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Selection of Cocoa Tree (<i>Theobroma cacao</i>Linn) Endophytic Bacteria Solubilizing Tri-Calcium Phosphate, Isolated from Seedlings Grown on Soils of Six Producing Regions of Côte d’Ivoire

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Advances in Microbiology 2019年第9期9卷 842-852页

作者： Adama Ouattara Klotioloma Coulibaly ibrahim Konate Boubacar Ismael Kebe Abiba Sanogo Tidou Abdelkarim Filali-Maltouf UFR Agro-Forestry and Environment Department of Biochemistry and MicrobiologyLaboratory of AgrovalorizationJean Lorougnon Guede UniversityDaloaCote d’Ivoire National Center for Agronomic Research(CNRA) Laboratory of PhytopathologyDivoCote d’Ivoire Faculty of Sciences Laboratory of Microbiology and Molecular BiologyUniversity Mohammed V-AgdalRabatMorocco

Phosphorus (P) is an essential macronutrient for the growth and development of cocoa tree (Theobroma cacao Linn). Most of the soils used for cocoa farming in C?te d’Ivoire are low in phosphorus. But cocoa farmers generally have a widespread reluctance to invest in chemicals fertilizers due to high costs and environmental associated risk. Phosphate Solubilizing Bacteria (PSB) are kwon to play an important role in supply of phosphorous to plants in a sustainable manner in P deficient soils. The aim of this research was to screen the endophytic bacteria of cocoa nurseries able to solubilize tri-calcium phosphate. Seedlings of two varieties of cocoa (P7 and NA32) and seedlings of an all-comer, were grown on eighteen (18) samples soils collected in six producing regions of C?te d’Ivoire. A total of 218 endophytic bacteria were isolated and tested on the Pikovskaya’s agar medium, containing Ca3 (PO4) 2. The colonies with clear zone around the microbial growth were suspected as phosphate solubilizing bacteria. Out of 218 bacteria, 90 (41.28%) showed a clear zone around colonies after 7 days of incubation. The Phosphate Solubilization Index (PSI) ranged from 20% to 200%. Bacterial isolated from the soils of Duékoué locality showed the highest mean index of 137.67%. Five PSB (CEBSP5, CEBSP6, CEBSP7, CEBSP8, and CEBSP9) from Duékoué soils and two PSB (CEBSP12 and CEBSP13) from Soubré soils have a PSI ranged from 150% to 200%. Further study in greenhouse and in field condition will confirm the use of these PSB as biofertilizer to increase the available P content in soils, reduce environmental pollution and promote sustainable agriculture.

关键词： Endophytic Bacteria Theobroma cacao Ti-Calcium Phosphate Solubilization Biofertilizer

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SDS-聚丙烯酰胺凝胶圆盘电泳法分离乳酸脱氢酶

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新疆大学学报（自然科学版） 2000年第1期17卷 65-68页

作者：木合马德.伊布拉音邹公伟新疆大学化学系乌鲁木齐830046 南京大学化学化工学院南京210093

用连续的和不连续的SDS聚丙烯胺凝胶圆盘电泳法(SDSPAGE)对乳酸脱氢酶进行了电泳分离。

关键词： SDS-PAGE 乳酸脱氢酶分离电泳法聚丙烯酰胺

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Response of Indigenous Rhizobia to the Inoculation of Soybean[Glycine max(L.)Merrill]Varieties Cultivated under Controlled Conditions in Cote d’Ivoire

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Advances in Microbiology 2020年第3期10卷 110-122页

作者： Kouadio Amani Lassina Fondio Konate ibrahim Mako Francois DPN’Gbesso Beugre G.A.Maxwell Tidou Abiba Sanogo Abdelkarim Filali-Maltouf UFR Agro-Forestry and Environment Department of Biochemistry and MicrobiologyLaboratory of AgrovalorizationJean Lorougnon Guede UniversityDaloaCote d’Ivoire National Center for Agronomic Research(CNRA) BouakéCote d’Ivoire Faculty of Sciences Laboratory of Microbiology and Molecular BiologyUniversity Mohammed V-AgdalRabatMorocco

Soybean [Glycine max (L.) Merrill] is an important crop known to improve population nutritional status and increase soil fertility and its productivity through biological nitrogen fixation. In C?te d’Ivoire, the introduced Bradyrhizobium japonicum used as inoculum had slight compatibility to several soybean varieties compromising their vulgarization. Therefore, the present study was conducted to examine the infectiveness and the effectiveness of indigenous rhizobial isolates on three soybean varieties (Canarana, Doko and Piramana) cultivated in C?te d’Ivoire. The experiment was conducted with potted plant filled with sterilized sand and was statistically laid in Completely Randomized Design (CRD) with sixteen (16) natives rhizobia, one (01) reference strain and uninoculated control (with or without nitrogen) with three replications. The results showed that inoculation significantly improved nodule number, nodule dry weight, plant height and total dry matter of soybean over the negative control treatment. Among the inoculated treatments, five indigenous rhizobia RSC119, RSC324, RSC502, RSC504 and RSC508 significantly (P -1) compared to IRAT FA3 strain (95 mg·plant-1) on Doko. The higher effectiveness was recorded with RSC119, RSC504 and RSC502 with 206.73%;201.79% and 200.45% respectively compared to TN (100%). The correlation analysis indicated significant association of nodule number and total biomass indicating the importance of symbiotic nitrogen fixation. Based on their infectiveness and their effectiveness, the isolates RSC119, RSC324, RSC502, RSC504 and RSC508 could be used as elite local rhizobia and tested in field conditions to establish their potential contribution on soybean productivity.

关键词： Selection Indigenous Rhizobia Inoculation Soybean Cote d’Ivoire

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An Inventory of Some Relatively Large Marine Mammals, Reptiles, and Fishes Sighted, Caught, By-Caught, or Stranded in the Mediterranean Coast of the Gaza Strip-Palestine

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Open Journal of Ecology 2023年第2期13卷 119-153页

作者： Abdel Fattah N. Abd Rabou Kamal E. Elkahlout Kamal J. Elnabris Adel J. Attallah Jehad Y. Salah mohammed A. Aboutair Waleed M. Thabit Sahar K. Serri Hassan G. Abu Hatab Sameeh M. Awadalah Wajdi M. Saqallah Mokhles S. Alhawajri Tariq K. Al-Sammak Bashar S. Jarayseh Sinaa A. Ababsa Daoud I. Al-Hali Aysha A. Rafeea Doaa S. Ghattas Huda E. Abu Amra Israa M. Jaber Nedal A. Fayyad Ashraf A. Shafei Inas A. Abd Rabou Othman A. Abd Rabou mohammed A. Abd Rabou Mazen T. Abualtayef mohammed R. Al-Agha Yousif K. ibrahim Rimel M. Benmessaoud Mourad M. Cherif Hashem A. Madkour Department of Biology and Biotechnology Faculty of Science Islamic University of Gaza Gaza Strip Palestine Department of Marine Sciences Faculty of Science Islamic University of Gaza Gaza Strip Palestine Directorate General of Fisheries Ministry of Agriculture Gaza Strip Palestine Environmental Education Center Beit Jala Palestine Wildlife Documenter and Photographer Kafr Kanna Palestine Qimmah Secondary School Jerusalem Palestine Kamal Al-Ohood Primary School for Girls North Gaza Education Directorate Gaza Strip Palestine Department of Biology Faculty of Science Al-Aqsa University Gaza Strip Palestine Department of Nursing and Health Sciences University College of Applied Sciences Gaza Strip Palestine Department of Journalism and Media Islamic University of Gaza Gaza Strip Palestine Department of GIS University College of Applied Sciences Gaza Strip Palestine Department of Environmental Engineering Faculty of Engineering Islamic University of Gaza Gaza Palestine Department of Environment and Earth Sciences Faculty of Science Islamic University of Gaza Gaza Palestine Water and Environment Quality Authority Gaza Strip Palestine National Institute of Agronomy of Tunisia Tunis Tunisia National Institute of Marine Sciences and Technologies (INSTM) La Goulette Port Tunis Tunisia National Institute of Oceanography and Fisheries Hurghada Egypt

The Mediterranean Sea is home to an interesting biodiversity. The current cumulative and descriptive study aims to enumerate some relatively large marine mammals, reptiles, and fishes that have been spotted, caught, by-caught, or stranded on the coast of the Gaza Strip, Palestine, which extends about 42 km along the Mediterranean Sea. This 20-year study from 2003 to 2022 relied much on frequent field visits, meetings, and discussions with stakeholders, following local media sites and social media pages, and photography. The study recorded at least 26 giants or relatively large marine organisms on the Mediterranean coast of the Gaza Strip, Palestine. Certainly, the coming years may bring other marine organisms of relatively large sizes. Marine mammals included three species of cetaceans with the Fin Whale (Balaenoptera physalus) being the largest mammal and even the largest animal ever recorded in this study. Marine reptiles included three species of sea turtles, the largest of which is the Leatherback Sea Turtle (Dermochelys coriacea), which is in fact the world’s largest sea turtle. The bony fishes were represented by ten species, the largest of which was the Ocean Sunfish (Mola mola), which is the largest bony fish in the world. The cartilaginous fishes included ten species with the largest specimens encountered were the Shortfin Shark (Isurus oxyrinchus), Bluntnose Sixgill Shark (Hexanchus griseus), Scalloped Hammerhead Shark (Sphyrna lewini), and Giant Devil Ray (Mobula mobular). Seabirds were not included in the study. The Alexandria Pompano (Alectis alexandrinus) and the Silver-Cheeked Toadfish (Lagocephalus sceleratus), which are bony fishes, appear to be the relatively smallest marine organisms recorded here. In conclusion, the role of the various parties must be coordinated to ensure the sustainability of human activities and their compatibility with the task of conserving local marine biota, including the gigantic or relatively large ones.

关键词： Mediterranean Sea Marine Biodiversity Marine Mammals Sea Turtles Bony Fishes Cartilaginous Fishes Gaza Strip Palestine

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Youth’s Role in Peace Building:A Case Study of Gadarif State

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Sociology Study 2024年第5期14卷 248-261页

作者： Islam mohammed Abdalla Eisa Saad Mohamed Abdal Karem Abakar University of Genina Sudan University of Gadarif Sudan

This study in Gadarif State,Sudan,analyzes the crucial role of youth in conflict resolution and peace building efforts amidst the ongoing *** reveal that youth,facing economic,environmental,and security challenges,actively engage in peacemaking through initiatives,emergency rooms,and cultural programs,contributing to social cohesion and combatting hate *** their willingness to build peace,they encounter obstacles like the lack of training programs,limited support,and marginalization in policy-making *** emphasize the necessity of empowering youth through training programs,enhancing government support,and addressing negative perceptions from older generations towards youth ***,prioritizing youth issues and fostering their participation in peace building initiatives are essential for sustainable peace and social harmony in Gadarif State.

关键词： peace building peace issues youth contributions youth initiatives social cohesion young people community emergency rooms

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