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Transporting Mammalian Cells at Ambient Temperature:A Viable Alternative to Dry Ice

Advances in Bioscience and Biotechnology 2017年第4期8卷 127-133页

作者： Steingrimur Stefansson seunghyun han Ye In Jeon David S.Chung Peter Hwang Huyen Le John L.Warden David Ho HeMemics Biotechnologies Inc RockvilleUSA Johns Hopkins University BaltimoreUSA George Washington University WashingtonDCUSA Division of Biology and Medicine Brown UniversityProvidenceUSA Thomas Jefferson High School for Science and Technology AlexandriaUSA Nauah Solutions McleanUSA

The most common method of shipping cells between institutes and companies is sending them frozen, usually treated with anti-freeze solution (most commonly DMSO because it is less toxic than many alternatives), and then packaging them in dry ice for shipment. However many countries place restrictions on dry ice shipments. An alternative to shipping frozen cell vials is to send flasks of growing cells in media. This also has problems because cells in media have limited viability and the flasks can leak. Here we report on an alternative method for shipping viable cells at ambient temperature without dry ice or in media filled flasks. In this study we report on the development and properties of HemSol?. This is an inexpensive, eco-friendly and protects cell integrity at ambient temperature while maintaining viability. We have previously shown that HemSol? protects platelet and RBC function in cold storage and circulating tumor cells up to 6 days. Therefore we wanted to know if HemSol? could also be used to transport live cells. Since HemSol? is a liquid, we experimented with encasing the cells with HemSol? and gelatin so as to prevent dry ice shipment of cells and circumvent the shipping of cells in media. We performed mock shipping experiments where cells were stored in HemSol? gel kept at room temperature on a lab benchtop and cells stored in dry ice was also kept on lab benchtop for up to 2 days. After the mock shipping period, we analyzed cells for their functions. Our results show that cells in HemSol? gel have greater than 95% viability and restored biological functions in 2 hours, whereas, cells shipped in dry ice required more than 24 hours to recover and needed media change to remove the DMSO.

关键词： HemSol^(TM) HemSol^(TM) Gel Transportation Short Term Storage Cells Shipment Dry Ice Ambient Temperature Shipment

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Germinant ZnO nanorods as a charge-selective layer in organic solar cells

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Journal of Materials Science & Technology 2020年第20期52卷 89-94页

作者： Kunsik An Jaehoon Kim Mohammad Afsar Uddin seunghyun Rhee Hyeok Kim Kyung-Tae Kang han Young Woo Changhee Lee Department of Electrical and Computer Engineering Seoul National UniversitySeoul 08826Republic of Korea Micro/Nano Process Group Korea Institute of Industrial Technology(KITECH)Sangnok-guAnsan-si 15588Republic of Korea Department of Chemistry College of ScienceKorea UniversitySeoul 02841Republic of Korea Department of Electrical Engineering Engineering Research Institute(ERI)Gyeongsang National UniversityJinju 52828Republic of Korea

A facile method was introduced and demonstrated to synthesize zinc oxide(ZnO) nanorods(NRs) as an electron transporting layer(ETL) for organic solar cells(OSCs).Hydrothermal synthesis of the NRs showed a constant growth rate of 5.5 nm min-1 from germination to sub-micrometer *** properties were characterized using scanning electron microscopy(SEM),transmission electron microscopy(TEM),absorption spectrophotometry and so *** on these measurements,the germinant growth mechanism and its corresponding orientation characteristics were *** an ETL of the OSCs,ZnO NRs enhance the charge extraction from the active layer due to their increased interfacial surface area,but there is an optimal length because of the shunt path formation and UV absorption of long ZnO *** a re sult,the OSC with the ZnO NRs as ETL shows power conversion efficiency(PCE) up to 6.2%.The J-V characteristics and incident photon-to-current conversion efficiency(IPCE) measurement also reveal that the efficiency enhancement is an assembly of individual results from optical,physical and electrical characteristic of the ZnO NRs.

关键词： ZnO nanorods Solar cells Self-assembly Oriented growth mechanism Hydrothermal synthesis

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The influence of environmental factors on species composition and distribution in a community forest in Northern Thailand

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Journal of Forestry Research 2021年第2期32卷 649-662页

作者： Siriluck Thammanu Dokrak Marod Hee han Narayan Bhusal Lamthai Asanok Pipat Ketdee Noppakoon Gaewsingha seunghyun Lee Joosang Chung Department of Forest Sciences Seoul National UniversitySeoul 08826Republic of Korea Royal Forest Department Bangkok 10900Thailand Department of Forest Biology Faculty of ForestryKasetsart UniversityBangkok 10900Thailand Department of Forest Policy and Economics National Institute of Forest ScienceSeoul 02455Republic of Korea Department of Agroforestry Maejo UniversityPhrae CampusPhrae 54140Thailand Department of National Parks Wildlife and Plant ConservationBangkok 10900Thailand Research Institute of Agriculture and Life Sciences Seoul National UniversitySeoul 08826Republic of Korea

Understanding the environmental factors that influence tree species composition is essential for successful management of biodiversity and sustainable use of community forest *** study aims to assess tree species composition and distribution in the deciduous Ban Mae Chiang Rai Lum Community Forest in Northern Thailand and to analyze the influence of environmental factors on tree biodiversity in the *** conducted a stratified systematic sampling of the forest’s total area of 3925 ha,and twenty-five 0.16 ha survey plots were established in three different stands of the deciduous forests to estimate and characterize the difference in biological diversity among the *** correspondence analysis(CCA)was used to investigate the environment factors affecting such differences in biodiversity of the *** results showed a high diversity of trees in the forest as 197 species,144 genera,and 62 plant families were *** CCA ordination identified the environmental factors—the most important of which were elevation,distance to streams,soil moisture,organic matter,and distance to communities—that signifi-cantly influenced the diversity and distribution of tree species(p<0.05)in the community *** findings indicate that the implementation of drought reduction measures such as building check dams,fire protection,and monitoring community forest-product usage would be recommended to further biodiversity conservation and the sustainable use of community forest resources.

关键词： Community forest management Species biodiversity Canonical correspondence analysis Ban Mae Chiang Rai Lum community forest Northern Thailand

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