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Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第2期17卷 110-126页

作者： Hong-cui liU Bing-qiang YUAN shao-nan li Institute of Pesticide and Environmental Toxicology Zhejiang University Institute of Technology Innovation Zhejiang University

To yield cholinesterase（ChE） from prokaryotic expression, the ChE gene that belongs to Daphnia magna was amplified by reverse transcription-polymerase chain reaction（RT-PCR） using forward primer 5＇-CCCYGGNGCSAT GATGTG-3＇ and reverse primer 5＇-GYAAGTTRGCCCAATATCT-3＇. To express the gene, one sequence of the amplified DNA, which was able to encode a putative protein containing two conserved carboxylesterase domains, was connected to the prokaryotic expression vector PET-29a（＋）. The recombinant vector was transformed into Escherichia coil BL21（DE3）. Protein expression was induced by isopropy-D-thiogalactoside. The expressed ChE was used as an immunogen to immunize BALB/c mice. The obtained antibodies were tested for their specificity towards crude enzymes from species such as Alona milleri, Macrobrachium nipponense, Bombyx mori, Chironomus kiiensis, Apis mellifera, Eisenia foetida, Brachydanio rerio, and Xenopus laevis. Results indicated that the antibodies had specificity suitable for detecting ChE in Daphnia magna. A type of indirect and non-competitive enzyme-linked immunosorbent assay（IN-EliSA） was used to test the immunoreactive content of ChE（ChE-IR） in Daphina magna. The detection limit of the IN-EliSA was found to be 14.5 ng/ml at an antiserum dilution of 1：22 000. Results from tests on Daphnia magna exposed to sublethal concentrations of triazophos indicated a maximal induction of 57.2% in terms of ChE-IR on the second day after the animals were exposed to a concentration of 2.10 μg/L triazophos. Testing on animals acclimatized to a temperature of 16 °C indicated that ChE-IR was induced by 16.9% compared with the ChE-IR content detected at 21 °C, and the rate of induction was 25.6% at 10 °C. The IN-EliSA was also used to test the stability of ChE-IR in collected samples. Repeated freezing and thawing had no influence on the outcome of the test. All these results suggest that the polyclonal antibodies developed against the recombinant ChE are as efficient as th

关键词： Daphnia magna Cholinesterase(ChE) Polymerase chain reaction(PCR) Recombinant protein ChE Enzyme-linked immunosorbent assay(EliSA) Triazophos

Effects of White-Rot Fungi Composting on Wood Powder-Chicken Manure Mixture

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Animal Husbandry and Feed Science 2009年第6期1卷 42-46页

作者： HU Chang-qing HE Xiao-yi shao Wei shao Sai-nan ZHANG li-lin Ningbo Municipal Key Lab of Microorganism and Environmental Engineering Ningbo 315100 China

[ Objective] To explore the possibility of treating livestock manure with white-rot fungi. [Method] The wood powder-chicken manure mixture was respectively composted by three kinds of white-rot fungi, the weight of lignin, cellulose, protein, fat, ash and crude polysaccharide as well as the mycelial growth was determined during this process. [ Result] The mixture was better composted with white-rot fungi than the control. The best effect was achieved in P. ostreatus composting group. In this group, the rate of weight reduction, lignin degradation and cellulose degradation were respectively 15.68% （6.79 times as great as that of the control group）, 39.92% （6.54 times as great as that of the control group） and 32.26% （2.77 times as great as that of the control group）. The weight of protein and fat were increased by 31.68% and 146.58%, respectively. The content of crude polysaccharide was 2.43%. No crude polysaccharide was detected, and the weight of protein and fat decreased by 21.96% and 70.99%, respectively. [ Conclusion] It is feasible to compost livestock and poultry manure with white-rot fungi.

关键词： White-rot fungi Wood powder-chicken manure mixture lignin Cellulose Crude polysaccharide

Constraints on holographic QCD phase transitions from PTA observations

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 71-78页

作者： Song He li li Sai Wang shao-Jiang Wang Center for Theoretical Physics and College of Physics Jilin University School of Physical Science and Technology Ningbo University Max Planck Institute for Gravitational Physics (Albert Einstein Institute) CAS Key Laboratory of Theoretical Physics Institute of Theoretical Physics Chinese Academy of Sciences School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences (UCAS) School of Physical Sciences University of Chinese Academy of Sciences (UCAS) Theoretical Physics Division Institute of High Energy Physics Chinese Academy of Sciences

The underlying physics of QCD phase transition in the early Universe remains largely unknown due to its strong-coupling nature during the quark-gluon plasma/hadron gas transition, yet a holographic model has been proposed to quantitatively fit the lattice QCD data while with its duration of the first-order phase transition(FoPT) left undetermined. At specific baryon chemical potential, the first-order QCD phase transition agrees with the observational constraint of baryon asymmetry. It, therefore, provides a scenario for phase transition gravitational waves(GWs) within the standard model of particle physics. If these background GWs could contribute dominantly to the recently claimed common-spectrum red noise from pulsar timing array(PTA) observations, the duration of this FoPT can be well constrained, and the associated primordial black holes are still allowed by current observations.

关键词： pulsar timing array QCD phase transition gravitational waves primordial black holes

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模拟退火神经网络模型在地下水质评价中应用

辽宁工程技术大学学报（自然科学版） 2005年第Z1期24卷 244-246页

作者：邵立南刘志斌辽宁工程技术大学资源与环境工程学院辽宁阜新123000

采用模拟退火算法与神经网络相结合的方法建立了地下水水质评价的SA-BP神经网络模型,并对阜新新邱露天煤矿地下水水质进行了评价。结果表明,模拟退火算法具有快速学习网络权重和全局搜索的超强能力,有效地解决了BP算法的局部收敛的问题... 详细信息

采用模拟退火算法与神经网络相结合的方法建立了地下水水质评价的SA-BP神经网络模型,并对阜新新邱露天煤矿地下水水质进行了评价。结果表明,模拟退火算法具有快速学习网络权重和全局搜索的超强能力,有效地解决了BP算法的局部收敛的问题。应用此方法评价地下水水质简便可靠,预测精度高,具有通用性和客观性等优点。

关键词： SA-BP神经网络模型模拟退火算法 BP算法

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高浊高铁锰矿井水回用处理实验研究

中国矿业大学学报 2008年第1期37卷 125-128页

作者：李丹何绪文王春荣李焱邵立南中国矿业大学化学与环境工程学院北京100083

针对高浊高铁锰矿井水的水质特性，分别采用普通石英砂、普通锰砂以及经过KMnO4溶液改性后的石英砂和锰砂等滤料进行除浊、除铁和除锰的过滤实验研究．结果表明，以上几种滤料具有良好的除浊效果，去除率达99％以上；且均能有效除铁，... 详细信息

针对高浊高铁锰矿井水的水质特性，分别采用普通石英砂、普通锰砂以及经过KMnO4溶液改性后的石英砂和锰砂等滤料进行除浊、除铁和除锰的过滤实验研究．结果表明，以上几种滤料具有良好的除浊效果，去除率达99％以上；且均能有效除铁，对铁的去除率在93％以上．经5％KMnO4改性后的锰砂滤料（滤速为6～8m／h）具有较好的去除铁和锰的效果，出水铁和锰浓度都在0．1mg／L以下，能够满足回用水的水质要求，且滤池启动快、除锰效果持续时间长．

关键词：矿井水高浊高铁锰改性

改性滤料处理高浊高铁锰矿井水效能和机制研究

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中国矿业大学学报 2009年第5期38卷 724-728页

作者：何绪文周波邵立南黄静华李焱中国矿业大学化学与环境工程学院北京100083 中国矿业大学机电与信息工程学院北京100083

针对常用滤料对锰的去除率不高和成熟期长的问题,研究开发了一种改性锰砂滤料,测定了改性滤料的比表面积和Zeta电位,并使用该滤料进行了高浊高铁锰矿井水净化实验.结果表明:该滤料具有稳定的处理效果,在24 h连续处理的条件下,污染物去... 详细信息

针对常用滤料对锰的去除率不高和成熟期长的问题,研究开发了一种改性锰砂滤料,测定了改性滤料的比表面积和Zeta电位,并使用该滤料进行了高浊高铁锰矿井水净化实验.结果表明:该滤料具有稳定的处理效果,在24 h连续处理的条件下,污染物去除率保持在95%以上.滤料改性后,其比表面积为8.849 m2/g,与未改性前相比明显增大,可以大量地吸附水中铁锰离子;被吸附的离子,通过改性形成的滤膜(***2O)催化氧化成为三价铁和二氧化锰而被去除;改性滤料表面Zeta电位为-14.5 mV,其静电排斥作用比石英砂和锰砂弱得多,对悬浮颗粒向滤料表面靠近的阻碍相对较小,使悬浮颗粒易于去除.

关键词：改性滤料高浊高铁锰矿井水效能机制

高浊高铁锰矿井水中污染物在采空区内的迁移扩散

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中国矿业大学学报 2009年第1期38卷 135-139页

作者：邵立南何绪文黄静华何咏李焱中国矿业大学化学与环境工程学院北京100083 中国矿业大学机电与信息工程学院北京100083

为揭示高浊高铁锰矿井水中污染物在采空区内的迁移扩散规律,以采空区充填物为载体,进行了相应的动力学参数和动态迁移实验.结果表明:采空区充填物的弥散系数和吸附常数分别为D=3.12 m2/d,Kd(Fe)=11.07 mL/g,Kd(Mn)=7.543 mL/g;在连续运... 详细信息

为揭示高浊高铁锰矿井水中污染物在采空区内的迁移扩散规律,以采空区充填物为载体,进行了相应的动力学参数和动态迁移实验.结果表明:采空区充填物的弥散系数和吸附常数分别为D=3.12 m2/d,Kd(Fe)=11.07 mL/g,Kd(Mn)=7.543 mL/g;在连续运行条件下,距进水口0.5 m处,铁的去除率大于95%,浊度的去除率接近90%,但锰的去除率仅为40%,说明利用采空区充填物除锰时容易达到吸附饱和.建立了铁、锰在采空区内的迁移扩散模型,最大的误差仅为8.44%.该模型可进行迁移扩散预测,为设计参数的确定提供基础.

关键词：采空区高浊高铁锰矿井水迁移扩散

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高浓度泥浆法处理矿山酸性废水机理

中国有色金属学报 2012年第4期22卷 1177-1183页

作者：杨晓松邵立南刘峰彪何绪文中国矿业大学化学与环境工程学院北京100083 北京矿冶研究总院北京100044

针对传统石灰法处理矿山酸性废水存在结垢严重和处理效果不稳定等问题,研究了高浓度泥浆法(HDS)替代工艺。结果表明,高浓度泥浆法(HDS)处理矿山酸性废水的主要机理如下:酸碱中和、金属离子沉淀及共沉淀作用;污泥回流使沉淀底泥晶体化、... 详细信息

针对传统石灰法处理矿山酸性废水存在结垢严重和处理效果不稳定等问题,研究了高浓度泥浆法(HDS)替代工艺。结果表明,高浓度泥浆法(HDS)处理矿山酸性废水的主要机理如下:酸碱中和、金属离子沉淀及共沉淀作用;污泥回流使沉淀底泥晶体化、粗颗粒化,加快了污泥沉降和分离的速度;沉淀底泥Zeta电位提高,易于与带负电位的硫酸钙接近并附着,晶核不断长大,可显著延缓设备和管路的结垢。该技术处理矿山酸性废水效果好,出水水质稳定达到GB 8978—1996《污水综合排放标准》中二级排放标准的要求。

关键词：高浓度泥浆法矿山酸性废水废水处理机理

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博弈论在夜间建筑施工噪声控制中的应用

辽宁工程技术大学学报（自然科学版） 2006年第2期25卷 223-225页

作者：刘志斌马元吉邵立南辽宁工程技术大学资源与环境工程学院辽宁阜新123000 阜新市金城建筑工程有限公司辽宁阜新123000

针对建筑工地的夜间施工噪声问题,建立了管理者与被管理者之间的监督博弈模型,并用博弈论分析了管理者与被管理者在检查中的行为特点及其关系,讨论了现有的控制方法。在此基础上确定了管理者对被管理者的监管力度,得出了监管力度和惩罚... 详细信息

针对建筑工地的夜间施工噪声问题,建立了管理者与被管理者之间的监督博弈模型,并用博弈论分析了管理者与被管理者在检查中的行为特点及其关系,讨论了现有的控制方法。在此基础上确定了管理者对被管理者的监管力度,得出了监管力度和惩罚尺度与检查概率和夜间施工概率的关系。通过讨论认为对小的施工单位的检查力度要加大,对违规单位的处罚力度要大,并应将部分罚款作为环境监管部门的奖金和补助。

关键词：博弈论建筑噪声噪声控制