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Rapid Detection of Sildenafil Drugs in Liquid Nutraceuticals Based on Surface-Enhanced Raman Spectroscopy Technology

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Chinese Journal of Chemistry 2017年第10期35卷 1522-1528页

作者： Hang Zhao Wuliji Hasi Lin Bao Siqingaowa Han Xuanyu sha Jia Sun Xiutao lou Dianyang Lin Zhiwei Lv National Key Laboratory of Science and Technology on Tunable Laser Harbin Institute of Technology Harbin Heilongjiang 150080 China Affiliated HospitaI of Inner Mongolia University for the Nationalities Tongliao Inner Mongolia 028007 China

In this study, surface-enhanced Raman Spectroscopy （SERS） technology was used to rapidly detect illegally added sildenafil drugs. A detailed attribution analysis by density functional theory （DFT） was used to guide specific experiments. The Raman signals were obtained from a silver colloid （Ag col） substrate, and they increased in the presence of the mineral salt, potassium iodide （KI）. These methods detected sildenafil in aqueous solutions as low as 1 μg/mL with high signal uniformity （RSD=3.77%）. Prior to this study, traditional Raman techniques detected substances in solid samples only. Here, Raman technology detected low contents of sildenaful drugs in liquid nutraceuticals. Therefore, SERS technology has great potential for on-site and real-time detection of illegal drugs in water and in liquid nutraceuticals.

关键词： sildenafil surface-enhanced Raman spectroscopy density functional theory limit of detection

Microwave Assisted Hydrothermal Way Towards Highly Crystalized N-Doped Carbon Quantum Dots and Their Oxygen Reduction Performance

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Chemical Research in Chinese Universities 2019年第2期35卷 171-178页

作者： HUANG He LIANG Chen sha Haoyan YU Ying lou Yue CHEN Cailing LI Chunguang CHEN Xiaobo SHI Zhan FENG Shouhua State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of ChemistryJilin UniversityChangchun 130012P.R.China School of Engineering EMIT UniversityCarltonVIC 3053Australia Department of Chemical Engineering University of CaliforniaDavisCA 95616USA

We proposed a green microwave-assisted hydrothermal way to synthesize highly crystalized N-doped carbon quantum dots(N-CQDs).The N-CQDs obtained by this microwave method have good crystalline degree(ID/IG=0.6)and a high molar ratio of N/C(11.1%)comparing with those obtained from traditional top-down *** experimental results show that glycerine plays a key role ill the fonnation of highly crystalized *** as-prepared N-CQDs have good luminescent property and may be utilized as fluorescent probe to detect ions or mark *** the majority of N atoms in the N-CQDs were pyridinic type(64.8%),the as-prepared N-CQDs were used as a catalyst for the oxygen reduction reaction(ORR)electrocatalysis in the anode of the fuel cell(the onset potential is-0.121V),which was a 4e-transfer procedure and the catalyst showed good stability after 100 cycles.

关键词： Carbon-dot Nitrogen-dopping Highly-crystalized Oxygen reduction reaction(ORR)

Degradation mechanism analysis of LiNi_(0.5)Co_(0.2)Mn_(0.3)O_(2) single crystal cathode materials through machine learning

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npj Computational Materials 2022年第1期8卷 2119-2127页

作者： Wuxin sha Yaqing Guo Danpeng Cheng Qigao Han Ping lou Minyuan Guan Shun Tang Xinfang Zhang Songfeng Lu Shijie Cheng Yuan-Cheng Cao School of Computer Science and Technology Huazhong University of Science and TechnologyWuhan430074China State Key Laboratory of Advanced Electromagnetic Engineering and Technology School of Electrical and Electronic EngineeringHuazhong University of Science and TechnologyWuhan430074China Wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhan430074China State Grid Huzhou Electric Power Supply Company Huzhou313000China School of Cyber Science&Engineering Huazhong University of Science and TechnologyWuhan430074China

LiNi_(0.5)Co_(0.2)Mn_(0.3)O_(2)(NCM523)has become one of the most popular cathode materials for current lithium-ion batteries due to its high-energy density and cost ***,the rapid capacity fading of NCM severely hinders its development and ***,the single crystal NCM523 materials under different degradation states are characterized using scanning transmission electron microscopy(STEM).Then we developed a neural network model with a two-sequential attention block to recognize the crystal structure and locate defects in STEM *** number of point defects in NCM523 is observed to experience a trend of increasing first and then decreasing in the degradation *** space between the transition metal columns shrinks obviously,inducing dramatic capacity *** analysis sheds light on the defect evolution and chemical transformation correlated with layered material *** also provides interesting hints for researchers to regenerate the electrochemical capacity and design better battery materials with longer life.

关键词： battery crystal analysis

组织因子途径抑制因子/绿色荧光蛋白双顺反子真核表达载体构建及其在NIH 3T3细胞中表达

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生物医学工程与临床 2011年第2期15卷 178-182,88页

作者：宋丽萍楼莎朱敦皖刘兰霞张海玲董霞董庭婷冷希岗中国医学科学院北京协和医学院生物医学工程研究所天津市生物医学材料重点实验室天津300192 中国医学科学院基础医学研究所北京协和医学院基础医学院北京100730

目的构建含人组织因子途径抑制因子(TFPI)和绿色荧光蛋白(GFP)基因的双顺反子真核表达载体,并验证其在NIH 3T3细胞中的表达,为血管再狭窄的防治提供一个具有示踪和治疗双重作用的有效载体。方法以含有全长cDNA的pIRES-TFPI为模板,多聚... 详细信息

目的构建含人组织因子途径抑制因子(TFPI)和绿色荧光蛋白(GFP)基因的双顺反子真核表达载体,并验证其在NIH 3T3细胞中的表达,为血管再狭窄的防治提供一个具有示踪和治疗双重作用的有效载体。方法以含有全长cDNA的pIRES-TFPI为模板,多聚酶链反应(PCR)扩增TFPI全长cDNA,经酶切后插入到pIRES2-AcGFP1-Nuc载体中,构建成双顺反子真核表达载体,重组质粒经酶切图谱分析、PCR扩增及测序鉴定后命名为pIRES2-AcGFP1-Nuc-TFPI。将其转染NIH3T3细胞,采用荧光显微镜观察GFP在细胞中的表达,以RT-PCR检测TFPI在细胞内的表达。结果经酶切图谱分析、PCR扩增及DNA测序证实双顺反子真核表达载体构建正确;荧光显微镜可观察到细胞内GFP的表达;RT-PCR证实经TFPI基因转染的细胞内TFPI mRNA表达增高。结论成功构建了包括TFPI和GFP的真核双表达载体,并使其在NIH3T3细胞中顺利表达。

关键词：组织因子途径抑制因子绿色荧光蛋白双顺反子表达载体血管再狭窄

Ordered, Extra-large Mesoporous Tungstated Zirconia with Ultrastable Strong Brnsted Acid Sites

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Ordered, Extra-large Mesoporous Tungstated Zirconia with Ult...

全膝关节置换术中内侧副韧带股骨止点撕脱骨折单纯缝合7例

第十四届全国青年催化学术会议

作者： Changlei Chen sha Li shaodan Xu Liping Xiao Hui lou Jie Fan Key Lab of Applied Chemistry of Zhejiang Province Department of Chemistry Zhejiang University

＜正＞The long-term stability of a catalyst is a critical issue for their industrial applications operated at elevated temperature 1 . In order to simulate the long-term operation, thermally aging at extremely high te...

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临床骨科杂志 2024年第5期27卷 719-719页

作者：张威吴厦高宏娄佳旺刘璞蔡谞北京清华长庚医院骨科北京市红十字会和平骨科医院关节脊柱科北京美德瑞骨科医生集团

2016年9月～2021年4月，在北京清华长庚医院行初次全膝关节置换术的患者中，7例发生内侧副韧带股骨止点撕脱骨折。7例术中均使用W4843线反复“8”字缝合2～4次固定骨折块，缝合后测试骨折块稳定性好、无移位。术后患者佩带铰链式膝关节... 详细信息

2016年9月～2021年4月，在北京清华长庚医院行初次全膝关节置换术的患者中，7例发生内侧副韧带股骨止点撕脱骨折。7例术中均使用W4843线反复“8”字缝合2～4次固定骨折块，缝合后测试骨折块稳定性好、无移位。术后患者佩带铰链式膝关节支具，4周后可在带支具下进行完全负重锻炼。患者均获得6个月随访。术后1个月，1例复查X线片显示骨折块移位，但关节稳定性良好，其余患者未见骨折移位情况;7例伸直位行外翻应力试验均未见韧带松弛，1例屈膝30°位出现韧带轻度松弛。末次随访时，7例患者KSS评分由术前的63～137(98.4±20.6)分提高至145～189(164.9±17.7)分，膝关节屈曲活动度由术前的65°～95°(76.3°±11.0°)提高至112°～125°(118.5°±3.8°);所有患者对手术效果满意，膝关节功能恢复良好。

关键词：全膝关节置换术内侧副韧带撕脱骨折

A genetically engineered Escherichia coli that senses and degrades tetracycline antibiotic residue

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Synthetic and Systems Biotechnology 2018年第3期3卷 196-203页

作者： Zepeng Mu Zhuoning Zou Ye Yang Wenbo Wang Yue Xu Jianyi Huang Ruiling Cai Ye Liu Yajin Mo Boyi Wang Yiqun Dang Yongming Li Yushan Liu Yueren Jiang Qingyang Tan Xiaohong Liu Cheng Hu Hua Li sha Wei Chunbo lou Yang Yu Jiangyun Wang University of Chinese Academy of Sciences Team for iGEM 2016 Beijing100049China Key Laboratory of RNA Biology Institute of BiophysicsChinese Academy of SciencesBeijing100101China National Laboratory of Biomacromolecules CAS Center for BiomacromoleculesBeijing100101China Key Laboratory of Microbial Physiological and Metabolic Engineering Institute of MicrobiologyChinese Academy of SciencesBeijing100101China College of Life Sciences University of Chinese Academy of SciencesBeijing100049China Tianjin Institute of Industrial Biotechnology Chinese Academy of SciencesTianjin300308China

Due to the abuse of antibiotics,antibiotic residues can be detected in both natural environment and various industrial products,posing threat to the environment and human *** we describe the design and implementation of an engineered Escherichia coli capable of degrading tetracycline(Tc)-one of the commonly used antibiotics once on humans and now on poultry,cattle and fisheries.A Tcdegrading enzyme,TetX,from the obligate anaerobe Bacteroides fragilis was cloned and recombinantly expressed in *** and fully characterized,including its Km and kcat *** quantitatively evaluated its activity both in vitro and in vivo by UVeVis spectrometer and ***,we used a tetracycline inducible amplification circuit including T7 RNA polymerase and its specific promoter PT7 to enhance the expression level of TetX,and studied the dose-response of TetX under different inducer *** the deployment of genetically modified organisms(GMOs)outside laboratory brings about safety concerns,it is necessary to explore the possibility of integrating a ***-Antitoxin(TA)systems were used to construct a mutually dependent host-plasmid platform and biocontainment systems in various academic and industrious *** selected nine TA systems from various bacteria strains and measured the toxicity of toxins(T)and the detoxifying activity of cognate antitoxins(A)to validate their potential to be used to build a *** results prove the possibility of using engineered microorganisms to tackle antibiotic residues in environment efficiently and safely.

关键词： TetX Antibiotic residue Toxin-antitoxin system Kill-switch

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医用植入物材料与宿主相互作用及生物安全性

中国组织工程研究与临床康复 2008年第49期12卷 9755-9758页

作者：王莉莎罗艳丽 <中国组织工程研究与临床康复>杂志社辽宁省沈阳市110004

医用植入物材料是指种植、埋藏、固定于宿主受损或病变部位,支持、修复、替代其功能的一类特殊的医用消耗性材料,是一类具有特殊性能和特种功能,用于人工器官再造、外科修复、理疗康复、疾病诊断及治疗,对人体组织、血液无不良影响的材... 详细信息

医用植入物材料是指种植、埋藏、固定于宿主受损或病变部位,支持、修复、替代其功能的一类特殊的医用消耗性材料,是一类具有特殊性能和特种功能,用于人工器官再造、外科修复、理疗康复、疾病诊断及治疗,对人体组织、血液无不良影响的材料。由于医用植入物大多需植入人体而发挥作用,因此,对医用植入物材料的安全性具有较高的要求,其临床使用规范和相关安全性评价标准也一直在不断提高和不断完善中。

关键词：生物材料医用植入物材料骨科植入物人工器官生物相容性生物安全性

基于自相关特性的经验模态分解微机械陀螺去噪方法

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中国惯性技术学报 2016年第4期24卷 537-541页

作者：刘晓光郝沙沙王光磊娄存广刘秀玲河北大学电子信息工程学院保定071002 河北省数字医疗工程重点实验室保定071002

陀螺的噪声是影响组合导航系统精度的重要因素之一。以农机多传感器组合导航系统为研究背景,在分析经验模态分解去噪和小波去噪的基础上,提出了一种基于自相关特性的经验模态分解去噪方法。该方法根据本证模态函数分量的自相关函数特性... 详细信息

陀螺的噪声是影响组合导航系统精度的重要因素之一。以农机多传感器组合导航系统为研究背景,在分析经验模态分解去噪和小波去噪的基础上,提出了一种基于自相关特性的经验模态分解去噪方法。该方法根据本证模态函数分量的自相关函数特性,提出了一种含噪本证模态函数筛选策略。该方法能够自适应地确定主要含噪的本证模态函数分量,避免了需要人为确定的不足;同时,结合改进小波阈值去噪的优势,避免了将混叠在噪声中的有效信号完全消除,使其具有一定的自适应性。为了验证方法的有效性,利用农机组合导航系统中微机械陀螺的实际输出数据,分别采用改进阈值小波去噪方法、经验模态分解去噪和改进的经验模态分解去噪方法进行了对比试验。结果表明,改进经验模态分解去噪方法的效果要优于前者,在一定程度上能够改善农机多传感器组合导航系统的定位精度。

关键词：经验模态分解微机械陀螺去噪数据处理