检索结果-南通市图书馆

同方期刊数据库

A systematic study of the determinants of protein abundance memory in cell lineage

在线全文

学校读者我要写书评

暂无评论

Science Bulletin 2018年第16期63卷 1051-1058页

作者： Zongmao Gao Haoyuan Sun shanshan qin Xiaojing Yang Chao Tang Center for Quantitative Biology Peking University Peking-Tsinghua Center for Life Sciences Peking University School of Physics Peking University

Proteins are essential players of life activities, lntraceUular protein levels directly affect cellular functions and cell fate. Upon cell division, the proteins in the mother cell are inherited by the daughters. However, what factors and by how much they affect this epigenetic inheritance of protein abundance remains unclear. Using both computational and experimental approaches, we systematically investigated this problem. We derived an analytical expression for the dependence of protein inheritance on various factors and showed that it agreed with numerical simulations of protein production and experimental results. Our work provides a framework for quantitative studies of protein inheritance and for the potential application of protein memory manipulation.

关键词： Protein abundance memory Intrinsic noise Extrinsic noise Epigenetic inheritance Cell lineage

Fiber-Shaped Triboiontronic Electrochemical Transistor

在线全文

学校读者我要写书评

暂无评论

Research 2021年第1期2021卷 1245-1254页

作者： Jinran Yu shanshan qin Huai Zhang Yichen Wei Xiaoxiao Zhu Ya Yang Qijun Sun Beijing Institute of Nanoenergy and Nanosystems Chinese Academy of SciencesBeijing 101400China School of Nanoscience and Technology University of Chinese Academy of SciencesBeijing 100049China Department of Materials Science WW-4 LKOUniversity of Erlangen-NurembergMartensstrasse 791058 ErlangenGermany Center on Nanoenergy Research School of Physical Science and TechnologyGuangxi UniversityNanning 530004China Beijing Institute of Fashion Technology Beijing 100029China

Contact electrification-activated triboelectric potential offers an efficient route to tuning the transport properties in semiconductor devices through electrolyte dielectrics,i.e.,*** electrochemical transistors(OECTs)make more effective use of ion injection in the electrolyte dielectrics by changing the doping state of the semiconductor ***,the mainstream flexible/wearable electronics and OECT-based devices are usually modulated by electrical signals and constructed in conventional geometry,which lack direct and efficient interaction between the external environment and functional electronic ***,we demonstrate a fiber-shaped triboiontronic electrochemical transistor with good electrical performances,including a current on/off ratio as high as≈1286 with off-current at~nA level,the average threshold displacements(D_(th))of 0.3 mm,the subthreshold swing corresponding to displacement(SS_(D))at 1.6 mm/dec,and excellent flexibility and *** proposed triboiontronic electrochemical transistor has great potential to be used in flexible,functional,and smart self-powered electronic textile.

关键词： transistor tuning durability

Photolithography-free fabrication of photoresist-mold for rapid prototyping of microfluidic PDMS devices

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2022年第2期33卷 987-989页

作者： shanshan qin Gaozhi Ou Biao Wang Zheyu Li Rui Hu Ying Li Yunhuang Yang Key Laboratory of Magnetic Resonance in Biological Systems State Key Laboratory of Magnetic Resonance and Atomic and Molecular PhysicsNational Center for Magnetic Resonance in WuhanWuhan Institute of Physics and MathematicsInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of Sciences-Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and TechnologyWuhan 430071China University of Chinese Academy of Sciences Beijing 10049China School of Physical Education China University of GeosciencesWuhan 430074China

Traditional soft lithography based PDMS device fabrication requires complex procedures carried out in a clean room. Herein, we report a photolithography-free method that rapidly produces PDMS devices in 30 min. By using a laser cutter to ablate a tape, a male photoresist mold can be obtained within 5 min by a simple heating-step, which offers significant superiority over currently used photolithographybased method. Since it requires minimal energy to cut the tape, our fabrication strategy shows good resolution(~ 100 μm) and high throughput. Furthermore, the micro-mold height can be easily controlled by changing the tape types and layers. As a proof-of-concept, we demonstrated that the fabricated PDMS devices are compatible with biochemical reactions such as quenching reaction of KI to fluorescein and cell culture/staining. Collectively, our strategy shows advantages of low input, simple operation procedure and short fabrication time, therefore we believe this photolithography-free method could serve as a promising way for rapid prototyping of PDMS devices and be widely used in general biochemical laboratories.

关键词： Microfluidics PDMS Photolithography-free Rapid prototyping Laser cutting Soft lithography

DOW CORNING 1-2577 Conformal Coating as an efficient diffusion material for cathode in the microbial fuel cell

在线全文

学校读者我要写书评

暂无评论

Frontiers of Environmental Science & Engineering 2013年第4期7卷 526-530页

作者： Yanping HO~ Haiping LUO Guangli LIU Renduo ZHANG Yong LUO Bangyu qin shanshan CHEN The Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology School of Environmental Science and Engineering Sun Yat-Sen University Guangzhou 510275 China

In this study, DOW CORNING 1-2577 Con- formal Coating was proposed for the cathode diffusion layer of the microbial fuel cell （MFC）. In MFCs, stainless steel mesh cathodes using DOW CORN1NG 1-2577 Conformal Coating/carbon as the diffusion layer and two poly （dimethylsiloxane）（PDMS）/carbon diffusion layers and carbon cloth cathode with four poly （tetrafluoroethy- lene）（PTFE） diffusion layers were constructed for comparison. Under the same operational condition, the MFCs with the DOW CORNING 1-2577 Conformal Coating/carbon diffusion layer produced the maximum power density of 1585q-52mW-m2, compared with those using poly （tetrafluoroethylene）（PTFE） diffusion layers （1421 ~45 mW. m-2） and poly （dimethylsiloxane）（PDMS）/carbon diffusion layers （13534-49 mW. m-E）. The DOW CORNING 1-2577 Conformal Coating could be an alternative for the diffusion layer construction in the MFC due to its remarkable performance and much simple construction procedure.

关键词： microbial fuel cell diffusion layer poweIdensity DOW CORN1NG1-2577 Conformal Coating

A two-dimensional subthreshold current model for strained-Si MOSFET

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2011年第12期54卷 2181-2185页

作者： qin shanshan ZHANG HeMing HU HuiYong WANG GuanYu WANG XiaoYan QU JiangTao XU XiaoBo Key Laboratory of Ministry of Education for Wide Band-Gap Semiconductor Materials and Devices School of Microelectronics Xidian University Xi＇an 710071 China

An analytical model for the subthreshold current of a strained-Si metal-oxide-semiconductor field-effect transistor (MOSFET) is developed by solving the two-dimensional (2D) Poisson equation and the conventional drift-diffusion theory. Model verification is carried out using the 2D device simulator ISE. Good agreement is obtained between the model's calculations and the simulated results. By analyzing the model, the dependence of current on the strained-Si layer strain, doping concentration, source/drain junction depths and substrate voltage is studied. This subthreshold current model provides valuable information for strained-Si MOSFET design.

关键词： strained-Si MOSFET surface voltage subthreshold current

Visually Intracellular Detection of Telomerase Activity Based on DNA Strand Displacement Reaction and Gold Nanoparticle Labeling

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2022年第6期40卷 693-698页

作者： Yajun Zhang Shen Yan Zhaoxin Chen Xin Jiang Shuang Rao Zhuoran Jiang shanshan qin Xiang Zhou Yuhao Du College of Chemistry and Molecular Sciences Key Laboratory of Biomedical Polymers of Ministry of EducationWuhan UniversityWuhanHubei 430072China

Telomerase,which is regarded as a common biomarker for early cancer diagnostics and a potential target for clinical therapies,has attracted considerable interests concerning its detection and ***,we propose a sensitive method by designing a gold nanoparticle(AuNP)probe for visually intracellular detection of telomerase *** AuNPs were functionalized with a telomer-ase substrate primer(SH-prime).A 6-carboxy-fluorescein(FAM)modified strand(FAM-probe)was attached to the surface of AuNP through its complementary stand(SH-attach).In the absence of telomerase,the fluorescence resonance energy transfer(FRET)from FAM to AuNPs results in efficient fluorescence *** the presence of telomerase,SH-primers on AuNPs were extended with the repeat units(TTAGGGG)*** extension sequence triggered the strand displacement of FAM-probe to restore the fluorescence *** is worth mentioning that the proposed strategy does not need to design complex hairpin structure and allows the meas-urement of telomerase in crude cell extracts down to 0.5 HeLa cells/μL in 2 *** addition,the present sensing platform can be ap-plied to the visually intracellular detection of telomerase activity in living cells.

关键词： Telomerase Nanoparticles DNA strand displacement Intracellular FRET

The development of an iridium(Ⅲ) complex functionalized G-quadruplex probe for the stability of G-quadruplex and lifetime image in cytoplasm

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2023年第2期34卷 397-402页

作者： Shaokang Jia Wenjin Wang shanshan qin Shengjie Xie Lisi Zhan Qi Wei Ziang Lu Xiaolu Zhou Cong Chen Kun Chen Shen Yan Caiping Tan Zongwan Mao Xiang Zhou College of Chemistry and Molecular Sciences Key Laboratory of Biomedical Polymers-Ministry of EducationWuhan UniversityWuhan 430072China MOE Key Laboratory of Bioinorganic and Synthetic Chemistry School of ChemistrySun Yat-sen UniversityGuangzhou 510275China

G-quadruplex(G4) is widely known as a non-classical secondary structure of nucleic acid. With the indepth study of G4, it is an urgent need for a phosphorescent probe with a high G4 binding ability to evaluate the level of G4 in the cytoplasm. Thus, this study designed and synthesized Ir-PDP where an Ir(Ⅲ)complex was used as a phosphorescent emitter. Meanwhile, two installed PDPs(pyridostatin derivatives)were used to improve the combination ability with G4 and reduced the cytotoxicity of the Ir(Ⅲ) *** with other nucleic acid secondary structures, Ir-PDP produced a higher phosphorescence lifetime after interacting with G4. Ir-PDP was distributed in the cytoplasm of living cells, and two-photon phosphorescence lifetime imaging can detect the binding events of the probe in the cytoplasm. The addition of G4 binder PDS significantly regulated cytoplasmic phosphorescence lifetime. The project explored a new sensing pathway to observe the binding manners of probes in the cytoplasm through the phosphorescence lifetime of probes.

关键词： G-quadruplex Pyridostatin derivatives Iridium(Ⅲ)complex Phosphorescent probe Phosphorescence lifetime Cell imaging

Overexpression of CIP2A is associated with poor prognosis in multiple myeloma

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2017年第1期2卷 201-210页

作者： Xuewen Liu Wei Cao shanshan qin Te Zhang Junnian Zheng Ying Dong Pinghong Ming Qian Cheng Zheng Lu Yang Guo Baofu Zhang Ying Liu Laboratory of Molecular Target Therapy of Cancer Institute of Basic Medical SciencesHubei University of MedicineShiyanChina MOE Key Laboratory of Industrial Fermentation Microbiology College of BiotechnologyTianjin University of Science and TechnologyTianjinChina Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy Cancer InstituteXuzhou Medical UniversityXuzhouChina Department of Oncology The Second Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhouChina Department of Pathology Zhuhai Hospital Affiliated with Jinan UniversityZhuhaiChina

Cancerous inhibitor of protein phosphatase 2A(CIP2A),an endogenous protein phosphatase 2A(PP2A)inhibitor,has been identified as an oncoprotein in promoting cancer initiation and progression of several types of ***,the expression and the role played by CIP2A in the pathogenesis of multiple myeloma(MM)remain *** this study,we showed that CIP2A was overexpressed in human MM cell lines and MM patients’bone marrow *** analysis showed that CIP2A expression was significantly correlated with clinical stage and percent of plasma cells in bone ***–Meier analysis revealed that patients with high CIP2A expression presented with poorer overall survival rates than those with low CIP2A ***,CIP2A knockdown in MM cells resulted in attenuated proliferative *** addition,CIP2A depletion sensitizes dexamethasone(Dex)-resistant cells to *** effect of CIP2A on proliferation and Dex therapy was mediated by the inhibition of PP2A,which in turn activated *** vivo studies confirmed that CIP2A regulated MM tumorigenesis and the phosphorylation of *** together,our results suggest that CIP2A oncoprotein plays an important role in MM progression and could serve as a prognosis marker and a novel therapeutic target for the treatment of patients with MM.

关键词： CIP2A expression. prognosis

G protein-coupled receptors: structure- and function-based drug discovery

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2021年第2期6卷 297-323页

作者： Dehua Yang qingtong Zhou Viktorija Labroska shanshan qin Sanaz Darbalaei Yiran Wu Elita Yuliantie Linshan Xie Houchao Tao Jianjun Cheng qing Liu Suwen Zhao Wenqing Shui Yi Jjiang Ming-Wei Wang The National Center for Drug Screening Shanghai Institute of Materia MedicaChinese Academy of Sciences201203 ShanghaiChina The CAS Key Laboratory of Receptor Research Shanghai Institute of Materia MedicaChinese Academy of Sciences201203 ShanghaiChina School of Basic Medical Sciences Fudan University200032 ShanghaiChina University of Chinese Academy of Sciences 00049 BeijingChina iHuman Institute ShanghaiTech University201210 ShanghaiChina School of Life Science and Technology ShanghaiTech University201210 ShanghalChina School of Pharmacy Fudan University201203 ShanghaiChina

As one of the most successful therapeutic target families,G protein-coupled receptors(GPCRs)have experienced a transformation from random ligand screening to knowledge-driven drug *** are eye-witnessing tremendous progresses made recently in the understanding of their structure-function relationships that facilitated drug development at an unprecedented *** article intends to provide a comprehensive overview of this important field to a broader readership that shares some common interests in drug discovery.

关键词： drug function structure