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Vitamin D Insufficiency and Its Association with Biochemical and Anthropometric Variables of Young Children in Rural Southwestern China

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Chinese medical Journal 2016年第11期129卷 1273-1279页

作者： shan-shan Geng Jing-Qiu ma shan-shan Liu Jie Zhang Xiao-Yang Sheng Department of Children and Adolescents Health Care Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200092 China MOE-shanghai Key Laboratory of Children's Environmental Health Shanghai Institute for Pediatric Research Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200092 China Department of Anesthesia The Children's Hospital School of Medicine Zhejiang University School of Medicine Hangzhou Zhejiang 310003 China

Background： With recognition of the important roles of Vitamin D （VitD） in various physiological processes, increasing attention has been drawn to the status of VitD in early life. However, the VitD status of young children and the related factors in rural areas of Southwestern China remain unclear. This study aimed to explore VitD status and its seasonal variation in 18-month-old children living in rural Southwestern China. The association of VitD with biochemical and anthropometric variables was also investigated. Methods： A total of 177 18-month-old children in a rural area of Yunnan Province, Southwestern China, were enrolled. Serum concentrations of 25-hydroxy Vitamin D （25（OH）D） were measured through high-performance liquid chromatogram-tandem mass spectrometry. Parathyroid hormone （PTH） levels were measured with a chemiluminescence assay. Serum concentrations of calcium, phosphorus, and alkaline phosphatase （ALP） were also measured. Anthropometric data and the outdoor activity time of each participant were collected. Results： The serum 25（OH）D concentration was 26.61 ±7.26 ng/ml; concentrations lower than 30 ng/ml accounted for 70.6% of the participants and concentrations lower than 20 ng/ml accounted for 16.4%. The level of serum 25（OH）D was not significantly different among four seasons （P 〉 0.05）. A positive relationship was found between 25（OH）D concentration and the time of outdoor activities （r = 0.168, P 〈 0.05）. Serum PTH concentration was negatively correlated with 25（OH）D concentration （r - 0.163, P 〈 0.05）. A positive relationship was found between the serum concentrations of 25（OH）D and calcium （r = 0.154, P 〈 0.05）. No significant association was observed between 25（OH）D and ALP, phosphorus, or anthropometric variables. Conclusions： The prevalence of VitD insufficiency is high among young children in the rural Southwestern China regardless of the seasons. VitD supplementation is still essential to maintain VitD sufficiency for children living in

关键词： 25-Hydroxy Vitamin D Infant Rural Area Vitamin D Vitamin D Deficiency

Effect of β Radiation on Bcl-2 and Bax Expressions in Benign Prostate Hyperplasia Tissues

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Chemical Research in Chinese Universities 2008年第4期24卷 501-504页

作者： ma Qing-jie GAO Shi ZHAO Jie GU Xin-quan CAI shan-yu ZHAO Guo-qing Department of Nuclear Medicine China-Japan Union Hospital Jilin University Changchun 130033 P. R. China Isotope Division China Institute of Atomic Energy Beijing 102413 P. R. China

The authors chose specimens from nine normal prostate tissues（NP group）, 15 benign prostate hyperplasia（BPH） prostates（BPH group）, and 35 BPH prostates that had been treated＇with ^90Sr/^90Y Prostatic Hyperplasia Applicator（exposure group）. The expressions of bcl-2 and bax in stroma and epithelia of prostate tissues were demonstrated by means of immunohistochemical staining, and the staining positive rate was semiquantatively determined, so as to observe the expression of bcl-2 and bax genes in the prostate tissues of normal individuals and BPH patients, before and after fl radiation, and to evaluate the influence of fl radiation on bcl-2 and bax expressions. The expressions of gene bcl-2 in the prostate epithelia of NP and BPH are significantly higher than those in the prostate stroma（P〈0.01）. However, the expressions of bcl-2 in the prostate epithelia and stroma of the BPH group are obviously higher than those in the NP group（P〈0.01）. The expression of gene bax in the prostate epithelia of the NP group is higher than that in the BPH group（P〈0.05）. However, bcl-2 expressions in the prostate epithelia and stroma of the BPH group are significantly higher than the bax expressions（P〈0.01）. Compared with those of the NP group, the expressions of bcl-2 in the prostate epithelia and stroma of the exposure group decrease remarkably, even as the expressions of the bax notably increase（P〈0.01）. Thus, the administration of β radiation can remarkably affect bcl-2 and bax gene expressions, to regulate cell apoptosis, in the prostate tissues of BPH.

关键词： Benign prostate hyperplasia β Radiation Apoptosis

Effects of Charred Fructus Crataegi on the contractilily of isolated rat gastric and intestine muscle strips

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Effects of Charred Fructus Crataegi on the contractilily of ...

Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury

两岸三地药理学与临床药理学术会议

作者： ZHANG Hou-li DIAO Yun-peng LIU Zhi-hao HUANG shan-shan ma Xiao-chi LIN Vuan (College of pharmacy, Dalian medical university, Dalian 116044, China)

Objective The purpose of the study is to investigate the effects of Charred Fructus Crataegi Alco- hol Extract on contractililty of isolated rat gastric and intesting smooth muscle *** Isolated rat intestine was selected in the assay to test the effects of Charred Fructus Crataegi Alcohol Extract on contrac- tilty of isolated rat gastric and intestine smooth muscle strips using Krebs’ solution,to observe the effects of in the presence of acetylcholine or *** Charred Fructus Crataegi Alcohol Extract in the range of 2-8 mg crude drugs/mL could significantly reduce the contractility of rat gastric and intestine smooth muscle strips in a dose-dependent manner,and Charred Fructus Crataegi Alcohol Extract 8 mg·mL(crude drugs) could inhibit the stimulation induced by *** Fructus Crataegi Alcohol Extract 8 mg·mL(crude drugs) was found to have a inhibiton of the relaxtion concurrently used with ***- elusions The results suggest that Charred Fructus Crataegi Alcohol Extract has prominent inhibitory effects on the contractile activity of isolated rat gastric and intestine smooth muscle strips.

关键词： charred fructus crataegi gastric smooth muscle intestine smooth muscle contractilily

来源： cnki会议评论

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World Journal of Gastroenterology 2013年第4期19卷 492-502页

作者： Wei Liu Li-Ping shan Xue-Song Dong Xiao-Wei Liu Tao ma Zhi Liu Department of Emergency Medicine the First Affiliated Hospital of China Medical University Department of Urologic Surgery Shengjing HospitalChina Medical University

AIM:To study the effects of combined early fluid resuscitation and hydrogen inhalation on septic shockinduced lung and intestine ***:Wistar male rats were randomly divided into four groups:control group(Group A,n = 15);septic shock group(Group B,n = 15);early fluid resuscitation-treated septic shock group(Group C,n = 15);and early fluid resuscitation and inhalation of 2% hydrogentreated septic shock group(Group D,n = 15).The activity of hydroxyl radicals,myeloperoxidase(MPO),superoxide dismutase(SOD),diamine oxidase(DAO),and the concentration of malonaldehyde(mdA) in the lung and intestinal tissue were assessed according to the corresponding *** and eosin staining was carried out to detect the pathology of the lung and *** expression levels of interleukin(IL)-6,IL-8,and tumor necrosis factor(TNF)-α in lung and intestine tissue were detected by enzyme-linked immunosorbent assay *** expression levels of Fas and Bcl2 in lung tissues were determined by immunohistochemistry and Western ***:Septic shock elicited a significant increase in the levels of mdA(10.17 ± 1.12 nmol/mg protein vs 2.98 ± 0.64 nmol/mg protein) and MPO(6.79 ± 1.02 U/g wet tissue vs 1.69 ± 0.14 U/g wet tissue) in lung *** effects were not significantly decreased by Group C pretreatment,but were significantly reduced by Group D pretreatment(mdA:4.45 ± 1.13 nmol/mg protein vs 9.56 ± 1.37 nmol/mg protein;MPO:2.58 ± 0.21 U/g wet tissue vs 6.02 ± 1.16 U/g wet tissue).The activity of SOD(250.32 ± 8.56 U/mg protein vs 365.78 ± 10.26 U/mg protein) in lung tissues was decreased after septic shock,and was not significantly increased by Group C pretreatment,but was significantly enhanced by Group D pretreatment(331.15 ± 9.64 U/mg protein vs 262.98 ± 5.47 U/mg protein).Histological evidence of lung hemorrhage,neutrophil infiltration and overexpression of IL-6,IL-8,and TNF-α was observed in lung tissues,all of which were attenuated by Group C and fur

关键词： Early fluid resuscitation Inhalation of hydrogen gas Septic shock lung Intestine Oxidative damage

Electroacupuncture treatment improves motor function and neurological outcomes after cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2022年第7期17卷 1545-1555页

作者： Si-Si Li Xu-Yun Hua Mou-Xiong Zheng Jia-Jia Wu Zhen-Zhen ma Xiang-Xin Xing Jie ma Chun-Lei shan Jian-Guang Xu school of Rehabilitation Science Shanghai University of Traditional Chinese MedicineShanghaiChina Department of Traumatology and Orthopedics Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghaiChina Center of Rehabilitation Medicine Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghaiChina Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation Ministry of EducationShanghaiChina

Electroacupuncture(EA)has been widely used for functional restoration after ***,its role in post-stroke rehabilitation and the associated regulatory mechanisms remain poorly *** this study,we applied EA to the Zusanli(ST36)and Quchi(LI11)acupoints in rats with middle cerebral artery occlusion and *** found that EA effectively increased the expression of brain-derived neurotrophic factor and its receptor tyrosine kinase B,synapsin-1,postsynaptic dense protein 95,and microtubule-associated protein 2 in the ischemic penumbra of rats with middle cerebral artery occlusion and ***,EA greatly reduced the expression of myelin-related inhibitors Nogo-A and NgR in the ischemic *** kinase B inhibitor ANA-12 weakened the therapeutic effects of *** findings suggest that EA can improve neurological function after middle cerebral artery occlusion and reperfusion,possibly through regulating the activity of the brain-derived neurotrophic factor/tyrosine kinase B signal *** procedures and experiments were approved by the Animal Research Committee of shanghai university of Traditional Chinese Medicine,China(approval ***200110002)on January 10,2020.

关键词： brain-derived neurotrophic factor dendritic electroacupuncture ischemia/reperfusion motor function neurite outgrowth inhibitor-A neurological outcomes Nogo receptor synapse tyrosine kinase B

抗TL1A单克隆抗体通过影响巨噬细胞极化减轻结节病肉芽肿形成的机制研究

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临床肺科杂志 2024年第8期29卷 1146-1151,1156页

作者：马成星魏佳单佳佳郑颖温艳婷丁晶晶南京大学医学院附属鼓楼医院呼吸与危重症医学科江苏南京210008 南京中医药大学鼓楼临床医学院呼吸与危重症医学科江苏南京210008 南京大学医学院基础医学部江苏南京210008

目的探讨抗TL1A单克隆抗体是否可通过影响结节病中巨噬细胞的极化水平,减轻结节病肉芽肿的形成。方法采用随机分组法将32只C57BL/6小鼠分成空白对照组、结节病组、结节病+TL1A Ab治疗组和结节病+地塞米松治疗组(n=8)。通过肺组织病理... 详细信息

目的探讨抗TL1A单克隆抗体是否可通过影响结节病中巨噬细胞的极化水平,减轻结节病肉芽肿的形成。方法采用随机分组法将32只C57BL/6小鼠分成空白对照组、结节病组、结节病+TL1A Ab治疗组和结节病+地塞米松治疗组(n=8)。通过肺组织病理学染色(HE染色)观察各组小鼠肺部肉芽肿的形成;通过流式细胞术检测各组小鼠脾脏单个核细胞(PBMC)中M1/M2型巨噬细胞的水平。提取各组小鼠股骨和胫骨内的原代骨髓源性巨噬细胞(BmdM),培养并分别诱导各组BmdM向M1或M2型巨噬细胞方向分化,使用流式细胞术与qPCR分别测定各组小鼠BmdM中M1型巨噬细胞和M2型巨噬细胞的细胞数目和相关因子的mRNA表达情况。结果与结节病组相比,结节病+TL1A Ab组小鼠肺组织中肉芽肿的数目明显减少(PmdM经诱导分化后,结节病+TL1A Ab组M1型巨噬细胞极化水平较结节病组下降(P<0.001);而TL1A Ab组M2型巨噬细胞极化水平高于结节病组(P<0.001)。结论抗TL1A单克隆抗体通过调控结节病中巨噬细胞的极化水平而抑制结节病中的肉芽肿性炎。

关键词：结节病肉芽肿巨噬细胞抗TL1A单克隆抗体

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中药内服治疗高尿酸血症疗效的Meta分析

内蒙古医学杂志 2024年第5期56卷 573-579页

作者：吴媛媛刘正奇宋珊马建辉刘永听贵州中医药大学贵阳550025 贵州中医药大学第二附属医院贵阳550003

目的系统评价中药内服治疗高尿酸血症(HUA)的疗效。方法通过检索万方数据库(Wan-Fang Data)、中国知网(CNKI)、维普数据库、PubMed及Cochrane图书馆等相关数据库,搜索内服中药治疗高尿酸血症的随机对照试验(RCT),检索时间为2013年1月至2... 详细信息

目的系统评价中药内服治疗高尿酸血症(HUA)的疗效。方法通过检索万方数据库(Wan-Fang Data)、中国知网(CNKI)、维普数据库、PubMed及Cochrane图书馆等相关数据库,搜索内服中药治疗高尿酸血症的随机对照试验(RCT),检索时间为2013年1月至2023年1月,采用Revman5.4进行分析。结果共纳入符合标准的8篇文献,共642例患者,Meta分析结果显示,口服中药治疗高尿酸血症的血尿酸浓度(I_(2)=0%50%,P=0.1)和降尿酸疗效(I_(2)=0%md=-0.43,95%CI(-0.62,-0.24),Pmd=-0.47,95%CI(-0.60,-0.34),P<0.00001]差异具有统计学意义,其疗效均优于单纯西药治疗。结论口服中药联合西药治疗高尿酸血症疗效优于单纯西药治疗。

关键词：高尿酸血症中药 Meta分析

Expression and effect of sodium-potassium-chloride cotransporter on dorsal root ganglion neurons in a rat model of chronic constriction injury

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Neural Regeneration Research 2020年第5期15卷 912-921页

作者： Chao-Yang Tan Yan-Ping Wang Yuan-Yuan Han Bi-Han lu Wei Ji Li-Cang Zhu Yang Wang Wen-Yan Shi Li-Ya shan Liang Zhang Ke-Tao ma Li Li Jun-Qiang Si Department of Physiology College of MedicineShihezi UniversityShiheziXinjiang Uygur Autonomous RegionChina Department of Physiology Medical College of Jiaxing UniversityJiaxingZhejiang ProvinceChina Department of Health Karamay Army DivisionChinese People’s Liberation ArmyKaramayXinjiang Uygur Autonomous RegionChina Department of Nursing Medical College of Jiaxing UniversityJiaxingZhejiang ProvinceChina Department of Clinical Medicine Karamay College of Xinjiang Medical UniversityKaramayXinjiang Uygur Autonomous RegionChina Department of Neurosurgery First Affiliated HospitalCollege of MedicineShihezi UniversityShiheziXinjiang Uygur Autonomous RegionChina The key Laboratory of Xinjiang Endemic and Ethnic Diseases College of MedicineShihezi UniversityShiheziXinjiang Uygur Autonomous RegionChina Department of Physiology School of Basic Medical SciencesWuhan UniversityWuhanHubei ProvinceChina Department of Physiology School of Basic Medical SciencesHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Sodium-potassium-chloride cotransporter 1 (NKCC1) and potassium-chloride cotransporter 2 (KCC2) are associated with the transmission of peripheral *** investigated whether the increase of NKCC1 and KCC2 is associated with peripheral pain transmission in dorsal root ganglion *** this aim,rats with persistent hyperalgesia were randomly divided into four *** in the control group received no treatment,and the rat sciatic nerve was only exposed in the sham *** in the chronic constriction injury group were established into chronic constriction injury models by ligating sciatic nerve and rats were given bumetanide,an inhibitor of NKCC1,based on chronic constriction injury modeling in the chronic constriction injury + bumetanide *** the experiment measuring thermal withdrawal latency,bumetanide (15 mg/kg) was intravenously *** the patch clamp experiment,bumetanide (10 μg/μL) and acutely isolated dorsal root ganglion neurons (on day 14) were incubated for 1 hour,or bumetanide (5 μg/μL) was intrathecally *** Hargreaves test was conducted to detect changes in thermal hyperalgesia in *** found that the thermal withdrawal latency of rats was significantly decreased on days 7,14,and 21 after model *** intravenous injection of bumetanide,the reduction in thermal retraction latency caused by model establishment was significantly *** and western blot assay results revealed that the immune response and protein expression of NKCC1 in dorsal root ganglion neurons of the chronic constriction injury group increased significantly on days 7,14,and 21 after model *** immune response or protein expression of KCC2 was observed in dorsal root ganglion neurons before and after model *** Cl^– (chloride ion) fluorescent probe technique was used to evaluate the change of Cl^– concentration in dorsal root ganglion neurons of chronic constriction injury model *** found th

关键词： bumetanide chronic constriction injury dorsal root ganglion dorsal root reflex hyperalgesia KCC2 nerve regeneration neuropathic pain NKCC1 primary afferent depolarization whole-cell patch clamp

脊髓栓系综合征大鼠后肢骨骼肌萎缩及miRNA-206表达变化的早期观察

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中华小儿外科杂志 2024年第5期45卷 449-454页

作者：夏冰王文静刘福云程志伟胡伟明赵裕丰马凤群王恺付家乐单向阳郑州大学第三附属医院骨科郑州450052 郑州大学第三附属医院病案科郑州450052

目的通过建立脊髓栓系综合征(tethered cord syndrome,TCS)大鼠模型,观察后肢胫后肌群骨骼肌萎缩的早期病理改变,探讨miRNA-206在此过程中的变化及其对骨骼肌萎缩的影响。方法采用手术方法制备TCS大鼠模型。观察造模与拮抗剂处理后大鼠... 详细信息

目的通过建立脊髓栓系综合征(tethered cord syndrome,TCS)大鼠模型,观察后肢胫后肌群骨骼肌萎缩的早期病理改变,探讨miRNA-206在此过程中的变化及其对骨骼肌萎缩的影响。方法采用手术方法制备TCS大鼠模型。观察造模与拮抗剂处理后大鼠后肢胫后肌群骨骼肌萎缩的早期功能障碍和肌肉体积、肌湿重(比)、肌纤维平均横截面积的变化,并采用实时荧光定量聚合酶链反应检测miRNA-206在大鼠后肢胫后肌群中的表达。结果术后7 d起,模型组大鼠后肢开始出现功能障碍。28 d时模型组miRNA-206表达明显升高,是假手术组的5.54倍,肌湿重、肌纤维平均横截面积减小,与假手术28 d组比较,差异有统计学意义(均P<0.05)。给予miRNA-206拮抗剂后,肌纤维平均横截面积及胫后肌群体积明显缩小,肌湿重比减少,差异有统计学意义(P<0.05)。结论TCS模型大鼠后肢胫后肌群骨骼肌萎缩的早期病理改变会造成miRNA-206表达显著增加,这可能是机体的一种自我保护机制。

关键词：神经管缺损脊髓栓系综合征大鼠肌肉萎缩 miRNA-206