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High strength and ductility achieved in friction stir processed Ni-Co based superalloy with fine grains and nanotwins

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Journal of Materials Science & Technology 2022年第11期106卷 162-172页

作者： Miao Wang Xingwei Huang Peng Xue shangquan wu Chuanyong Cui Qingchuan Zhang CAS Key Laboratory of Mechanical Behavior and Design of Materials University of Science and Technology of ChinaHefei 230027China Superalloy Division Institute of Metal ResearchChinese Academy of ScienceShenyang 110016China Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of ScienceShenyang 110016China School of Materials Science and Engineering University of Science and Technology of ChinaHefei 230027China

The trade-off between strength and ductility has been an enormous difficulty in the field of materials for an extended time due to their inverse correlation. In this work, friction stir processing(FSP) was for the first time performed to high-strength and high-melting-point Ni-Co based superalloy(GH4068),and enhanced strength and ductility were achieved in FSP samples. At room temperature, the FSP sample demonstrated significantly higher yield strength and ultimate tensile strength(1290 and 1670 MPa)than that of the base material(BM, 758 and 904 MPa) and advanced wrought GH4068 alloy(982 and 1291 MPa), concurrent with high tensile ductility(~24%). Compared with the BM, 70% higher yield strength of the FSP sample results from the remarkable contribution of grain-boundary and nanotwin strengthening, which has been confirmed by the multimechanistic model studied in this work. More importantly, with increasing temperature, an excellent strength-ductility synergy was obtained at 400 ℃,i.e., the yield strength of the FSP sample was increased by more than 50% compared with the BM(from789 to 1219 MPa);more interestingly, the elongation was also significantly increased from 17.9% in the BM to 28.5% in the FSP sample. Meanwhile, the Portevin-Le Chatelier effect was observed in the engineering stress-strain curve. The occurrence of this effect may be attributed to the interaction between solutes and defects like twins and mobile dislocations. Moreover, the grain refinement mechanism of FSP samples was proved to be discontinuous dynamic recrystallization.

关键词： Superalloy Friction stir processing Grain refinement Nanotwins

Nanomechanical sensor for rapid and ultrasensitive detection of tumor markers in serum using nanobody

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Nano Research 2022年第2期15卷 1003-1012页

作者： Depeng Rao Kainan Mei Tianhao Yan Yu Wang Wenjie wu Ye Chen Jianye Wang Qingchuan Zhang shangquan wu CAS Key Laboratory of Mechanical Behavior and Design of Material Department of Modern MechanicsUniversity of Science and Technology of ChinaHefei 230027China Reproductive Medicine Center Department of Obstetrics and GynecologyFirst Affiliated Hospital of Anhui Medical UniversityHefei 230022China

Early cancer diagnosis requires ultrasensitive detection of tumor markers in *** this end,we develop a novel microcantilever immunosensor using nanobodies(Nbs)as *** the smallest antibody(Ab)entity comprising an intact antigen-binding site,Nbs achieve dense receptor layers and short distances between antigen-binding regions and sensor surfaces,which significantly elevate the generation and transmission of surface *** to the inherent thiol group at the C-terminus,Nbs are covalently immobilized on microcantilever surfaces in directed orientation via one-step reaction,which further enhances the stress *** microcantilever-based nanomechanical sensor,these advantages dramatically increase the sensor ***,Nb-functionalized microcantilevers can detect picomolar concentrations of tumor markers with three orders of magnitude higher sensitivity,when compared with conventional Ab-functionalized *** proof-of-concept study demonstrates an ultrasensitive,label-free,rapid,and low-cost method for tumor marker ***,interestingly,we find Nb inactivation on sensor interfaces when using macromolecule blocking *** adsorption-induced inactivation is presumably caused by the change of interfacial properties,due to binding site occlusion upon complex coimmobilization *** findings are generalized to any coimmobilization methodology for Nbs and,thus,for the construction of high-performance immuno-surfaces.

关键词： nanobody-based biosensor stress enhancement early cancer detection tumor markers adsorption-induced inactivation

Relay-type sensing mode:A strategy to push the limit on nanomechanical sensor sensitivity based on the magneto lever

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Nano Research 2023年第2期16卷 3231-3239页

作者： Depeng Rao Tianhao Yan Zihan Qiao Yu Wang Yongpei Peng Han Tu shangquan wu Qingchuan Zhang CAS Key Laboratory of Mechanical Behavior and Design of Material Department of Modern MechanicsUniversity of Science and Technology of ChinaHefei 230027China

Ultrasensitive molecular detection and quantization are crucial for many applications including clinical diagnostics,functional proteomics,and drug discovery;however,conventional biochemical sensors cannot satisfy the stringent requirements,and this has resulted in a long-standing dilemma regarding sensitivity *** this end,we have developed an ultrasensitive relay-type nanomechanical sensor based on a magneto *** establishing the link between very weak molecular interaction and five orders of magnitude larger magnetic force,analytes at ultratrace level can produce a clearly observable mechanical ***,proof-of-concept studies showed an improved detection limit up to five orders of magnitude when employing the magneto lever,as compared with direct detection using probe *** this study,we subsequently demonstrated that the relay-type sensing mode was universal in application ranging from micromolecule to macromolecule detection,which can be easily extended to detect enzymes,DNA,proteins,cells,viruses,bacteria,chemicals,***,we found that,sensitivity was no longer subject to probe affinity when the magneto lever was sufficiently high,theoretically,even reaching single-molecule resolution.

关键词： relay-type sensing mode magneto lever nanomechanical sensor ultrasensitivity

Nanomechanical assay for ultrasensitive and rapid detection of SARS-CoV-2 based on peptide nucleic acid

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Nano Research 2023年第1期16卷 1183-1195页

作者： Yu Wang Tianhao Yan Kainan Mei Depeng Rao Wenjie wu Ye Chen Yongpei Peng Jianye Wang shangquan wu Qingchuan Zhang CAS Key Laboratory of Mechanical Behavior and Design of Material Department of Modern MechanicsUniversity of Science and Technology of ChinaHefei 230027China Reproductive Medicine Center Department of Obstetrics and GynecologyFirst Affiliated Hospital of Anhui Medical UniversityHefei 230022China

The massive global spread of the COVID-19 pandemic makes the development of more effective and easily popularized assays ***,we developed an ultrasensitive nanomechanical method based on microcantilever array and peptide nucleic acid(PNA)for the detection of severe acute respiratory syndrome-coronavirus-2(SARS-CoV-2)*** method has an extremely low detection limit of 0.1 fM(105 copies/mL)for N-gene specific sequence(20 bp).Interestingly,it was further found that the detection limit of N gene(pharyngeal swab sample)was even lower,reaching 50 copies/*** large size of the N gene dramatically enhances the sensitivity of the nanomechanical sensor by up to three orders of *** detection limit of this amplification-free assay method is an order of magnitude lower than RT-PCR(500 copies/mL)that requires *** non-specific signal in the assay is eliminated by the in-situ comparison of the array,reducing the false-positive misdiagnosis *** method is amplification-free and label-free,allowing for accurate diagnosis within 1 *** strong specificity and ultrasensitivity allow single base mutations in viruses to be distinguished even at very low ***,the method remains sensitive to fM magnitude lung cancer marker(miRNA-155).Therefore,this ultrasensitive,amplification-free and inexpensive assay is expected to be used for the early diagnosis of COVID-19 patients and to be extended as a broad detection tool.

关键词： SARS-CoV-2 nucleic acid assay nanomechanical sensoring peptide nucleic acid amplification-free

Quantifying 3D cell-matrix interactions during mitosis and the effect of anticancer drugs on the interactions

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Nano Research 2021年第11期14卷 4163-4172页

作者： Yongman Liu Jianye Wang Yong Su Xiaohai Xu Hong Liu Kainan Mei Shihai Lan Shubo Zhang Xiaoping wu Yunxia Cao Qingchuan Zhang shangquan wu CAS Key Laboratory of Mechanical Behavior and Design of Material Department of Modern MechanicsCAS Center for Excellence in Complex System MechanicsUniversity of Science and Technology of ChinaHefei230027China Reproductive Medicine Center Department of Obstetrics and GynecologyThe First Affiliated Hospital of Anhui Medical UniversityHefei230022China Anhui Province Key Laboratory of Reproductive Health and Genetics Anhui Medical UniversityHefei230022China Department of Chemical Physics University of Science and Technology of ChinaHefei230026China

The mechanical force between cells and the extracellular microenvironment is crucial to many physiological processes such as cancer metastasis and stem cell differentiation. Mitosis plays an essential role in all these processes and thus an in-depth understanding of forces during mitosis gains insight into disease diagnosis and disease treatment. Here, we develop a traction force microscope method based on monolayer fluorescent beads for measuring the weak traction force (tens of Pa) of mitotic cells in three dimensions. We quantify traction forces of human ovarian granulosa (KGN) cells exerted on the extracellular matrix throughout the entire cell cycle in three dimensions. Our measurements reveal how forces vary during the cell cycle, especially during cell division. Furthermore, we study the effect of paclitaxel (PTX) and nocodazole (NDZ) on mitotic KGN cells through the measurement of traction forces. Our results show that mitotic cells with high concentrations of PTX exert a larger force than those with high concentrations of NDZ, which proved to be caused by changes in the structure and number of microtubules. These findings reveal the key functions of microtubule in generating traction forces during cell mitosis and explain how dividing cells regulate themselves in response to anti-mitosis drugs. This work provides a powerful tool for investigating cell-matrix interactions during mitosis and may offer a potential way to new therapies for cancer.

关键词： cell division traction force paclitaxel nocodazole

Nanomechanical vibration profiling of oocytes

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Nano Research 2023年第2期16卷 2672-2681页

作者： Yongpei Peng Junhui Zhang Weiwei Xue Wenjie wu Yu Wang Kainan Mei Ye Chen Depeng Rao Tianhao Yan Jianye Wang Yunxia Cao shangquan wu Qingchuan Zhang CAS Key Laboratory of Mechanical Behavior and Design of Material Department of Modern MechanicsUniversity of Science and Technology of ChinaHefei 230027China Department of Obstetrics and Gynecology The First Affiliated Hospital of Anhui Medical UniversityHefei 230027China NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract Anhui Medical UniversityHefei 230027China Key Laboratory of Population Health Across Life Cycle Ministry of Education of the People’s Republic of ChinaAnhui Medical UniversityHefei 230027China

The beginning of a mammalian life commences with a fertilized *** study of oocytes is certainly one of the most intriguing scientific questions of our ***,we studied oocytes from a mechanical perspective and characterized the typical life activities of oocytes by nanomechanical *** the development of oocytes from the germinal vesicle(GV)stage to the zygotes,the GV stage oocytes induced a significant nanomechanical vibration,compared with the oocytes in meiosis I(MI)and meiosis II(MII)stages and *** analyzed the characteristics of mechanical vibrations of oocytes,including the amplitude as well as the *** showed that the amplitude and frequency of nanomechanical vibrations induced by oocytes were caused by the cytoskeleton(microfilaments)and the distribution of metabolic characteristics(mitochondria)within *** work provides a new perspective for clinical quality assessment and basic research of oocytes,and can open new doors for development of life science.

关键词： oocytes nanomechanical sensors nanomechaincal vibration metabolism microfilaments

Dynamic response of the cell traction force to osmotic shock

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Microsystems & Nanoengineering 2023年第5期9卷 321-331页

作者： Yongman Liu Wenjie wu Shuo Feng Ye Chen Xiaoping wu Qingchuan Zhang shangquan wu School of Biomedical Engineering Anhui Medical University230032 HefeiChina CAS Key Laboratory of Mechanical Behavior and Design of Material Department of Modern MechanicsCAS Center for Excellence in Complex System MechanicsUniversity of Science and Technology of China230026 HefeiChina

Osmotic pressure is vital to many physiological activities,such as cell proliferation,wound healing and disease ***,how cells interact with the extracellular matrix(ECM)when subjected to osmotic shock remains ***,we visualize the mechanical interactions between cells and the ECM during osmotic shock by quantifying the dynamic evolution of the cell traction *** show that both hypertonic and hypotonic shocks induce continuous and large changes in cell traction ***,the traction force varies with cell volume:the traction force increases as cells shrink and decreases as cells ***,the direction of the traction force is independent of cell volume changes and is always toward the center of the cell-substrate ***,we reveal a mechanical mechanism in which the change in cortical tension caused by osmotic shock leads to the variation in traction force,which suggests a simple method for measuring changes in cell cortical *** findings provide new insights into the mechanical force response of cells to the external environment and may provide a deeper understanding of how the ECM regulates cell structure and function.

关键词： traction healing wound

夹皮沟金矿区黑云母的化学成分及红外谱学特征

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矿物岩石 1989年第1期9卷 116-122页

作者：吴尚全吉林有色金属地质勘探公司研究所

从矿区各种不同变质岩相的代表性岩石以及海西期花岗岩中,采集了14个黑云母单矿物样品。化学分析结果证实,随岩石变质程度增高,Al_(IV)^(3+)及钾的含量降低,Mg/(Fe^(2+)+Fe^(3+))增大,(OH)降低。利用μH_2O～μ(K_2O+Na_2O)图解可以相... 详细信息

从矿区各种不同变质岩相的代表性岩石以及海西期花岗岩中,采集了14个黑云母单矿物样品。化学分析结果证实,随岩石变质程度增高,Al_(IV)^(3+)及钾的含量降低,Mg/(Fe^(2+)+Fe^(3+))增大,(OH)降低。利用μH_2O～μ(K_2O+Na_2O)图解可以相对估计生成岩石的地质环境和区分本区变质岩的原岩类型。用定量法作的红外光谱分析表明,650cm^(-1)吸收频段随含铁系数(f)增高向低频方向偏移,3400cm^(-1)频段与黑云母中(OH)含量有关,随变质程度增高,这个频段的吸光度有所降低。

关键词：黑云母变质作用红外光谱

基于DSP的电动六自由度运动模拟器位置伺服控制策略

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电气自动化 2016年第6期38卷 1-4页

作者：吴上泉工业和信息化部电子第五研究所广东广州510610

针对目前电动六自由度运动模拟器的特点,对平台进行了基于位姿反解算法的铰链空间控制,并介绍了一种新型的基于DSP的多级控制结构。在电动缸闭环控制中,引入模糊前馈PID智能算法,提高实时性与动态控制精度。基于控制算法与硬件器对平台... 详细信息

针对目前电动六自由度运动模拟器的特点,对平台进行了基于位姿反解算法的铰链空间控制,并介绍了一种新型的基于DSP的多级控制结构。在电动缸闭环控制中,引入模糊前馈PID智能算法,提高实时性与动态控制精度。基于控制算法与硬件器对平台进行试验,结果表明控制策略实时性好,动态精度高。

关键词：六自由度运动模拟器运动学反解多级控制 DSP控制器模糊前馈