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QT离散度与恶性室性心律失常的关系简析

实用医技杂志 2004年第7A期11卷 1178-1179页

作者：安毅山西医科大学第二医院山西太原030001

目的 :探讨 QT离散度 (QTd)与恶性室性心律失常的关系。方法 :测量 89例患者同步 12导联心电图。结果 :在恶性室性心律失常组 (42例 ) QTd为 (92± 31) ms,非恶性室性心律失常组 (47例 )为 (6 2± 19) ms,两组比较差异有极显著性 (P<0 .... 详细信息

目的 :探讨 QT离散度 (QTd)与恶性室性心律失常的关系。方法 :测量 89例患者同步 12导联心电图。结果 :在恶性室性心律失常组 (42例 ) QTd为 (92± 31) ms,非恶性室性心律失常组 (47例 )为 (6 2± 19) ms,两组比较差异有极显著性 (P<0 .0 1) ,随 QTd增大 ,恶性室性心律失常发生显著增加 (高 )。结论 :QTd是恶性室性心律失常监测的重要指标。

关键词： QT离散度恶性室性心律失常 QTd 心脏病

Inhibition Proliferation Properties of 15-PGDH Gene in Human Colorectal Cancer

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Inhibition Proliferation Properties of 15-PGDH Gene in Human...

2008年全国生物化学与分子生物学学术大会

作者： Niu-liang Cheng Mei-ning Li Yan Feng Yue-hong Zhang Dong Zhang (Department of Biochemistry and Molecular Biology,shanxi medical university,Taiyuan 030001)

Colorectal cancer(CRC)is one of the most common gastrointestinal malignancies that severely threaten the health of *** is of great significance to study the underlying mechanisms of CRC for its prevention and *** first and key enzyme involved in prostaglandin catabolism is NAD-linked 15-Hydroxyprostaglandin dehydrogenase(PGDH),which is reported as a new tumor suppressor *** is widely distributed in

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The expression of TrkA in brain original neural stem cells of embryonic rat

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The expression of TrkA in brain original neural stem cells o...

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Jin-Li WANG,Chun-Fang WANG,Peng-Fei LI,Hong-En WEI,Yao-Jun SUO,Xiao-Xia WANG Department of Molecular Biology,medical Experiment Center,shanxi medical university,Taiyuan 030001

＜正＞Objective To investigate the expression of Tyrosine kinase A（TrkA） in cerebral cortex neural stem cells of embryonic rats,the research tries to find functions of TrkA and demonstrate the possible mechanism of neural stem cells directional differentiation during the development of neural *** We take pregnant 13,15,17,19,21d（E13, E15,E17,E19,E21） and postnatal 1 d（P1） Wistar rats to make paraffin *** use Nestin to identify neural stem cells and then test the expression of Nestin and TrkA respectively and their coexpression using immunofluorescence（IF） double staining in above different *** calculate the average positive rate,demonstrated as±SD and progress statistic *** TrkA distribute in rat brain tissue extensively during embryonic stage;the expression has following variance along with different developmental period:in cerebral cortices,at E13 TrkA only express small amounts, step up at E15 then up to peak at E17,gradually decrease from E19 to E21 and increase a little at P1 in cerebral *** expression regularity of Nestin is similar to TrkA,low expresses at E13,increases and reaches peak at E15,then decreases at E17 and E19,but slightly heightens at *** tendency of coexpression is that they start expression at E13,arrive peak time at E15,afterward fall ***（1） TrkA expresses extensively in cerebral cortices during the rat embryonic development and reaches peak at E17,which may means rat brain development requires maximum NGF at E17.（2） The diverse expression of Nestin demonstrates that we may get more neural stem cells from E15 brain.（3） The abundance expression of TrkA in neural stem cells indicates that we can select E15 rat as the source of neural stem cells,which induced by NGF to differentiated into specific neurons.（4） The regularity of coexpression reveals the mechanism of the neural development and the biological character of neural stem cells.

关键词： neural stem cells TrkA Nestin coexpression rat

Construction of HPV-16-L1,HSV-gD Vaccines with their protein expressions in DH5 α and possibly DCs present HPV-16-L1 protein to lymphocytes

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Construction of HPV-16-L1,HSV-gD Vaccines with their protein...

2012全国临床微生物与感染免疫学术研讨会

作者： Yan Bing~(1,2),Meng Xia~1,Jiang Yuanhong~(1,3),Wang Jianyu~1,Fan Fan Xiaoguang~1 1.Department of Microbiology and Immunology,2 Department of Electron Microscope,shanxi medical university 2.College of Clinical Medicine at five years length 2006,West China Center of medical Science,Sichuan university 3.Department of Electron Microscope,shanxi medical university

Objective It is well known that cervical cancer has close relationship with HPV and HSV and the investigation showed that 50%cervical cancer patients had been infected by HPV 16,of which L1 is the major capsid protein, an antigen can stimulate lymphocyte in the host to produce antibody to neutralize the ***Ⅱis the cofactor in the processing and development of cervical *** order to obtain the recombinant protein of pBV220- HPV16L1 and pBV220-HSVgD,it’s necessary to induce their expression in ***5αand purify the acquired *** use these two sets of recombinant protein to prepare for the polyclonal antibodies by immunized New Zealand White rabbits. Methods The source of the DNA were extracted and amplified by PCR reaction using sense primer and antisense primer according to the HPV type 16-L1 gene is the tissue of patients,whose diagnose not only confirmed clinically,but also reassured by their tissue sample ***,gD gene was also amplified from HSV as well. The *** DH5αwith the recombinant plasmids express pBV220-HPVL1 and pBV220-HSVgD respectively in the form of inclusion body after induced at 42℃for 5 hours or 6 hours *** obtained protein can be detected by *** cracking the bacteria with sonar,we got the inclusion bodies that contain the target *** they were washed in solution that contains 2 mol/L urea as final concentration for acquire the recombinant proteins at high purity,following by the Branford method for *** we immunize the rabbit with these proteins by injecting 1 mg/kg for the first inoculation and 0.5 mg/kg for the rest 6 shoots or till the titer and specificity of the HPVL1 and HSV-gD antibodies were high enough to be detected by agarose double immune *** the blood of the rabbits from their hearts were collected and sera were kept in -20℃environment for the western blot. Recombinant HPV16-L1 protein was presented by Dendritic Cells(DCs) to lymph

关键词： Constructs of HSV -gD and HPV -16-L1 Recombinant protein expression Polyclonal antibody DCs present HPV-16- L1 protein to lymphocytes

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糖尿病病人的行为干预治疗

护理研究 2003年第12期17卷 695-696页

作者：郝牡丹山西医科大学第二医院 030001

糖尿病是终身性疾病,其发病率随着经济条件的改善而不断上升.对糖尿病的治疗是一个漫长而持久的过程.糖尿病的治疗需要饮食、运动、药物、自我监测、健康教育这5个方面同时进行.

关键词：糖尿病病人尿糖饮食治疗自我监测行为干预治疗饮食控制血糖

20S Proteasome is essential for neural stem cells maintenance in SVZ

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20S Proteasome is essential for neural stem cells maintenanc...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Zhong-Nian XIA,Wei-Guo ZHANG,Yun-He ZHAO,Qian ZHU~1,Li LU~* Department of Anatomy,shanxi medical university,Taiyuan 030001,China

＜正＞Objective To examine 20S proteasome activity and its expression in the subventricular zone（SVZ） of mice at different ages,then investigating the effects of proteasome inhibitor MG132 on the viability and proliferation potential of neural stem cells（NSCs）,thus to establish the relationships between 20S proteasome activity and NSCs *** work aimed to unveil the underlying mechanism for NSCs dysfunction in aging brain,so as to develop a new treatment stratedgy for age-related neurodegenerative *** In the present study,immunostaining was used to check the expression of 20S proteasome in embryonic and adult mice *** test whether 20S proteasome deficiency and expression level display in an age-dependent manner in SVZ,proteasome peptidase assay and Western Blot assay were performed on mice at embryonic（embryonic day 14）,neonatal（postnatal day 0）,adult（postnatal day 90）,aged（postnatal day 540） ***,proteasome inhibitor MG132 was injected into lateral ventricles of adult mice to examine its effects on NSCs proliferation by BrdU labeling at day 3 postinjection. Furthermore,in vitro analyses were used to determine the effect of MG132 on NSCs viability from SVZ by BrdU incorporation and Cell Counting Kit-8（CCK-8） ***（1） 20S proteasome was collocalized with NSCs marker nestin in SVZ of embryonic and aged mouse,20S proteasome-positive cells were distributed along the ventricular wall.（2） Age-dependent decline in the activity and expression of 20S proteasome were found in SVZ,the 20S proteasome activity was significantly reduced 3～4 times in aged mice verse embryonic or neonatal mice（P＜0.05）. Moreover,the decreased numbers of BrdU-positive cells were founded in SVZ of aged *** observations indicated that 20S proteasome is associated with neural stem cells maintenance.（3） After intracerebroventricular injection of MG132,the numbers of BrdU-positive cells were significantly decreased to 21+4 at post-injection day 3 compared t

关键词： 20S proteasome neural stem cells MG132 maintenance

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利用因特网查询医学信息资源的方法和技巧

实用医技杂志 2004年第12A期11卷 2600-2601页

作者：张素萍山西医科大学汾阳学院山西汾阳032200

本文结合作者的实践经验 ,介绍了一些在因特网上查询和获取医学信息资源的方法和技巧。

关键词：因特网医学信息检索

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民族传统养生与传统文化

搏击.武术科学 2007年第7期4卷 84-85页

作者：安彦山西医科大学体育部山西太原030001

民族传统养生是中华民族的瑰宝,它包涵了数千年来先人从人生实践中体察到的人体生命真谛和祛病养生之道。文章从文化、哲学、医学等方面对民族传统养生进行了分析研究,力图从多个视角审视传统养生,使我们能更好的认识传统养生,促进提高... 详细信息

民族传统养生是中华民族的瑰宝,它包涵了数千年来先人从人生实践中体察到的人体生命真谛和祛病养生之道。文章从文化、哲学、医学等方面对民族传统养生进行了分析研究,力图从多个视角审视传统养生,使我们能更好的认识传统养生,促进提高人们的健康水平。

关键词：传统养生哲学医学文化

胚胎及新生大鼠脑组织神经干细胞巢蛋白表达的发育改变

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解剖学杂志 2004年第3期27卷 253-255页

作者：崔爱玲王春芳杨涛窦岩山西医科大学医学实验中心

目的 :检测不同发育时期脑组织神经干细胞巢蛋白 (nestin)的表达和发育变化规律 ,为分离、富集和增殖神经干细胞提供实验依据。方法 :将Wistar大鼠按胎龄和日龄分组 ,剥离脑组织 ,制成单细胞悬液 ,经间接荧光法标记nestin抗原 ,用流式... 详细信息

目的 :检测不同发育时期脑组织神经干细胞巢蛋白 (nestin)的表达和发育变化规律 ,为分离、富集和增殖神经干细胞提供实验依据。方法 :将Wistar大鼠按胎龄和日龄分组 ,剥离脑组织 ,制成单细胞悬液 ,经间接荧光法标记nestin抗原 ,用流式细胞术检测并分析。结果 :E1 3至P90整个发育过程中都有nestin抗原的表达 ;从E1 3到E1 9表达逐渐升高 ,E1 9表达水平最高 ;出生后表达急剧下降 ,出生后 7d便接近于出生后 90d(成年鼠 )表达水平。结论 :胚胎发育近中期后 ,脑神经干细胞nestin表达水平随胎龄逐渐升高 ,E1 9达到高峰 ,出生后表达迅速下降。

关键词：胚胎新生大鼠脑组织神经干细胞细胞巢蛋白表达流式细胞术神经发育