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Integrative analysis of aberrant Wnt signaling in hepatitis B virus-related hepatocellular carcinoma

World Journal of Gastroenterology 2015年第20期21卷 6317-6328页

作者： shan-long ding Zi-Wei Yang Jie Wang Xiao-Lei Zhang Xiang-Mei Chen Feng-Min Lu Department of Microbiology and Infectious Disease Center School of Basic Medical SciencesPeking University Health Science Center

AIM: To comprehensively understand the underlying molecular events accounting for aberrant Wnt signaling activation in hepatocellular carcinoma(HCC).METHODS: This study was retrospective. The HCC tissue specimens used in this research were obtained from patients who underwent liver surgery. The Catalogue of Somatic Mutations in Cancer(COSMIC) database was searched for the mutation statuses of CTNNB1, TP53, and protein degradation regulator genes of CTNNB1. Dual-luciferase reporter assay was performed with TOP/FOP reporters to detect whether TP53 gain-of-function(GOF) mutations could enhance the transcriptional activity of Wnt signaling. Methylation sensitive restriction enzyme-quantitative PCR was used to explore the methylation status of Cp G islands located in the promoters of APC, SFRP1, and SFRP5 in HCCs with different risk factors. Finally, nestedreverse transcription PCR was performed to examine the integration of HBx in front of LINE1 element and the existence of HBx-LINE1 chimeric transcript in Hepatitis B virus-related HCC. All results in this article were analyzed with the software SPSS version 19.0 for Windows, and different groups were compared by χ2 test as ***: Based on the data from COSMIC database, compared with other solid tumors, mutation frequency of CTNNB1 was significantly higher in HCC(P ding to the COSMIC database, TP53 GOF mutation is rare in HCC, which indicates that TP53 GOF mutation is not a reason for the aberrant activation of Wnt signaling in HCC. APC and AXIN1 were mutated in HCC. By using methylation sensitive restriction enzym

关键词： β-catenin CTNNB1 Hepatitis B virus Hepatocellular carcinoma TP53 Wnt signaling

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Antiviral therapies for hepatitis B virus-related hepatocellular carcinoma

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World Journal of Gastroenterology 2015年第13期21卷 3860-3866页

Chronic hepatitis B virus(HBV) infection is a critical risk factor for the carcinogenesis and progression of hepatocellular carcinoma(HCC). It promotes HCC development by inducing liver fibrogenesis, genetic and epigenetic alterations, and the expression of active viral-coded proteins. Effective antiviral treatments inhibit the replication of HBV, reduce serum viral load and accelerate hepatitis B e antigen serum conversion. Timely initiation of antiviral treatment is not only essential for preventing the incidence of HCC in chronic hepatitis B patients, but also important for reducing HBV reactivation, improving liver function, reducing or delaying HCC recurrence, and prolonging overall survival of HBV-related HCC patients after curative and palliative therapies. The selection of antiviral drugs, monitoring of indicators such as HBV DNA and hepatitis B surface antigen, and timely rescue treatment when necessary, are essential in antiviral therapies for HBVrelated HCC.

关键词： Chronic hepatitis B Hepatocellular carcinoma Antiv

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Dual gRNAs guided CRISPR/Cas9 system inhibits hepatitis B virus replication

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World Journal of Gastroenterology 2015年第32期21卷 9554-9565页

作者： Jie Wang Zhong-Wei Xu Shuang Liu Rui-Yang Zhang shan-long ding Xiao-Meng Xie Lu long Xiang-Mei Chen Hui Zhuang Feng-Min Lu State Key Laboratory of Natural and Biomimetic Drugs Department of Microbiology and Infectious Disease Center School of Basic Medicine Peking University Health Science Center Department of Gastroenterology Pennsylvania Hospital University of Pennsylvania Beijing Artificial Liver Treatment and Training Center Beijing Youan Hospital Capital Medical University Department of Epidemiology and Biostatistics College of Public Health Zhengzhou University

AIM: To screen and investigate the effective g RNAs against hepatitis B virus(HBV) of genotypes ***: A total of 15 g RNAs against HBV of genotypes A-D were designed. Eleven combinations of two above g RNAs(dual-g RNAs) covering the regulatory region of HBV were chosen. The efficiency of each g RNA and 11 dual-g RNAs on the suppression of HBV(genotypes A-D) replication was examined by the measurement of HBV surface antigen(HBs Ag) or e antigen(HBe Ag) in the culture supernatant. The destruction of HBV-expressing vector was examined in Hu H7 cells co-transfected with dual-g RNAs and HBVexpressing vector using polymerase chain reaction(PCR) and sequencing method, and the destruction of ccc DNAwas examined in Hep AD38 cells using KCl precipitation, plasmid-safe ATP-dependent DNase(PSAD) digestion, rolling circle amplification and quantitative PCR combined method. The cytotoxicity of these g RNAs was assessed by a mitochondrial tetrazolium ***: All of g RNAs could significantly reduce HBs Ag or HBe Ag production in the culture supernatant, which was dependent on the region in which g RNA against. All of dual g RNAs could efficiently suppress HBs Ag and/or HBe Ag production for HBV of genotypes A-D, and the efficacy of dual g RNAs in suppressing HBs Ag and/or HBe Ag production was significantly increased when compared to the single g RNA used alone. Furthermore, by PCR direct sequencing we confirmed that these dual g RNAs could specifically destroy HBV expressing template by removing the fragment between the cleavage sites of the two used g RNAs. Most importantly, g RNA-5 and g RNA-12 combination not only could efficiently suppressing HBs Ag and/or HBe Ag production, but also destroy the ccc DNA reservoirs in Hep AD38 ***: These results suggested that CRISPR/Cas9 system could efficiently destroy HBV expressing templates(genotypes A-D) without apparent cytotoxicity. It may be a potential approach for eradication of persistent HBV ccc DNA in c

关键词： Dual g RNAs CRISPR/Cas9 Hepatitis B ccc DNA Antivi

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单个透空四面体水槽试验

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水运工程 2017年第2期 83-88,93页

作者：王倩芸储昊朱云飞单鼎隆重庆交通大学河海学院国家内河航道整治工程技术研究中心重庆400074

针对透水框架附近的流场分布规律的相关问题,通过采用高精度河流动力学水槽试验,利用基于变频技术的DCMS控制系统,进行单个透空四面体模型试验。试验在透空四面体周围布置972个测点,每个测点采用旋桨式光电流速仪测量。通过与无框架流... 详细信息

针对透水框架附近的流场分布规律的相关问题,通过采用高精度河流动力学水槽试验,利用基于变频技术的DCMS控制系统,进行单个透空四面体模型试验。试验在透空四面体周围布置972个测点,每个测点采用旋桨式光电流速仪测量。通过与无框架流场的对比,分析横断面流速沿垂直水流方向和沿高度方向的变化规律,得到透空四面体周围流速分布规律和具体减速、增速部位、平均减速率,揭示了透空四面体对水流扰动作用。

关键词：透空四面体减速效果减速部位平均减速率

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A Flexible Smart Healthcare Platform Conjugated with Artificial Epidermis Assembled by Three‑Dimensionally Conductive MOF Network for Gas and Pressure Sensing

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Nano-Micro Letters 2025年第2期17卷 601-620页

作者： Qingqing Zhou Qihang ding Zixun Geng Chencheng Hu long Yang Zitong Kan Biao Dong Miae Won Hongwei Song Lin Xu Jong Seung Kim State Key Laboratory of Integrated Optoelectronics College of Electronic Science and EngineeringJilin UniversityChangchun 130012People’s Republic of China Department of Chemistry Korea UniversitySeoul 02841Republic of Korea TheranoChem Incorporation Seoul 02856Republic of Korea

The rising flexible and intelligent electronics greatly facilitate the noninvasive and timely tracking of physiological information in telemedicine *** building bionic-sensitive moieties is vital for designing efficient electronic skin with advanced cognitive functionalities to pluralistically capture external ***,realistic mimesis,both in the skin’s three-dimensional interlocked hierarchical structures and synchronous encoding multistimuli information capacities,remains a challenging yet vital need for simplifying the design of flexible logic ***,we construct an artificial epidermal device by in situ growing Cu_(3)(HHTP)_(2) particles onto the hollow spherical Ti_(3)C_(2)T_(x) surface,aiming to concurrently emulate the spinous and granular layers of the skin’s *** bionic Ti_(3)C_(2)T_(x)@Cu_(3)(HHTP)_(2) exhibits independent NO_(2) and pressure response,as well as novel functionalities such as acoustic signature perception and Morse code-encrypted message ***,a wearable alarming system with a mobile application terminal is self-developed by integrating the bimodular senor into flexible printed *** system can assess risk factors related with asthmatic,such as stimulation of external NO_(2) gas,abnormal expiratory behavior and exertion degrees of fingers,achieving a recognition accuracy of 97.6%as assisted by a machine learning *** work provides a feasible routine to develop intelligent multifunctional healthcare equipment for burgeoning transformative telemedicine diagnosis.

关键词： Ti_(3)C_(2)T_(x)@Cu_(3)(HHTP)_(2)composites NO_(2)/pressure flexible sensors Health-monitoring Machine learning

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Rock fracture mechanism of buffer blasting with cushion layer at the borehole bottom

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 325-334页

作者： Xinguang Zhu Chenxi ding Zhe Sui Hong Su Xu Guo School of Civil and Resources Engineering University of Science and Technology BeijingBeijing 100083China Key Laboratory for Mechanics in Fluid Solid Coupling Systems Institute of MechanicsChinese Academy of SciencesBeijing 100190China School of Mechanics and Civil Engineering China University of Mining and Technology(Beijing)Beijing 100083China School of Chemical and Blasting Engineering Anhui University of Science and TechnologyHuainan 232001China

This study primarily investigates the rock fracture mechanism of bottom cushion layer blasting and explores the effects of the bottom cushion layer on rock *** involves analyses of the evolution patterns of blasting stress,characteristics of crack distribution,and rock fracture features in the ***,blasting model experiments were carried out using the dynamic caustics principle to investigate the influence of bottom cushion layers and initiation methods on the integrity of the bottom rock *** experimental results indicate that the combined use of bottom cushion layers and inverse initiation effectively protects the integrity of the bottom rock ***,the process of stress wave propagation and dynamic crack propagation in rocks was simulated using the continuum-discontinuum element method(CDEM)and the Landau explosion source model,with varying thicknesses of bottom cushion *** numerical simulation results indicate that with increasing cushion thickness,the absorption of energy generated by the explosion becomes more pronounced,resulting in fewer cracks in the bottom rock *** illustrates the positive role of the cushion layer in protecting the integrity of the bottom rock mass.

关键词： bottom cushion layer blasting crack propagation continuum-discontinuum element method dynamic stress intensity factor

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Enhanced post-combustion CO2 capture and direct air capture by plasma surface functionalization of graphene adsorbent

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Journal of Energy Chemistry 2025年第1期100卷 653-664页

作者： Rahul Navik Eryu Wang Xiao ding Huang Yunyi Yiyu Liu Jia Li Guangdong-Hong Kong Joint Laboratory for Carbon Neutrality Jiangmen Laboratory of Carbon and Science and Technology Guangzhou HKUST Fok Ying Tung Research Institute The Hong Kong University of Science and Technology (Guangzhou) Carbon Neutrality and Climate Change Thrust Society Hub The Hong Kong University of Science and Technology (Guangzhou) Stoddart Institute of Molecular Science Department of Chemistry Zhejiang University

Graphene has enormous potential to capture CO2due to its unique properties and ***,graphene-based adsorbents have drawbacks of lower CO2adsorption capacity and poor *** work demonstrates a one-step rapid and sustainable N2/H2plasma treatment process to prepare graphene-based sorbent material with enhanced CO2adsorption *** treatment directly enriches amine species,increases surface area,and improves textural *** CO2adsorption capacity increases from 1.6 to 3.3 mmol/g for capturing flue gas,and from 0.14 to 1.3 mmol/g for direct air capture （DAC）.Importantly,the electrothermal property of the plasma-modified aerogels has been significantly improved,resulting in faster heating rates and significantly reducing energy consumption compared to conventional external heating for regeneration of *** aerogels display improved selectivity of 42 and 87 after plasma modification for 5 and 10 min,*** plasma-treated aerogels display minimal loss between 17%and 19% in capacity after 40 adsorption/desorption cycles,rendering excellent *** N2/H2plasma treatment of adsorbent materials would lower energy expenses and prevent negative effects on the global economy caused by climate change.

关键词： Carbon neutrality CO 2 capture Climate change Plasma treatment Graphene aerogel

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空气悬架系统的理想孪生动挠度跟踪控制策略

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西安交通大学学报 2025年第1期59卷 159-171页

作者：赵雷雷张浩于曰伟甄冉丁钒单溪玉丁家和山东理工大学交通与车辆工程学院山东淄博255000 山东安博机械科技股份有限公司研发部山东淄博255000 山东美晨工业集团有限公司研发中心山东潍坊262200

为解决车辆主动悬架系统减振效果与控制能耗间的矛盾,基于“数字孪生、以虚控实、节能减振”理念,提出了主动空气悬架系统的理想孪生动挠度跟踪控制策略。首先,以车辆座椅主动空气悬架系统为例,利用准零刚度系统构建了真实空气悬架系统... 详细信息

为解决车辆主动悬架系统减振效果与控制能耗间的矛盾,基于“数字孪生、以虚控实、节能减振”理念,提出了主动空气悬架系统的理想孪生动挠度跟踪控制策略。首先,以车辆座椅主动空气悬架系统为例,利用准零刚度系统构建了真实空气悬架系统的理想数字孪生体,同时建立了该孪生体的参数匹配设计方法;然后,以空气弹簧气体压力控制为节点,设计了外环位置模糊非奇异快速终端滑模控制器,以及考虑空气弹簧充放气过程中电磁阀非线性流量特性的内环气压控制器,进而实现真实悬架系统对实际激励下孪生体动挠度的跟踪;最后,通过台架模拟实验与整车平顺性仿真实验,对所提控制策略的有效性与先进性进行了验证。结果表明:与以降低加速度为控制目标的传统控制策略相比,所提策略在随机路况下的座椅垂向振动加速度均方根更小且控制能耗均方根最小降幅达65.0%,冲击路况下座椅垂向振动剂量也更小且主动控制力最大降幅高达45.5%。所提策略性能明显优于传统策略,能在有效提高悬架系统减振性能的同时大幅降低主动控制能耗,有效解决了上述矛盾,可推广应用于各类主动悬架系统,为节能减振提供新的研究思路。

关键词：车辆空气悬架节能减振理想孪生动挠度

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From waste to wealth:Coal tar residue derived carbon materials as low-cost anodes for potassium-ion batteries

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 464-475页

作者： Zhonghua Lu Jun Shen Xin Zhang Lingcong Chao Liang Chen ding Zhang Tao Wei Shoudong Xu College of Chemical Engineering and Technology Taiyuan University of TechnologyTaiyuan 030024China College of Chemistry Taiyuan University of TechnologyTaiyuan 030024China School of Chemical Engineering and Pharmacy Wuhan Institute of TechnologyWuhan 430205China School of Energy and Power Jiangsu University of Science and TechnologyZhenjiang 212003China

Carbon materials are widely recognized as highly promising electrode materials for various energy storage system *** tar residues(CTR),as a type of carbon-rich solid waste with high value-added utilization,are crucially important for the development of a more sustainable *** this study,we employed a straightforward direct carbonization method within the temperature range of 700-1000℃to convert the worthless solid waste CTR into economically valuable carbon materials as anodes for potassium-ion batteries(PIBs).The effect of carbonization temperature on the microstructure and the potassium ions storage properties of CTR-derived carbons(CTRCs)were systematically explored by structural and morphological characterization,alongside electrochemical performances *** on the co-regulation between the turbine layers,crystal structure,pore structure,functional groups,and electrical conductivity of CTR-derived carbon carbonized at 900℃(CTRC-900H),the electrode material with high reversible capacity of 265.6m Ah·g^(-1)at 50 m A·g^(-1),a desirable cycling stability with 93.8%capacity retention even after 100 cycles,and the remarkable rate performance for PIBs were ***,cyclic voltammetry(CV)at different scan rates and galvanostatic intermittent titration technique(GITT)have been employed to explore the potassium ions storage mechanism and electrochemical kinetics of *** indicate that the electrode behavior is predominantly governed by surface-induced capacitive processes,particularly under high current densities,with the potassium storage mechanism characterized by an“adsorption-weak intercalation”*** work highlights the potential of CTR-based carbon as a promising electrode material category suitable for high-performance PIBs electrodes,while also provides valuable insights into the new avenues for the high value-added utilization of CTR.

关键词： coal tar residue carbon materials anode potassium-ion batteries high value-added

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Discrimination of Transgenic Rice Based on Near Infrared Reflectance Spectroscopy and Partial Least Squares Regression Discriminant Analysis

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Rice science 2015年第5期22卷 245-249页

作者： ZHANG long WANG shan-shan ding Yan-fei PAN Jia-rong ZHU Cheng College of Ecology Lishui University College of Life Sciences Zhejiang University College of Life Sciences China Jiliang University

Near infrared reflectance spectroscopy （NIRS）, a non-destructive measurement technique, was combined with partial least squares regression discrimiant analysis （PLS-DA） to discriminate the transgenic （TCTP and mi166） and wild type （Zhonghua 11） rice. Furthermore, rice lines transformed with protein gene （OsTCTP） and regulation gene （Osmi166） were also discriminated by the NIRS method. The performances of PLS-DA in spectral ranges of 4 000-8 000 cm-1 and 4 000-10 000 cm-1 were compared to obtain the optimal spectral range. As a result, the transgenic and wild type rice were distinguished from each other in the range of 4 000-10 000 cm-1, and the correct classification rate was 100.0% in the validation test. The transgenic rice TCTP and mi166 were also distinguished from each other in the range of 4 000-10 000 cm-1, and the correct classification rate was also 100.0%. In conclusion, NIRS combined with PLS-DA can be used for the discrimination of transgenic rice.

关键词： near infrared reflectance spectroscopy genetically-modified food regulation gene protein gene partial least squares regression discrimiant analysis

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