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Identification of Dysregulated microRNAs in Glioma Using RNA sequencing

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Current Medical Science 2021年第2期41卷 356-367页

作者： Chang LIU Ying-ying GE xiao-xun XIE Bin LUO Ning SHEN Xing-sheng LIAO Shui-qing BI Tao XU shao-wen xiao Qing-mei ZHANG Department of Neurosurgery the First Affiliated HospitalGuangxi Medical UniversityNanning 530021China School of Pre-clinical Medicine Guangxi Medical UniversiryNanning 530021China Department of Stomatology the People's Hospital of Guangxi Zhuang Atutonomous RegionNanning 530021China

Glioma is the most common malignant brain tumor in central nervous *** advances in the treatment of glioma such as surgery and chemoradiotherapy,most patients are easy to relapse,resulting in adverse clinical ***,effective molecular=targeting treatment may be one of attractive strategies for glioma *** dysregulated microRNAs(miRNAs),one of the candidates of therapeutic targets,are believed to play an important role in the progression of *** this study,we aimed to examine the expression profile of miRNAs in glioma and provide a reference for glioma ***,expression profile of miRNAs in 5 normal brain tissues,5 low-grade glioma(LGG)tissues and 5 glioblastoma(GBM)tissues was detected by RNA sequencing(RNA-seq).Next,the target genes of differentially expressed miRNAs(DEmiRNAs)were predicted and then GO enrichment and KEGG pathway analysis performed by ***,10 miRNAs which were significantly up-or down-regulated both in GBM and LGG were validated by real-time quantitative PCR(qRT-PCR).RNA-seq results indicated a number of DEmiRNAs in *** were 64 up-regulated miRNAs and 17 down-regulated miRNAs n LGG,and 181 up-regulated miRNAs and 124 down-regulated miRNAs in GBM,*** analysis showed that the target genes of these DEmiRNAs were enriched in various biological processes and signaling pathways such as cell metabolic and developmental *** DEmiRNAs were further confirmed by ***-10b-5p,miRNA-92b-3p and miRNA-455-5p were significantly up-regulated in both GBM and LGG;while miRNA-542-3p was significantly up-regulated in LGG;miRNA-184 and miRNA-206 were significantly down-regulated in both GBM and LGG;miRNA-766-5p and miRNA-1-3p were significantly down-regulated in *** subject of our study demonstrated several dysregulated miRNAs may serve as a potential therapeutic target for gl ioma.

关键词： glioma microRNA RNA-sequencing

High Expression of Fibronectin 1 Predicts a Poor Prognosis in Glioblastoma

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Current Medical Science 2022年第5期42卷 1055-1065页

作者： Song WU Chang LIU Xing WEI Wei-xia NONG Li-na LIN Feng LI xiao-xun XIE Xing-sheng LIAO Bin LUO Qing-mei ZHANG shao-wen xiao Department of Neurosurgery the First Affiliated HospitalGuangxi Medical UniversityNanning530021China Department of Histology and Embryology School of Pre-clinical MedicineGuangxi Medical UniversityNanning530021China Central Laboratory of Pre-Clinical Medicine(Key Laboratory of Guangxi Colleges and Universities) Guangxi Medical UniversityNanning530021China Key Laboratory of Early Prevention and Treatment of Regional High Frequency Tumor(Guangxi Medical University) Ministry of EducationNanning530021China Department of Neurology the First Affiliated HospitalGuangxi Medical UniversityNanning530021China

Objective Glioblastoma multiforme(GBM),the most malignant intracranial neoplasm,is associated with a high mortality and recurrence rate due to the aggressive nature and heterogeneity of the *** of the molecular markers involved in the tumorigenesis of GBM are essential in prognosis,diagnosis,and *** to the limitations of therapeutic effects,this study aims to explore novel biomarkers with prognostic value and to provide new insights into therapeutic *** The expression profile of mRNAs in GBM was detected by RNA-sequencing,and differentially expressed genes were identified by integrating the data from RNA-seq results and the GEPIA2 *** the total 40 hub genes,FN1,P4HB,and PPIB showed prognostic significance based on both GEPIA2 and CGGA *** validation of FN1,P4HB,and PPIB expression by qPCR and correlation analysis with clinicopathological features were performed in 41 GBM tissues from our *** Kaplan-Meier analysis revealed that FN1 and P4HB expressions levels were related to the overall survival(OS)of GBM patients(P<0.05).Multivariate analysis showed that FN1 overexpression(HR=9.199,P=0.002)was an independent and unfavorable prognostic factor for GBM *** median survival time was 8.5 months and 21 months for high and low expressions of FN1,*** It was suggested that FN1 could be an ideal target for prognosis and a potential therapeutic target in GBM.

关键词： glioblastoma FN1 overall survival prognosis

FMR1NB Involved in Glioma Tumorigenesis Is a Promising Target for Prognosis and Therapy

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Current Medical Science 2022年第4期42卷 803-816页

作者： Shui-qing BI Ya PENG Zong-dang WEI Sheng-zhong YAO Bin LUO Ying-ying GE xiao-xun XIE Wei-xia NONG Chang LIU shao-wen xiao Qing-mei ZHANG Department of Neurosurgery the First Affiliated Hospital of Guangxi Medical UniversityNanning 530021China Department of Histology and Embryology School of Pre-Clinical MedicineGuangxi Medical UniversityNanning 530021China Central Laboratory School of Pre-Clinical MedicineGuangxi Medical UniversityNanning 530021China

Objective:Cancer/testis antigen FMR1NB is aberrantly expressed in various types of cancer,but not in normal tissues except for *** study aimed to investigate the expression and functional role of FMR1NB in ***:The expression of FMR1NB mRNA and protein was determined using RT-PCR and immunohistochemistry,respectively,in glioma specimens from 83 patients at *** effects of siRNA-mediated FMR1NB silencing on malignant biological behaviors were evaluated in glioma cell lines Al 72 and ***:FMR1NB mRNA and protein expression was detected in 58.8%(77/131)and 46.34%(57/123)of glioma tissues,***1NB protein was positively correlated with World Health Organization grade and found to be an independent prognostic marker for poor *** of FMR1NB induced apoptosis and suppressed proliferation,adhesion,migration,and invasion by modulating the expression of cyclin A,CDK2,caspase-3,E-cadherin,and N-cadherin in A172 and U251 ***:Our findings suggest that FMR1NB contributes to the tumorigenesis of glioma cells and may represent a potential prognostic biomarker and an attractive therapeutic target in glioma.

关键词： cancer/testis antigen FMR1NB glioma

Involvement of X-chromosome Reactivation in Augmenting Cancer Testis Antigens Expression:A Hypothesis

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2018年第1期38卷 19-25页

作者： Chang LIU Bin LUO xiao-xun XIE Xing-sheng LIAO Jun FU Ying-ying GE Xi-sheng LI Gao-shui GUO Ning SHEN shao-wen xiao Qing-mei ZHANG Department of Neurosurgery the First Affiliated Hospital of Guangxi Medical UniversityNanning 530021China Department of Histology and Embryology Guangxi Medical UniversityNanning 530021China Central Laboratory School of Pre-Clinical MedicineGuangxi Medical UniversityNanning 530021China Department of Oral and Maxillofacial Surgery The People's Hospital of Guangxi Zhuang Autonomous RegionNanning 530021China

Cancer testis antigens(CTAs)are attractive targets for tumor immunotherapy because of their tumor-specific *** more than half of confirmed CTAs are located on the X-chromosome,we asked whether there is a link between CTA expression and *** reports have shown that reactivation of the inactive X-chromosome,known as X-chromosome reactivation(XCR),a unique phenomenon that exists in many high-risk tumors in women,can transform the expression of many X-linked genes from monoallelic to *** this review,we discuss the link between CTA and XCR with the hopes of providing some novel insights into tumor biology.

关键词： cancer testis antigen X-chromosome reactivation tumor immunotherapy

Intracranial foreign body through the sagittal sinus: case report and review of literature

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Chinese Neurosurgical Journal 2016年第3期2卷 -页

作者： Roodrajeetsing Gopaul wen shao xiao Jun Yan Dang Zong Wei Department of Neurosurgery Guangxi Medical University First Affiliated Hospital 6 Shuang Yong Road 530021 Nanning Guangxi Zhuang Autonomous People's Republic of China Department of Neurosurgery

Objective:To investigate therapeutic effect of surgery that removes an intracranial foreign body through the intracranial sagittal sinus with help of intraoperative navigation and intraoperative fluorescein ***:We performed operation on a patient with intracranial foreign *** guidance of intraoperative navigation,combined with intraoperative fluorescein angiography to avoid sagittal sinus damage,we opened the dura mater and removed the foreign body with minimally invasive ***:Surgical positioning was very accurate with neuronavigation,intraoperative fluorescein angiography displayed the venous drainage along the sagittal sinus in order to prevent its rupture;the intracranial foreign body was removed successfully without any post-operative ***:Intraoperative navigation and intraoperative fluorescein angiography technologies to guide the removal of the foreign metal body inserted through the sagittal sinus have the following advantages:Accurate positioning,minimally invasive,reliable curative effect,quick postoperative recovery and overall fewer complications.

关键词： Intracranial foreign body The sagittal sinus Surgical technologies

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Intraoperative rupture of blood blister-like aneurysm: a case report and review of literature

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Chinese Neurosurgical Journal 2016年第1期2卷 -页

作者： Roodrajeetsing Gopaul wen shao xiao Jun Yan Dang Zong Wei Department of Neurosurgery First Affiliated Hospital Guangxi Medical University 6 Shuang Yong Road 530021 Nanning Guangxi Zhuang Autonomous People's Republic of China

Blood blister-like aneurysms (BBLAs) are aneurysms from the non-branching sites of the internal carotid artery (ICA).Though rare lesions,they pose a high risk of intraoperative aneurysmal *** treatment of these types of aneurysms has been debatable,but surgical approach is the ultimate rescue *** of the intraoperative ruptured BBLA has been reported scarcely in literature,but no review of these cases has ever been *** here present our experience of a case of BBLA intraoperative rupture requiring microsuture of the ICA and conduct meticulous review of all similar cases.

关键词： Blood blister-like aneurysm (BBLA) Angiogram-negative subarachnoid hemorrhage (SAH) Intraoperative rupture

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融合属性特征的行人重识别方法

自动化学报 2022年第2期48卷 564-571页

作者：邵晓雯帅惠刘青山南京信息工程大学自动化学院江苏省大数据分析技术重点实验室南京210044

行人重识别旨在跨监控设备下检索出特定的行人目标.由于不同的行人可能具有相似的外观,因此要求行人重识别模型能够捕捉到充足的细粒度特征.本文提出一种融合属性特征的行人重识别的深度网络方法,将行人重识别和属性识别集成在分类网络... 详细信息

行人重识别旨在跨监控设备下检索出特定的行人目标.由于不同的行人可能具有相似的外观,因此要求行人重识别模型能够捕捉到充足的细粒度特征.本文提出一种融合属性特征的行人重识别的深度网络方法,将行人重识别和属性识别集成在分类网络中,进行端到端的多任务学习.此外,对于每张输入图片,网络自适应地生成对应于每个属性的权重,并将所有属性的特征以加权求和的方式结合起来,与全局特征一起用于行人重识别任务.全局特征关注行人的整体外观,而属性特征关注细节区域,两者相互补充可以对行人进行更全面的描述.在行人重识别的主流数据集DukeMTMC-reID和Market-1501上的实验结果表明了本文方法的有效性,平均精度均值(Mean average precision,mAP)分别达到了74.2%和83.5%,Rank-1值分别达到了87.1%和93.6%.此外,在这两个数据集上的属性识别也得到了比较好的结果.

关键词：行人重识别属性识别深度学习自适应权重

OY-TES-1在肝细胞癌中的表达及抗体血清学分析

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世界华人消化杂志 2009年第32期17卷 3307-3312页

作者：范蓉黄巍肖绍文罗彬何少健罗国容谢小薰广西中医学院组织学与胚胎学教研室广西壮族自治区南宁市530001 广西医科大学组织学与胚胎学教研室广西壮族自治区南宁市530021

目的:了解OY-TES-1在肝细胞癌(HCC)中的表达及其抗体出现情况,探讨将OY-TES-1用于肿瘤免疫诊疗的可能性.方法:利用实时定量PCR和免疫组织化学技术,从 mRNA和蛋白质两个水平研究OY-TES-1在HCC中的表达特点;采用酶联免疫吸附实验检测HCC... 详细信息

目的:了解OY-TES-1在肝细胞癌(HCC)中的表达及其抗体出现情况,探讨将OY-TES-1用于肿瘤免疫诊疗的可能性.方法:利用实时定量PCR和免疫组织化学技术,从 mRNA和蛋白质两个水平研究OY-TES-1在HCC中的表达特点;采用酶联免疫吸附实验检测HCC患者及正常人血清中相应的抗体,并对其临床意义做初步的分析.结果:经实时定量PCR检测,HCC中OY-TES-1 mRNA阳性率为73.21%(41/56),癌旁阳性率为64.86%(24/37),有17对HCC及配对癌旁组织均表达阳性,OY-TES-1 mRNA在HCC及癌旁组织两者的表达阳性率无差异(P>0.05);在37对HCC及配对癌旁组织中,HCC中OY-TES-1 mRNA水平明显高于对应的癌旁组织,其差异与病理分级有关(P<0.05).HCC中OY-TES-1蛋白阳性率为40%(4/10).血清学检测未发现76例正常人和17例肝硬化患者血清中有相应抗体,HCC患者血清抗体阳性率为20%(12/58),OY-TES-1血清抗体的出现与HCC患者的年龄、性别等临床资料无相关.结论:OY-TES-1在HCC中有较高的表达频率及表达水平,该蛋白具有较强的免疫原性,他有望作为用于HCC辅助诊断及免疫治疗的肿瘤抗原.

关键词：癌-睾丸抗原 OY—TES—1基因基因表达血清

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超像素特征的运动目标检测算法

控制理论与应用 2017年第12期34卷 1568-1574页

作者：胡昭华张维新邵晓雯南京信息工程大学电子与信息工程学院江苏南京210044 南京信息工程大学江苏省大气环境与装备技术协同创新中心江苏南京210044

在像素级的背景建模方法中,由于其反映的只是时间上的连续性,没有考虑到空间上的相关性,所以会导致检测目标不完整,或检测目标呈碎片化的结果,不利于后续的识别或跟踪.为此,本文首先针对ViBe算法对于动态背景不鲁棒的问题进行了改进,利... 详细信息

在像素级的背景建模方法中,由于其反映的只是时间上的连续性,没有考虑到空间上的相关性,所以会导致检测目标不完整,或检测目标呈碎片化的结果,不利于后续的识别或跟踪.为此,本文首先针对ViBe算法对于动态背景不鲁棒的问题进行了改进,利用样本集的标准差作为动态背景度量值,实时更新距离阈值和背景模型更新率,达到对动态背景的鲁棒性;同时引入了超像素特征,提出了基于超像素特征的运动目标检测算法.由于超像素分割具有较好的边缘信息同时超像素数目可控,所以根据SLIC0超像素分割算法提取超像素特征,将超像素块中的像素均值作为超像素特征值,并引入到改进的ViBe算法框架中;由于超像素分割的数目并不是固定不变的,所以本文使用初始种子点位置的超像素特征构建背景模型并进行运动目标的检测.实验表明,该方法检测结果具有良好的目标边缘信息并可以有效抑制动态背景的干扰.

关键词： ViBe算法超像素运动目标检测 SLIC0算法动态背景