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Influence on Biological Behavior of Colon Cancer Stem Cells after RNA Interfering CD133

Clinical oncology and cancer researeh 2010年第6期7卷 359-365页

作者： liang AI Mei liU Mi chen shao-lin li Teaching and Research Section of Radiation Medicine School of Basic Medical Science Chongqing Medical University Chongqing 400016. China.

OBJECTIVE To observe the effects expression and activation on biological cancer stem cells. of blocking CD133 gene characteristic of the colon METHODS CD133＋ colon cancer stem cells （CCSCs） were separated from EpCAMhigh CD44＋ CCSCs through fluorescenceactivated cell sorting （FACS）. The proliferation, the capability of spherical cell formation, neoplasia, and the expression of ABCG2 mRNA of CD133＋ CCSCs were observed after the CD133＋ CCSCs were infected with LV-CD133shRNA. CD133 negative cells were isolated from EpCAMhigh CD44＋ CCSCs with FACS, and the CD133 proteins in CD133- cells were detected with Western blot. RESULTS CD133＋ CCSCs were isolated from EpCAMhigh CD44＋ CCSCs using FACS, and they accounted for 89.2% in the stem cells. In the experimental group, after the CD133＋ CCSCs were knocked down by LV-CD133shRNA RNAi, the growth pattern of the cells in the stem cell culture changed into adherent growth from suspended growth, and couldn＇t generate spherical cells. Results of MTT assay showed that the CD133＋ CCSCs infected with LV-CD133shRNA grew slowly, compared to the cells in the control groups. There was a decrease in the cloning efficiency. The infected cells were transplanted into the BALB/c nude mice. During the observation, no neoplasia was found in the CD133＋ cells infected with LV-CD133shRNA. The level of ABCG2 mRNA expression was lowered greatly （P 〈 0.01）. CD133- cells were obtained from the EpCAMhigh CD44＋ CCSCs using FACS, in which the expression of CD133 protein was positive. CONCLUSION CD133 retains the biological characteristics of the colon cancer stem cells.

关键词： colonic neoplasms stem cells CD133 protein mouse RNA interference biological behavior.

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Constraints on holographic QCD phase transitions from PTA observations

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 71-78页

作者： Song He li li Sai Wang shao-Jiang Wang Center for Theoretical Physics and College of Physics Jilin University School of Physical Science and Technology Ningbo University Max Planck Institute for Gravitational Physics (Albert Einstein Institute) CAS Key Laboratory of Theoretical Physics Institute of Theoretical Physics Chinese Academy of Sciences School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences (UCAS) School of Physical Sciences University of Chinese Academy of Sciences (UCAS) Theoretical Physics Division Institute of High Energy Physics Chinese Academy of Sciences

The underlying physics of QCD phase transition in the early Universe remains largely unknown due to its strong-coupling nature during the quark-gluon plasma/hadron gas transition, yet a holographic model has been proposed to quantitatively fit the lattice QCD data while with its duration of the first-order phase transition(FoPT) left undetermined. At specific baryon chemical potential, the first-order QCD phase transition agrees with the observational constraint of baryon asymmetry. It, therefore, provides a scenario for phase transition gravitational waves(GWs) within the standard model of particle physics. If these background GWs could contribute dominantly to the recently claimed common-spectrum red noise from pulsar timing array(PTA) observations, the duration of this FoPT can be well constrained, and the associated primordial black holes are still allowed by current observations.

关键词： pulsar timing array QCD phase transition gravitational waves primordial black holes

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A Review on Thermal Management of li-ion Battery:from Small-Scale Battery Module to Large-Scale Electrochemical Energy Storage Power Station

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Journal of Thermal Science 2025年第1期34卷 1-23页

作者： chen Zhifeng JIA li YIN liaofei DANG Chao REN Honglei ZHANG Zhiqiang Beijing Key Laboratory of Flow and Heat Transfer of Phase Changing in Micro and Small Scale School of MechanicalElectronic and Control EngineeringBeijing Jiaotong University

li-ion battery is an essential component and energy storage unit for the evolution of electric vehicles and energy storage technology in the ***,in order to cope with the temperature sensitivity of li-ion battery and maintain li-ion battery safe operation,it is of great necessary to adopt an appropriate battery thermal management system(BTMS).In this paper,the current main BTM strategies and research hotspots were discussed from two aspects:small-scale battery module and large-scale electrochemical energy storage power station(EESPS).The practical application situation,advantages and disadvantages,and the future development trend of each heat dissipation method(air,liquid,PCM,heat pipe,hybrid cooling) were described in *** them,the air cooling and liquid cooling were reviewed in-depth based on the engineering *** PCM,heat pipe and hybrid cooling were reviewed extensively based on the latest *** research provides a comprehensive understanding for the BTMS in all scales.

关键词： battery thermal management cooling methods large-scale electrochemical energy storage power station small-scale battery module

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Expression analysis of two novel cotton 14-3-3 genes in root development and in response to salt stress

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Progress in Natural Science:Materials International 2009年第2期19卷 173-178页

作者： Xinzheng Wei1,Zeting Zhang1,Yang li,Xiulan Wang,Suqiang shao,liang chen,Xuebao li Hubei Key Laboratory of Genetic Regulation and Integrative Biology,College of life Sciences,Huazhong Normal University,Wuhan 430079,China Hubei Key Laboratory of Genetic Regulation and Integrative Biology College of Life Sciences Huazhong Normal University Wuhan 430079 China

14-3-3 proteins are phosphoserine-binding proteins that regulate the activities of a wide array of targets via direct protein-protein interactions,and may play an important role in response to biotic and abiotic stresses. In this study,two cDNAs (designated as Gh14-3-3b and Gh14-3-3c) encoding putative 14-3-3 proteins were isolated from cotton cDNA libraries. Gh14-3-3b gene encodes a pro-tein of 268 amino acids,while Gh14-3-3c gene encodes a protein of 261 amino acids. Real-time RT-PCR analysis revealed that both the Gh14-3-3b and Gh14-3-3c genes were preferentially expressed in roots. The transcript levels of both the genes were the highest in 3-day-old roots,and then dramatically decreased to relatively low levels in parallel with root development. In addition,the expression of these Gh14-3-3 genes in roots was significantly up regulated by salt treatments,suggesting that they may be involved in the signaling pathways in response to salt stress in cotton.

关键词： Cotton (Gossypium hirsutum) 14-3-3 genes Gene expression Root development Salt stress

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Cyano migration-mediated radical-polar crossover cyclopropanation

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Science China Chemistry 2025年第1期68卷 241-248页

作者： Ziqiang Wang Yasu chen Jie li chen Zhu Key Laboratory of Organic Synthesis of Jiangsu Province College of Chemistry Chemical Engineering and Materials ScienceSoochow University Frontiers Science Center for Transformative Molecules Zhangjiang Institute for Advanced Study and Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs Shanghai Jiao Tong University

We report a novel cyano migration-mediated radical-polar crossover process, whereby both the sulfonylcyanation of aliphatic alkenes and the formation of cyclopropanes are concurrently achieved. This transformation yields a diverse array of multisubstituted cyclopropanes under mild photocatalytic conditions. Mechanistic studies elucidate that the reaction proceeds via intramolecular nucleophilic substitution, leading to the formation of the cyclopropane derivatives.

关键词： cyclopropanation cyano migration radical-polar crossover radical reactions

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文化产业领域IP孵化与艺术生产商业模式创新

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艺术百家 2017年第4期33卷 94-99页

作者：陈少峰李源北京大学哲学系北京100871

近几年来,IP热在我国的文化产业领域愈演愈烈。IP必须与时俱进,才能始终保持对消费群体的吸引力,因此要持续推动IP的巡转孵化,如此才能实现IP的内在增值,使IP永葆青春。目前有两种商业模式可以推动IP的巡转孵化:一是通过系列微电影持续... 详细信息

近几年来,IP热在我国的文化产业领域愈演愈烈。IP必须与时俱进,才能始终保持对消费群体的吸引力,因此要持续推动IP的巡转孵化,如此才能实现IP的内在增值,使IP永葆青春。目前有两种商业模式可以推动IP的巡转孵化:一是通过系列微电影持续孵化IP,二是做文化电商,把内容植入和创意产品进行结合,即"内容+衍生品+电商"的文化产业模式。

关键词：文化产业 IP巡转孵化艺术生产商业模式微电影文化电商

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A Robust Acquisition Scheme for FH Signal in Adverse Environments

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Journal of Electronic Science and Technology of China 2009年第2期7卷 110-114页

作者： Ya-Ding chen shao-Qian li Yu-Fan cheng Gang Wu The authors are with National Key Lab. of Communication Universityof Electronic Science and Technology of China Chengdu 610054 China

Both partial-band jamming and multitone jamming have severe effect on the acquisition of frequency-hopping （FH） signal in adverse environments. In this paper, an anti-jamming FH signal acquisition scheme based on cognitive correlation process is proposed to boost the robustness of acquisition. The main idea of this scheme is to utilize a priori knowledge of FH speed and FH pattern to distinguish jamming signal from received signal. Furthermore, theoretic analysis on detection probability and false probability is given to demonstrate the robust performance of the FH signal acquisition method compared with conventional acquisition scheme without any prior information on FH speed and pattern in adverse environments.

关键词： Index Termsm-Acquisition anti-jamming detection,frequency hopping (FH).

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Investigation on Structures and Nonlinear Optical Properties of lithium-Atom Surface-Doping Super-short Carbon Nanotube

Investigation on Structures and Nonlinear Optical Properties...

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中国化学会第29届学术年会

作者： shao-chen li Guang-Tao Yu Wei chen Xu-Ri Huang The State Key Laboratory of Theoretical and Computational Chemistry Institute of Theoretical ChemistryJilin University

Under density functional theory method,the structures and nonlinear optical(NLO)properties of lin@cyclophenacene(n=1)species were investigated,where one to three li atoms are doped over the super-short carbon *** doped systems can exhibit the considerable adsorption energies(20.05.4 kcal/mol).Besides,when doping one to two alkali li atoms,the first hyperpolarizability of the super-short carbon nanotube can be effectively improved,where theβ0 value can be significantly increased in the range of 3.42×103.29×103 au,owing to the occurrence of charge ***,as increasing the number of the doped li atoms to three,theβ0 value can be further enhanced sharply,even to as large as 2.59×106 au,which can be attributed to the formation of excess ***,the distance between the doped li atoms can also have an important *** work can provide some valuable insights for designing the new high-performance carbon-based NLO materials.

关键词： Alkali metal atom Super-short carbon nanotube The first hyperpolarizabilities Charge transfer Excess electron

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Cell polarization in ischemic stroke: molecular mechanisms and advances

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Neural Regeneration Research 2025年第3期20卷 632-645页

作者： Yuanwei li Xiaoxiao Xu Xuan Wu Jiarui li Shiling chen Danyang chen Gaigai li Zhouping Tang Department of Neurology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Ischemic stroke is a cerebrovascular disease associated with high mortality and disability rates. Since the inflammation and immune response play a central role in driving ischemic damage, it becomes essential to modulate excessive inflammatory reactions to promote cell survival and facilitate tissue repair around the injury site. Various cell types are involved in the inflammatory response, including microglia, astrocytes, and neutrophils, each exhibiting distinct phenotypic profiles upon stimulation. They display either proinflammatory or anti-inflammatory states, a phenomenon known as ‘cell polarization.’ There are two cell polarization therapy strategies. The first involves inducing cells into a neuroprotective phenotype in vitro, then reintroducing them autologously. The second approach utilizes small molecular substances to directly affect cells in vivo. In this review, we elucidate the polarization dynamics of the three reactive cell populations(microglia, astrocytes, and neutrophils) in the context of ischemic stroke, and provide a comprehensive summary of the molecular mechanisms involved in their phenotypic switching. By unraveling the complexity of cell polarization, we hope to offer insights for future research on neuroinflammation and novel therapeutic strategies for ischemic stroke.

关键词： astrocyte polarization immune regulation inflammation ischemic injury microglia polarization neutrophil polarization signaling pathways stroke

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Targeting cholesterol trafficking to mitigate axonal degeneration in hereditary spastic paraplegia

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Neural Regeneration Research 2025年第5期20卷 1397-1398页

作者： Zhenyu chen Xue-Jun li Department of Biomedical Sciences University of Illinois College of Medicine RockfordRockfordILUSA Department of Bioengineering University of Illinois at ChicagoChicagoILUSA

Axonal degeneration underlies many debilitating diseases including hereditary spastic paraplegia(HSP),a genetically and clinically diverse group of disorders characterized by spasticity and weakness of the lower *** is one significant cause of chronic neurodisability due to the lack of effective treatments and a wide range of onset ages from early childhood to 70 years.

关键词： degeneration diseases hereditary

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