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Rapid identification of full-length genome and tracing variations of monkeypox virus in clinical specimens based on mNGS and amplicon sequencing

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Virologica Sinica 2024年第1期39卷 134-143页

作者： Changcheng Wu Ruhan A Sheng Ye Fei Ye Weibang Huo Roujian lu Yue Tang Jianwei Yang Xuehong Meng Yun Tang Shuang Chen Li Zhao Baoying Huang Zhongxian Zhang Yuda Chen Dongfang Li Wenling Wang Ke-jia shan Jian lu Wenjie Tan NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing100052China Chongqing Municipal Key Laboratory for High Pathogenic Microbes Chongqing Center for Disease Control and PreventionChongqing400042China MGI BGI-ShenzhenShenzhen518083China Thermo Fisher Scientific Beijing100013China School of Public Health Baotou Medical CollegeBaotou014030China BGI PathoGenesis Pharmaceutical Technology Shenzhen518000China State Key Laboratory of Protein and Plant Gene Research Center for BioinformaticsSchool of Life SciencesPeking UniversityBeijing100871China College of Life Sciences University of Chinese Academy of SciencesBeijing100049China

The monkeypox virus(MPXV)has triggered a current outbreak *** sequencing of MPXV and rapid tracing of genetic variants will benefit disease diagnosis and *** is a significant challenge but necessary to optimize the strategy and application of rapid full-length genome identification and to track variations of MPXV in clinical specimens with low viral loads,as it is one of the DNA viruses with the largest genome and the most AT-biased,and has a significant number of tandem *** we evaluated the performance of metagenomic and amplicon sequencing techniques,and three sequencing platforms in MPXV genome sequencing based on multiple clinical specimens of five mpox cases in Chinese *** rapidly identified the full-length genome of MPXV with the assembly of accurate tandem repeats in multiple clinical *** sequencing enables cost-effective and rapid sequencing of clinical specimens to obtain high-quality MPXV ***-generation sequencing facilitates the assembly of the terminal tandem repeat regions in the monkeypox virus genome and corrects a common misassembly in published ***,several intra-host single nucleotide variations were identified in the first imported mpox *** study offers an evaluation of various strategies aimed at identifying the complete genome of MPXV in clinical *** findings of this study will significantly enhance the surveillance of MPXV.

关键词： Monkeypox virus(MPXV) Metagenomic Next generation sequencing Amplicon Third-generation sequencing

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Gene mutations in newly diagnosed multiple myeloma patients detected by next-generation sequencing technology

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Cancer Pathogenesis and Therapy 2024年第3期2卷 205-211页

作者： Yutong Wang Mengzhen Wang Bin Chu Minqiu lu Lei Shi shan Gao Yuan Chen Qin Yan Na Ji Li Bao Department of Hematology Beijing Jishuitan HospitalCapital Medical UniversityBeijing 100035China

Background:Multiple myeloma(MM)is a heterogeneous plasma-derived hematopoietic malignancy with complex genetic mutation contributing to the *** gene sequencing has been applied in MM,genetic features from Chinese MM patients are reported *** investigated the genetic mutation of newly diagnosed multiple myeloma(NDMM)patients and explore its correlation with cytogenetic abnormalities detected by fluorescence in situ hybridization(FISH).Methods:A total of 206 patients with NDMM were *** enriching plasma cells with CD138 magnetic beads,92 MM-related target gene mutations were detected by the Illumina sequencing platform,and six common genetic abnormalities were detected by ***:162 cases(78.6%)had at least one gene mutation detected by *** top 5 mutated genes were KRAS,NRAS,TRAF3,BRAF,and *** abnormalities detected by FISH have a certain correlation with gene mutations,t(11;14)translocations are often accompanied by CCND1 and TP53 mutations,KLHL6 in t(4;14),SP140,CDKN1B and PRKD2 in t(14;16)and t(14;20)*** mutation ratio was higher for EGR1,while lower of CCND1 in patients with gain *** TP53 mutation was more likely in patients with 17p *** gene mutation affects the pathway of the RNA process is more frequently occurring in males and age less than 70 years *** International Staging System(ISS)Stage Ⅲ correlated with gene mutations in the NK-κB pathway while Revised ISS(R-ISS)Stage Ⅲ correlated with the DNA damage repair ***:There are various gene mutations in NDMM patients,mainly RAS/MAPK and NF-κB pathway gene pathways.

关键词： Multiple myeloma Next-generation sequencing Genetics mutation

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Trade-offs between the accumulation of cuticular wax and jasmonic acid-mediated herbivory resistance in maize

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Journal of Integrative Plant Biology 2024年第1期66卷 143-159页

作者： Jiong Liu lu Li Zhilong Xiong Christelle AMRobert Baozhu Li shan He Wenjie Chen Jiasheng Bi Guanqing Zhai Siyi Guo Hui Zhang Jieping Li Shutang Zhou Xi Zhang Chun‐Peng Song School of Life Sciences Henan UniversityKaifeng 475004China State Key Laboratory of Crop Stress Adaptation and Improvement Henan UniversityKaifeng 475004China State Key Laboratory of Cotton Bio‐Breeding and Integrated Utilization Henan UniversityKaifeng 475004China Sanya Institute of Henan University Sanya 572025China Institute of Plant Sciences University of BernBern 3013Switzerland

Plants have evolved complex physical and chemical defense systems that allow them to withstand herbivory *** of a complex mixture of very-long-chain fatty acids(VLCFAs)and their derivatives,cuticular wax constitutes the first physical line of defense against ***,we report the function of Glossy 8(ZmGL8),which encodes a 3-ketoacyl reductase belonging to the fatty acid elongase complex,in orchestrating wax production and jasmonic acid(JA)-mediated defenses against herbivores in maize(Zea mays).The mutation of GL8 enhanced chemical defenses by activating the JA-dependent *** observed a trade-off between wax accumulation and JA levels across maize glossy mutants and 24 globally collected maize inbred *** addition,we demonstrated that mutants defective in cuticular wax biosynthesis in Arabidopsis thaliana and maize exhibit enhanced chemical *** transcriptomic and lipidomic analyses indicated that the gl8 mutant confers chemical resistance to herbivores by remodeling VLCFA-related lipid metabolism and subsequent JA biosynthesis and *** results suggest that VLCFA-related lipid metabolism has a critical role in regulating the trade-offs between cuticular wax and JA-mediated chemical defenses.

关键词： cuticular wax fall armyworm herbivore resistance jasmonic acid maize plant-herbivore interactions

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Ziwan-Taoren herb pair can exert an therapeutical effect in primary Sjogren’s syndrome through inhibiting the TLR/NF-κB pathway

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Traditional Medicine Research 2024年第8期9卷 11-22页

作者： Kuok-Tong Lei Yun-Xia Wu Yun lu Zi-shan Wang Thi-Huong Nguyen Qiu-Ying Cai Wen Zhu Yue Wang First Clinical Medical College Nanjing University of Chinese MedicineNanjing 210000China Faculty of Traditional Medicine Phenikaa UniversityHa Noi 100803Vietnam Department of Rheumatology and Immunology Affiliated Hospital of Nanjing University of Chinese MedicineNanjing 210000China

Background:Ziwan and Taoren(ZT)is a classic medicine pair in the formula of Mai Dong Di Shao Decoction,has been used to treat primary Sjogren’s syndrome(pSS)for more than 20 *** its action mechanism is still *** study is aimed to reveal the potential mechanism of ZT treated pSS and discover its active compounds of ZT and therapeutic target for ***:Firstly,the potential pathways of ZT for pSS treatment were predicted through network pharmacology and GO and KEGG enrichment ***,the inter-structural relationships between active compounds of ZT and target proteins were visualized using molecular docking ***,efficacy and mechanism were conducted through in vivo experiments,such as water intake,spleen index,hematoxylin-eosin staining pathological changes,ELISA,Western Blot analysis,and immunofluorescence ***:Nine active compounds were extracted from network pharmacology,including quercitrin,luteolin,kaempferol,β-sitosterol,isorhamnetin,galangin,hederagenin,diosmetin and gibberellin *** disease targets were identified:RELA,TP53,AKT1,interleukin(IL)6,MAPK1,ESR1,IL10;with RELA being the most core *** and GO enrichment analysis indicated that ZT may act through the TLR/NF-κB/RELA inflammatory mechanism *** results of molecular docking showed that ZT’s active compounds bind well to the RELA(p65)*** vivo results demonstrated that a high dose of ZT significantly improved water intake and reduced lymphocytes infiltration in submandibular gland pathology in NOD *** expression content of AQP5 and vasoactive intestinal peptide in the submaxillary gland was significantly increased,while levels of inflammatory factors such as tumor necrosis factor-α,IL-6,and IL-1βalong with protein expressions including toll-like receptor4,p-p65 and p-IKKα/βin NF-κB pathway were ***:The ZT treatment exhibits a promising efficacy in mitigating dryness symptoms of pSS,potentially attr

关键词： traditional Chinese medicine primary Sjogren’s syndrome NF-κB RELA

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Side-Chain-Type Polyimide-Cu Complexes with Suppressed Activation Energy of Relaxation for Advanced High-Temperature Capacitor

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Chinese Journal of Polymer Science 2024年第8期42卷 1038-1048页

作者： Bai-Yang Zhou Ze-Tong Ma Shi-Long Zhong Zhen-Jie Huang Qi Guo Deng-Chong Feng Zhong-Ke Yuan Shao-Lin lu Yu-Zhao Yang Cheng Wang Ding-shan Yu Xu-Dong Chen Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education Guangdong Engineering Technology Research Centre for High Performance Organic and Polymer Photoelectric Functional FilmsSchool of ChemistrySun Yat-sen UniversityGuangzhou 510275China School of Chemical Engineering and Light Industry Guangdong University of TechnologyGuangzhou 510006China Guangdong Provincial Laboratory of Chemistry and Fine Chemical Industry Jieyang Centre Jieyang 515200China Chemistry and Chemical Engineering Guangdong Laboratory Shantou 515031China

The burgeoning growth of the new energy vehicles and aviation industry has escalated the need for energy storage capacitors capable of stable operation in harsh *** advent of metal-polyimide complexes has illuminated a novel approach for preparing tem perature-resistant ***,the general application of these metal-polyimide com plexes is impeded by the high dielectric loss and low breakdown strength,consequences of main-chain coordination and excessive metal ions ***,our study proposes a novel polyimideCu complex material(POP-Cu)predicated on side-chain-type pyridine-Cu coordination,utilizing the structural backbone PMDA-ODA of mature commercial PI(Kapton)with reliable *** to the high degree of freedom afforded by the side chain with suppressed relaxation activation energy and the long-range electron delocalization formed by d-πcoordination,the dielectric constant of this material containing merely 2.7 mol%Cu increases from 3.25(POPI)to 5.58,while maintaining a remarkably low dielectric loss of ***,this material exhibits a substantial DC breakdown strength of 436.2 MV·m^(-1)and a high energy density of 5.42 J·cm^(-3),coupled with superior mechanical and thermal *** at 150℃,it retains over 90%of its room-temperature energy density,demon strating notable dielectric stability under high *** attributes underscore its promising application for capacitors operating in harsh environments.

关键词： Dielectric performance Polyimide complexes Side-Chain-Type High-temperature capacitor

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Improvement of the nutritional support management system for patients in intensive care units

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World Journal of Psychiatry 2024年第1期14卷 44-52页

作者： Yuan-Yuan Zhang Chun-Yi Wang Dong-Xian Guo Hai-Nu Gao Xian-shan Jin Yan-Li Wu lu-Han Chen Zhi-Xian Feng Department of Critical Care Medicine Shulan(Hangzhou)HospitalZhejiang Shuren University Shulan International Medical CollegeHangzhou 310022Zhejiang ProvinceChina Department of Information Shulan(Hangzhou)HospitalZhejiang Shuren University Shulan International Medical CollegeHangzhou 310022Zhejiang ProvinceChina Department of Pharmacy Shulan(Hangzhou)HospitalZhejiang Shuren University Shulan International Medical CollegeHangzhou 310022Zhejiang ProvinceChina Department of Nutrition Shulan(Hangzhou)HospitalZhejiang Shuren University Shulan International Medical CollegeHangzhou 310022Zhejiang ProvinceChina Administrative Office Shulan(Hangzhou)HospitalZhejiang Shuren University Shulan International Medical CollegeHangzhou 310022Zhejiang ProvinceChina

BACKGROUND Nutritional support for patients hospitalized in the intensive care unit(ICU)is an important part of clinical treatment and care,but there are significant implementation *** To introduce a modified nutritional support management system for ICU patients based on closed-loop information management and psychological *** The division of functions,personnel training,system construction,development of an intelligent decision-making software system,quality control,and improvement of the whole process were carried out to systematically manage nutritional support for ICU *** Following the implementation of the whole process management system,the scores of ICU medical staff’s knowledge,attitudes/beliefs,and practices regarding nutritional support were comprehensively *** proportion of hospital bed-days of total enteral nutrition(EN)in ICU patients increased from 5.58%to 11.46%,and the proportion of EN plus parenteral nutrition increased from 42.71%to 47.07%.The rate of EN initiation within 48 h of ICU admission increased from 37.50%to 48.28%,and the EN compliance rate within 72 h elevated from 20.59%to 31.72%.After the implementation of the project,the Self-rating Anxiety Scale score decreased from 61.07±9.91 points to 52.03±9.02 points,the Self-rating Depression Scale score reduced from 62.47±10.50 points to 56.34±9.83 points,and the ICU stay decreased from 5.76±2.77 d to 5.10±2.12 *** The nutritional support management system based on closed-loop information management and psychological counseling achieved remarkable results in clinical applications in ICU patients.

关键词： Closed-loop information Psychological counseling Intensive care unit patients Nutritional support Management system

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The efficacy of a novel tomato extracts formulation on skin aging and pigmentation:A randomized,double-blind,parallel-controlled trial

Journal of dermatologic science and cosmetic technology

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Journal of dermatologic science and cosmetic technology 2024年第1期1卷 13-20页

作者： Silu Zhang shan lu Yun Wang Jiayi Ni Guoxun Xiao Wonderlab Innovation Centre for Healthcare Shenzhen Porshealth Bioengineering Co.Ltd.Shenzhen 518116China Department of Dermatology Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200125China Sprim(China)Consulting Co.Ltd. Shanghai 201201China

Background:Oxidative stress is a significant factor in skin aging and pigmentation,which can be precipitated by various *** and tyrosinase inhibitors,such as carotenoids,yeast extract(glutathione),sodium hyaluronate,astaxanthin,and niacin,can individually protect the skin against aging through distinct *** mechanisms potentially enhance the skin barrier and improve signs of aging and ***,the synergistic effects of these compounds,as found in a golden tomato extract formulation,have been scarcely ***:To evaluate the effects of an orally administered formulation on the skin aging and *** and Methods:In this study,a randomized,double-blind,parallel-controlled trial was conducted,utilizing the WONDERLAB?Tomato Niacinamide *** of all participants,62 volunteers completed the experiment and were included in the statistical ***:The results indicated that after eight weeks of consuming the research product,there were no significant changes in the skin indicators within the placebo *** contrast,the treatment group receiving the sample formulation exhibited a 35.63%increase in stratum corneum hydration and a 29.39%reduction in transepidermal water loss(TEWL),suggesting enhanced skin *** assessments revealed improvements in skin color and gloss index by 15.03%and 11.41%,respectively,in the treatment ***,the skin gloss and individual typology angle(ITA)value increased by 18.59%and 6.36%,respectively,leading to a lighter skin *** enhancements were also observed in skin pigmentation,color uniformity,and *** eight weeks of intervention with the sample,blood levels of superoxide dismutase(SOD)and glutathione peroxidase(GPx)increased,while malondialdehyde(MDA)levels ***:These findings confirm that continuous intake of the tomato extract formulation over eight weeks effectively improved the volunteers'skin w

关键词： Skin whitening Skin aging Antioxidant Gold tomato extracts Combination effect

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Incidence and warning signs for complications of human brucellosis: a multi-center observational study from China

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Infectious Diseases of Poverty 2024年第1期13卷 53-62页

作者： Qing-Nan Shi Hui-Jie Qin Qiao-shan lu hu Li Zhong-Fa Tao Meng-Guang Fan Mu-Heta Aishan Zeng-Qiang Kou Qiu-Lan Chen Wen-Wu Yin Yan-Ping Zhang National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases Chinese Center for Disease Control and PreventionChangbai RoadChangping DistrictBejing 102206China School of Public Health Guangxi Medical UniversityNanningChina Nanning Center for Disease Control and Prevention NanningChina Guizhou Center for Disease Control and Prevention GuiyangChina Inner Mongolia Center for Disease Control and Prevention HohhotChina Xinjiang Uighur Autonomous Region Center for Disease Control and Prevention UrumqiChina shandong Center for Disease Control and Prevention JinanChina

Background Brucellosis is a severe zoonotic disease that is often overlooked, particularly in impoverished countries. Timely identification of focal complications in brucellosis is crucial for improving treatment outcomes. However, there is currently a lack of established indicators or biomarkers for diagnosing these complications. Therefore, this study aimed to investigate potential warning signs of focal complications in human brucellosis, with the goal of providing practical parameters for clinicians to aid in the diagnosis and management of *** A multi-center cross-sectional study was conducted in China from December 2019 to August 2021. The study aimed to investigate the clinical characteristics and complications of patients with brucellosis using a questionnaire survey and medical record system. The presence of warning signs for complications was assessed using univariate and multivariate logistic regression models. Receiver operating characteristic (ROC) curves and the area under the curve (AUC) were used for variable screening and model *** A total of 880 participants diagnosed with human brucellosis were enrolled. The median age of the patients was 50 years [interquartile range (IQR): 41.5-58.0], and 54.8% had complications. The most common organ system affected by complications was the osteoarticular system (43.1%), with peripheral arthritis (30.0%), spondylitis (16.6%), paravertebral abscess (5.0%), and sacroiliitis (2.7%) being the most prevalent. Complications in other organ systems included the genitourinary system (4.7%), respiratory system (4.7%), and hematologic system (4.6%). Several factors were found to be associated with focal brucellosis. These factors included a long delay in diagnosis [odds ratio (OR) = 3.963, 95% confidence interval(CI): 1.906-8.238 for > 90 days], the presence of underlying disease (OR = 1.675, 95%CI: 1.176-2.384), arthralgia (OR = 3.197, 95%CI: 1.986-5.148), eye bulging pain (OR = 3.482, 95%CI:

关键词： Brucellosis Complications Clinical spectrum Warning signs

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Development of a droplet digital polymerase chain reaction assay for the sensitive detection of total and integrated HIV-1 DNA

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Chinese Medical Journal 2024年第6期137卷 729-736页

作者： Lin Yuan Zhiying Liu Xin Zhang Feili Wei shan Guo Na Guo Lifeng Liu Zhenglai Ma Yunxia Ji Rui Wang Xiaofan lu Zhen Li Wei Xia Hao Wu Tong Zhang Bin Su Beijing Key Laboratory for HIV/AIDS Research Clinical and Research Center for Infectious DiseasesBeijing Youan HospitalCapital Medical UniversityBeijing 100069China Beijing Institute of Hepatology Beijing Youan HospitalCapital Medical UniversityBeijing 100069China

Background:Total human immunodeficiency virus(HIV)DNA and integrated HIV DNA are widely used markers of HIV *** digital polymerase chain reaction(ddPCR)can be used for absolute quantification without needing a standard ***,we developed duplex ddPCR assays to detect and quantify total HIV DNA and integrated HIV ***:The limit of detection,dynamic ranges,sensitivity,and reproducibility were evaluated by plasmid constructs containing both the HIV long terminal repeat(LTR)and human CD3 gene(for total HIV DNA)and ACH-2 cells(for integrated HIV DNA).Forty-two cases on stable suppressive antiretroviral therapy(ART)were assayed in total HIV DNA and integrated HIV *** coefficient analysis was performed on the data related to DNA copies and cluster of differentiation 4 positive(CD4^(+))T-cell counts,CD8^(+)T-cell counts and CD4/CD8 T-cell ratio,*** assay linear dynamic range and lower limit of detection(LLOD)were also ***:The assay could detect the presence of HIV-1 copies 100%at concentrations of 6.3 copies/reaction,and the estimated LLOD of the ddPCR assay was 4.4 HIV DNA copies/reaction(95%confidence intervals[CI]:3.6-6.5 copies/reaction)with linearity over a 5-log_(10)-unit range in total HIV DNA *** the integrated HIV DNA assay,the LLOD was 8.0 copies/reaction(95%CI:5.8-16.6 copies/reaction)with linearity over a 3-log 10-unit *** HIV DNA in CD4^(+)T cells was positively associated with integrated HIV DNA(r=0.76,P<0.0001).Meanwhile,both total HIV DNA and integrated HIV DNA in CD4^(+)T cells were inversely correlated with the ratio of CD4/CD8 but positively correlated with the CD8^(+)T-cell ***:This ddPCR assay can quantify total HIV DNA and integrated HIV DNA efficiently with robustness and *** can be readily adapted for measuring HIV DNA with non-B clades,and it could be beneficial for testing in clinical trials.

关键词： Human immunodeficiency virus HIV Integrated HIV-1 DNA Total HIV DNA Droplet digital polymerase chain reaction HIV reservoir Antiretroviral therapy

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Cytisine N-methylene-(5，7，4’-trihydroxy)-isoflavone induces neural stem cell proliferation and reverses cognitive deficits in early Alzheimer's disease models via canonical Wnt/β-catenin pathway

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Food Science and Human Wellness 2024年

作者： Yang-Yang lu Xiao-Wan Li Zhi-Ping Qin Wen-Jing Sun Yong-Biao Li Shao-Bo Liu lu lu Rong-Fei Yang Na-Na Zhang Yi-Ting Liu Jun Zhang Qing-shan Liu Key Laboratory of Ethnomedicine for Ministry of Education， Center on Translational Neuroscience， Minzu University of China Center for Life Sciences， School of Life Science and Technology， Harbin Institute of Technology Department of Ophthalmology， shandong Provincial Hospital Affiliated to shandong First Medical University

Alzheimer’s disease (AD) is a degenerative disease of the central nervous system. It results in cognitive dysfunction due to the loss of functional neurons and a deficit of new neurons， which can lead to death in severe cases. Repairing damaged neurons by promoting hippocampal neurogenesis has become therapeutic modality to combat neurodegenerative diseases. The novel isoflavone alkaloid LY01， found in the edible fruits of Sophora alopecuroides L.， has various neuroprotective effects. However， the molecular mechanism by which it promotes the proliferation of endogenous neural stem cells (NSCs) is not yet precisely known. The effects of LY01 were investigated in vivo and in vitro. In vivo experiments showed that LY01 could counteract the toxic damage of Aβ， enhance the learning ability and memory capacity of 5×FAD mice， and improve the morphology of neurons in the hippocampal region. In vitro experiments showed that LY01 was effective against antioxidant damage， improved the cell morphology of C17.2 mouse NSCs after hydrogen peroxide injury， and increased cell viability. Both in vitro and in vivo experiments promoted NSC proliferation by upregulating the mRNA and protein levels of the critical genes of the Wnt/β-catenin pathway， which increased levels of doublecortin to facilitate new neuronal generation. This indicates that the ability of LY01 to counteract Aβ toxicity and alleviate oxidative damage in early AD is associated with activation of the Wnt/β-catenin signaling pathway to promote NSC proliferation and thus repair damaged neurons.

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