检索结果-南通市图书馆

Virologica Sinica 2022年第3期37卷 380-389页

作者： Hang Yang Huijun Yuan Xiaohui Zhao Meng Xun shangrui guo Nan Wang Bing Liu Hongliang Wang Department of Pathogen Biology and Immunology School of Basic Medical SciencesXi'an Jiaotong University Health Science CenterXi'an710061China School of Pharmacy Xi’an Jiaotong University Health Science CenterXi'an710061China BioBank The First Affiliated Hospital of Xi'an Jiaotong UniversityXi'an710061China Department of Life Sciences Faculty of Natural SciencesImperial College LondonLondonSW72AZUnited Kingdom Key Laboratory of Environment and Genes Related to Diseases Xi'an Jiaotong UniversityXi'an710061China

The recent COVID-19 pandemic poses a global health emergency.Cellular entry of the causative agent SARS-CoV-2 is mediated by its spike protein interacting with cellular receptor-human angiotensin converting enzyme 2(ACE2).Here,by using lentivirus based pseudotypes bearing spike protein,we demonstrated that entry of SARS-CoV-2 into host cells was dependent on clathrin-mediated endocytosis,and phosphoinositides played essential roles during this process.In addition,we showed that the intracellular domain and the catalytic activity of ACE2 were not required for efficient virus entry.Finally,we showed that the current predominant Delta variant,although with high infectivity and high syncytium formation,also entered cells through clathrin-mediated endocytosis.These results provide new insights into SARS-CoV-2 cellular entry and present proof of principle that targeting viral entry could be an effective way to treat different variant infections.

关键词： SARS-CoV-2 Endocytosis Phosphoinositides Angiotensin converting enzyme 2(ACE2) Syncytium

Construction of coxsackievirus B5 viruses with luciferase reporters and their applications in vitro and in vivo

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2023年第4期38卷 549-558页

作者： shangrui guo Meng Xun Tingting Fan Xinyu Li Haoyan Yao Xiaozhen Li Bo Wu Hang Yang Chaofeng Ma Hongliang Wang Department of Pathogen Biology and Immunology Xi’an Jiaotong University Health Science CenterXi’an710061China Department of Gynecology and Obstetrics The First Affiliated Hospital of Xi'an Jiaotong UniversityXi’an710061China Department of Viral Diseases Laboratory Xi’an Center for Disease Control and PreventionXi’an710061China

Coxsackievirus belongs to the Picornaviridae family and is one of the major pathogens that cause hand,foot and mouth disease(HFMD)in infants and children with potential serious complications and even deaths.The pathogenesis of this virus is not fully elucidated and no vaccine or antiviral drug has been approved.In this study,a full-length infectious cDNA clone of coxsackievirus B5 virus was assembled and the recombinant virus displayed similar growth kinetics and ability to cause cytopathic effects as the parental virus.Luciferase reporter was then incorporated to generate both full-length and subgenomic replicon(SGR)reporter viruses.The full-length reporter virus is suitable for high-throughput antiviral screening,while the SGR is a useful tool to study viral-host interactions.More importantly,the full-length reporter virus has also been shown to infect the suckling mouse model and the reporter gene could be detected using an in vivo imaging system,thus providing a powerful tool to track viruses in vivo.In summary,we have generated coxsackievirus B5 reporter viruses and provided unique tools for studying virus-host interactions in vitro and in vivo as well as for high-throughput screenings(HTS)to identify novel antivirals.

关键词： Coxsackievirus Luciferase High-throughput screening Mouse model

Microwave-assisted Synthesis of Al_(4)SiC_(4) and Its Effect on Properties of MgO-C Refractories

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

China's Refractories 2024年第1期33卷 14-17页

作者： WANG Li LIU Shijie WEI Haoyu guo Yanyan GENG shangrui YAN Miaoxin QIN Feng guo Yusen Ma Juanjuan DONG Binbin Henan Key Laboratory of Green Building Materials Manufacturing and Intelligent Equipemnt Luoyang Institute of Science and TechnologyLuoyang 471023China

Al_(4)SiC_(4) was synthesized from Al powder, silicon carbide, and graphite by microwave sintering, and characterized by XRD and SEM. Then the synthesized material was added to the magnesia carbon refractory brick to study its effect on the oxidation resistance, apparent porosity, bulk density, elastic modulus, and modulus of rupture. It is found that Al_(4)SiC_(4) can be synthesized by microwave sintering at 1 300 ℃ and the addition of Al_(4)SiC_(4)-containing material as an antioxidant can enhance the oxidation resistance of the magnesia carbon refractory brick.

关键词： microwave-assisted synthesis Al_(4)SiC_(4) MgO-C refractories oxidation resistance

Aluminum and Activated Alumina Powder Additions on Microwave Synthesis of Al_(4)SiC_(4)

在线全文

学校读者我要写书评

暂无评论

China's Refractories 2024年第1期33卷 44-48页

作者： WANG Li LIU Shijie WEI Haoyu MA Juanjuan DONG Binbin GENG shangrui YAN Miaoxin QIN Feng guo Yusen Henan Key Laboratory of Green Building Materials Manufacturing and Intelligent Equipemnt Luoyang Institute of Science and TechnologyLuoyang 471023China

Aiming at improving the properties of magnesia carbon materials,silicon aluminum carbide(Al_(4)SiC_(4))containing materials were prepared using industrial aluminum powder,silicon carbide powder,and graphite as raw materials,and activated alumina powder as an additive,mixing thoroughly,pressing into cylinders and then firing at 1200℃for 30 min in a carbon embedded atmosphere by the microwave method.The effects of the aluminum powder addition(20%and 24%,by mass)and activated alumina powder addition(0,3%,5%and 7%,by mass)on the microwave synthesis of Al_(4)SiC_(4) as well as the effect of the obtained Al_(4)SiC_(4) containing material on the properties of magnesia carbon bricks were studied.The results show that:compared with the samples with 20%aluminum powder,those with 24%aluminum powder generate more Al_(4)SiC_(4).With the activated alumina powder addition increasing from 0 to 7%,the amount of Al_(4)SiC_(4) generated increases first and then decreases.Compared with the sample without activated alumina powder,the samples with activated alumina powder show lower bulk density and higher apparent porosity.With the activated alumina powder addition increasing from 3%to 7%,the bulk density of the samples increases first and then decreases,while the apparent porosity of the samples shows an opposite trend.The optimal additions are 24%aluminum powder and 5%activated alumina powder,and Al_(4)SiC_(4) synthesized in this sample has a hexagonal plate structure.With the synthesized Al_(4)SiC_(4) containing material added,the magnesia carbon brick has slightly increased cold modulus of rupture,basically the same modulus of elasticity and improved oxidation resistance.

关键词： aluminum powder activated alumina powder microwave synthesis Al_(4)SiC_(4) magnesia carbon bricks

UVB照射后背根神经节水平CCL2对外周痛觉调控的作用研究

在线全文

学校读者我要写书评

暂无评论

空军军医大学学报 2023年第2期44卷 123-129,134页

作者：胡杨申美伦周怡民杜尚锐韩倩郭莹邢俊玲空军军医大学军事预防医学系辐射防护医学教研室陕西西安710032 空军军医大学基础医学院学员三大队陕西西安710032 空军军医大学基础医学院学员五大队陕西西安710032

目的研究紫外线B段(UVB)照射后背根神经节(DRG)内趋化因子CC配体2(CCL2)对机械痛敏形成的影响,探讨UVB所致痛敏中CCL2对卫星胶质细胞Kir 4.1通道的调控作用。方法小鼠UVB单次足底照射造模后进行痛行为检测,之后分别在4个时间点(1、2、4... 详细信息

目的研究紫外线B段(UVB)照射后背根神经节(DRG)内趋化因子CC配体2(CCL2)对机械痛敏形成的影响,探讨UVB所致痛敏中CCL2对卫星胶质细胞Kir 4.1通道的调控作用。方法小鼠UVB单次足底照射造模后进行痛行为检测,之后分别在4个时间点(1、2、4、7 d)取DRG进行CCL2及Kir 4.1表达的测定。另取UVB造模后2 d的动物,随机分为对照组、Bindarit组、CCL2组。Bindarit组和CCL2组分别在DRG注射Bindarit和CCL2,第2日取材后,3组动物分别进行CCL2及Kir 4.1表达的测定。结果与照射前相比,UVB照射后机械痛阈值显著降低(P<0.01),第2日降至最低,第14日恢复正常;UVB造模后,CCL2和Kir 4.1均出现显著上调(P<0.01)后逐渐降低的变化过程。DRG内注射Bindarit阻断CCL2合成后,Kir 4.1显著下调(P<0.01),注射CCL2增加CCL2含量后,Kir 4.1显著上调(P<0.05)。结论UVB照射后外周痛觉敏化的产生与DRG内CCL2的上调有关,而继发于CCL2变化的Kir 4.1的上调可能起到抑制神经元兴奋性增高的作用。

关键词：紫外线背根节痛觉敏化炎症