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A microarray analysis of early activated pathways in concanavalin A-induced hepatitis

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第5期11卷 366-377页

作者： Qing-yi CAO Feng CHEN Jie LI shan-shan wu Jing WANG Zhi CHEN State Key Laboratory for Diagnosis and Treatment of Infectious Diseases the First Affiliated Hospital School ofl Medicine Zhejiang University Hangzhou 310003 China

Objective:To explore the mechanisms of fulminant hepatitis(FH) in the early stages,and to determine the critical pathways in its initiation and ***:Twelve BALB/c mice were divided into four groups:one group left as negative control and sacrificed immediately after injection of phosphate-buffered saline(PBS),and another three groups with concanavalin A(Con A) administration sacrificed at 1,3,and 6 h after *** GeneChip Mouse 430 2.0 Array was employed to evaluate the expression profile of each of the 12 *** analysis was done on the microarray data to extract the genes that were differentially *** analysis was carried out to determine relevant pathways within which regulated genes were significantly ***:A total of 393,8354 and 11 344 differentially expressed genes were found,respectively,at three time *** 0-1 h and 1-3 h,most of the pathways enriched with regulated genes were related to immune response and inflammation,among which Toll-like receptor(TLR) signaling and mitogen-activated protein kinase(MAPK) signaling appeared during both phases,while cytokine-cytokine receptor interaction,apoptosis,T cell receptor signaling,and natural killer(NK) cell-mediated cytotoxicity pathways emerged during the second *** found to be significant during 3-6 h were mostly related to metabolic ***:The TLR signaling pathway dominates the early responses of Con A-induced FH in *** stimulates the production of type I cytokines,therefore recruiting and activating T/NK *** T/NK cells exert their cytotoxicity on hepatocytes through inducing death receptorintermediated apoptosis,resulting in liver injury.

关键词： Concanavalin A Fulminant hepatitis Microarray Expression profile Pathway analysis

人参皂苷Rb1通过SDF-1/CXCR4通路调节牙周膜干细胞增殖及成骨分化

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中国新药与临床杂志 2024年第8期43卷 594-599页

作者：吴珊赵国廷董振耀马秀花姚毅章青海省第五人民医院口腔科青海西宁810007

目的观察人参皂苷Rb1(Gs Rb1)对人牙周膜干细胞(h PDLSCs)增殖和成骨分化的影响,及其与基质细胞衍生因子1(SDF-1)/CXC趋化因子受体4(CXCR4)通路相关分子机制。方法采用酶消化法分离、培养h PDLSCs,倒置显微镜下观察细胞形态,流式细胞术... 详细信息

目的观察人参皂苷Rb1(Gs Rb1)对人牙周膜干细胞(h PDLSCs)增殖和成骨分化的影响,及其与基质细胞衍生因子1(SDF-1)/CXC趋化因子受体4(CXCR4)通路相关分子机制。方法采用酶消化法分离、培养h PDLSCs,倒置显微镜下观察细胞形态,流式细胞术检测基质细胞抗原1(STRO-1)、分化簇146(CD146)阳性细胞比例;分别用0、0.5、1.0、2.0、4.0、6.0μmol·L^(-1)GsRb1处理h PDLSCs,CCK-8法筛选最佳作用浓度。将h PDLSCs分为对照组、Gs Rb1(4.0μmol·L^(-1))组、AMD3100(5μg·m L^(-1))组、Gs Rb1+AMD3100组,比较各组细胞增殖活力和碱性磷酸酶(ALP)活性,RT-q PCR检测Runt相关基因2(Runx2)、oxterix、骨桥蛋白(OPN)m RNA表达情况,茜素红染色、定量分析检测h PDLSCs中矿化结节形成量,Western blot法检测SDF-1/CXCR4信号通路相关蛋白表达情况。结果h PDLSCs呈放射状排列,形态为长梭形,生长较为致密,STRO-1、CD146阳性细胞比例分别为97.19%、98.01%。随着Gs Rb1浓度的增加,h PDLSCs增殖活力增强,呈浓度依赖性(P<0.05)。与对照组比较,Gs Rb1组细胞增殖活力、ALP活性增强,runx2、oxterix、OPN mRNA表达增加,矿化结节形成量及SDF-1、CXCR4蛋白表达均增加(P<0.05),而AMD3100组与之相反(P<0.05)。与Gs Rb1组比较,Gs Rb1+AMD3100组细胞增殖活力、ALP活性降低,Runx2、oxterix、OPN m RNA表达降低,矿化结节形成量及SDF-1、CXCR4蛋白表达均减少(P<0.05)。结论Gs Rb1能够促进h PDLSCs增殖及成骨分化,可能与增加SDF-1/CXCR4信号通路蛋白表达有关。

关键词：人参皂苷Rb1 基质细胞衍生因子1 CXC趋化因子受体4 牙周组织间质干细胞移植细胞增殖

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酸羊乳发酵及其健康功效研究进展

中国乳品工业 2024年第2期52卷 38-45页

作者：张文静毛咏荷李宁赵月娟吴珊刘军中国农业大学食品科学与营养工程学院北京100091 西安银桥乳业(集团)有限公司西安710075

羊乳中蛋白质、脂肪、维生素及矿物质含量与牛乳相比更接近人乳,且更易消化吸收,而羊乳的膻味限制了其消费。研究指出,生物发酵方法可改善羊乳的膻味特征,提高感官品质。此外,微生物发酵过程中代谢产生短链脂肪酸、胞外多糖等功能性活... 详细信息

羊乳中蛋白质、脂肪、维生素及矿物质含量与牛乳相比更接近人乳,且更易消化吸收,而羊乳的膻味限制了其消费。研究指出,生物发酵方法可改善羊乳的膻味特征,提高感官品质。此外,微生物发酵过程中代谢产生短链脂肪酸、胞外多糖等功能性活性物质,并且伴随着羊乳中蛋白质降解,发酵产品中产生富含具有抗氧化作用、抗菌活性、ACE抑制活性等多种具有健康功效的活性肽,强化了发酵羊乳的价值。因此,采用微生物发酵方法生产营养丰富、口感独特的发酵酸羊乳是羊乳加工发展的重要方向。本文对羊乳发酵菌株及发酵工艺进行了总结分析,阐述了其对发酵羊乳口感、风味及功能活性的影响,并进一步综述了风味羊乳发酵制品创制的国内外研究现状及发展趋势,为健康美味的酸羊乳产品研发及推广提供了指导。

关键词：羊乳发酵工艺健康功效感官品质

Higher-Order Rytov Approximation for Large-Scale and Strong Perturbation Media

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Communications in Computational Physics 2020年第6期28卷 98-110页

作者： Bo Feng Ru-shan wu Huazhong Wang Wave phenomena and inversion imaging group(WPI) School of Ocean and Earth ScienceTongji UniversityShanghai200092China Modeling and Imaging Laboratory University of CaliforniaSanta Cruz95060CAUSA

In the field of geophysics,although the first-order Rytov approximation is widely used,the higher-order approximation is seldom *** both theo-retical analysis and numerical tests,the accumulated phase error introduced in the first-order Rytov approximation cannot be neglected in the presence of strong velocity *** this paper,we are focused on improving the phase accuracy of forward scattered wavefield,especially for the large-scale and strong velocity pertur-bation *** develop an equivalent source method which can update the imaginary part of the complex phase iteratively,and the higher-order scattered wavefield can be approximated by multiplying the incident wavefield by the exponent of the imaginary part of the complex *** the convergence of the proposed method has not been proved mathematically,numerical examples demonstrate that our method can produce an improved accuracy for traveltime(phase)prediction,even for strong perturbation ***,due to the neglect of the real part of the complex phase,the amplitude change of the scattered wavefield cannot be ***,in the presence of multi-arrivals phenomenon,the equivalent scattering source should be handled carefully due to the multi-directions of the *** investigations should be done to improve the applicability of the proposed method.

关键词： Rytov approximation higher-order approximation large-scale strong perturbation forward scattering equivalent scattering source

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丝瓜MYB基因家族的鉴定与表达模式分析

中草药 2024年第12期55卷 4159-4170页

作者：吴珊余小丽宋航孙淼曾厚圆杨丽欣刘雷练华山刘鹏无成都农业科技职业学院风景园林学院四川成都611130 绵阳师范学院生命科学与技术学院四川绵阳621000 成都农业科技职业学院园艺研究所四川成都611130

目的研究药食同源植物丝瓜Luffa cylindrica MYB(LcMYB)基因转录因子家族成员的结构和功能,鉴定参与抗褐化作用的主要成员及其作用机制。方法基于Pfam数据库及NCBI保守结构域分析,对丝瓜全基因组水平MYB转录因子进行鉴定。通过Expasy、M... 详细信息

目的研究药食同源植物丝瓜Luffa cylindrica MYB(LcMYB)基因转录因子家族成员的结构和功能,鉴定参与抗褐化作用的主要成员及其作用机制。方法基于Pfam数据库及NCBI保守结构域分析,对丝瓜全基因组水平MYB转录因子进行鉴定。通过Expasy、MEME等在线工具分析LcMYB转录因子家族蛋白理化性质、顺式作用元件及保守结构域。然后利用MEGA软件构建系统发育树,分析丝瓜与拟南芥、苹果、番茄、茄子及马铃薯等植物MYB蛋白的系统发育关系。结合2个褐化敏感程度不同的丝瓜转录组,筛选了丝瓜褐化相关的关键MYB转录因子及其靶基因。结果全基因组水平共鉴定到449个具有相似基因结构及保守基序的MYB基因,可聚类为4个亚家族(R1_MYB、R2R3_MYB、R3_MYB和R4_MYB),并在正反链均存在可能的顺式调控元件。靶基因及基因表达分析结果显示多个MYB及其靶基因尤其是多铜氧化酶相关基因可能在鲜切丝瓜的不同褐化阶段起重要的作用,是丝瓜褐化的关键调节因子。结论系统鉴定了LcMYB家族基因,并鉴定了可能参与丝瓜褐化的MYB基因及其靶基因,有助于进一步了解和丰富LcMYB基因家族的生理功能,并为进一步解析果实褐化调控机制,提高果实品质提供了候选基因。

关键词：丝瓜 MYB基因家族转录因子褐化多铜氧化酶

Variant selection of nanocrystalline a phase in metastableβTB8 titanium alloy via electric pulse treatment

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稀有金属:英文版 2023年第8期42卷 2781-2794页

作者： shan-shan wu Ya Yang Yuan-Biao Tan Song Xiang Ya-Li Zhou Min Ma Fei Zhao Wei Shi Guizhou Key Laboratory of Materials Mechanical Behavior and Microstructure College of Materials and MetallurgyGuizhou UniversityGuiyang 550025China College of Materials Science and Engineering Hunan University of Science and TechnologyXiangtan 411201China

In this paper,the metastableβTB8 titanium alloy with nanocrystallineαphase is achieved by electric pulse *** morphology evolution and variant selection of nanocrystallineαphase in metastableβTB8titanium alloy were investigated by using scanning electron microscope(SEM),electron backscattered diffraction(EBSD)and transmission electron microscope(TEM)*** results indicated that the morphologies of the nanocrystallineαphase were mainly triangular clusters and needle-like at the pressure of 0 *** increasing pressure from 20 to 50 MPa,the volume fraction of needlelikeαphase decreased,and a large amount of V-shapedαphase formed in the interior ofβ*** on the EBSD data,the parentβphase was reconstructed by MTEX *** the interior of theβgrains,12 variants can form for the samples electric pulse treated at 0 and20 MPa,while only 3 and 6 variants can form for the samples electric pulse treated at 30 and 50 *** the grain boundary of theβgrains,one or more grain boundaryαvariants can be generated for the samples electric pulse treated at different pressures as long as one of the neighborβgrains follows the Burgers orientation relationship.

关键词： TB8 titanium alloy Parentβgrain reconstruction Morphology ofαphase Variant selection

Experimental study on the penetration characteristics of leaking molten salt in the thermal insulation layer of aluminum silicate fiber

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Nuclear Science and Techniques 2021年第9期32卷 27-39页

作者： Yun Wang Jian Tian shan-wu Wang Chong Zhou Na-Xiu Wang shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China University of Chinese Academy of Sciences Beijing 100049China

The molten salt leakage accident is an important issue in the nuclear safety analysis of molten salt *** the molten salt leaks from the pipeline or storage tank,it will contact the insulation layer outside;hence,the processes of penetration and spreading play an important role in the development of leakage *** this study,the penetration and diffusion of leaking molten salt(LMS)in an aluminum silicate fiber(ASF)thermal insulation layer were studied experimentally.A molten salt tank with an adjustable outlet was designed to simulate the leakage of molten salt,and the subsequent behavior in the thermal insulation layer was evaluated by measuring the penetra-tion time and penetration mass of the *** results show that when the molten salt discharges from the outlet and reaches the thermal insulation layer,the LMS will penetrate and seep out from the ASF,and a higher flow rate of LMS requires less penetration time and leaked mass of *** the temperature of the LMS and thickness of the ASF increased,the penetration time became longer and the leaked mass became greater at a lower LMS flow rate;when the LMS flow rate increased,the penetration time and leaked mass decreased rapidly and tended to flatten.

关键词： Molten salt reactor Molten salt leakage Penetration Insulation layer

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纵切自动车床环境温度变化误差试验研究

环境技术 2024年第2期42卷 127-131页

作者：吴珊孔令飞习璐张沛咸阳职业技术学院汽车工程学院咸阳712000 西安理工大学机械与精密仪器工程学院西安710048

为研究环境温度变化对纵切自动车床热误差的影响,本文以RTS20瑞士型纵切自动车床为试验载体,通过环境温度变化误差(ETVE)试验装置,开展了恒主轴转速(5000 rpm)变环境温度、变主轴转速(0~8000 rpm)变环境温度的热误差试验,对环境温度对... 详细信息

为研究环境温度变化对纵切自动车床热误差的影响,本文以RTS20瑞士型纵切自动车床为试验载体,通过环境温度变化误差(ETVE)试验装置,开展了恒主轴转速(5000 rpm)变环境温度、变主轴转速(0~8000 rpm)变环境温度的热误差试验,对环境温度对热误差的影响进行了研究分析。结果表明:在相同主轴转速下,环境温度变化影响着车床结构各部位的温升,进而影响热误差;在随机主轴转速下,主轴轴承等温度测点的温升受转速变化的影响,热误差的增长与关键测点温度具有相同变化周期,但不具有相同变化趋势,即温度快速升高时热误差并不一定也正向增长;空调开启后各测点温度有明显降低,最高降幅可达3℃,热误差随之也有明显降低趋势,最高降幅达6μm。

关键词：环境温度温度变化试验纵切车床热误差

探索基于增强CT影像组学鉴别良恶性腮腺肿瘤的应用价值

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中国CT和MRI杂志 2024年第6期22卷 50-52页

作者：杜峻吴山刘志荣山西白求恩医院(山西医学科学院同济山西医院) 山西医科大学第三医院口腔科山西太原030032 山西白求恩医院(山西医学科学院同济山西医院) 山西医科大学第三医院影像科山西太原030032

目的利用增强CT影像组学特征构建影像组学模型并绘制列线图鉴别良恶性腮腺肿瘤。方法回顾性分析山西白求恩医院行颌面部两期增强CT患者103例,所有患者经病理证实均为腮腺肿瘤(良性71例,恶性32例)。于两期增强CT图像手动勾画肿瘤ROI,... 详细信息

目的利用增强CT影像组学特征构建影像组学模型并绘制列线图鉴别良恶性腮腺肿瘤。方法回顾性分析山西白求恩医院行颌面部两期增强CT患者103例,所有患者经病理证实均为腮腺肿瘤(良性71例,恶性32例)。于两期增强CT图像手动勾画肿瘤ROI,提取组学特征采用LASSO降维筛选特征后计算出影像组学评分(rad-score)并建立影像组学预测模型并绘制列线图。采用ROC曲线对模型性能评估,通过实施Hosmer-Lemeshow适合度评估模型的统计拟合度并绘制了校正曲线(calibration curve)。采用决策曲线分析(decision curve analysis,DCA)进一步探讨模型的临床应用价值。结果根据rad-score建立的LR模型在训练集和测试集的AUC分别为0.848(95%CI 0.24-0.931)和0.826(95%CI 0.711-0.925)。校准曲线显示出良好的一致性。决策曲线分析显示了显著净收益的增加。结论基于增强CT影像组学在鉴别良恶性腮腺肿瘤具有术前诊断价值,有助于临床精准诊疗。

关键词：腮腺肿瘤计算机断层扫描影像组学

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