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Antibacterial efficacy of five medicinal plants against multidrug-resistant enteropathogenic bacteria infecting under-5 hospitalized children

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Journal of Integrative Medicine 2015年第1期13卷 45-57页

作者： shakti rath Rabindra N.Padhy Department of Botany and Biotechnology B.J.B. Autonomous College Central Research Laboratory IMS & Sum Hospital Medical College Siksha ‘O' Anusandhan University

OBJECTIVE： To evaluate in vitro antibacterial effectiveness of five medicinal plants used by an Indian aborigine, against 8 multidrug-resistant （MDR） enteropathogenic bacteria isolated from clinical samples of under-5 hospitalized children. METHODS： Antibiotic sensitivity patterns of eight clinically isolated strains of enteropathogenic bacteria, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Salmonella paratyphi, S. typhi, Shigella dysenteriae, S. sonnei and Vibrio cholerae were assessed by disc-diffusion method. Antibacterial activities of 8 solvent-extracts of leaves and bark of five medicinal plants were monitored by the agar-well diffusion method. The microbroth dilution method was used to assess minimum inhibitory concentration （MIC） and minimum bactericidal concentration （MBC）. Qualitative phytochemical analyses of active plant extracts were carried out. RESULTS： Ethanol, ethyl acetate and methanol extracts of Holarrhena antidysenterica leaf tissue were most effective against 8 MDR pathogens in vitro. Similarly, acetone, ethanol and methanol extracts of Terminalia alata leaf tissue; chloroform, ethyl acetate and methanol extracts of Terminalia aquna leaf tissue and ethyl acetate, ethanol and methanol extracts of Paederia foetida leaf tissue were most effective in inhibiting in vitro growth of the 8 MDR enteropathogens. Ethyl acetate and methanol extracts of H. antidysenterica bark tissue; acetone, ethanol and methanol extracts of T. alata bark tissue and acetone, ethanol and methanol extracts of T. arjuna bark tissue were most effective in controlling enteropathogen growth. The minimum inhibitory concentration and minimum bactericidal concentration values of the 3 most antimicrobial leaf and bark extracts from the five plants were in the range of 1.56 to 50 mg/mL. CONCLUSION： These 5 plants exhibited in vitro control over a cohort of 8 enteropathogenic bacteria strains isolated from clinical samples.

关键词： ethnomedicinal plants multidrug resistance enteropathogenic bacteria antibacterial assay phytochemical analysis plants, medicinal

Probit Analysis of Carbamate-Pesticide-Toxicity at Soil-Water Interface to N_2-Fixing Cyanobacterium Cylindrospermum sp.

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Rice science 2015年第2期22卷 89-98页

作者： Rabindra N.PADHY shakti rath Department of Botany BJB Autonomous College

Toxicity-data of two carbamate insecticides, carbaryl and carbofuran, and three fungicides, ziram, zineb and mancozeb with rice-field N2-fixing cyanobacterium Cylindrospermum sp., obtained by in vitro growth and at soil-water interface, were analyzed by the probit method. Growth enhancing concentration, no-observed effective concentration, minimum inhibitory concentration, the highest permissive concentration and lethal concentration100 （LCloo） were determined experimentally. The LC^o values of carbaryl, carbofuran, ziram, zineb and mancozeb in N2-fixing liquid medium were 56.2, 588.8, 0.07, 4.2 and 3.4 IJg/mL, respectively, whereas the corresponding LCloo values were 100.0, 1500.0, 0.17, 25.0 and 9.0 IJg/mL, respectively. The LC50 values of these pesticides in succession in N2-fixing agar medium were 44.7, 239.9, 0.07, 1.8 and 2.3 IJg/mL, respectively, whereas the corresponding LC100 values were 100.0, 600.0, 0.17, 10.0 and 7.0 IJg/mL, respectively. Similar results with nitrate supplemented liquid and agar media indicated that nitrate supplementation had toxicity reducing effect. The LCso and LC^oo values of toxicity in the N2-fixing liquid medium at soil-water interface were 91.2 and 200.0 IJg/mL for carbaryl, 2 317 and 6 000 pg/mL for carbofuran, 0.15 and 0.50 pg/mL for ziram, 16.4 and 50.0 pg/mL for zineb, and 7.2 and 25.0 pg/mL for mancozeb, respectively. Each LC^oo value at soil- water interface with a pesticide was significantly higher than its corresponding LCloo value at liquid/agar media. It can be concluded that, under the N2-fixing conditions, the cyanobacterium tolerated higher levels of each pesticide at soil-water interface.

关键词： carbamate pesticide Cylindrospermum sp. cyanobacterium lethal concentration probitanalysis soil-water interface

Natural therapeutics for cancer treatment:success,challenges,and prospect

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Journal of Bio-X Research 2023年第2期6卷 61-72页

作者： Debasmita Dubey shakti rath Rajesh Kumar Meher Sanghamitra Mishra Soumya Surath Panda Subhashree Ray Subrat Kumar Tripathy Medical Research Laboratory IMS and SUM HospitalSiksha“O”Anusandhan Deemed to be UniversityK8Kalinga NagarBhubaneswar 751003OdishaIndia Central Research Laboratory Institute of Dental SciencesSiksha“O”Anusandhan Deemed to be UniversityK8Kalinga NagarBhubaneswar 751003OdishaIndia Centre of Excellence in Natural Products and Therapeutics Department of Biotechnology and BioinformaticsSambalpur UniversityJyotiviharBurlaSambalpurOdishaIndia Department of Critical Care Medicine IMS and SUM HospitalSiksha“O”Anusandhan Deemed to be UniversityK8Kalinga NagarBhubaneswar 751003OdishaIndia Department of Oncology IMS and SUM HospitalSiksha“O”Anusandhan Deemed to be UniversityK8Kalinga NagarBhubaneswar 751003OdishaIndia Department of Biochemistry IMS and SUM HospitalSiksha“O”Anusandhan Deemed to be UniversityK8Kalinga NagarBhubaneswar-751003OdishaIndia.

Cancer is a terrifying disease that has become one of the world’s most challenging health concerns,necessitating a proactive approach to its treatment.Nature is a rich source of new chemical entities and a promising avenue for cancer research.Because of their different modes of action on target events in many ways,nature-derived chemicals are considered good possibilities for anticancer drug development.The current review article highlights recent breakthroughs in cancer therapeutics,including natural therapeutic molecules and their mechanisms of action against cancer treatment,as well as their limits,challenges,and prospect.For the review,article data have been collected from previously studied and published research reports,clinical evaluations,case studies,and clinical statistical data generated by scientific research organizations of repute.The chemical structures have been drawn using computational software(chemsketch).The development of promising candidates that can prevent or reduce the proliferation of cancer cells without causing adverse effects from these therapeutics is now underway.Further,nature-derived products are a game-changer because they are simple,safer,environmentally friendly,low-cost,quick,and less toxic than traditional treatment techniques.However,many bioactive chemicals and their analogs have been discovered as possible anticancer treatment options.In the review,the potent therapeutics phytochemicals derived from nature with their tremendous anticancer effects with limitations and prospects have been studied and analyzed based on previously published articles and reports.

关键词： cancer treatment chemotherapy drug resistance natural therapy toxicity

Comparative oral metagenome insight into acute and chronic root canal infections

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Journal of Bio-X Research 2023年第4期6卷 117-123页

作者： Kumari K.Swapna Sangita Dixit Mahendra Gaur Dibyajyoti Uttameswar Behera Patitapabana Das Enketeswara Subudhi shakti rath Institute of Dental Sciences Siksha"O"Anusandhan(Deemed to be University)Bhubaneswar 751003OdishaIndia Centre for Biotechnology School of Pharmaceutical SciencesSiksha"O"Anusandhan(Deemed to be University)Bhubaneswar 751003OdishaIndia

Background:In the dynamic progression of root canal infections,the resultant change in environmental conditions directs the adaptation of the microbiome in terms of richness,evenness,and diversity.Objective:The goal of this study is to contrast the interradicular bacterial community structures of teeth in acute and chronic stages and investigate potential microorganisms associated with persistent apical periapical infections.Methods:Interradicular samples were collected from 10 teeth in the apical periodontitis group and 10 teeth in the chronic apical periodontitis group.High-throughput next-generation sequencing(NGS)–based microbial identification through the Illumina platform was used to characterize the complex intraradicular microbial communities in acute and chronic root canal infections.Results:Bacteria were the major domain,with a prevalence of>97%(acute cases:98.11%and chronic cases:97.6%)compared to eukaryotes and archaea.Bacterial diversity was relatively higher in acute compared to chronic conditions under 10 and 9 phyla,79 and 64 genera,and 172 and 135 species,respectively.These endodontically infecting bacteria belonged to Firmicutes(47.21%),Bacteroidetes(20.07%),and Proteobacteria(10.72%)in acute conditions,while in the chronic stage,Firmicutes(40.85%),Proteobacteria(27.80%),and Actinobacteria(14.79%)were abundant.The phylum Candidatus Saccharibacteria was exclusively found in acute samples.Spirochaetes and Synergistetes were found predominantly in acute infections compared to chronic infections.Bacillus and Lactobacillus were found in equal and highest abundance under the phylum Firmicutes in both conditions.Conclusion:This study provides information on the differential microbial community present in the apical root canal system of teeth with acute and chronic apical periodontitis and contributes to new approaches or channels for future research on preventive measures and therapeutic protocols during disease progression and treatment.However,functional characterization and detection of antimicrobia

关键词： acute chronic apical periodontitis infections metagenome oral microbiome

Growth, Metabolism and Yield of Rice Cultivated in Soils Amended with Fly Ash and Cyanobacteria and Metal Loads in Plant Parts

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Rice science 2016年第1期23卷 22-32页

作者： Rabindra N.PADHY Nabakishore NAYAK Rajesh R.DASH-MOHINI shakti rath Rajani K.SAHU Department of Botany and Biotechnology Buxi Jagabandhu Bidyadhar Autonomous College Central Research Laboratory Institute of Medical Sciences & Sum HospitalSiksha ‘O' Anusandhan University

Soil amendment with fly ash（FA） and combined supplementation with N_2-fixing cyanobacteria masses as biofertilizer were done in field experiments with rice. Amendments with FA levels, 0, 0.5, 1.0, 2.0, 4.0, 8.0 and 10.0 kg/m2, caused increase in growth and yield of rice up to 8.0 kg/m2, monitored with several parameters. Pigment contents and enzyme activities of leaves were enhanced by FA, with the maximum level of FA at 10.0 kg/m2. Protein content of rice seeds was the highest in plants grown at FA level 4.0 kg/m2. Basic soil properties, p H value, percentage of silt, percentage of clay, water-holding capacity, electrical conductivity, cation exchange capacity, and organic carbon content increased due to the FA amendment. Parallel supplementation of FA amended plots with 1.0 kg/m2 N_2-fixing cyanobacteria mass caused further significant increments of the most soil properties, and rice growth and yield parameters. 1000-grain weight of rice plants grown at FA level 4.0 kg/m2 along with cyanobacteria supplementation was the maximum. Cyanobacteria supplementation caused increase of important basic properties of soil including the total N-content. Estimations of elemental content in soils and plant parts（root and seed） were done by the atomic absorption spectrophotometry. Accumulations of K, P, Fe and several plant micronutrients（Mn, Ni, Co, Zn and Cu） and toxic elements（Pb, Cr and Cd） increased in soils and plant parts as a function of the FA gradation, but Na content remained almost unchanged in soils and seeds. Supplementation of cyanobacteria had ameliorating effect on toxic metal contents of soils and plant parts. The FA level 4.0 kg/m2, with 1.0 kg/m2 cyanobacteria mass supplementation, could be taken ideal, since there would be recharging of the soil with essential micronutrients as well as toxic chemicals in comparative lesser proportions, and cyanobacteria mass would cause lessening toxic metal loads with usual N_2-fixation.

关键词： soil property cyanobacteria fly ash growth heavy metal rice toxic element yield

A report on infection dynamics of inducible clindamycin resistance of Staphylococcus aureus isolated from a teaching hospital in India

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Asian Pacific Journal of Tropical Biomedicine 2013年第2期3卷 148-153页

作者： Debasmita Dubey shakti rath Mahesh C.Sahu Subhrajita Rout Nagen K.Debata Rabindra N.Padhy Microbiology Department IMS & Sum HospitalSiksha 'O' Anusandhan University Central Research Laboratory IMS & Sum HospitalSiksha 'O' Anusandhan University

Objective:To investigate the infection of hospital-and community-acquired"erythromycininduced clindamycin resistant"strains or D-test positives of clinical isolates of Staphylococcus aureus(S.aureus)(with and without methicillin resistance)in a hospital.Methods:Strains of S.aureus isolated from clinical specimens were subjected to D-test and antibiotic profiling.Results:Of the total 278 isolates,140(50.35%)were D-test positives and the rest were D-test negatives.Further,of 140(100%)pesitives,87(62.14%)and 53(37.85%)strains were from males and females,respectively.Of 140(100%)pesitives,117(83.57%)were methicillin resistant S.aureus and23(16.42%)were methicillin sensitive S.aureus;of 140 strains,103(73.57%)strains front persons with and 37(26.42%)were without related infections;of 140 strains,91(65%)and 49(35%)were from hospital-and community-acquired samples,respectively.In 140 strains,118(84.28%)with comorbidities and 22(15.71%)without comorbidities cases were recorded;similarly,persons with prior antibiotic uses contributed 108(77.14%)and without 32(22.85%)positive strains.These binary data of surveillance were analyzed by a univariate analysis.It was evident that the prior antibiotic uses and comorbidities due to other ailments were the determinative factors in D-test positivity,corroborated by low P values,P=0.001 1 and 0.0024,respectively.All isolates(278)were resistant to17 antibiotics of nine groups,in varying degrees;the minimum of 28%resistance for vancomycin and the maximum of 97%resistance for gentamicin were recorded.Further,of 278 strains,only42(15.1%)strains were resistant constitutively to both antibiotics,erythromycin resistant and clindamycin resistant,while 45(16.2%)strains were constitutively sensitive to both antibiotics(erythromycin sensitive,and clindamycin sensitive).Further,of the rest 191(68.7%)strains were with erythromycin resistant and clindamycin resistant,of which only 140(50.35%)strains were D-test positives,while the rest 51(18.34%)strains were D-test negatives.Conclusions:In view o

关键词： Antibiotics Community-acquired D-test Erythromycin resistance Hospital-acquired Inducible clindamycin resistance MRSA MSSA Staphylococcus aureus

Early Paleozoic basement diorite of arc-magmatism from Kutch basin, Western India

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Acta Geochimica 2024年第2期43卷 296-307页

作者： Piyush Gupta Sandeep Singh shakti Singh rathore Argha Narayan Sarkar Geochronology Laboratory KDMIPEOil and Natural Gas Corporation Ltd.9Kaulagarh RoadDehradun 248195India Department of Earth Sciences Indian Institute of TechnologyRoorkee 247667India

In this study,we report for theﬁrst time an Early Palaeozoic basement diorite from the drilled well Nirona-A in the Banni Half-Graben of the Kutch basin,western India.The 40Ar–39Ar dates provided a plateau age of 441.84±2.66 Ma and another pseudo plateau of 441.28±5.82 to 388.08±16.65 Ma for the basement diorite.These ages constrain the basement formation age to the Late Ordovician-Early Silurian period.The obtained basement ages are correlatable with the later part of Cambro-Ordovician alkaline magmatism that has been reported from the Huqf area in Central Oman,whereas their lithological and petrographic correlativity with base-ment diorites occurring in the Dinsi Body of Nagar Parkar igneous complex in Pakistan can also be envisaged.The geochemical studies characterized the diorite with enrich-ment of LILE(Rb,Ba,and K)and LREE(La,Ce,Nd),strong depletion of HFSE(Nb,Sr,P,and Ti),along with weakly negative Eu anomalies.The geochemical signatures indicate their petrogenetic afﬁliation with mantle-derived magmas,as well as their tectonic setting to be arc-related,having post-collisional continental-arc type afﬁnity.The*440 Ma basement of Kutch,therefore,appears to rep-resent the later thermal event associated with the reworked Neoproterozoic subduction-related suite from Greater India’s northwest edge,which has implications for Gond-wana assembly in the northwest Indian subcontinent.

关键词： Kutch basin 40Ar–39Ar ages Early palaeozoic basement Continental-arc magmatism Calc-alkaline magmatism

Performance and combustion study of a low heat rejection engine running with biogas–diethyl ether–diesel

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Waste Disposal and Sustainable Energy 2024年第1期6卷 151-162页

作者： Sanjaya K.Mishra Premananda Pradhan shakti P.Jena Department of Mechanical Engineering S‘O’A Deemed to be UniversityBhubaneswar751030India

The present research focuses on addressing the faster depletion of fossil fuels and environmental pollution in addition to the energy crisis that hinders the progress of a nation.In the current research,waste banana leaves were considered as substrates for biogas production.Biogas is taken as the primary fuel in dual fuel(DF)operations to maximize possible diesel savings.The performance and combustion assessment were executed in a low heat rejection(LHR)engine using 5%diethyl ether by volume blended with diesel(5DEE)as pilot fuel.The combustion attributes of the engine reveal that the apex of net heat release rate(NHRR)curve retarded a bit as compared to apex of base result.The peak cylinder pressure was noted to be 6.19 MPa in the LHR engine running with 5DEE+biogas at 11.7°crank angle(CA)after top dead center(aTDC)compared to 5.23 MPa for the diesel alone operation at the same position.The apex point for NHRR was observed to be 54.51 J(°)^(−1)for 5DEE+biogas in LHR engine positioned at 3.2°aTDC.The brake thermal efficiency at full engine load operation decreased by 12.7%and 5.2%for biogas substitutions of 0.8 kg h^(−1)with diesel and 5DEE,respectively,compared to the base result.The smoke opacity and nitric oxide emissions were reduced during the DF run accompanied by diethyl ether as a fuel additive.

关键词： Biogas Banana leaves Coating Diesel engine Diethyl ether Exhaust emissions

Targeting KRAS in pancreatic cancer

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Oncology Research 2024年第5期32卷 799-805页

作者： SANDRA STICKLER BARBARA rath GERHARD HAMILTON Institute of Pharmacology Medical University of ViennaViennaA-1090Austria

Pancreatic cancer has a dismal prognosis due to late detection and lack of efficient therapies.The Kirsten rat sarcoma virus(KRAS)oncogene is mutated in up to 90%of all pancreatic ductal adenocarcinomas(PDACs)and constitutes an attractive target for therapy.However,the most common KRAS mutations in PDAC are G12D(44%),G12V(34%)and G12R(20%)that are not amenable to treatment by KRAS G12C-directed cysteine-reactive KRAS inhibitors such as Sotorasib and Adagrasib that exhibit clinical efficacy in lung cancer.KRAS G12C mutant pancreatic cancer has been treated with Sotorasib but this mutation is detected only in 2%–3%of PDAC.Recently,the KRAS G12D-directed MRTX1133 inhibitor has entered clinical trials and more of such inhibitors are in development.The other KRAS mutations may be targeted indirectly via inhibition of the cognate guanosine exchange factor(GEF)Son of Sevenless 1 that drives KRAS.These agents seem to provide the means to target the most frequent KRAS mutations in PDAC and to improve patient outcomes.

关键词： Pancreatic cancer PDAC KRAS SOS1 PROTAC