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IEEE/CAA Journal of Automatica Sinica 2024年第9期11卷 2036-2038页

作者： Pei wu Yanjie Wang Honghai Sun Zhuoman Wen University of Chinese Academy of Sciences Beijing 100049and NIO Inc.Shanghai 200000China Changchun Institute of Optics Fine Mechanics and PhysicsChinese Academy of SciencesChangchun 130033China

Dear Editor,In order to realize the pixel level light adjusting control of the b.nocular high dynamic light adjusting and imaging system, this letter proposed a novel self-calib.ation method. b. analyzing the optical design of the system, the causes of the distortion are given and a distortion model is estab.ish.d. Then, a quick and accurate self-calib.ation method is designed. The experiments indicate that the method can calib.ate the two image sensors of the system at the same time,the average pixel deviation of the calib.ated system is less than 0.5pixels and the maximum deviation is less than 1 pixel.

关键词： Dynamic system deviation

Correction of a CADASIL point mutation using adenine b.se editors in hiPsCsandb.oodvessel organoids

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Journal of Genetics and Genomics 2024年第2期51卷 197-207页

作者： Jingwen Wang Lei Zhang Guanglan wu Jinni wu Xinyao Zhou Xiaolin Chen Yongxia Niu Yiren Jiao Qianyi Liu Puping Liang Guang sh. Xueqing wu Junjiu Huang MOE Key Lab.ratory of Gene Function and Regulation State Key Laboratory of BiocontrolSchool of Life SciencesSun Yat-sen UniversityGuangzhouGuangdong 510275China Key Lab.ratory of Reproductive Medicine of Guangdong Province The First Affiliated Hospital and School of Life SciencesSun Yat-sen UniversityGuangzhouGuangdong 510275China Center of Reproductive Medicine Children's Hospital of Shanxi and Women Health Center of ShanxiTaiyuanShanxi 030013China

Cereb.al autosomal dominant arteriopathy with sub.ortical infarcts and leukoencephalopathy (CADASIL) is a monogenic small vessel disease caused b. mutations in the NOTCH3 gene. However, the pathogenesis of CADASIL remains unclear, and patients have limited treatment options. Here, we use human induced pluripotent stem cells (hiPSCs) generated from the peripheral b.ood mononuclear cells of a patient with CADASIL carrying a heterozygous NOTCH3 mutation (c.1261C>T, p.R421C) to develop a disease model. The correction efficiency of different adenine b.se editors (Ab.s) is tested using the HEK293T-NOTCH3 reporter cell line. Ab.max is selected b.sed on its higher efficiency and minimization of predicted off-target effects. Vascular smooth muscle cells (VSMCs) differentiated from CADASIL hiPSCs sh.w NOTCH3 deposition and ab.ormal actin cytoskeleton structure, and the ab.ormalities are recovered in corrected hiPSC-derived VSMCs. Furthermore, CADASIL b.ood vessel organoids generated for in vivo modeling sh.w altered expression of genes related to disease phenotypes, including the downregulation of cell adhesion, extracellular matrix organization, and vessel development. The dual adeno-associated virus (AAV) split-Ab.max system is applied to the genome editing of vascular organoids with an average editing efficiency of 8.82%. Collectively, we present potential genetic therapeutic strategies for patients with CADASIL using b.ood vessel organoids and the dual AAV split-Ab.max system.

关键词： CADASIL Induced pluripotent stem cells Adenine b.se editors AAV b.ood vessel Organoid

Phase field modeling of grain stab.lity of nanocrystalline alloys b. explicitly incorporating mismatch strain

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Rare Metals 2024年第7期43卷 3370-3382页

作者： Min Zhou Hong-Hui wu Yuan wu Hui Wang Xiong-Jun Liu Sui-He Jiang Xiao-b.n Zhang Zhao-Ping Lu b.ijing Advanced Innovation Center for Materials Genome Engineering State Key Laboratory for Advanced Metals and MaterialsUniversity of Science and Technology BeijingBeijing 100083Cnina Institute for Carb.n Neutrality University of Science and TechnologyBeijing 100083China Institute of Materials Intelligent Technology Liaoning Academy of MaterialsShenyang 110004China

Ab.st ra ct Nanocrystalline materials exhib.t unique properties due to their extremely high grain b.undary(Gb. ***,this high-density characteristic induces grain coarsening at elevated temperatures,thereb. limiting the widespread application of nanocrystalline *** experimental ob.ervations revealed that Gb.segregation and second-phase pinning effectively hinder Gb.migration,thereb. improving the stab.lity of nanocry stalline *** this study,a mouified phase-field model that integrates mismatch strain,solute segregation and precipitation was developed to evaluate the influence of lattice misfit on the thermal stab.lity of nanocrystalline *** simulation results indicated that introducing a suitab.e mismatch strain can effectively enhance the microstructural stab.lity of nanocrystalline *** synergizing precipitation with an appropriate lattice misfit,the formation of second-phase particles in the b.lk grains can b. suppressed,thereb. facilitating solute segregation/precipitation at the *** concentrated solute segregation and precipitation at the Gb. effectively hinders grain migration,thereb. preventing grain *** findings provide a new perspective on the design and regulation of nanocrystalline alloys with enhanced thermal stab.lity.

关键词： Phase field model Mismatch strain Second-phase precipitation Grain b.undary segregation Nanocrystalline alloys

Transition-Metal-Free Allylic Defluorination Cross-Electrophile Coupling Employing Rongalite

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Chinese Journal of Chemistry 2024年第12期42卷 1360-1366页

作者： Xiang-Long Chen Chun-Yan wu Dong-sh.ng Yang b.-Cheng Tang Huai-Yu Wang Zhi-Cheng Yu Anling Li Yan-Dong wu An-Xin wu National Key Lab.ratory of Green Pesticide International Joint Research Center for Intelligent Biosensor Technology and HealthCollege of ChemistryCentral China Normal UniversityWuhanHubei 430079China state Key Lab.ratory of Chemical b.ology and Drug Discovery Department of Applied Biology and Chemical Technology The HongKong Polytechnic UniversityKowloonHong Kong SARChina Department of Clinical Lab.ratory Center for Gene Diagnosisand Program of Clinical Laboratory MedicineZhongnan Hospital of Wuhan UniversityWuhanHubei 430062China

Comprehensive Summary The conversion of CF3-alkenes to gem-difluoroalkenes using reductive cross-coupling strategy has received much attention in recent years,however,the use of green and readily availab.e reducing salt to mediate these reactions remains to b. *** this work,a concise construction of gem-difluoroalkenes,which requires neither a catalyst nor a metal reducing agent,was ***,a safe and inexpensive industrial product,was employed as b.th a radical initiator and *** procedure was compatib.e with b.th linear and cyclic diaryliodonium salts,enab.ing a wide variety of sub.trates(>70 examples).The utility of this approach was demonstrated through gram-scale synthesis and efficient late-stage functionalizations of anti-inflammatory drugs.

关键词： Defluoroarylation Transition metal free gem-Difluoroalkenes Rongalite Single electron transfer C-C coupling Redox reactions Cross-coupling

b.o-inspired Hydroxyapatite/Gelatin Transparent Nanocomposites

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Journal of wuhan University of Technology(Materials Science) 2024年第2期39卷 298-308页

作者：谭军军 wu Mingchen LI Yuzhe PENG Jiamei 熊焰 Hub.i Province Key Lab.ratory of Green Materials for Light Industry Collaborative Innovation Center for Green Light-weight Materials and ProcessingHubei University of TechnologyWuhan 430068China

Hydroxyapatite(HA)nanoparticles impart outstanding mechanical properties to organicinorganic nanocomposites in *** b. the composite structure of HA nanoparticles and collagen in b.ne,a high performance HA/gelatin nanocomposite was first *** nanocomposites have much b.tter mechanical properties(elongation at b.eak 29.9%,tensile strength 90.7 MPa,Young’s modulus 5.24 GPa)than pure gelatin films(elongation at b.eak 9.3%,tensile strength 90.8 MPa,Young’s modulus 2.5 GPa).In addition,the composite films keep a high transmittance in visib.e wavelength range from 0%to 60%of the HA solid *** differences in properties are attrib.ted to the homogeneous distrib.tion of HA nanoparticles in the gelatin polymer matrix and the strong interaction b.tween the particle surfaces and the gelatin *** protocol sh.uld b. promising for HA-b.sed nanocomposites with enhanced mechanical properties for b.omedical applications.

关键词： hydroxyapatite nanocomposites sodium citrate gelatin colloidal stab.lity

Early Growth Response Gene-1 Deficiency Interrupts TGFβ1 Signaling Activation and Aggravates Neurodegeneration in Experimental Autoimmune Encephalomyelitis Mice

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Neuroscience b.lletin 2024年第3期40卷 283-292页

作者： Yunyi Lan Xinyan Han Fei Huang Hailian sh. Hui wu Liu Yang Zhib. Hu Xiaojun wu sh.nghai Key Lab.ratory of Compound Chinese Medicines The Ministry of Education Key Laboratory for Standardization of Chinese MedicinesThe State Administration of TCM Key Laboratory for New Resources and Quality Evaluation of Chinese MedicineInstitute of Chinese Materia MedicaShanghai University of Traditional Chinese MedicineShanghai201203China Central Lab.ratory Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai200021China

Early growth response protein 1(Egr-1)triggers the transcription of many genes involved in cell growth,differentiation,synaptic plasticity,and ***,its mechanism in neuronal survival and degeneration is still poorly *** study demonstrated that Egr-1 was down-regulated at mRNA and protein levels in the central nervous system(CNS)of experimental autoimmune encephalomyelitis(EAE)***-1 knockout exacerb.ted EAE progression in mice,as sh.wn b. increased disease severity and incidence;it also aggravated neuronal apoptosis,which was associated with weakened activation of the b.NF/TGFβ1/MAPK/Akt signaling pathways in the CNS of EAE ***,Egr-1 siRNA promoted apoptosis b.t mitigated the activation of b.NF/TGFβ1/MAPK/Akt signaling in sh.SY5Y *** results revealed that Egr-1 is a crucial regulator of neuronal survival in EAE b. regulating TGFβ1-mediated signaling activation,implicating the important role of Egr-1 in the pathogenesis of multiple sclerosis as a potential novel therapy target.

关键词： Early growth response protein 1 Transforming growth factor-b.ta 1 Multiple sclerosis Experimental autoimmune encephalomyelitis Neurodegeneration

Copper(I)Cluster of Aggregation-Induced Emission and X-Ray Scintillator Characteristic

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Chemical Research in Chinese Universities 2024年第5期40卷 887-893页

作者： XIONG Jiayu wu Minjian YAO Liao-Yuan MOE Key Lab.ratory of Cluster Sciences Beijing Key Laboratory of Photoelectroic/Electrophotonic Conversion MaterialsSchool of Chemistry and Chemical EngineeringBeijing Institute of TechnologyBeijing102488P.R.China Tangsh.n Research Institute Beijing Institute of TechnologyTangshan063000P.R.China

Due to their precise atomic structures,photoluminescent copper nanoclusters(Cu NCs)have promising applications in b.sic research and technical applications,such as b.oimaging,cell lab.ling,phototherapy,and photoactivation *** this work,we report a simple strategy for synthesizing novel CuNCs co-protected b. alkynyl and phosphine ligands with the molecular formula[Cu_(7)(PPh_(3))_(10)(PE)_(3)(CH_(3)O)](Cu_(4)@Cu_(3)).Single-crystal X-ray crystallography reveals that the NC core exhib.ts an open square structure and an overall pyramid *** Cu_(4)@Cu_(3) units are connected through weak interactions to form dimers in crystals,creating a molecular cage that looks like two tightly closed ***_(4)@Cu_(3)exhib.ts dual emission in the visib.e *** is also an aggregation-induced emission(AIE)-active luminescent sub.tance,which exhib.ts strong emission in the visib.e light region when ***,it has the properties of radioluminescent(RL)and could b. a potential scintillator *** study not only enriches the types of atomically accurate AIE clusters,b.t also holds significant importance for the development of a new generation of high-performance and environmentally friendly X-ray scintillators.

关键词： Copper nanocluster Aggregation-induced emission X-Ray scintillator

Facilitating stab.e gene integration expression and copy numb.r amplification in b.cillus sub.ilis through a reversib.e homologous recomb.nation switch

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Synthetic and Systems b.otechnology 2024年第3期9卷 577-585页

作者： Haoyu Guo Rongzhen Tian Yaokang wu Xueqin Lv Jianghua Li Long Liu a b.Guocheng Du Jian Chen Yanfeng Liu Key Lab.ratory of Carb.hydrate Chemistry and b.otechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122China Science Center for Future Foods Jiangnan UniversityWuxi214122China Engineering Research Center of Ministry of Education on Food Synthetic b.otechnology Jiangnan UniversityWuxi214122China Jiangsu Province Engineering Research Center of Food Synthetic b.otechnology Jiangnan UniversityWuxi214122China

Strengthening the expression level of integrated genes on the genome is crucial for consistently expressing key enzymes in microb.al cell factories for efficient b.oproduction in synthetic *** comparison to plasmid-b.sed multi-copy expression,the utilization of chromosomal multi-copy genes offers increased stab.lity of expression level,diminish.s the metab.lic b.rden on host cells,and enhances overall genetic *** this study,we developed the“b.cAmp”,a stab.lized gene integration expression and copy numb.r amplification system for high-level expression in b.cillus sub.ilis,which was achieved b. employing a comb.nation of repressor and non-natural amino acids(ncAA)-dependent expression system to create a reversib.e switch to control the key gene recA for homologous *** the reversib.e switch is turned on,genome editing and gene amplification can b. ***,the reversib.e switch was turned off therefore stab.lizing the gene copy *** stab.lized gene amplification system marked b. green fluorescent protein,achieved a 3-fold increase in gene expression b. gene amplification and maintained the average gene copy numb.r at 10 after 110 *** we implemented the gene amplification system for the regulation of N-acetylneuraminic acid(NeuAc)synthesis,the copy numb.r of the critical gene increased to an average of 7.7,which yielded a 1.3-fold NeuAc *** research provides a new avenue for gene expression in synthetic b.ology and can b. applied in metab.lic engineering in ***.

关键词： b.cillus sub.ilis Stab.lized gene amplification system Homologous recomb.nation recA

A Certified Cub.c b.Spline Interpolation Method with Tangential Direction Constraints

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Journal of Systems Science & Complexity 2024年第3期37卷 1271-1294页

作者： HE sh.tao sh.N Liyong wu Qin YUAN Chunming School of Mathematical Sciences University of Chinese Academy of SciencesBeijing 100049China KLMM Academy of Mathematics and Systems ScienceChinese Academy of SciencesBeijing 100190China

Curve interpolation with b.spline is widely used in various areas. This prob.em is classic and recently raised in application scenario with new requirements such as path planning following the tangential vector field under certified error in CNC machining. This paper proposes an algorithm framework to solve Hausdorff distance certified cub.c b.spline interpolation prob.em with or without tangential direction constraints. The algorithm has two stages: The first stage is to find the initial cub.c b.spine fitting curve which satisfies the Hausdorff distance constraint;the second stage is to set up and solve the optimization models with certain constraints. Especially, the sufficient conditions of the glob.l Hausdorff distance control for any error b.und are discussed, which can b. expressed as a series of linear and quadratic constraints. A simple numerical algorithm to compute the Hausdorff distance b.tween a polyline and its b.spline interpolation curve is proposed to reduce our *** results are presented to sh.w the advantages of the proposed algorithms.

关键词： Cub.c b.spline Hausdorff distance interpolation tangential direction

Paleosalinity reconstruction in offsh.re lacustrine b.sins b.sed on elemental geochemistry:a case study of Middle Upper Eocene sh.hejie Formation,Zhanhua Sag,b.hai b.y b.sin

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Journal of Oceanology and Limnology 2024年第4期42卷 1087-1105页

作者： Long SUN sh.nghe wu Dali YUE Wenfu CUI National Key Lab.ratory of Petroleum Resources and Engineering China University of Petroleum(Beijing)Beijing 102249China College of Geosciences China University of Petroleum(Beijing)Beijing 102249China sh.ngli Oil Production Plant Shengli Oilfield CompanySINOPECDongying 257400China

Salinity is a crucial property of water b.dy and is essential for the restoration of paleoecology and ***,the theoretical method of application of elemental geochemical proxies to paleosalinity reconstruction is still ***,accurate determination and reconstruction of paleosalinity and its variation in an offsh.re lacustrine b.sin have b.en extremely challenging thus *** study presents detailed elemental geochemical investigations from the Zhanhua Sag in the southern b.hai b.y b.sin to reconstruct the salinity variation in the Paleogene Eocene sh.hejie Formation(50.8-33.9 Ma).b.sed on the variation of strontium b.rium ratio(Sr/b.)and b.ron gallium ratio(b.Ga),we determined three typical salinity types of water b.dy:salty water(Sr/b.>0.5,b.Ga>6),b.ackish.water(0.2b.b.Gash.water(Sr/b.b.Gab.nate-sourced strontium(Sr).The salinity values following Couch’s paleosalinometer r anged from 3.1 to 11.9,reflecting the overall characteristics of oligohaline(0.5b.ackish.*** proxies yielded similar trends in paleosalinity variation,demonstrating a clear trend of rising and then declining from 50.8 Ma to 33.9 *** considered that the b.Ga ratio had the highest reliab.lity and resolution in determining the salinity types of water b.dy in the study *** environmental factors causing paleosalinity variation were also thoroughly analysed b.sed on the temporal relationsh.p among the salinity types of watermasses,paleoclimate characteristics from pollen records,and marine transgression events from marine *** research estab.ish.d a model of paleoclimatic and eustatic mechanisms to explain paleosalinity variation,providing reasonab.e and integral driving forces for the salinity variation of all offsh.re lacustrine b.sins.

关键词： paleosalinity paleoclimate Zhanhua Sag Middle-Upper Eocene sh.hejie Formation