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Novel monoclonal antibody against beta 1 integrin enhances cisplatin efficacy in human lung adenocarcinoma cells

The Journal of Biomedical Research 2016年第3期30卷 217-224页

作者： Min-Young Kim Woon-Dong Cho Kwon Pyo hong Da Bin Choi Jeong won hong Soseul Kim Yoo Ri Moon seung-Myoung Son Ok-Jun Lee Ho-Chang Lee Hyung Geun Song Department of Pathology Chungbuk National University College of MedicineCheongju28644Republic of Korea Research Institute Chungbuk National University College of MedicineCheongju28644Republic of Korea

The use of anti-beta 1 integrin monoclonal antibody in lung cancer treatment has proven beneficial. Here, we developed a novel monoclonal antibody （mAb）, called P5, by immunizing mice with human peripheral blood mononuclear cells （PBMC）. Its anti-tumor effect is now being tested, in a clinical phase Ⅲ trial, in combinato- rial treatments with various chemical drugs. To confirm that P5 indeed binds to beta 1 integrin, cell lysates were immunoprecipitated with commercial anti-beta 1 integrin mAb （TS2/16） and immunoblotted against P5 to reveal a 140 kDa molecular weight band, as expected. Immunoprecipitation with P5 followed by LC/MS protein sequence analysis further verified P5 antigen to be beta 1 integrin. Cisplatin treatment upregulated cell surface expression of beta 1 integrin in A549 cells, while causing inhibition of cell growth. When cells were co-treated with different concentrations of P5 mAb, the cisplatin-mediated inhibitory effect was enhanced in a dose-dependent manner. Our findings show that a combinatorial treatment of P5 mAb and cisplatin in A549 cells resulted in a 30% increase in apoptosis, compared to baseline, and significantly more when compared to either the cisplatin or P5 alone group. The entire peptide sequences in CDR from variable region of Ig heavy and light chain gene for P5 mAb are also disclosed. Together, these results provide evidence of the beneficial effect of P5 mAb in combinatorial treatment of human lung adenocarcinoma.

关键词： CD29 beta 1 integrin lung adenocarcinoma monoclonal antibody cisplatin

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Vertical graphene on flexible substrate,overcoming limits of crack-based resistive strain sensors

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npj Flexible Electronics 2022年第1期6卷 28-35页

作者： hong Ryeol Na Hyun Joo Lee Jae Ho Jeon Han-Jin Kim Sahng-Kyoon Jerng Sanjib Baran Roy seung-Hyun Chun Sunghun Lee Yong Ju Yun Department of Physics and Astronomy Sejong UniversitySeoul 05006Korea Graduate School of Energy and Environment(KU-KIST Green School) Korea UniversitySeoul 02841Korea Center for Energy Materials Research Korea Institute of Science and TechnologySeoul 02792Korea Graphene Research Institute Sejong UniversitySeoul 05006Korea

Resistive strain sensors(RSS)with ultrasensitivity have attracted much attention as multifunctional ***,since most ultrasensitive RSS are designed by cracked conductive metals,the sensing performance is severely degraded due to accumulated structural deformation with consecutive *** overcome such limitation,newly designed structures have been suggested,but the development of mechanosensors exhibiting superior stability and ultrasensitivity still remains a ***,we demonstrate that vertical graphene(VG)RSS with high sensitivity(gauge factor greater than 5000),remarkable durability(>10,000 cycles),and extraordinary resilience can serve multifunctional *** find that well-defined cracks on tufted network structure result in highly reversible resistance variation,especially revivable status even after broken current path,confirmed by microscopic in situ *** VG integrated with a wireless sensing system exhibits excellent timbre recognition *** findings provide inspirable insights for mechanosensing system,making VG a promising component for future practicable flexible sensor technologies.

关键词： strain durability Vertical

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Surgical outcome of pancreatic cancer using radical antegrade modular pancreatosplenectomy procedure

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World Journal of Gastroenterology 2012年第39期18卷 5595-5600页

作者： Ye Rim Chang Sung-Sik Han Sang-Jae Park seung Duk Lee Tae Suk Yoo Young-Kyu Kim Tae Hyun Kim Sang Myung Woo Woo Jin Lee Eun Kyung hong Department of Surgery Seoul National University HospitalSeoul 110-744South Korea Center for Liver Cancer National Cancer CenterGyeonggido 411-764South Korea

AIM:To evaluate the surgical outcomes following radical antegrade modular pancreatosplenectomy (RAMPS) for pancreatic cancer. METHODS:Twenty-four patients underwent RAMPS with curative intent between January 2005 and June 2009 at the National Cancer Center, South Korea. Clinicopathologic data, including age, sex, operative findings, pathologic results, adjuvant therapy, postop-erative clinical course and follow-up data were retro-spectively collected and analyzed for this study. RESULTS:Twenty-one patients (87.5%) underwent distal pancreatectomy and 3 patients (12.5%) underwent total pancreatectomy using RAMPS. Nine patients (37.5%) underwent combined vessel resection, including 8 superior mesenteric-portal vein resections and 1 celiac axis resection. Two patients (8.3%) underwent combined resection of other organs, including the colon, stomach or duodenum. Negative tangential margins were achieved in 22 patients (91.7%). The mean tumor diameter for all patients was 4.09 ± 2.15 cm. The 2 patients with positive margins had a mean diameter of 7.25 cm. The mean number of retrieved lymph nodes was 20.92 ± 11.24 and the node positivity rate was 70.8%. The median survival of the 24 patients was 18.23 ± 6.02 mo. Patients with negative margins had a median survival of 21.80 ± 5.30 mo and those with positive margins had a median survival of 6.47 mo (P = 0.021). Nine patients (37.5%) had postoperative complications, but there were no postoperative mortalities. Pancreatic fistula occurred in 4 patients (16.7%):2 patients had a grade A fistula and 2 had a grade B fistula. On univariate analysis, histologic grade, positive tangential margin, pancreatic fistula and adjuvant therapy were significant prognostic factors for survival. CONCLUSION:RAMPS is a feasible procedure for achieving negative tangential margins in patients with carcinoma of the body and tail of the pancreas.

关键词： Carcinoma Pancreas Surgical resection Sur-vival Radical antegrade modular pancreatosplenectomy

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基于Bayes估计的WSN自适应路由算法

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计算机工程 2010年第21期36卷 86-88页

作者：李晓卉方康玲 hong seung Ho 武汉科技大学冶金自动化与检测技术教育部工程研究中心武汉430081 Department of Electronics Information and System EngineeringHanyang University

针对无线传感器网络中存在的路由请求域动态调整问题,提出一种基于Bayes估计的无线传感器网络自适应路由算法。该算法以圆柱形路由请求域的半径作为调整参数,利用Bayes估计对路由请求域进行自适应调整。仿真结果表明,该算法在数据包投... 详细信息

针对无线传感器网络中存在的路由请求域动态调整问题,提出一种基于Bayes估计的无线传感器网络自适应路由算法。该算法以圆柱形路由请求域的半径作为调整参数,利用Bayes估计对路由请求域进行自适应调整。仿真结果表明,该算法在数据包投递率、路由开销、数据包平均时延和网络剩余能量比率上的性能都优于AODVjr路由算法。

关键词：无线传感器网络路由算法 Bayes估计

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Effects of feeding a diet containing Gymnema sylvestre extract: attenuating progression of obesity in C57BL/6J mice

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Asian Pacific Journal of Tropical Medicine 2016年第5期9卷 425-431页

作者： Hyeon-Jeong Kim Seong-Ho hong seung-Hee Chang Sanghwa Kim Ah Young Lee Yoonjeong Jang Orkhonselenge Davaadamdin Kyeong-Nam Yu Ji-Eun Kim Myung-Haing Cho Laboratory of Toxicology College of Veterinary Medicine Seoul National University Graduate Group of Tumor Biology Seoul National University Graduate School of Convergence Science and Technology Seoul National University Advanced Institutes of Convergence Technology Seoul National University Institute of Green Bio Science Technology Seoul National University

Objective: To investigate the effect of Gymnema sylvestre extract(GS) on initial anti-obesity, liver injury, and glucose homeostasis induced by a high-fat diet(HFD). Methods: The dry powder of GS was extracted with methanol, and gymnemic acid was identified by high performance liquid chromatography as deacyl gymnemic acid. Male C57BL/6J mice that fed on either a normal diet, normal diet containing 1 g/kg GS(CON+GS) HFD, or HFD containing 1.0 g/kg GS(HFD+GS) for 4 weeks were used to test the initial anti-obesity effect of GS. Body weight gain and food intake, and serum levels about lipid and liver injury markers were measured. Histopathology of adipose tissue and liver stained with hematoxylin and eosin(H&E) and oil-red O were analyzed. After 4 weeks of GS extract feeding, intraperitoneal glucose tolerance test(IPGTT) was performed. Results: The methanol extracts of GS exerted significant anti-obesity effects in HFD+GS group. They decreased body weight gain, a lower food and energy efficiency ratio, and showed lower serum levels of total cholesterol(TC), triglyceride(TG), low-density lipoprotein(LDL)-cholesterol, very-low density lipoprotein(VLDL)-cholesterol and leptin compared with the HFD group. The decreases of abdominal as well as epididymal fat weight and adipocyte hypertrophy, lipid droplets in liver, and serum levels of aspartate aminotransferase(AST) and alanine transaminase(ALT) were also observed. The CON+GS group showed an effect of glucose homeostasis compared to the CON group. Conclusions: This study shows that GS provide the possibility as a key role in an initial anti-obesity effects feeding with a HFD.

关键词： Gymnema sylvestre extract Obesity Adipocyte Body weight gain High-fat diet Liver injury

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Clinical assessment and identification of immuno-oncology markers concerning the 19-gene based risk classifier in stage Ⅳ colorectal cancer

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World Journal of Gastroenterology 2019年第11期25卷 1341-1354页

作者： Jong Lyul Lee Seon Ae Roh Chan wook Kim Yi hong Kwon Ye Jin Ha Seon-Kyu Kim Seon-Young Kim Dong-Hyung Cho Yong Sung Kim Jin Cheon Kim Department of Surgery University of Ulsan College of Medicine Institute of Innovative Cancer Research Asan Medical Center Medical Genomics Research Center Korea Research Institute of Bioscience and Biotechnology School of Life Sciences Kyungpook National University

BACKGROUND Genomic profiling of tumors has contributed to the understanding of colorectal cancer(CRC), facilitating diagnosis, prognosis and selection of treatments,including targeted regimens. A report suggested that a 19-gene-based risk classifier(TCA19) was a prognostic tool for patients with stage III CRC. The survival outcomes in patients with stage IV CRC are still poor and appropriate selection of targeted therapies and immunotherapies is *** To assess clinical implication of TCA19 in patients with stage IV CRC, and to identify TCA19 with involvement in *** A retrospective review of the medical records of 60 patients with stage IV CRC was conducted, assessing clinicopathological variables and progression-free survival(PFS). TCA19 gene expression was determined by quantitative polymerase chain reaction(qPCR) in matched normal and tumor tissues taken from the study cohort. Expression of potential immune-oncology regulatory proteins and targets was examined by immunohistochemistry(IHC), western blot, immunofluorescence staining in tissues from a validation cohort of 10 patients, and in CRC cell lines co-cultured with monocyte in *** In the patients with TCA19 score higher than the median, the PFS rates of eight patients who received the targeted regimens were significantly higher than the PFS rates of four patients who received 5-fluorouracil-based regimen(P = 0.041).In multivariate analysis, expression of signaling lymphocytic activation molecule family, member 7(SLAMF7) and triggering receptor expressed on myeloid cells 1(TREM1) was associated with PFS in the 60-patient cohort. After checking another 10 validate set, the expression of the IHC, the level of real-time qPCR,and the level of western blot were lower for SLAMF7 and higher for TREM7 in primary and metastatic tumors than in normal tissues. In CRC cells expressing SLAMF7 that were co-cultured with a monocytic cell line, levels of CD 68 and CD73 were significant

关键词： Colorectal cancer Prognosis Immunotherapy Signaling lymphocytic activation molecule family,member 7 Triggering receptor expressed on myeloid cells 1

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Effects of solid-state fermentation using R. oligosporus on the phytochemical composition of wild-simulated ginseng leaf and its biological properties

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Food Bioscience 2023年第2期52卷 532-540页

作者： Juho Lim Hayeong Kim Sung Bae Park Kunal Pal seung wook Kim Doman Kim Graduate School of International Agricultural Technology Seoul National UniversityGangwon-do25354Republic of Korea Institute of Food Industrialization Institutes of Green Bioscience and TechnologySeoul National UniversityGangwon-do25354Republic of Korea Biseondong Wild Ginseng Company Hongcheon-gunGangwon-do25162Republic of Korea Department of Biotechnology and Medical Engineering National Institute of TechnologyRourkela769008India Ottogi Corporation Anyang-siGyeonggi-do14060Republic of Korea Fervere Campus Corporation Gangwon-do25354Republic of Korea

Wild-simulated ginseng(WSG)grown in the mountains without specific facilities is widely used as functional foods in Asian countries due to its bioactive compounds,such as ***,long-time cultivation,lower survival rate,and small size of WSG limit its use in the food *** this study,WSG leaves,which can be harvested annually,were fermented using *** for 0-10 *** phytochemical compounds of fermented WSG leaves,including squalene,phenols,saponins,and most ginsenosides(except for Rb1),were increased.L-carnitine was newly synthesized in WSG leaves,with a content of 165.1μg/g measured after 10 days of ***,the minor ginsenosides Rg2,Rg3,Rh1,F1,F2,and CK increased by 1.32-,1.58-,1.42-,1.91-,1.49-,and 4.43-fold compared to non-fermented WSG *** highest antioxidant activities,as measured by FRAP and ORAC assays of WSG leaves,were obtained after 3 days of ***,the suppression of NO release in LPS-stimulated RAW264.7 cells and lipid accumulation in mouse 3T3-L1 cells of fermented WSG leaves was greater than in WSG *** these results,fermented WSG leaves have potential applications as functional materials in the food industry.

关键词： Wild-simulated ginseng leaf L-carnitine Solid-state fermentation R.oligosporus Ginsenosides Anti-inflammation Anti-lipid accumulation

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Intra-His bundle block in 2:1 atrioventricular block

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World Journal of Cardiology 2015年第10期7卷 700-702页

作者： seung Pyo hong Yon Woong Park Young Soo Lee Division of Cardiology Department of Internal Medicine Daegu Catholic University Medical Center

Intra-hisian atrioventricular(AV) block is not a common phenomenon, but it is important for the development of advanced or complete AV block. We observed a 77-yearold female patient with the 2:1 AV block due to an intrahisian block. In this case we tried to detect the block site, but an alternating pattern of the AH conduction was noted on the His-electrogram in the electrophysiological study(EPS). The cause of the confusing finding might have been the instability of the catheter to record a His potential. We could detect a splitting of the Hiselectrogram with an intra-hisian block after minimal manipulation of the catheter. The authors' observations suggest that catheter stability is important for a precise recording in the EPS and radiofrequency catheter ablation procedure.

关键词： Cardiac electrophysiologic techniques Bundle of Hi

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A Retroperitoneal Inflammatory Myofibroblastic Tumor Mimicking a Germ Cell Tumor of the Undescended Testis: A Case Report and Literature Review

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Advances in Computed Tomography 2016年第3期5卷 35-41页

作者： Seul-Bi Lee Jung-Hee Yoon seung-Ho Kim Yedaun Lee Jin-Soo Lee Jung-wook Seo Department of Radiology Inje University Haeundae Paik Hospital College of Medicine Busan South Korea Department of Radiology Inje University Ilsan Paik Hospital College of Medicine Goyang-si South Korea

We report here a case of an inflammatory myofibroblastic tumor in the retroperitoneum, which mimicked a germ cell tumor of the undescended testis. A 75-year-old healthy man presented with a palpable abdominal mass. On the computed tomography image, there was large, well-defined soft tissue mass in the left side of the retroperitoneum, and there was no visible left testis or seminal vesicle. After contrast enhancement, the mass appeared to be relatively homogeneous, considering its large size. With ultrasonography, it appeared as a well-defined, hypoechoic mass with intratumoral vascularity. This solid mass was surgically diagnosed as an inflammatory myofibroblastic tumor.

关键词： Inflammatory Myofibroblastic Tumor Computed Tomography Ultrasonography Retroperitoneal

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The current status of hormone treatment for prostate cancer patients in Korean real-world practice:a multi-institutional observational study

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Asian Journal of Andrology 2019年第2期21卷 115-120页

作者： Jung Kwon Kim Jung Jun Kim Taek Won Gang Tae Kyun Kwon hong Sup Kim seung Chui Park Jae-Shin Park Jong-Yeon Park Seok Joong Yoon Youn-Soo Jeon Jin Seon Cho Kwan Joong Joo Sung-Hoo hong Seok-Soo Byun the Korean Urological Oncology Society Department of Urology Seoul National University Bundang HospitalSeongnam 13620Korea Department of Urology Hallym University Sacred Heart HospitalAnyang 14068Korea Department of Urology Kangbuk Samsung HospitalSeoul 03181Korea Department of Urology Seoul St.Mary HospitalSeoul 06591Korea 不详 Department of Urology Chonnam National University HospitalGwangju 61469Korea School of Medicine Kyungpook National University Medical CenterDaegu 41404Korea Department of Urology Konkuk University Medical CenterSeoul 05080Korea Department of Urology Wonkwang University HospitalIksan 54538Korea Department of Urology Daegu Catholic University Medical CenterDaegu 41911Korea Department of Urology Ulsan UniversityGangneung Asan HospitalGangneung 25440Korea Department of Urology Chungbuk National University HospitalCheongju 28644Korea Department of Urology Soonchunhyang University HospitalCheonan 31151Korea

We aimed to evaluate the current nationwide trend,efficacy,safety,and quality of life(QoL)profiles of hormone treatment in real-world practice settings for prostate cancer(PCa)patients in Korea.A total of 292 men with any biopsy-proven PCa(TanyNanyMany)from 12 institutions in Korea were included in this multi-institutional,observational study of prospectively collected *** luteinizing hormone-releasing hormone(LHRH)agonists were allowed to be investigational *** was defined as(1)the rate of castration(serum testosterone≤50 ng dl^-1)at 4-week visit and(2)breakthrough(serum testosterone>50 ng dl^-1 after castration).Safety assessments included routine examinations for potential adverse events,laboratory tests,blood pressure,body weight,and bone min era I density(BMD,at baseli ne and at the last follow-up visit).QoL was assessed using the Expa nded Prostate Cancer Index Composite-26(EPIC-26).The most comm on initial therapeutic regimen was LHRH agonist with an ti-androge n(78.0%),and the most commonly used LHRH agonist for combination and mono therapy was leuprolide(64.0%for combi natio n and 58.0%for monotherapy).The castration and breakthrough rates were 78.4%and 6.6%,*** laboratory results related to dyslipidemia worsened after 4 weeks of hormone *** addition,the mean BMD T-score was significantly lower at the last follow-up(mean:-1.950)compared to baseline(mean:-0.195).The mean total EPIC-26 score decreased from 84.8(standard deviation[s.d.]:12.2)to 78.3(s.d.:8.1),with significant deterioration only in the urinary domain(mean:23.5 at baseli ne and 21.9 at the 4-week visit).These findi ngs dem on strate the n ationwide trend of current practice setti ngs in hormone treatment for PCa in Korea.

关键词： efficacy hormonal treatment Korean population prostate cancer safety trend

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