检索结果-南通市图书馆

维普期刊数据库

Identification and Characterization of glnA Promoter and its corresponding Trans-regulatory Protein GlnR in the Rifamycin SV Producing Actinomycete,Amycolatopsis mediterranei U32

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2006年第12期38卷 831-843页

作者： Hao YU~(1&) Wen-Tao PENG~1 Yang LIU~1 Ting WU~2.Yu-Feng YAO~1 Ming-Xue CUI~1 Wei-Hong JIANG~1 Guo-Ping ZHAO~(1,3,4.) 1 Laboratory of Molecular Microbiology,institute of plant {2.and Ecology,{4. institutes for Biological Sciences,Chinese Academy of Sciences,{4. 2.0032.China 2.Department of Medicinal Chemistry,School of Pharmacy,Fudan University,{4. 2.0032.China 3 State Key Laboratory of Genetic Engineering & Department of Microbiology,School of Life Science,Fudan University,{4. 2.04.3,China 4.Laboratory of Disease and Health Genomics,Chinese National Human Genome Center at {4.,{4. 2.12.3,China Laboratory of Molecular Microbiology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China Department of Medicinal Chemistry School of Pharmacy Fudan University Shanghai 200032 China State Key Laboratory of Genetic Engineering & Department of Microbiology School of Life Science Fudan University Shanghai 200433 China Laboratory of Disease and Health Genomics Chinese National Human Genome Center at Shanghai Shanghai 201203 China

The genetic requirements for the transcription of glnA,encoding the major glutaminesynthetase in a rifamycin SV-producing Amycolatopsis mediterranei strain,U32.were *** experiments showed that the promoter of U32.glnA （pglnA） was likely to have two transcriptioninitiation sites:P1and P2.located 157 and 4. nucleotides （nt） upstream of the translational start codon,*** mobility shift and DNase I footprinting analyses revealed a 30 bp cis-element locatedat 4. to 75 nt downstream of P1,or 38 to 68 nt upstream of *** sequence of the cis-element displayedhigh similarity to the corresponding regions of pglnA from Streptomyces coelicolor and *** as a reporter gene,the expression levels of U32.pglnA and its deletion derivatives under differentnitrogen-source conditions were analyzed by detecting the catechol dioxygenase activities in ***54.*** J508 and *** FS10 （glnR mutant）.These in vivo studies showed that theactivation of U32.pglnA in *** required GlnR,and its binding to the U32.pglnA was furtherconfirmed by the gel mobility shift *** and heterologous expression of the U32.glnR allowed usto detect the in vitro interaction between the U32.GlnR and the corresponding pglnA *** shown by in vivo glnR inactivation and complementation indicated that GlnR is essential for theactive transcription of glnA in U32.

关键词： glnA promoter cis-element glnR Amycolatopsis mediterranei

Transiently Expressed Short Hairpin RNA Targeting 12. kDa Protein of Tobacco Mosaic Virus Interferes with Virus Infection

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2006年第1期38卷 22-28页

作者： Ming-Min ZHAO~(1,2.,De-Rong AN~(1*),Jian ZHAO~2.Guang-Hua HUANG~3,Zu-Hua HE~4.and Jiang-Ye CHEN~3 ~1 College of plant Protection,Northwest Science and Technology University of Agriculture and Forestry,Yangling 712.00,China ~2.College of Agriculture,Yangtze University,Jingzhou 4.4.2.,China ~3 State Key Laboratory of Molecular Biology,institute of Biochemistry and Cell Biology,{2.institutes for Biological Sciences, Chinese Academy of Sciences,{2.2.0031,China ~4.State Key Laboratory of plant Molecular Genetics,institute of plant {14. and Ecology,{2.institutes for Biological Sciences, Chinese Academy of Sciences,{2.{12.China College of plant Protection Northwest Science and Technology University of Agriculture and Forestry Yangling 712100 China

RNA-interference (RNAi) silences gene expression by'guiding mRNA degradation in asequence-specific *** interfering RNA (siRNA),an intermediate of the RNAi pathway,has beenshown to be very effective in inhibiting virus infection in mammalian cells and cultured plant ***,wereport that Agrobacterium tumefaciens-mediated transient expression of short hairpin RNA (shRNA) couldinhibit tobacco mosaic virus (TMV) RNA accumulation by targeting the gene encoding the replication-asso-ciated 12. kDa protein in intact plant *** results indicate that transiently expressed shRNA efficientlyinterfered with TMV *** interference observed is sequence-specific,and time-and *** expressed shRNA {2.to the TMV 12. kDa protein gene did not inhibit cucumbermosaic virus (CMV),an unrelated *** order to interfere with TMV accumulation in tobaccoleaves,it is essential for the shRNA constructs to be infiltrated into the same leaves as TMV *** support the view that RNAi opens the door for novel therapeutic procedures against virus *** propose that a combination of the RNAi technique and Agrobacterium-mediated transient expressioncould be employed as a potent antiviral treatment in plants.

关键词： tobacco mosaic virus 12. kDa protein RNA interference short hairpin RNA

Comparative analysis of temperature and CO2.fluxes for winter wheat in Tibetan Plain and North China Plain using the EMD method

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2006年第10期16卷 1066-1071页

作者： JIANG Zhaoyang, YU Qiang, LEI Shuhe, WANG Tianduo, SUN Xiaomin and ZHANG Renhua ( Chinese Academy of Meteorological Science, Beijing 100081, China institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China Mathematics Department, Ocean University of China, Qingdao 2.6071, China shanghai institute of plant physiology and Ecology, Chinese Academy of Sciences, {2.{4. China) [a]Chinese Academy of Meteorological Science Beijing China [b]Chinese Academy of Sciences Institute of Geographic sciences and Natural Resources Research Beijing China [c]Mathematics Department Ocean University of China Qingdao China [d]Chinese Academy of Sciences Shanghai Institute of Plant Physiology and Ecology Shanghai China

Comparative study of spectral properties of temperature and CO2.fluxes measured by eddy covariance method at Yucheng (36°57'N, 116°36'E, 2. m a.s.l., in the North China Plain) and at Lhasa (2.°4.'N, 91°2.'E, 3688 m a.s. 1., on the Tibetan Plateau) is described using the empirical mode decomposition (EMD) method. The main results are: (1) The intrinsic oscillation modes or intrinsic mode functions (IMFs) were extracted from data of temperature ( T) and CO2.fluxes (F) measured at Yucheng ( T1 and F1) and Lhasa ( T2.and F2. . (2. Hilbert transform was applied to these IMF components, then the Hubert-Huang spectra and the marginal spectra of these data were obtained. (3) Comparison of temperature and CO2.fluxes in North China Plain and on Tibetan Plain illustrated that the characteristic frequencies corresponding to T1, F1, T2.and F2.are 0.05 Hz, 0.03 Hz, 0.014.Hz and 0.005 Hz, respectively.

关键词： empirical mode decomposition (EMD) intrinsic mode function (IMF) flux Hilbert transform wheat Tibet.

The spike protein of severe acute respiratory syndrome (SARS) is cleaved in virus infected Vero-E6 cells

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2004年第5期14卷 400-406页

作者： Xiao Dong WU 1*, Bo SHANG1*, Rui Fu YANG2., Hao YU3*, Zhi Hai MA1*, Xu SHEN3*, Yong Yong JI1, Ying LIN , Ya Di WU1, Guo Mei LIN1, Lin TIAN1, Xiao Qing GAN1, Sheng YANG2. Wei Hong JIANG2. 1 Er Hei DAI4. Xiao Yi WANG4. Hua Liang JIANG3, You Hua XIE1, Xue Liang ZHU1, Gang PEI1, Lin LI1, Jia Rui WU1**, Bing SUN 1** 1institute of Biochemistry and Cell Biology, {4. institutes for Biological Sciences, Chinese Academy of Sciences, 32. Yueyang Road, {4. 2.0031, China. 2.0} of Microbiology and Epidemiology, institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100071, China. 3institute of plant physiology and Ecology, {4. institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, {4. 2.0031, China. 3institute of Materia Medica, {4. institutes for Biological Sciences, Chinese Academy of Sciences, 32. Taiyuan Road, {4. 2.0031, China. institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China.

Spike protein is one of the major structural proteins of severe acute respiratory syndrome-coronavirus. It is essential for the interaction of the virons with host cell receptors and subsequent fusion of the viral envelop with host cell membrane to allow infection. Some spike proteins of coronavirus, such as MHV, HCoV-OC4., AIBV and BcoV, are proteolytically cleaved into two subunits, S1 and S2. In contrast, TGV, FIPV and HCoV-2.9E are not. Many studies have shown that the cleavage of spike protein seriously affects its function. In order to investigate the maturation and proteolytic processing of the S protein of SARS CoV, we generated S1 and S2.subunit specific antibodies (Abs) as well as N, E and 3CL protein-specific Abs. Our results showed that the antibodies could efficiently and specifically bind to their corresponding proteins from *** expressed or lysate of SARS-CoV infected Vero-E6 cells by Western blot analysis. Furthermore, the anti-S1 and S2.Abs were proved to be capable of binding to SARS CoV under electron microscope observation. When S2.Ab was used to perform immune precipitation with lysate of SARS-CoV infected cells, a cleaved S2.fragment was detected with S2.specific mAb by Western blot analysis. The data demonstrated that the cleavage of S protein was observed in the lysate, indicating that proteolytic processing of S protein is present in host cells.

关键词： SARS-CoV spike protein nucleocapsid protein envelope protein 3CL protease polyclonal antibody.

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

乙烯利对植物排泌乳液的刺激作用

Journal of Integrative plant Biology 1975年第1期 49-55页

作者： LABORATORY OF PHYTOHORMONE, shanghai institute OF {2.physiology 上海植物生理研究所激素研究室

用国产乙烯利处理分属四科的五种植物:巴西三叶橡胶树(Hevea brasiliensis,大戟科),印度紫檀(Pterocarpus indicus,豆科),吐鲁香(Myroxylon balsamum,豆科),漆树(Rhussp,漆树科),马尾松(Pinus massoniana,松科),从树干采割乳液(胶乳,树脂等)。处理植株乳液排泌量明显地大于对照。看来乙烯利(乙烯)促进植物排泌乳液的现象具有一定的普遍性。本文还讨论了乙烯利在刺激植物排泌乳液中可能起的作用。

关键词：乙烯利乳液 2.氯乙基膦酸植物生长调节物质三叶橡胶树泌乳植物刺激作用

Expression of small interfering RNAs targeting to development-related genes of Ditylenchus destructor in sweetpotato improves host resistance during infection

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Expression of small interfering RNAs targeting to developmen...

A PPR Protein Is Invovled in dark-induced leaf senescence in Arabidopsis

2.11全国植物生物学研讨会

作者： Zhaorong Wei~1,Peng Zhang~(1,2.) 1 institute of plant {2.& Ecology,{4. institutes for Biological Sciences,Chinese Academy of Sciences,300 Fenglin Road,{4. 2.0032.China 2.Chenshan plant Science Research Center,Chinese Academy of Sciences,Chenshan Botanical Garden,3888 Chenhua Road,{4. 2.1602.China

Ditylenchus destructor,a plant parasitic nematode frequently called stem nematode,is one of the major pathogens of sweetpotato[Ipomoea batatas(L.) Lam]. In order to improve stem nematode resistance,sweetpotato expressing small interfering RNAs(siRNAs)to supress the expression of two key genes related to stem nematode development was *** first one is a paramyosin ortholog gene (dd-unc-15) implicated in physically interacting with myosin heavy chain A(MHC A) in striated muscle,and the other is a citrate synthase gene(dd-cts) predicted to be mitochondrial and required for embryonic *** expression levels of siRNAs derived from corresponding double stranded RNA interference constructs in transgenic sweet potato plants were verified by Northern *** was found that, at 10,2. and 30 days after inoculation,the amount of nematodes in the storage roots

关键词： sweet potato anthocyanin biosynthesis expression regulation flavonoids

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

A PPR Protein Is Invovled in dark-induced leaf senescence in...

Burkholderia pickettii中D-乙内酰脲酶基因(dha)的克隆、测序及表达

2.11全国植物生物学研讨会

作者： Fang Liu,Yu-Ting Wang,Si-Ting Chen,Fang-Qing Guo~* institute of plant {2.& Ecology,shanghai institutes for Biological Sciences, Chinese Academy of Sciences,shanghai,2.0032

To gain insight into mechanisms in which leaf senescence is regulated,we performed a web-based microarray analysis of the detached leaves of wild type plants after dark *** screening T-DNA insertion mutants corresponding to the candidate genes,we isolated a knock-out mutant of the gene encoding a pentatricopeptide repeat(PPR) protein,named as AtDIL1(Dark-Induced Leaf Senescence 1).AtDIL1 protein contains tandemly-repeated PPR motifs that bind specific *** proteins are thought to be central regulators of chloroplast and

关键词： AtDIL1 leaf senescence dark induction

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

生物工程学报 2002年第2期18卷 149-154页

作者：许祯姜卫红焦瑞身杨蕴刘中国科学院上海植物生理生态研究所上海200032

对一株能转化D ,L 对羟基苯乙内酰脲为D 对羟基苯甘氨酸的菌株MMR0 0 3进行了细菌分类学鉴定 ,该菌为皮氏伯克霍尔德氏菌 (Burkholderiapickettii)。实验通过Southern杂交 ,部分文库构建和筛选 ,并经一系列亚克隆测序分析 ,获得一长度为... 详细信息

对一株能转化D ,L 对羟基苯乙内酰脲为D 对羟基苯甘氨酸的菌株MMR0 0 3进行了细菌分类学鉴定 ,该菌为皮氏伯克霍尔德氏菌 (Burkholderiapickettii)。实验通过Southern杂交 ,部分文库构建和筛选 ,并经一系列亚克隆测序分析 ,获得一长度为 1374.p的完整开放阅读框 ,编码 4. 8个氨基酸的D 乙内酰脲酶基因。用该基因序列构建的高表达质粒pXZPH2.化E .coliBL2.1(DE3) ,经IPTG诱导后 ,检测到D 乙内酰脲酶活性。该基因编码的氨基酸序列经Blast同源比较分析与放射形土壤杆菌NRRLB112.91所产相应酶有 85 %的同源性。以D ,L 对羟基苯乙内酰脲为底物测得的表达酶的活力为 0 6 6u mL ,比相同条件下所测出发菌株MMR0 0 3的酶活提高了

关键词：皮氏伯克霍尔德氏菌 D-乙内酰脲酶基因克隆表达序列分析

维普期刊数据库

Study of Complex Redundancy-Pleiotropy Network of Arabidopsis Ethylene Receptors

在线全文

学校读者我要写书评

暂无评论

Study of Complex Redundancy-Pleiotropy Network of Arabidopsi...