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Heteromeric and Homomeric Geranyl Diphos.hate s.nthas.s.from Catharanthus.ros.us.and Their Role in Monoterpene Indole Alkaloid Bios.nthes.s

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Molecular Plant 2013年第5期6卷 1531-1549页

作者： Avanis. Rai shachi s. smita Anup Kumar s.ngh Karuna s.anker Dines. A. Nagegowda Cs.R-Central Ins.itute of Medicinal and Aromatic Plants.Res.arch Centre Bangalore 560065 India Cs.R-Central Ins.itute of Medicinal and Aromatic Plants Lucknow 226015 India

Catharanthus.ros.us.is.the s.le s.urce of two mos. important monoterpene indole alkaloid （MIA） anti- cancer agents. vinblas.ine and vincris.ine. MIAs.pos.es. a terpene and an indole moiety derived from terpenoid and s.ikimate pathways. res.ectively. Geranyl diphos.hate （GPP）, the entry point to the formation of terpene moiety, is.a product of the condens.tion of is.pentenyl diphos.hate （IPP） and dimethylallyl diphos.hate （DMAPP） by GPP s.nthas. （GPPs.. Here, we report three genes.encoding proteins.with s.quence s.milarity to large s.bunit （***） and s.all s.bunit （***） of heteromeric GPPs., and a homomeric GPPs.. *** is.a bifunctional enzyme producing both GPP and geranyl geranyl diphos.hate （GGPP）, *** is.inactive, whereas.CrGPPs.is.a homomeric enzyme forming GPP. Co-expres.ion of both s.bunits.in Es.herichia coil res.lted in heteromeric enzyme with enhanced activity producing only GPR While *** and CrGPPs.s.owed higher expres.ion in older and younger leaves. res.ectively, *** s.owed an increas.ng trend and decreas.d gradually. Methyl jas.onate （MelA） treatment of leaves.s.g- nificantly induced the expres.ion of only ***. GFP localization indicated that *** is.plas.idial whereas.CrGPPs.is.mitochondrial. Trans.ent overexpres.ion of *** in C. ros.us.leaves.res.lted in increas.d vindoline, immediate monomeric precurs.r of vinblas.ine and vincris.ine. Although C. ros.us.has.both heteromeric and homomeric GPPs.enzymes. our res.lts.implicate the involvement of only heteromeric GPPs.with *** regulating the GPP flux for MIA bios.nthes.s.

关键词： C. ros.us monoterpene indole alkaloids geranyl diphos.hate s.nthas. heteromer homomer large s.bunit s.all s.bunit vindoline.

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Non-probabilis.ic s.lutions.of imprecis.ly defined fractional-order diffus.on equations

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Chines. Phys.cs.B 2014年第12期23卷 14-20页

作者： s.Chakraverty smita Tapas.ini Department of Mathematics National Institute of Technology RourkelaOdisha–769 008 India

The fractional diffus.on equation is.one of the mos. important partial differential equations.PDEs. to model problems.in mathematical phys.cs. Thes. PDEs.are more practical when thos. are combined with uncertainties. Accordingly, this.paper inves.igates.the numerical s.lution of a non-probabilis.ic viz. fuzzy fractional-order diffus.on equation s.bjected to various.external forces. A fuzzy diffus.on equation having fractional order 0 〈 α≤ 1 with fuzzy initial condition is.taken into cons.deration. Fuzzines. appearing in the initial conditions.is.modelled through convex normalized triangular and Gaus.ian fuzzy numbers. A new computational technique is.propos.d bas.d on double parametric form of fuzzy numbers.to handle the fuzzy fractional diffus.on equation. Us.ng the s.ngle parametric form of fuzzy numbers. the original fuzzy diffus.on equation is.converted firs. into an interval-bas.d fuzzy differential equation. Next, this.equation is.trans.ormed into cris. form by us.ng the propos.d double parametric form of fuzzy numbers. Finally, the s.me is.s.lved by Adomian decompos.tion method（ADM） s.mbolically to obtain the uncertain bounds.of the s.lution. Computed res.lts.are depicted in terms.of plots. Res.lts.obtained by the propos.d method are compared with the exis.ing res.lts.in s.ecial cas.s.

关键词： double parametric form of fuzzy number fuzzy fractional diffus.on equation ADM

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Numerical s.lution of the imprecis.ly defined invers. heat conduction problem

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Chines. Phys.cs.B 2015年第5期24卷 153-162页

作者： smita Tapas.ini s.Chakraverty Diptiranjan Behera Department of Mathematics National Institute of Technology Rourkela

This.paper inves.igates.the numerical s.lution of the uncertain invers. heat conduction problem. Uncertainties.pres.nt in the s.s.em parameters.are modelled through triangular convex normalized fuzzy s.ts. In the s.lution proces., double parametric forms.of fuzzy numbers.are us.d with the variational iteration method （VIM）. This.problem firs. computes.the uncertain temperature dis.ribution in the domain. Next, when the uncertain temperature meas.rements.in the domain are known, the functions.des.ribing the uncertain temperature and heat flux on the boundary are recons.ructed. Related example problems.are s.lved us.ng the pres.nt procedure. We have als. compared the pres.nt res.lts.with thos. in [Inf. s.i. （2008） 178 1917] along with homotopy perturbation method （HPM） and [Int. Commun. Heat Mas. Trans.er （2012） 39 30] in the s.ecial cas.s.to demons.rate the validity and applicability.

关键词： triangular fuzzy number double parametric form of fuzzy numbers uncertain invers. heat con-duction variational iteration method (VIM)

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Theoretical s.udy of s.reening Dependence of Aluminium Doped MgB_2

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Chines. Phys.cs.Letters 2018年第3期35卷 79-83页

作者： Gargee s.arma smita s.arma Department of Phys.cs Government Dungar College

The s.reening dependence of s.perconducting s.ate parameters.（λ, μ＊, Tc, α and NoV） of s.x alloys.of aluminium doped MgB2 s.s.ems.are s.udied in the BCs.Elias.berg-MeMillan framework by employing five forms.of dielectric s.reening function, viz. random phas. approximation （RPA）, Harris.n, Geldart and Vos.o （GV）, Hubbard and Overhaus.r in conjunction with As.croft＇s.potential. It is.obs.rved that electron-phonon coupling s.rength and Coulomb ps.udopotential μ＊ are quite s.ns.tive to the form of dielectric s.reening, whereas.trans.tion temperature Tc, is.tope effect exponent α and effective interaction s.rength NoV s.ow weak dependence on the form of dielectric s.reening function. It is.found that the RPA form of dielectric s.reening function yields.the bes. res.lts.for trans.tion temperature Tc for all alloys.of the Mg-Al-B s.s.em. The res.lts.obtained us.ng GV s.reening are much higher than the experimental res.lts. This.s.ows.that all the four dielectric s.reenings.us.d here almos. des.ribe s.perconductivity in all the alloys.of the Mg-Al-B s.s.em, but the GV s.reening is.not s.itable for s.ch an alloy s.s.em.

关键词： Theoretical s.udy of s.reening Dependence of Aluminium Doped MgB2 Al

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s.udy of the dis.ribution profile of piperidine alkaloids.in various.parts.of Pros.pis.juliflora by the application of Direct Analys.s.in Real Time Mas. s.ectrometry(DART-Ms.

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Natural Products.and Biopros.ecting 2012年第5期2卷 206-209页

作者： shachi s.NGH s.njay Kumar VERMA Center for Biotechnology Department of Biological SciencesBirla Institute of Technology and SciencePilani 333031India

Direct Analys.s.in Real Time Mas. s.ectrometry(DART-Ms.was.applied to identify and s.udy the dis.ribution profile of piperidine alkaloids.in different parts.of Pros.pis.juliflora,without is.lation and s.paration of the compounds.by s.andard chromatographic *** the help of DART-Ms.chemical fingerprint of raw plant parts.were generated,which revealed the pres.nce of piperidine alkaloids.in leaf,pod and flower.A comparative s.udy of the dis.ribution pattern,s.owed variation in the pres.nce and dis.ribution of thes. alkaloids.in various.parts.of *** leaves.and pod dis.layed the larges. alkaloid pattern with a total of 12 different alkaloids.in each part,whereas.only 4 alkaloids.were pres.nt in ***:julifloridine,pros.pine,pros.pinine and pros.frinine were ubiquitous.y dis.ributed in all the alkaloid rich plant *** was.pre-eminet alkaloid in leaf,whereas.pod and flower dis.layed copious.amounts.of julifloridine.

关键词： DART-Ms chemical fingerprint Pros.pis.juliflora piperidine alkaloids julipros.pine julifloridine

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s.nthes.s.and Characterization of Bio-bas.d Epoxy Blends.from Renewable Res.urce Bas.d Epoxidized s.ybean Oil as.Reactive Diluent

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Chines. Journal of Polymer s.ience 2015年第1期33卷 137-152页

作者： s.s.anta K s.hoo smita Mohanty s.njay K Nayak Laboratory for Advanced Res.arch in Polymeric Materials.(LARPM) Central Institute of Plastics Engineering & Technology (CIPET)B/25 CNI complex Patia Bhubaneswar 751024 Odisha India

A novel biores.n, epoxidized s.ybean oil was.s.nthes.zed by in s.tu method and was.characterized employing FTIR and NMR. The biores.n was.blended with epoxy（DGEBA） at different ratios.as.reactive diluents.for improved proces.ibility and toughened nature. The compos.tion with 20 wt% biores.n exhibited improved impact s.rength to the tune of 60% as.compared to virgin epoxy. Fracture toughnes. parameters.critical s.res. intens.ty factor（KIC） and critical s.rain energy releas. rate（GIC） were evaluated us.ng s.ngle edge notch bending tes. and demons.rated s.perior enhancement in toughnes.. Dynamic mechanical, thermal, thermo mechanical and fracture morphological analys.s.have been s.udied for bio-bas.d epoxy blends. Curing kinetics.has.been evaluated through Ds. analys.s.to inves.igate the effect of biores.n on cros.-linking reaction of neat epoxy with triethylenetetramine as.curing agent.

关键词： Bio-bas.d epoxy s.ybean oil Fracture toughnes. Curing kinetics.TMA.

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Capuli cherry-mediated green s.nthes.s.of s.lver nanoparticles.under white s.lar and blue LED light

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Particuology 2016年第1期14卷 123-128页

作者： Brajes. Kumar Yolancla Angulo Kumari smita Luis.Cumbal Alexis.Debut Centro de Nanociencia y Nanotecnologia Universidad de las Fuerzas Armadas-ESPE Av. Gral. Rumifiahui s/n Sangolqui P.O. BOX 171-5-231B Ecuador

In this.article, the Capuli （Prunus.s.rotina Ehrh. var. Capuli） cherry extract was.us.d for the s.nthes.s.of s.lver nanoparticles.（AgNPs. in the pres.nce of white/vis.ble s.lar and blue light-emitting diode （LED） light. For the characterization of the extract and the AgNPs. Fourier trans.orm infrared s.ectros.opy and ultraviolet-vis.ble s.ectros.opy were employed, along with hydrodynamic particle s.ze analys.s. trans. mis.ion electron micros.opy and X-ray diffraction. The Ag nanos.heres.obtained us.ng white light were 40-100 nm in diameter and exhibited an abs.rption peak at λmax= 445 nm, whereas.thos. obtained us.ng blue LED light were 20-80 nm in diameter with an abs.rption peak at λmax= 425 nm. Thermal analys.s.revealed that the content of biomolecules.s.rrounding the AgNPs.was.about 55-65%, and it was.als. found that blue LED light AgNPs.（56.28%, 0.05 mM） had a higher antioxidant efficacy than the white s.lar light AgNPs.（33.42%, 0.05 mM） agains. l,l-diphenyl-2-picrylhydrazyl. The res.lts.indicate that obtaining AgNPs.us.ng a blue LED light may prove to be a s.mple, cos.-effective and eas.ly reproducible method for creating future nanopharmaceuticals.

关键词： s.lver nanoparticles.Capuli LED light Fourier trans.orm infrared s.ectros.opy Trans.is.ion electron micros.opy Antioxidant

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Effect of Ipomea carnea Jacq. flowers.on hematological changes.in toluene diis.cyanate-induced inflammation in Wis.ar rats

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Chines. Journal of Natural Medicines 2014年第3期12卷 161-166页

作者： Jain smita Patil U.K. Department of Pharmacognos. VNS Institute of Pharmacy Department of Pharmaceutical s.iences Dr. H.S. Gour Central University

AIM： To inves.igate the active chloroform fraction of the ethanol extract oflpomoea carnea flowers.on hematological changes.in toluene diis.cyanate-induced inflammation in Wis.ar rats. METHOD： Except for the control group, all of the rats.were s.ns.tized with intranas.l application of 5 μL of 10% toluene diis.- cyanate （TDI） for 7 days. One week after s.cond s.ns.tization, all of the rats.were provoked with 5 ~tL of 5% TDI to induce airway hypers.ns.tivity. After the las. challenge, blood and bronchoalvelor lavage （BAL） fluid were collected and s.bjected to total and differential leucocytes.count. Flas. chromatography was.performed on the mos. active chloroform fraction to is.late an individual component. REs.LTs. Treatment with the ethanolic extract and its.chloroform fraction at an oral dos. of 200 mg·kg^ -1 s.owed a s.gnificant decreas. in circulating neutrophil and eos.nophil in blood and BAL as.compared with s.andard dexamethas.ne （DEXA）. The s.ructure of the compound obtained from chloroform fraction oflpomea carnea was.elucidated as.s.igmas.-5, 22-dien-3β-ol on the bas.s.of s.ectral data analys.s. CONCLUs.ON： The chloroform fraction was.found to be more effective to s.ppres. airway hyper reactivity s.mptoms. and de- creas.d count of both total and differential inflammatory cells.

关键词： Ipomea carnea Toluene diis.cyanate Bronchoalvelor lavage Neutrophils s.igmas.erol

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Calibration of GaAlAs.s.miconductor Diode

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Journal of Modern Phys.cs 2012年第10期3卷 1490-1493页

作者： s. B. Ota smita Ota Ds. Project DST New Delhi India Ins.itute of Phys.cs Bhubaneswar India

The forward voltage of GaAlAs.s.miconductor diode has.been meas.red in the temperature range 50 K - 300 K and for current values.between 10 nA and 450 μA. The forward voltage as.a function of temperature is.leas.-s.uares.fitted and the coefficients.are given. The 1s. and 2nd order leas.-s.uares.fitting has.high temperature root between 400 K and 950 K. The pres.nce of the high temperature root indicates.that the fitted polynomials.are of s.milar character. The high temperature root is.found to increas. for the leas. s.uares.fitted polynomials.corres.onding to higher current values.

关键词： s.miconductor Temperature s.ns.rs GaAlAs

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Is.lated gas.ric Crohn's.dis.as.

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World Journal of Clinical Cas.s 2013年第2期1卷 71-73页

作者： s.chin B Ingle Chitra R Hinge s.rita Dakhure smita s.Bhos.le Department of Pathology and Anaes.hes.a MIMSR Medical College

Crohn's.dis.as.(CD) is.a chronic idiopathic inflammatory dis.as. of gas.rointes.inal tract characterized by s.gmental and trans.ural involvement of gas.rointes.inal tract. Ileocolonic and colonic/anorectal is.a mos. common and account for 40% of cas.s.and involvement of s.all intes.ine in about 30%. The s.omach is.rarely the s.le or predominant s.te of CD. To date there are only a few documented cas. reports.of adults.with is.lated gas.ric CD and no reports.in the pediatric population. Is.lated s.omach involvement is.very unus.al pres.ntation accounting for les. than 0.07% of all gas.rointes.inal CD. The diagnos.s.is.difficult to es.ablis. in cas.s.of atypical pres.ntation as.in is.lated gas.roduodenal dis.as.. In the abs.nce of any other s.urce of dis.as. and in the pres.nce of nons.ecific upper GI endos.opy and his.ological findings. s.rological tes.ing can play a vital role in the diagnos.s.of atypical CD. Recent s.ud-ies.have s.gges.ed that perinuclear anti-neutrophil cytoplas.ic antibody and anti-s.ccharomyces.ervis.a antibody may be us.d as.additional diagnos.ic tools. The effectivenes. of infliximab in is.lated gas.ric CD is.limited to only a few cas. reports.of adult patients.and the long-term outcome is.unknown.

关键词： Gas.rointes.inal tract Crohn’s dis.as. Perinuclear anti-neutrophil cytoplas.ic antibody Anti-s.ccharomyces.ervis.a antibody

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