检索结果-南通市图书馆

Amperometric Glucose Sensor Based on Batch CuO-Modified Screen-Printed Silver-Carbon Electrodes

在线全文

学校读者我要写书评

暂无评论

Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 35-41页

作者： XIA Haiyan huang Yin YANG Hua WANG Xintian SHI Jianping LIU Lixia fu Yacheng QUAN Changyun LI Suyuan Cofoe Medical Technology Company Limited Changsha 410000China Institute of New Energy Hexi UniversityZhangye 734000China Chemistry and Chemical Engineering Central South UniversityChangsha 410000China

CuO nanoparticles were successfully synthesized via a two-jet electrospun method,and then screen-printed on silver-carbon electrodes,forming CuO-modified Ag-C(CuO/Ag-C)disposable strip *** natural environment condition for glucose detection,the obtained CuO/Ag-C electrodes show a high sensitivity of 540 nA·mM^(-1)·cm^(-2),and a low limit of detection(0.68 mM)in a wide linear response range of 0.68 mM and 3 mM(signal/noise=3),*** addition,the CuO/Ag-C electrodes also exhibit excellent anti-interference,air stability and *** a result,the fabrication of CuO nanoparticles via an electrospun process and the technique of screen-printed electrodes are of great significance for glucose detection.

关键词： electrochemical sensor CuO nanoparticles glucose detection screen-printing technique

Graphene oxide wrapped-silica microspheres humidity sensor with fast response/recovery, high sensitivity, and selectivity for pig respiratory rate monitoring

在线全文

学校读者我要写书评

暂无评论

Science China Technological Sciences 2025年

作者： Jing LI Bo LI Ziqi LIAO Yunhui DUAN Xinglan fu Yuehua huang Guanglin LI College of Engineering and Technology Southwest University Modern Agricultural Equipment Research Institute Xihua University

Respiratory rate monitoring is of great significance in pig healthy breeding for the early detection and timely prevention of respiratory diseases. Humidity sensing is an important noncontact method to monitor pig respiration, which does not cause direct harm to pigs and meets the requirements of animal welfare. This paper proposes a humidity sensor based on graphene oxide wrapped-silica microspheres （GO@SiO2）. First, the sensor shows fast response/recovery performances of0.24/0.73 s. Experiments demonstrate that the wrapped microsphere structure can inhibit water aggregation. It is speculated that the wrapped microsphere structure can inhibit the stacking of GO, and its curved surface can promote the evaporation of water molecules. Second, the sensor shows a high sensitivity of 1.2%/%RH–4.2%/%RH in 11%–95%RH range. This can be attributed to different sensing mechanisms, from hopping transport to increasing ion transport, leading to a significant change in the sensor’s resistance. Third, the sensor shows selectivity to water molecules due to the hydrophilic characteristic of GO and the significant increase in conductivity by ion transport. Finally, the sensor is applied to realize the respiratory rate monitoring of adult pigs and piglets.

关键词：

Single-cell omics: experimental workflow, data analyses and applications

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu fu Lei Gu Xin shao Qinqin Wang Xin Dong Bin Duan Feiyang Xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

括约肌间入路内口挂线加脓腔对口引流术治疗马蹄形肛周脓肿24例

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

世界华人消化杂志 2016年第7期24卷 1128-1133页

作者：皇甫少华江滨丁义江丁曙晴尹鑫袁庆延南京中医药大学第三附属医院肛肠科全国中医肛肠医疗中心江苏省南京市210001 南京中医药大学江苏省南京市210001

目的:评价括约肌间入路内口挂线加脓腔对口引流术在马蹄形肛周脓肿治疗中的安全性和有效性.方法:回顾性分析2014-01/2014-12南京中医药大学第三附属医院全国肛肠医疗中心九病区24例行括约肌间入路内口挂线加脓腔对口引流术的肛周脓肿患... 详细信息

目的:评价括约肌间入路内口挂线加脓腔对口引流术在马蹄形肛周脓肿治疗中的安全性和有效性.方法:回顾性分析2014-01/2014-12南京中医药大学第三附属医院全国肛肠医疗中心九病区24例行括约肌间入路内口挂线加脓腔对口引流术的肛周脓肿患者的临床资料.以临床痊愈及随访后是否复发、平均住院时间、术后平均恢复工作时间、平均住院费用、Wexner肛门失禁评分为评价指标.结果:本组患者平均年龄38.42岁±9.59岁,平均随访10.08 mo±3.32 mo,17例(70.83%)痊愈,7例(29.17%)形成肛瘘.7例成瘘患者中,2例经结核效应T细胞斑点检测确诊为结核病,转外院行抗结核治疗,随访9 mo,1例痊愈,1例好转;其余5例经二期手术后4例痊愈,1例未愈.结论:括约肌间入路内口挂线加脓腔对口引流术具有安全性好、损伤小、恢复时间短、手术相关费用低、可重复性好等优点,具有良好的安全性及有效性,可较好地应用于马蹄形肛周脓肿.

关键词：括约肌间切开内口挂线对口引流马蹄形肛周脓肿疗效及安全性

Hsa-miR-214-3p inhibits breast cancer cell growth and improves the tumor immune microenvironment by downregulating B7H3

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Oncology Research 2025年第1期33卷 103-121页

作者： YAN LU KANG WANG YUANHONG PENG MENG CHEN LIN ZHONG LUJI huang fu CHENG XINDAN SHENG XIN YANG MANZHAO OUYANG GEORGE A.CALIN ZHIWEI HE China-America Cancer Research Institute Guangdong Medical UniversityDongguan523808China GCP Center Shunde HospitalSouthern Medical University(The First People’s Hospital of Shunde Foshan)Shunde528300China Department of Gastrointestinal Surgery Shunde HospitalSouthern Medical University(The First People’s Hospital of Shunde Foshan)Shunde528300China The Second School of Clinical Medicine Southern Medical UniversityGuangzhou510080China Department of Translational Molecular Pathology The University of Texas MD Anderson Cancer CenterHoustonTX 77030USA Department of Oncology Shunde HospitalSouthern Medical University(The First People’s Hospital of Shunde Foshan)Shunde528300China

Background:Immune checkpoint inhibitors play an important role in the treatment of solid tumors,but the currently used immune checkpoint inhibitors targeting programmed cell death-1(PD-1),programmed cell death ligand-1(PD-L1),and cytotoxic T-lymphocyte antigen-4(CTLA-4)show limited clinical efficacy in many breast cancers.B7H3 has been widely reported as an immunosuppressive molecule,but its immunological function in breast cancer patients remains ***:We analyzed the expression of B7H3 in breast cancer samples using data from the Cancer Genome Atlas Program(TCGA)and the Gene Expression Omnibus(GEO)*** were selected using the TarBase,miRTarBase,and miRBase *** regulatory role of the microRNA hsa-miR-214-3p on B7H3 was investigated through dual-luciferase reporter assays,which identified the specific action sites of *** expression levels of B7H3 and hsa-miR-214-3p in human breast cancer tissues and adjacent normal tissues were quantified using Western blotting and quantitative PCR(qPCR).In vitro experiments were performed to observe the effects of modulating the expression of B7H3 or hsa-miR-214-3p on breast cancer cell proliferation and ***,the regulatory impact of hsa-miR-214-3p on B7H3 was ***-linked immunosorbent assays(ELISA)and flow cytometry were employed to assess the effects of co-cultured breast cancer cells and normal human peripheral blood mononuclear cells(PBMCs)on immune cells and associated ***:In breast cancer tissues,the expression level of B7H3 is inversely correlated with that of hsa-miR-214-3p,as well as with the regulatory effects on breast cancercell ***-miR-214-3p was found to inhibit breast cancer cell growth by downregulating ***,our research identified,for the first time,two binding sites for hsa-miR-214-3p on the 3’UTR of B7H3,both of which exert similar effects ***-culture experiments revealed that hsamiR-214-3p

关键词： Breast cancer B7H3 Hsa-miR-214-3p Immunotherapy

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

共反射面元叠加技术的应用

新疆石油地质 2002年第3期23卷 217-218页

作者：易宗富邵雨黄毅蒋在超严泽泉周清华中国石油新疆油田分公司勘探开发研究院地球物理研究所新疆乌鲁木齐830011

共反射面元叠加(CRA)是一种压制随机噪声的去噪方法,该方法在叠加或偏移地震剖面上进行多个共中心点(二维)或共反射面元(三维)的反射地震记录的“同相叠加”,通过增加叠加的覆盖次数达到提高地震资料信噪比的目的。阐述了共反射面元叠... 详细信息

共反射面元叠加(CRA)是一种压制随机噪声的去噪方法,该方法在叠加或偏移地震剖面上进行多个共中心点(二维)或共反射面元(三维)的反射地震记录的“同相叠加”,通过增加叠加的覆盖次数达到提高地震资料信噪比的目的。阐述了共反射面元叠加的基本原理,并用理论模型和实际地震资料处理效果证实该方法是基本保幅的去随机噪声方法。就保幅能力而言,该方法优于其它压制随机噪声的方法。

关键词：共反射面元叠加随机噪声去噪信噪比保幅 CRA 地震资料

Hot deformation behavior and dynamic recrystallization of 2195 Al–Li alloy with various pre-precipitation microstructures

在线全文

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2024年第5期34卷 955-966页

作者： Rong fu Hongbang shao Yuanchun huang Yu Liu Hui Li School of Mechanical and Electrical Engineering Central South University State Key Laboratory of High Performance and Complex Manufacturing Central South University Light Alloy Research Institute Central South University

In this work, 2195 Al–Li alloys with various precipitation microstructures were obtained by homogenization treatment followed by air-cooling(AC), discontinuous cooling(DC), and furnace-cooling(FC), and then tested by hot compression at different temperatures. The results show that the flow stresses of all specimens decrease with temperature and the peak stresses of AC, DC and FC specimens decrease in order at the same temperature. For FC samples, coarse pre-precipitates diminish the deformation resistance due to the reduction of solid solution strengthening and precipitation strengthening. Dynamic softening at low temperatures is significantly greater than that at high temperatures, except for the anomalous softening of the FC specimen at 520°C due to stress release by cracks. At 370–420°C, the dynamic softening of the AC specimen is more significant than the other samples, resulting from dynamic precipitation and precipitate coarsening. furthermore, there is considerable dynamic recrystallization(DRX) in FC samples and not in AC and DC samples, although their precipitates coarsen to similar levels at low temperatures. This suggests that DRX is associated with the particle-stimulated nucleation mechanism by regularly arranged precipitates. The banded or regularly arranged precipitates divide the Al matrix into multiple confined units, hindering dislocations and(sub)grain boundary movement, and thus promoting the development of sub-grains and DRX.

关键词： Al–Cu–Li alloy Deformation behavior Particles stimulated nucleation Pre-precipitate Recrystallization

美国小学科学教育标准修订的内容、特点及启示

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

教学与管理 2025年第2期 73-76页

作者：张绍清付超然赣南师范大学教育科学学院江西赣州341000 赣南师范大学物理与电子信息学院江西赣州341000

美国的《新一代科学教育标准》代表了科学教育的创新进步,整合了前沿科研,并修正了旧标准的问题。通过分析《新一代科学教育标准》在教学素养、课程内容、教学方法和评估方面的创新,以及凸显科学学科的基础作用、学科工具的运用、学科... 详细信息

美国的《新一代科学教育标准》代表了科学教育的创新进步,整合了前沿科研,并修正了旧标准的问题。通过分析《新一代科学教育标准》在教学素养、课程内容、教学方法和评估方面的创新,以及凸显科学学科的基础作用、学科工具的运用、学科理念的革新和教育目标的重新定位等新特征,从科学教育理念、内容与方法方面为我国科学教育的发展提出建议。

关键词：美国小学科学教育科学课程教育标准

Diversity of Nitrogenase Systems in Diazotrophs

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2006年第7期48卷 745-755页

作者： Ying Zhao shao-Min Bian Hui-Na Zhou Ju-fu huang Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany the Chinese Academy of Sciences Beijing 100093 China School of Graduate the Chinese Academy of Sciences Beijing 100039 China

Nitrogenase is a metalloprotein complex that catalyses the reaction of biological nitrogen fixation. At least three genetically distinct nitrogenase systems have been confirmed in diazotrophs, namely Nil, Vnf, and Anf, in which the active-site central metals are Mo, V, and Fe, respectively. The present review summarizes progress on the genetic, structural, and functional investigations into the three nitrogenases and discusses the possibility of the existence of other novel nitrogenases.

关键词： diazotroph FeMo-cofactor and cofactors substituted for Mo nitrogenase system P-cluster.