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Interaction between Mnk2 and CBC^(VHL) ubiquitin ligase E3 complex

Science china(Life Sciences) 2006年第3期49卷 265-273页

作者： WANG Pingzhang, WANG Xin, WANG Feng, CAI Tianjing & LUO Ying Chinese National Human Genome Center, Beijing 100176, china Institute of Basic Medical Sciences, Chinese Acadamy of Medical Sciences, Beijing 100005, china shanghai genomics inc., shanghai 201203, china Chinese National Human Genome Center Beijing 100176 China Institute of Basic Medical Sciences Chinese Acadamy of Medical Sciences Beijing 100005 China Chinese National Human Genome Center Beijing 100176 China shanghai genomics inc. Shanghai 201203 China

MAP kinase-interacting kinase-2 (Mnk2) is one of the downstream kinases activated by MAP kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E), although the role of eIF4E phosphorylation and the role of Mnk2 in the process of protein translation are not well understood. Except for eIF4E, other physiological substrates of Mnk2 are still unidentified. To look for these unidentified substrates and to reveal the physiological function of Mnk2, we performed a yeast two-hybrid screening with Mnk2 as the bait. The results demonstrated Mnk2 could interact with VHL (von Hippel-Lindau tumor suppressor), Rbx1 (ring-box 1) and Cul2 (Cullin2) proteins in yeast cells. Furthermore, we validated the interaction between Mnk2 and VHL proteins in mammalian cells by co-immunoprecipitation analysis. Because the three proteins VHL, Rbx1 and Cul2 are all components of the CBCVHL ubiquitin ligase E3 complex, it has been shown that Mnk2 can interact with CBCVHL complex, and is probably one of the new substrates of the CBCVHL complex. Furthermore, during the interaction of Mnk2 with von Hippel-Lindau (VHL) tumor suppressor- binding protein 1 (VBP1), it appears that Mnk2 also joins to modulate cell shape as VBP1 plays an important role in the process of the maturation of the cytoskeleton and in the process of morphogenesis.

关键词： Mnk2, yeast two-hybrid, CBCVHL ubiquitin ligase E3 complex, VBP1.

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Recent progress in 8igenomic research of liver cancer

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Science china(Life Sciences) 2009年第1期52卷 24-30页

作者： HAN ZeGuang shanghai-MOST Key Laboratory for Disease and Health genomics,Chinese National Human Genome Center at shanghai,shanghai 201203,china shanghai-MOST Key Laboratory for Disease and Health genomics Chinese National Human Genome Center at Shanghai Shanghai China

Along the course of occurrence and development of liver cancer,the corresponding somatic cells accumulate some important genetic *** variations may be divided into two *** the genetic changes closely related to etiology of liver cancer,the well-known cases inc.ude insertion and integration of the hepatitis B virus(HBV) DNA after infection,and mutations at site 249 of the tumor suppressor gene p53 induced by exposure to aflatoxin *** secondary genetic changes inc.ude amplification and deletion of certain chromosome regions,mutations in p53 at the sites other than 249,as well as the mutational activation of the Wnt/β-catenin signal *** tumor cells with these genetic variations may gradually become the dominant clones under evolutionary ***,identification of genetic susceptible against risk of liver malignancy is also an important aspect of research in this field.

关键词： hepatocellular carcinoma chromosomal instability gene mutation oncogene tumor suppressor gene

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Development and application of genotyping technologies

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Science china(Life Sciences) 2009年第1期52卷 17-23页

作者： SHI JinXiu,WANG Ying & HUANG Wei shanghai-MOST Key Laboratory of Health and Disease genomics,Chinese National Human Genome Center at shanghai,shanghai 201203,china shanghai-MOST Key Laboratory of Health and Disease genomics Chinese National Human Genome Center at Shanghai Shanghai China

With the completion of Human Genome Project,International HapMap Project and the publication of copy number variation in human genome,a great number of accurate,rapid,and cost-effective technologies for SNP analysis have been developed,promoting the research of the complex *** article presents a review of widely used genotyping techniques,and the progress and prospect in the study of complex diseases in terms of the projects and achievements of Chinese National Human Genome Center at shanghai(CHGCs).

关键词： single nucleotide polymorphism copy number variation complex disease

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Perspectives of DNA microarray and next-generation DNA sequencing technologies

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Science china(Life Sciences) 2009年第1期52卷 7-16页

作者： TENG XiaoKun1 & XIAO HuaSheng1,2 1 National Engineering Center for Biochip at shanghai,shanghai 201203,china 2 shanghai-MOST Key Laboratory of Health and Disease genomics,Chinese National Human Genome Center,shanghai 201203,china National Engineering Center for Biochip at shanghai Shanghai China shanghai-MOST Key Laboratory of Health and Disease genomics Chinese National Human Genome Center Shanghai China

DNA microarray and next-generation DNA sequencing technologies are important tools for high-throughput genome research,in revealing both the structural and functional characteristics of *** the past decade the DNA microarray technologies have been widely applied in the studies of functional genomics,systems biology and *** next-generation DNA sequencing method was first introduced by the 454 Company in 2003,immediately followed by the establishment of the Solexa and Solid techniques by other biotech *** it has not been long sinc. the first emergence of this technology,with the fast and impressive improvement,the application of this technology has extended to almost all fields of genomics research,as a rival challenging the existing DNA microarray *** paper briefly reviews the working princ.ples of these two technologies as well as their application and perspectives in genome research.

关键词： DNA microarray next generation DNA sequencing genome

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Progress in the detection of human genome structural variations

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Science china(Life Sciences) 2009年第6期52卷 560-567页

作者： WU XueMei1,3 & XIAO HuaSheng1,2 1 Center of Functional genomics,Key Laboratory of System Biology,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 200031,china 2 National Engineering Center for Biochip at shanghai,shanghai 201203,china 3 Graduate School of the Chinese Academy of Sciences,shanghai 200031,china Center of Functional genomics Key Laboratory of System Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Graduate School of the Chinese Academy of Sciences Shanghai China National Engineering Center for Biochip at shanghai Shanghai China

The emerging of high-throughput and high-resolution genomic technologies led to the detection of submicroscopic variants ranging from 1 kb to 3 Mb in the human *** variants inc.ude copy number variations(CNVs),inversions,insertions,deletions and other complex rearrangements of DNA *** paper briefly reviews the commonly used technologies to discover both genomic structural variants and their potential ***,we highlight the array-based,PCR-based and sequencing-based assays,inc.uding array-based comparative genomic hybridization(aCGH),representational oligonucleotide microarray analysis(ROMA),multiplex amplifiable probe hybridization(MAPH),multiplex ligation-dependent probe amplification(MLPA),paired-end mapping(PEM),and next-generation DNA sequencing ***,we discuss the limitations and challenges of current assays and give advices on how to make the database of genomic variations more reliable.

关键词： structural variation cytogenetic microarray PCR paired end mapping(PEM) next-generation sequencing

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miRNAs modulate the drug response of tumor cells

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Science china(Life Sciences) 2009年第9期52卷 797-801页

作者： WU XueMei1,3 & XIAO HuaSheng1,2 1 Center of Functional genomics,Key Laboratory of System Biology,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 200031,china 2 National Engineering Center for Biochip at shanghai,shanghai 201203,china 3 Graduate University of Chinese Academy of Sciences,shanghai 200031,china Center of Functional genomics Key Laboratory of System Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Graduate University of Chinese Academy of Sciences Shanghai China National Engineering Center for Biochip at shanghai Shanghai China

Chemotherapy is one of the major treatments of malignant carcinomas. However,its efficiency is affected by both intrinsic and acquired resistance to anticancer drugs. The cellular mechanisms of drug resistance inc.ude the overexpression of energy-dependent transporters that eject anticancer drugs from cells such as p-glycoprotein and multidrug resistance related protein (MRP),the mutation of drug targets,the activation of DNA repair pathways,the defects in cellular death pathways and so on. The genetic and epigenetic changes of these genes can lead to cancer drug resistance. Among these mechanisms,microRNAs (miRNAs) which are critical and essential for many important processes such as development,differentiation,and even carcinogenesis have been reported to regulate the chemosen-sitivity of tumor cells. In this paper we briefly review the relationship between miRNA and cancer drug resistance.

关键词： cancer drug resistance miRNAs

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Learn to fight from the war

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Chinese Science Bulletin 2009年第13期54卷 2157-2158页

作者： ZHAO GuoPing shanghai-MOST Key Laboratory for Disease and Health genomics,Chinese National Human Genome Center at shanghai,shanghai 201203,china Lab of Synthetic Biology,shanghai Institutes of Biological Sciences,Chinese Academy of Sciences,shanghai 200031,china National Engineering Research Center for Biochip Technology,shanghai Biochip Co.Ltd.,shanghai 201203,china School of Life Sciences,Fudan University,shanghai 200433,china ZHONG Yang School of Life Sciences,Fudan University,shanghai 200433,china shanghai Center for Bioinformation Technology,shanghai 200235,china 1shanghai-MOST Key Laboratory for Disease and Health genomics Chinese National Human Genome Center at Shanghai Shanghai 201203 China. 2Lab of Synthetic Biology Shanghai Institutes of Biological Sciences Chinese Academy of Sciences Shanghai 200031 China. National Engineering Research Center for Biochip Technology Shanghai Biochip Co. Ltd. Shanghai 201203 China. 4School of Life Sciences Fudan University Shanghai 200433 China. 5shanghai Center for Bioinformation Technology Shanghai 200235 China.

In March 2009,a novel type A (H1N1) influenza broke out in North America and rapidly spread all over the world.

关键词：世界卫生组织战争军备竞赛 WHO 流感

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china's Software Industry Aiming at a Higher Standard

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Wuhan University Journal of Natural Sciences 2001年第Z1期6卷 355-358页

作者： Ju De hua Application Solutions & Technologies,inc.shanghai, shanghai 200233, china Application Solutions & Technologies Inc Shanghai Shanghai China

The current status of the china's software industry is introduced, inc.uding the great potential opportunities and many problems across the path to success. The main measures are discussed to keep abreast of the tim... 详细信息

关键词： china's software industry offshore service software process improvement

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Identification and Characterization of glnA Promoter and its Corresponding Trans-regulatory Protein GlnR in the Rifamycin SV Producing Actinomycete,Amycolatopsis mediterranei U32

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Acta Biochimica et Biophysica Sinica 2006年第12期38卷 831-843页

作者： Hao YU~(1&) Wen-Tao PENG~1 Yang LIU~1 Ting WU~2 Yu-Feng YAO~1 Ming-Xue CUI~1 Wei-Hong JIANG~1 Guo-Ping ZHAO~(1,3,4*) 1 Laboratory of Molecular Microbiology,Institute of Plant Physiology and Ecology,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 200032,china 2 Department of Medicinal Chemistry,School of Pharmacy,Fudan University,shanghai 200032,china 3 State Key Laboratory of Genetic Engineering & Department of Microbiology,School of Life Science,Fudan University,shanghai 200433,china 4 Laboratory of Disease and Health genomics,Chinese National Human Genome Center at shanghai,shanghai 201203,china Laboratory of Molecular Microbiology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China Department of Medicinal Chemistry School of Pharmacy Fudan University Shanghai 200032 China State Key Laboratory of Genetic Engineering & Department of Microbiology School of Life Science Fudan University Shanghai 200433 China Laboratory of Disease and Health genomics Chinese National Human Genome Center at Shanghai Shanghai 201203 China

The genetic requirements for the transcription of glnA,encoding the major glutaminesynthetase in a rifamycin SV-producing Amycolatopsis mediterranei strain,U32,were *** experiments showed that the promoter of U32 glnA （pglnA） was likely to have two transcriptioninitiation sites:P1and P2,located 157 and 45 nucleotides （nt） upstream of the translational start codon,*** mobility shift and DNase I footprinting analyses revealed a 30 bp cis-element locatedat 45 to 75 nt downstream of P1,or 38 to 68 nt upstream of *** sequence of the cis-element displayedhigh similarity to the corresponding regions of pglnA from Streptomyces coelicolor and *** as a reporter gene,the expression levels of U32 pglnA and its deletion derivatives under differentnitrogen-source conditions were analyzed by detecting the catechol dioxygenase activities in ***54,*** J508 and *** FS10 （glnR mutant）.These in vivo studies showed that theactivation of U32 pglnA in *** required GlnR,and its binding to the U32 pglnA was furtherconfirmed by the gel mobility shift *** and heterologous expression of the U32 glnR allowed usto detect the in vitro interaction between the U32 GlnR and the corresponding pglnA *** shown by in vivo glnR inactivation and complementation indicated that GlnR is essential for theactive transcription of glnA in U32.

关键词： glnA promoter cis-element glnR Amycolatopsis mediterranei

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Current research status of immunology in the genomic era

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Science china(Life Sciences) 2009年第1期52卷 43-49页

作者： LI HaoWen1,LI JinZhi1,ZHAO GuoPing2 & WANG Ying1,2 1 shanghai Institute of Immunology,Institute of Medical Science,shanghai Jiaotong University School of Medicine,shanghai 200025,china 2 shanghai-MOST Key Laboratory of Health and Disease genomics,Chinese National Human Genome Center,shanghai 201203,china shanghai Institute of Immunology Institute of Medical Science Shanghai Jiaotong University School of Medicine Shanghai 200025 China.

This review updates the current status of immunology research under the influence of genomics,both conceptually and *** particularly highlights the advantages of employing the high-throughput and large-scale technology,the large genomic database,and bioinformatic power in the immunology *** fast development in the fields of basic immunology,clinical immunology(tumor and infectious immunology) and vaccine designing is illustrated with respect to the successful usage of genomic *** also speculate the future research directions of immunology in the era of genomics and post-genomics.

关键词： genomics immunology clinical immunology vaccine design

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