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Isolation and analysis of a TIR-specific promoter from poplar

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Forestry Studies in China 2007年第2期9卷 95-106页

作者： zheng Hui-quan lin shan-zhi zhang qian zhang zhen-zhen zhang zhi-yi Lei Yang Hou Lu Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants Ministry of Education Beijing Forestry UniversityBeijing 100083 P. R. China

A 5＇ flanking region of the well-conserved Toll/interleukin-1 receptor domain （TIR）-encoding sequence was isolated from the genomic DNA ofMelampsora magnusiana Wagner resistant clones of hybrid triploid poplars [（Populus tomentosa × P. bolleana） × P. tomentosa]. Sequencing results and alignment analysis show that the obtained TIR-specific promoter （named as PtTIRp01） was 1,732 bp in length; moreover 3＇ region of the PtTIRp01 contains a responds to the 5＇ composition of TIR-NBS type gene PtDRG02, of 747 bp long 5＇ region of TIR-NBS type gene PtDRG02 and its 398 bp complete TIR-encoding sequence, which significantly corindicating that the obtained TIR-specific promoter region consists upstream region of promoter （985 bp）. It was found that the 5＇ region of TIR-NBS type gene PtDRG02 was characterized in the downstream region of the transcriptional start, named as 5＇-untranslated region （5＇ UTR）, consisting of one 93 bp 5＇-untranslation exon, one 213 bp intron and one 441 bp TIR-encoding open reading frame （ORF）. In addition, several putative cis-acting motifs were present in the obtained TIR-specific promoter of PtDRG02, including one TATA box, one GC-rich, one AT-rich, one P-box, one 3-AF1 binding site, two CAAT boxes, two GT-1 motifs, three typical W-boxes, four I-boxes, and one multi-cis-acting fragment （MCF）. The latter contains five types of regulatory elements （E4, G-box, ABRE motif, box 1 and HVA 1 s）, most of which were homologous to the cis-acting regulatory elements involved in the activation of defense genes in plants. Thus, it can be suggested that TIR-specific promoter might be a pathogen-inducible promoter and be necessary for the inducible expression of defense-related genes. Key words Toll/interleukin- 1 receptor domain, promoter, Cis-acting element, poplar

关键词： Toll/interleukin- 1 receptor domain promoter Cis-acting element poplar

Assessment of Rhizospheric Microorganisms of Transgenic Populus tomentosa with Cowpea Trypsin Inhibitor (CpTI) Gene

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Forestry Studies in China 2005年第3期7卷 28-34页

作者： zhang qian zhang zhi-yi lin shan-zhi lin yuan-zhen Yang Le Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants Ministry of Education Beijing Forestry University Beijing 100083 P. R. China

To have a preliminary insight into biosafety of genetically transformed hybrid triploid poplars （Populus tomentosa × P bolleana）× P. tomentosa with the cowpea trypsin inhibitor （CpTD gene, two layers of rhizospheric soil （from 0 to 20cm deep and from 20 to 40cm deep, respectively） were collected for microorganism culture, counting assay and PCR analysis to assess the potential impact of transgenic poplars on non-target microorganism population and transgene dispersal. When the same soil layer of suspension stock solution was diluted at both 1：1000 and 1：10000 rates, there were no significant differences in bacterium colony numbers between the inoculation plates of both transgenic and non-transgenic poplars. The uniform results were revealed for both soil layer suspension solutions of identical poplars at both dilution rates except for non-transgenic poplars at 1：10000 dilution rates from the same type of soil. No significant variation in morphology of both Gram-positive and Gram-negative bacteria was observed under the microscope. The potential transgene dispersal from root exudates or fallen leaves to non-target microbes was repudiated by PCR analysis, in which no CpTI gene specific DNA band was amplified for 15 sites of transgenic rhizospheric soil samples. It can be concluded that transgenic poplar with the CpTI gene has no severe impact on rhizospheric microorganisms and is tentatively safe to surrounding soil micro-ecosystem.

关键词： transgenic poplar CpTI gene rhizospheric microorganisms ecological risk soil profile

High Level Expression of Glucose-6-phosphate Dehydrogenase Gene PsG6PDH from Populus suaveolens in E. coli

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Forestry Studies in China 2005年第3期7卷 35-38页

作者： lin yuan-zhen zhang zhi-yi lin shan-zhi zhang qian Wang Xin Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants Ministry of Education Beijing Forestry University Beijing 100083 P. R. China

In order to investigate the functions of the gene PsG6PDH and the mechanisms underlying freezing tolerance of Populus suaveolens, the recombinant expression vector pET-G （pET30a-G6PDH）, which contained full encoding region of PsG6PDH gene, was established. The recombinant was identified by lawn-PCR and double enzyme digestion and then transformed into expression host XA90 and induced by isopropyl-a-D-thiogalactoside （IPTG） to express 100 kD polypeptide of G6PDH fusion protein. The results showed that the expressed amount of the fusion protein culminated after 1 mmol·L^-1 IPTG treatment for 4h and that pET-G product was predominately soluble and not extra-cellular secreting.

关键词： Populus suaveolens glucose 6-phosphate dehydrogenase PsG6PDH prokaryotic expression

Construction and Characterization of cDNA Library from Water-Stressed Plantlets Regenerated in vitro of Populus hopeiensis

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Forestry Studies in China 2005年第3期7卷 39-42页

作者： Wang Ze-liang zhang zhi-yi lin shan-zhi lin yuan-zhen zhang qian Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants Ministry of Education Beijing Forestry University Beijing 100083 P. R. China

In order to isolate and clone water-stress-responsive genes, total RNA was extracted from water-stressed plantlets regenerated in vitro of Populus hopeiensis using a QIAGEN RNeasy Plant Mini Kit. CDNA, synthesized by LD-PCR with the SMART cDNA Library Construction Kit, was in vitro packaged into a phage λTriplEx2 vector. The resulting primary library and amplified library have a titer of 1.68×10^6 and 1.69×10^9 pfu·mL^-1 respectively. The combination ratio reached 98.8% and the average size of inserts was about 800 bp. In addition, the percentage of inserted fragments （〉400bp） was approximately 90%. The results indicate that a cDNA library has been successfully constructed.

关键词： cDNA library Populus hopeiensis water-stressed plantlet characterization

Macrophage-NLRP3 Inflammasome Activation Exacerbates Cardiac Dysfunction after Ischemic Stroke in a Mouse Model of Diabetes

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Neuroscience Bulletin 2020年第9期36卷 1035-1045页

作者： Hong-Bin lin Guan-shan Wei Feng-Xian Li Wen-Jing Guo Pu Hong Ya-qian Weng qian-qian zhang Shi-yuan Xu Wen-Bin Liang zhi-Jian You Hong-Fei zhang Department of Anesthesiology Zhujiang Hospital of Southern Medical UniversityGuangzhou 510220China Department of Anesthesiology Shenzhen SAMII Medical CenterShenzhen 518118China Department of Anesthesiology The Second Affiliated HospitalShantou University Medical CollegeShantou 515041China University of Ottawa Heart Institute and Department of Cellular and Molecular Medicine University of OttawaOntarioK1NCanada

Ischemic stroke is one of the leading causes of death *** the post-stroke stage,cardiac dysfunction is common and is known as the brain-heart *** mellitus worsens the post-stroke ***-induced systemic inflammation is the major causative factor for the sequential complications,but the mechanism underlying the brain-heart interaction in diabetes has not been *** NLRP3(NLR pyrin domain-containing 3) inflammasome,an important component of the inflammation after stroke,is mainly activated in M1-polarized *** this study,we found that the cardiac dysfunction induced by ischemic stroke is more severe in a mouse model of type 2 ***,M1-polarized macrophage infiltration and NLRP3 inflammasome activation increased in the cardiac ventricle after diabetic ***,the NLRP3 inflammasome inhibitor CY-09 restored cardiac function,indicating that the Ml-polarized macrophage-NLRP3 inflammasome activation is a pathway underlying the brain-heart interaction after diabetic stroke.

关键词： Ischemic stroke Diabetes mellitus Cardiac dysfunction NLRP3 inflammasome Macrophage

Production of 87Rb Bose–Einstein Condensate with a Simple Evaporative Cooling Method

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Chinese Physics Letters 2020年第3期37卷 45-49页

作者： Rehman Fazal Jia-zhen Li zhi-Wen Chen yuan Qin Ya-yi lin Zuan-Xian zhang shan-Chao zhang Wei Huang Hui Yan Shi-Liang Zhu Guangdong Provincial Key Laboratory of Quantum Engineering and Quantum Materials GPETR Center for Quantum Precision Measurement and SPTESouth China Normal UniversityGuangzhou 510006 National Laboratory of Solid State Microstructures School of PhysicsNanjing UniversityNanjing 210093

A Bose–Einstein condensate with a large atom number is an important experimental platform for quantum simulation and quantum information *** optical dipole trap is the a conventional way to hold the ultracold atoms,where an atomic cloud is evaporatively cooled down before reaching the Bose–Einstein condensate.A carefully designed trap depth controlling curve is typically required to realize the optimal evaporation *** present and demonstrate a simple way to optimize the evaporation cooling in a crossed optical dipole trap.A polyline shape optical power control profile is easily obtained with our method,by which a pure Bose–Einstein condensate with atom number 1.73×10^5 is ***,we numerically simulate the optimal evaporation cooling using the parameters of our apparatus based on a kinetic *** to the simulation results,our evaporation cooling shows a good *** believe that our simple method can be used to quickly realize evaporation cooling in optical dipole traps.

关键词： theory. Einstein dipole

Metformin accelerates bone fracture healing by promoting type H vessel formation through inhibition of YAP1/TAZ expression

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Bone Research 2023年第3期11卷 625-637页

作者： Zhe Ruan Hao yin Teng-Fei Wan zhi-Rou lin Shu-shan Zhao Hai-Tao Long Cheng Long Zhao-Hui Li Yu-Qi Liu Hao Luo Liang Cheng Can Chen Min Zeng zhang-yuan lin Rui-Bo Zhao Chun-yuan Chen zhen-Xing Wang zheng-Zhao Liu Jia Cao yi-yi Wang ling Jin yi-Wei Liu Guo-qiang Zhu Jing-Tao Zou Jiang-shan Gong yi Luo yin Hu Yong Zhu Hui Xie Department of Orthopedics Xiangya HospitalCentral South UniversityChangshaHunan 410008China Movement System Injury and Repair Research Center Xiangya HospitalCentral South UniversityChangshaHunan 410008China Hunan Key Laboratory of Angmedicine ChangshaHunan 410008China Angmedicine Research Center of Central South University ChangshaHunan 410008China The First Affiliated Hospital Department of Metabolism and EndocrinologyHengyang Medical SchoolUniversity of South ChinaHengyangHunan 421001China National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan 410008China

Due to increasing morbidity worldwide,fractures are becoming an emerging public health *** study aimed to investigate the effect of metformin on the healing of osteoporotic as well as normal *** H vessels have recently been identified as a bone-specific vascular subtype that supports ***,we show that metformin accelerated fracture healing in both osteoporotic and normal ***,metformin promoted angiogenesis in vitro under hypoxia as well as type H vessel formation throughout fracture ***,metformin increased the expression of HIF-1α,an important positive regulator of type H vessel formation,by inhibiting the expression of YAP1/TAZ in calluses and hypoxia-cultured human microvascular endothelial cells(HMECs).The results of HIF-1αor YAP1/TAZ interference in hypoxia-cultured HMECs using si RNA further suggested that the enhancement of HIF-1αand its target genes by metformin is primarily through YAP1/TAZ ***,overexpression of YAP1/TAZ partially counteracted the effect of metformin in promoting type H vessel-induced angiogenesis-osteogenesis coupling during fracture *** summary,our findings suggest that metformin has the potential to be a therapeutic agent for fractures by promoting type H vessel formation through YAP1/TAZ inhibition.

关键词： YAP1 healing fracture

甜杨PsG6PDH基因超表达对提高烟草抗寒冻性的影响

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西北农林科技大学学报（自然科学版） 2010年第3期38卷 125-131页

作者：林元震张志毅郭海林善枝张谦华南农业大学林学院广东广州510642 北京林业大学林木花卉遗传教育部重点实验室北京100083 水利部水土保持植物开发管理中心北京100038

【目的】研究甜杨葡萄糖-6-磷酸脱氢酶(PsG6PDH)基因超表达对烟草抗寒冻性的影响,为烟草的抗寒冻育种提供理论依据。【方法】构建甜杨PsG6PDH基因的植物表达载体pBGⅠ,采用叶盘转化法转化烟草,对转化烟草植株低温驯化后的形态、生理生... 详细信息

【目的】研究甜杨葡萄糖-6-磷酸脱氢酶(PsG6PDH)基因超表达对烟草抗寒冻性的影响,为烟草的抗寒冻育种提供理论依据。【方法】构建甜杨PsG6PDH基因的植物表达载体pBGⅠ,采用叶盘转化法转化烟草,对转化烟草植株低温驯化后的形态、生理生化指标进行测定与分析。【结果】PCR检测及Southern杂交结果显示,甜杨PsG6PDH基因已整合到转基因烟草的基因组中。低温试验表明,未经冷驯化的对照烟草较转基因烟草早出现萎蔫,恢复正常生长也较晚;经过冷驯化在接近0℃处理24h的对照烟草叶片基本萎蔫,而转基因烟草仍然正常。在0℃及更低温度胁迫下,转基因烟草的相对电导率一直保持在35%左右,而对照烟草的相对电导率则从35%上升到90%;随着胁迫时间的延长,转基因烟草的SOD、POD活性升高,而MDA含量下降。【结论】甜杨PsG6PDH基因能够提高植物的抗寒冻性,可以作为改良植物抗寒冻性的新的外源基因。

关键词：甜杨葡萄糖-6-磷酸脱氢酶烟草抗寒冻性超表达

SAPT:a synchrotron-based proton therapy facility in shanghai

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Nuclear Science and Techniques 2023年第10期34卷 36-46页

作者： Man-Zhou zhang De-Ming Li Li-Ren Shen Hai-Rong zhang zhi-ling Chen Han-Wen Du Ming Gu Rui Li De-Kang Liu Yue-Hu Pu Jun-Feng Yu Jian-Feng Chen Chu Chen Chun-Long Guo Hao Guo Ge-Yang Jiang zhi-qiang Jiang lin Jin Wen-Jing Li Xiu-Fang Li Ye lin Ming Liu Yong-Hao Liu Ya-Juan Liu Ming Lv Qing-Ru Mi Lian-Hua Ouyang Wei-Guo Shi Hang Shu Qi-Sheng Tang Kun Wang zhi-shan Wang Jun Wu Xiao-Bing Wu Jia-qiang Xu Wen-zhen Xu Chong-Xian yin Cheng Yu Ren-Xian yuan Qi-Bing yuan Hai-Qun zhang Miao zhang Wen-zhi zhang Li-ying Zhao Wei-Min Zhou Shou-Xian Fang Xi-Dong Sun zhen-Tang Zhao shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China shanghai Advanced Research Institute Chinese Academy of SciencesShanghai 201204China APACTRON Particle Equipment Co. Ltd.Shanghai 201815China The Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China

Because of its excellent dose distribution,proton therapy is becoming increasingly popular in the medical application of cancer treatment.A synchrotron-based proton therapy facility was designed and constructed in *** synchrotron,beam delivery system,and other technical systems were commissioned and reached their expected *** a clinical trial of 47 patients was finished,the proton therapy facility obtained a registration certificate from the National Medical Products *** characteristics of the accelerator and treatment systems are described in this article.

关键词： Proton therapy Synchrotron Slow extraction Gantry Beam delivery system