectal cancer by metformin via inhibiting endothelial apoptosis and vascular immaturity

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Journal of Pharmaceutical Analysis 2023年第3期13卷 262-275页

作者： Guang-Yue Li Shu-Jing Zhang Dong Xue Yue-Qi Feng Yan Li Xun Huang Qiang Cui Bo Wang Jun Feng Tao Bao Pei-Jun Liu shao-ying lu Ji-Chang Wang Department of Science and Technology The First Affiliated Hospital of Xi'an Jiaotong UniversityXi'an710061China Department of Vascular Surgery The First Affiliated Hospital of Xi'an Jiaotong UniversityXi'an710061China Department of General Surgery The First Affiliated Hospital of Xi'an Jiaotong UniversityXi'an710061China Center for Translational Medicine The First Affiliated Hospital of Xi'an Jiaotong UniversityXi'an710061China Department of Pharmacy Xi'an Jiaotong University Health Science CenterXi'an710061China

The development of chemoresistance which results in a poor prognosis often renders current treatments for colorectal cancer(CRC).In this study,we identified reduced microvessel density(MVD)and vascular immaturity resulting from endothelial apoptosis as therapeutic targets for overcoming *** focused on the effect of metformin on MVD,vascular maturity,and endothelial apoptosis of CRCs with a non-angiogenic phenotype,and further investigated its effect in overcoming *** situ transplanted cancer models were established to compare MVD,endothelial apoptosis and vascular maturity,and function in tumors from metformin-and vehicle-treated *** in vitro co-culture system was used to observe the effects of metformin on tumor cell-induced endothelial *** sequencing was performed for genetic ***-angiogenic CRC developed independently of angiogenesis and was characterized by vascular leakage,immaturity,reduced MVD,and *** phenomenon had also been observed in human ***,non-angiogenic CRCs showed a worse response to chemotherapeutic drugs in vivo than in *** suppressing endothelial apoptosis,metformin sensitized non-angiogenic CRCs to chemo-drugs via elevation of MVD and improvement of vascular *** results showed that endothelial apoptosis was induced by tumor cells via activation of caspase signaling,which was abrogated by metformin *** findings provide pre-clinical evidence for the involvement of endothelial apoptosis and subsequent vascular immaturity in the chemoresistance of non-angiogenic *** suppressing endothelial apoptosis,metformin restores vascular maturity and function and sensitizes CRC to chemotherapeutic drugs via a vascular mechanism.

关键词： Metformin Colorectal cancer Non-angiogenic Endothelial apoptosis Vascular immaturity

Storage time affects the level and diagnostic efficacy of plasma biomarkers for neurodegenerative diseases

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Neural Regeneration Research 2025年第8期20卷 2373-2381页

作者： Lifang Zhao Mingkai Zhang Qimeng Li Xuemin Wang Jie lu ying Han Yanning Cai Department of Clinical Biobank Xuanwu HospitalCapital Medical UniversityBeijingChina Beijing Geriatric Medical Research Center BeijingChina Department of Neurology Xuanwu HospitalCapital Medical UniversityBeijingChina Department of Radiology and Nuclear Medicine Xuanwu HospitalCapital Medical UniversityBeijingChina Beijing Key Laboratory of Magnetic Resonance Imaging and Brain Informatics BeijingChina Key Laboratory of Neurodegenerative diseases Ministry of EducationBeijingChina School of Biomedical Engineering Hainan UniversityHaikouHainan ProvinceChina Center of Alzheimer’s Disease Beijing Institute for Brain DisordersBeijingChina National Clinical Research Center for Geriatric Diseases BeijingChina Institute of Biomedical Engineering Shenzhen Bay LaboratoryGaoke Innovation CenterShenzhenGuangdong ProvinceChina Department of Neurobiology Xuanwu HospitalCapital Medical UniversityBeijingChina

Several promising plasma biomarker proteins,such as amyloid-β(Aβ),tau,neurofilament light chain,and glial fibrillary acidic protein,are widely used for the diagnosis of neurodegenerative ***,little is known about the long-term stability of these biomarker proteins in plasma samples stored at-80°*** aimed to explore how storage time would affect the diagnostic accuracy of these biomarkers using a large *** samples from 229 cognitively unimpaired individuals,encompassing healthy controls and those experiencing subjective cognitive decline,as well as 99 patients with cognitive impairment,comprising those with mild cognitive impairment and dementia,were acquired from the Sino Longitudinal Study on Cognitive Decline *** samples were stored at-80°C for up to 6 years before being used in this *** results showed that plasma levels of Aβ42,Aβ40,neurofilament light chain,and glial fibrillary acidic protein were not significantly correlated with sample storage ***,the level of total tau showed a negative correlation with sample storage ***,in individuals without cognitive impairment,plasma levels of total protein and tau phosphorylated protein threonine 181(p-tau181)also showed a negative correlation with sample storage *** was not observed in individuals with cognitive ***,we speculate that the diagnostic accuracy of plasma p-tau181 and the p-tau181 to total tau ratio may be influenced by sample storage ***,caution is advised when using these plasma biomarkers for the identification of neurodegenerative diseases,such as Alzheimer's ***,in cohort studies,it is important to consider the impact of storage time on the overall results.

关键词： Alzheimer’s disease amyloid-β diagnostic ability glial fibrillary acidic protein neurodegeneration neurofilament light chain plasma biomarkers single molecule array storage time tau

Catalyst–Support Interaction in Polyaniline‑Supported Ni_(3)Fe Oxide to Boost Oxygen Evolution Activities for Rechargeable Zn‑Air Batteries

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Nano-Micro Letters 2025年第1期17卷 176-190页

作者： Xiaohong Zou Qian lu Mingcong Tang Jie Wu Kouer Zhang Wenzhi Li Yunxia Hu Xiaomin Xu Xiao Zhang Zongping shao Liang An Department of Mechanical Engineering The Hong Kong Polytechnic UniversityHung HomKowloonHong Kong SARPeople’s Republic of China Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control Jiangsu Collaborative Innovation Center of Atmospheric Environment and Equipment TechnologySchool of Environmental Science and TechnologyNanjing University of Information Science and TechnologyNanjing 210044People’s Republic of China Department of Chemistry The Chinese University of Hong KongMa Lin BuildingShatinHong Kong SAR 999077People’s Republic of China WA School of Mines:Minerals Energy and Chemical Engineering(WASM‑MECE)Curtin UniversityPerthWA 6102Australia Research Institute for Advanced Manufacturing The Hong Kong Polytechnic UniversityHung HomKowloonHong Kong SARPeople’s Republic of China Research Institute for Smart Energy The Hong Kong Polytechnic UniversityHung HomKowloonHong Kong SARPeople’s Republic of China

Catalyst–support interaction plays a crucial role in improving the catalytic activity of oxygen evolution reaction(OER).Here we modulate the catalyst–support interaction in polyaniline-supported Ni_(3)Fe oxide(Ni_(3)Fe oxide/PANI)with a robust hetero-interface,which significantly improves oxygen evolution activities with an overpotential of 270 mV at 10 mA cm^(-2)and specific activity of 2.08 mA cm_(ECSA)^(-2)at overpotential of 300 mV,3.84-fold that of Ni_(3)Fe *** is revealed that the catalyst–support interaction between Ni_(3)Fe oxide and PANI support enhances the Ni–O covalency via the interfacial Ni–N bond,thus promoting the charge and mass transfer on Ni_(3)Fe *** the excellent activity and stability,rechargeable Zn-air batteries with optimum Ni_(3)Fe oxide/PANI are assembled,delivering a low charge voltage of 1.95 V to cycle for 400 h at 10 mA cm^(-2).The regulation of the effect of catalyst–support interaction on catalytic activity provides new possibilities for the future design of highly efficient OER catalysts.

关键词： Catalyst-support interaction Supported catalysts Heterointerface Oxygen evolution reaction Zn-air batteries

Inhibitory gamma-aminobutyric acidergic neurons in the anterior cingulate cortex participate in the comorbidity of pain and emotion

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Neural Regeneration Research 2025年第10期20卷 2838-2854页

作者： lu Guan Mengting Qiu Na Li Zhengxiang Zhou Ru Ye Liyan Zhong Yashuang Xu Junhui Ren Yi Liang Xiaomei shao Jianqiao Fang Junfan Fang Junying Du Department of Neurobiology and Acupuncture Research Third School of Clinical MedicineZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Key Laboratory for Research of Acupuncture Treatment and Transformation of Emotional Diseases Third School of Clinical MedicineZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Fuchun Community Health Service Center of Fuyang District HangzhouZhejiang ProvinceChina

Pain is often comorbid with emotional disorders such as anxiety and *** of the anterior cingulate cortex has been implicated in pain and pain-related negative emotions that arise from impairments in inhibitory gamma-aminobutyric acid *** review primarily aims to outline the main circuitry(including the input and output connectivity)of the anterior cingulate cortex and classification and functions of different gamma-aminobutyric acidergic neurons;it also describes the neurotransmitters/neuromodulators affecting these neurons,their intercommunication with other neurons,and their importance in mental comorbidities associated with chronic pain *** understanding on their role in pain-related mental comorbidities may facilitate the development of more effective treatments for these ***,the mechanisms that regulate gamma-aminobutyric acidergic systems remain *** is also unclear as to whether the mechanisms are presynaptic or *** exploration of the complexities of this system may reveal new pathways for research and drug development.

关键词： anterior cingulate cortex anxiety chronic pain circuit communication comorbidity depression gamma-aminobutyric acidergic neurons parvalbumin neurons synaptic transmission

基于地黄转录组数据的SNP标记开发与地黄指纹图谱构建

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生物技术通报 2019年第11期35卷 224-230页

作者：郭萌萌周延清段红英杨珂邵露营河南师范大学生命科学学院新乡453007

地黄(Rehmannia glutinosa)是一种具有较高药用价值和经济价值的植物。准确的品种鉴定对地黄种质管理和育种至关重要,使用SNP分子标记来鉴定地黄种质和构建指纹图谱对地黄分子标记育种提供了新方法。利用地黄转录组数据和SRA数据库中的... 详细信息

地黄(Rehmannia glutinosa)是一种具有较高药用价值和经济价值的植物。准确的品种鉴定对地黄种质管理和育种至关重要,使用SNP分子标记来鉴定地黄种质和构建指纹图谱对地黄分子标记育种提供了新方法。利用地黄转录组数据和SRA数据库中的3个地黄转录组数据比对,寻找候选SNP,用PCR技术扩增和序列分析研究28个地黄品种候选SNP变异。从地黄转录组数据中获得了102075条Unigenes,其中共有SNP位点35339个,发生频率为0.51/kb;从中随机选取40个候选SNP位点,设计引物39对,用PCR和序列分析从中筛选出7对好的SNP引物,包含8个多态性好的SNP位点;利用最终筛选出的8个SNP位点构建地黄指纹图谱,可以将17个不同地黄种质区分开,可用于地黄种间和种内品种的鉴定。

关键词：地黄转录组 SNP标记开发指纹图谱品种鉴定