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Comparison of intracytoplasmic sperm injection(ICSI)outcomes in infertile men with spermatogenic impairment of differing severity

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Asian Journal of Andrology 2022年第3期24卷 299-304页

作者： Ping Ping Zhong Zheng Yi Ma sha-sha zou Xiang-Feng Chen Center for Reproductive Medicine Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200135China shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai 200135China shanghai Human Sperm Bank Shanghai 200135China

The extent of spermatogenic impairment on intracytoplasmic sperm injection(ICSI)outcomes and the risk of major birth defects have been little *** this study,we evaluated the relationship between various spermatogenic conditions,sperm origin on ICSI outcomes,and major birth defects.A total of 934 infertile men attending the Center for Reproductive Medicine of Ren Ji Hospital(shanghai,China)were classified into six groups:nonobstructive azoospermia(NOA;n=84),extremely severe oligozoospermia(esOZ;n=163),severe oligozoospermia(sOZ,n=174),mild oligozoospermia(mOZ;n=148),obstructive azoospermia(OAZ;n=155),and normozoospermia(NZ;n=210).Rates of fertilization,embryo cleavage,high-quality embryos,implantation,biochemical and clinical pregnancies,abortion,delivery,newborns,as well as major birth malformations,and other newborn outcomes were analyzed and compared among *** NOA group showed a statistically lower fertilization rate(68.2%vs esOZ 77.3%,sOZ 78.0%,mOZ 73.8%,OAZ 76.6%,and NZ 79.3%,all P<0.05),but a significantly higher implantation rate(37.8%)than the groups esOZ(30.1%),sOZ(30.4%),mOZ(32.6%),and OAZ(31.0%)(all P<0.05),which was similar to that of Group NZ(38.4%).However,there were no statistically significant differences in rates of embryo cleavage,high-quality embryos,biochemical and clinical pregnancies,abortions,deliveries,major birth malformations,and other newborn outcomes in the six *** results showed that NOA only negatively affects some embryological outcomes such as fertilization *** was no evidence of differences in other embryological and clinical outcomes with respect to sperm source or spermatogenic *** failure and sperm origins do not impinge on the clinical outcomes in ICSI treatment.

关键词： intracytoplasmic sperm injection nonobstructive azoospermia normozoospermia obstructive azoospermia oligozoospermia

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A novel micro-straw for cryopreservation of sma number of human spermatozoon

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Asian Journal of Andrology 2017年第3期19卷 326-329页

作者： Feng Liu sha-sha zou Yong Zhu Can Sun Yu-Fei Liu shan-shan Wang Wen-Bo Shi Jing-Jing Zhu Yong-Hua Huang Zheng Li Department of Andrology Center for Men's Health Shanghai China Urologic Medical Center Shanghai General Hospital Shanghai Jiao Tong University Shanghai China Center for Reproductive Medicine Ren Ji Hospital School of Medicine Shanghai Jiao Tong University Shanghai China shanghai Human Sperm Bank. shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai China Institute of Refrigeration and Cryogenics School of Mechanical Engineering Shanghai Jiao Tong University Shanghai China shanghai Key Laboratory of Reproductive Medicine Shanghai China

Cryopreservation of few spermatozoa is still a major challenge for male fertility preservation. This study reports use a new micro-straw （LSL straw） for freezing few spermatozoa for intracytoplasmic sperm injection （ICSI）. Semen samples from 22 fertile donors were collected, and each semen sample was diluted and mixed with cryoprotectant in a ratio of 1：1, and then frozen using three different straws such as LSL straw （50-100μl）, traditional 0.25 ml and 0.5 ml straws. For freezing, all straws were fumigated with liquid nitrogen, with temperature directly reducing to -130--140℃. Sperm concentration, progressive motility, morphology, acrosome integrity, and DNA fragmentation index were evaluated before and after freezing. After freezing-thawing, LSL straw group had significantly higher percentage of sperm motility than traditional 0.25 ml and 0.5 ml straw groups （38.5% vs 27.4% and 25.6%, P 〈 0.003）. Sperm motility and acrosomal integrity after freezing-thawing were significantly lower than that of before freezing. However, there was no significant difference in morphology, acrosome, and DNA integrity between the three types of straws （P 〉 0.05）. As LSL straws were thinner and hold very small volume, the freezing rate of LSL straw was obviously faster than 0.25 ml straw and 0.5 ml straws. In conclusion, LSL micro-straws may be useful to store few motile spermatozoa with good recovery of motility for patients undergoing ICSI treatment.

关键词： cryopreservation freezing rate LSL straw micro-straw motility

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Detection of Endocrine Disruptors in Water around Landfills

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Agricultural Biotechnology 2024年第1期13卷 66-70,73页

作者： Dandan LIU Qing LI Yaohong LIU sha FENG Tao MO Zheng LIU Xiaonan zou Guangzhou GRG Metrology&Test(Hunan)Co. Ltd.Changsha 410007China Anhua Administration for Market Regulation Yiyang 413500China Guizhou Engineering Vocational College Tongren 565200China

[Objectives]This study was conducted to explore the occurrence levels of endocrine disruptors(EDCs)in rural areas around a county landfill in Tongren City.[Methods]The water around the landfill was sampled and analyzed.A solid-phase extraction and high performance liquid chromatography-tandem mass spectrometry(SPE-UPLC-MS/MS)method was established for the determination of 27 *** the HLB solid-phase extraction column was activated,a water sample,which was adjusted with phosphoric acid to a pH of 2(±0.5)and added with 500 mg of disodium EDTA,was loaded,and 5 ml of water and 20%methanol water was added for ***,10 ml of elution solution was added for elution,and the collected eluate was evaporated under reduced pressure at 40℃to near dryness,and 1 ml of reconstitution solution was added to a constant *** ACQUITY UPLC BEH C18(100×2.1 mm,2.6μm)chromatographic column was adopted for LC separation by gradient elution with pure water solution-acetonitrile as the mobile *** MS detection,the MRM mode was adopted for collection,and the positive and negative ion modes were switched for simultaneous determination,and the internal standard method was used for quantification.[Results]The correlation coefficient R2 was greater than 0.99 in the linear range of each target *** limits of quantitation in the method were between 0.05 and 2.00 ng/L,and the recoveries ranged from 75.3%to 105.7%.[Conclusions]The method has high sensitivity,good accuracy and strong practical value.

关键词： Landfill Endocrine disruptor Solid phase extraction High performance liquid chromatography-tandem mass spectrometry

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Study on Detection of Antibiotic Contents in Water around Landfill Sites

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Agricultural Biotechnology 2024年第2期13卷 33-37页

作者： sha FENG Dandan LIU Juan LUO Qing LI Tao MO Zheng LIU Xiaonan zou Guangzhou GRG Metrology&Test(Hunan)Co. Ltd.Changsha 410007China Anhua Administration for Market Regulation Yiyang 413500China Guizhou Engineering Vocational College Tongren 565200China

[Objectives] An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for simultaneous determination of 26 antibiotics in the water around landfills. [Methods] After an HLB solid-phase extraction column was activated, and a water sample, which was adjusted with phosphoric acid to a pH of (2±0.5) and added with 500 mg of disodium EDTA, was loaded, and 5 ml of water and 20% methanol water was added for washing. Next, 10 ml of elution solution was added for elution, and the collected eluate was evaporated under reduced pressure at 40 ℃ to near dryness, and 1 ml of reconstitution solution was added to a constant volume. An ACQUITY UPLC BEH C18 (100 mm×2.1 mm, 2.6 μm) chromatographic column was adopted for LC separation by gradient elution with 0.1% formic acid aqueous solution-acetonitrile as the mobile phase. For MS detection, the MRM mode was adopted for collection, and the positive and negative ion modes were switched for simultaneous determination, and the internal standard method was used for quantification. [Results] The correlation coefficient R2 was greater than 0.99 in the linear range of each target substance. The limits of detection ranged from 0.15 to 3.00 ng/L, and the limits of quantitation were between 0.80 and 10.00 ng/L, and the recoveries ranged from 77.9% to 104.85%. [Conclusions] The method has high sensitivity, good accuracy and strong practical value.

关键词： Landfill Antibiotics Ultra high performance liquid chromatography-tandem mass spectrometry

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Calculus bovis inhibits M2 tumor-associated macrophage polarization via Wnt/β-catenin pathway modulation to suppress liver cancer

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World Journal of Gastroenterology 2024年第29期30卷 3511-3533页

作者： Zhen Huang Fan-Ying Meng Lin-Zhu Lu Qian-Qian Guo Chang-Jun Lv Nian-Hua Tan Zhe Deng Jun-Yi Chen Zi-Shu Zhang Bo zou Hong-Ping Long Qing Zhou sha Tian Si Mei Xue-Fei Tian College of Integrated Chinese and Western Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Hunan Key Laboratory of Translational Research in Formulas and Zheng of Traditional Chinese Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Key Laboratory of Traditional Chinese Medicine for Mechanism of Tumor Prevention and Treatment Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina The First Clinical College of Traditional Chinese Medicine Hunan University of Traditional Chinese MedicineChangsha 410007Hunan ProvinceChina Department of Hepatology Hunan University of Chinese MedicineChangsha 410007Hunan ProvinceChina The First Hospital of Hunan University of Chinese Medicine Hunan University of Chinese MedicineChangsha 410007Hunan ProvinceChina Faculty of Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina

BACKGROUND Calculus bovis(CB),used in traditional Chinese medicine,exhibits anti-tumor effects in various cancer *** also constitutes an integral component of a compound formulation known as Pien Tze Huang,which is indicated for the treatment of liver ***,its impact on the liver cancer tumor microenvironment,particularly on tumor-associated macrophages(TAMs),is not well *** To elucidate the anti-liver cancer effect of CB by inhibiting M2-TAM polarization via Wnt/β-catenin pathway *** This study identified the active components of CB using UPLC-Q-TOF-MS,evaluated its anti-neoplastic effects in a nude mouse model,and elucidated the underlying mechanisms via network pharmacology,transcriptomics,and molecular *** vitro assays were used to investigate the effects of CB-containing serum on HepG2 cells and M2-TAMs,and Wnt pathway modulation was validated by real-time reverse transcriptase-polymerase chain reaction and Western blot *** This study identified 22 active components in CB,11 of which were detected in the *** investigations have demonstrated the ability of CB to effectively inhibit liver tumor *** integrated approach employing network pharmacology,transcriptomics,and molecular docking implicated the Wnt signaling pathway as a target of the antineoplastic activity of CB by suppressing M2-TAM *** vitro and in vivo experiments further confirmed that CB significantly hinders M2-TAM polarization and suppresses Wnt/β-catenin pathway *** inhibitory effect of CB on M2-TAMs was reversed when treated with the Wnt agonist SKL2001,confirming its pathway *** This study demonstrated that CB mediates inhibition of M2-TAM polarization through the Wnt/β-catenin pathway,contributing to the suppression of liver cancer growth.

关键词： Calculus bovis M2 tumor-associated macrophage polarization Liver cancer Wnt/β-catenin pathway Tumor microenvironment

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中国高口感品质鲜食辣椒产业发展与未来趋势

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园艺学报 2024年第1期51卷 27-38页

作者：邹学校杨莎朱凡远方湖南农业大学园艺作物种质创新与新品种选育教育部工程研究中心长沙410128

鲜食辣椒的品质包括营养、口感和外观等。在食物十分丰富、营养充足的当下人们更加关注口感品质。目前口感好的薄皮泡椒、螺丝椒和嫩果型鲜食辣椒成为中国辣椒产业新的增长点和市场的热点。本文中介绍了这3种辣椒的特点,采收标准,优异... 详细信息

鲜食辣椒的品质包括营养、口感和外观等。在食物十分丰富、营养充足的当下人们更加关注口感品质。目前口感好的薄皮泡椒、螺丝椒和嫩果型鲜食辣椒成为中国辣椒产业新的增长点和市场的热点。本文中介绍了这3种辣椒的特点,采收标准,优异种质资源,育种历史、现状与成果,市场需求与存在问题;提出建立辣椒高端品牌、实现辣椒适度规模生产和选用辣椒加工专用品种是中国辣椒产业未来高质量发展趋势。

关键词：辣椒鲜食口感品质高品质辣椒产业高质量发展

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二氧化碳电催化还原酸性体系研究进展

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材料导报 2024年第8期38卷 51-56页

作者：孙亚洲徐沙邹金含吴智华谢顺吉厦门大学化学化工学院固体表面物理化学国家重点实验室能源材料化学协同创新中心福建能源材料科学与技术创新实验室福建厦门361005

利用可再生能源提供的电能电催化还原CO_(2)制高值化学品和燃料,为全球气候变暖和能源危机提供了一种可行的解决方案,具有巨大的实际应用潜力。目前研究较多的碱性和中性反应体系中CO_(2)易与氢氧根(OH^(-))反应生成碳酸盐或碳酸氢盐,造... 详细信息

利用可再生能源提供的电能电催化还原CO_(2)制高值化学品和燃料,为全球气候变暖和能源危机提供了一种可行的解决方案,具有巨大的实际应用潜力。目前研究较多的碱性和中性反应体系中CO_(2)易与氢氧根(OH^(-))反应生成碳酸盐或碳酸氢盐,造成CO_(2)损失的同时会降低反应体系的能量转化效率和稳定性。研究开发酸性体系的电催化还原CO_(2)反应(CO_(2)RR),有望解决上述问题。本文针对CO_(2)电催化还原的酸性体系展开讨论,系统整理了反应装置、催化剂、反应微环境对CO_(2)RR性能的影响以及酸性体系中的微观反应机理,最后针对酸性体系中催化剂设计、反应机理研究、反应微环境调控、反应器设计优化等提出前瞻性建议。

关键词：二氧化碳电催化还原酸性电解体系

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社会排斥对跨期决策的影响:自我控制的中介作用和归属需求的调节作用

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中国临床心理学杂志 2024年第4期32卷 908-911,842页

作者：杨晓莉冯琳邹妍刘晶莎西北师范大学心理学院甘肃省行为与心理健康重点实验室兰州730070

目的:探讨社会排斥对跨期决策的影响,以及自我控制和归属需求在二者关系中的作用。方法:以265名大学生为被试,采用单因素被试间实验设计,自变量为情景类型(社会排斥/社会接纳),因变量为跨期决策时间折扣率,中介变量为状态自我控制,调节... 详细信息

目的:探讨社会排斥对跨期决策的影响,以及自我控制和归属需求在二者关系中的作用。方法:以265名大学生为被试,采用单因素被试间实验设计,自变量为情景类型(社会排斥/社会接纳),因变量为跨期决策时间折扣率,中介变量为状态自我控制,调节变量为归属需求。结果:(1)社会排斥正向预测时间折扣率;(2)自我控制在社会排斥和时间折扣率间起部分中介作用;(3)归属需求在社会排斥影响自我控制的路径中起调节作用。在被排斥个体中,相较于低归属需求的个体,高归属需求个体的自我控制资源下降得更多。结论:社会排斥会导致自我控制资源的下降,进而导致短视决策,但这一路径会因为个体归属需求的差异而不同。

关键词：社会排斥自我控制归属需求跨期决策

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复合式小梁切除术联合透明质酸钠治疗青光眼41例

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国际眼科杂志 2006年第5期6卷 1158-1159页

作者：邹莎莎温焱马月霄中国河北省保定市第一医院眼科 071000

目的:探讨复合式小梁切除术联合透明质酸钠对防止青光眼术后并发症的影响。方法:采用41例45眼行复合式滤过术联合术中前房及巩膜瓣下注入适量的透明质酸钠的方法治疗青光眼。结果:与术前比较眼压均明显下降(P<0.001),术后浅前房I度为10... 详细信息

目的:探讨复合式小梁切除术联合透明质酸钠对防止青光眼术后并发症的影响。方法:采用41例45眼行复合式滤过术联合术中前房及巩膜瓣下注入适量的透明质酸钠的方法治疗青光眼。结果:与术前比较眼压均明显下降(P<0.001),术后浅前房I度为10眼,浅Ⅱ度3眼,绝大多数病例眼压控制满意。结论:复合式小梁切除术联合透明质酸钠可提高青光眼滤过术成功率和满意度,减少并发症的发生。

关键词：复合式滤过术透明质酸钠青光眼

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稀土元素Lu,Sc掺杂GaN光电特性的第一性原理研究

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原子与分子物理学报 2024年第1期41卷 161-167页

作者：付莎莎肖清泉姚云美邹梦真谢泉贵州大学大数据与信息工程学院新型光电子材料与技术研究所贵阳550025

基于密度泛函理论,采用广义梯度近似(GGA+U)平面波超软赝势方法,计算了本征GaN和稀土元素Lu、Sc掺杂GaN体系的电子结构和光学性质.结果表明:计算得到本征GaN的禁带宽度为3.37 eV,与实验值(3.39 eV)接近. Lu掺杂后GaN体系带隙变窄,而Sc... 详细信息

基于密度泛函理论,采用广义梯度近似(GGA+U)平面波超软赝势方法,计算了本征GaN和稀土元素Lu、Sc掺杂GaN体系的电子结构和光学性质.结果表明:计算得到本征GaN的禁带宽度为3.37 eV,与实验值(3.39 eV)接近. Lu掺杂后GaN体系带隙变窄,而Sc掺杂后诱导了深能级杂质,带隙变宽,但仍为直接带隙半导体.掺杂后体系均发生畸变,晶格常数和体积增大,且在费米能级附近产生杂质带. Lu、Sc掺杂GaN体系的静态介电常数较本征GaN(4.50)均有所增大.Lu、Sc掺杂后体系介电常数虚部整体左移,光吸收边往低能方向移动,发生了红移现象.计算结果对稀土元素Lu、Sc掺杂GaN高压光电材料的开发和研究提供了理论依据.

关键词： GaN 第一性原理掺杂光电性质

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