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Sensitivity and specificity of five malaria rapid diagnostic kits used in south-south region of Nigeria

Open Journal of Clinical Diagnostics 2012年第4期2卷 70-75页

作者： seto aladenika Mirabeau Tatfeng Gilbert Nwobu Eguagie Osakue Alfred Ehiaghe Joy Imuetinya Ehiaghe Maureen Uchechukwu Okwu Augustina Isioma Ikusemoro Igbinedion University Okada Okada Nigeria Igbinedion University Teaching Hospital Okada Okada Nigeria Laho Medical Center Benin City Nigeria Niger Delta University Wilberforce Island Nigeria

Sensitivity and specificity of five commonly used rapid malaria test kits in South-South geopolitical zone of Nigeria were analyzed, namely Plasmotest (Biotech International, Germany), Malariatest (Acon Diagnostic, USA), AcumPF (Acumen, China), PF test (Diaspot, Indonesia) and Malaria P.f. (Global, USA). A total of 200 positive malaria blood sample using microscopy as gold standard and 200 negative were obtained from adult and children. All the kits were tested against these gold standards as directed by the manufacturers. Of the 100 positive gold standard used from children sample, 96% sensitivity were recorded for Biotech, Acon and Global 97%, while Acumen and Diaspot had 99% and 98% respectively. The the highest Specificity was recorded with Biotech 99% both Acumen and Diaspot had 97% specificity. In adult blood sample, there was general reduction in the sensitivity of the test kits. We obtained Sensitivity of 30% with Biotech, 46% with Acon, Global 73%, Diaspot 75%, and Acumen 78%. Considering the increasing influx of MRDT’s into the country, and from the result obtained from this study, there is need for control agensis to ensure that diagnostic test kits be reevaluated before introduction into Nigeria market.

关键词： Malaria Rapid Malaria Kit Sensitivity Specificity

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Evaluation of Some Etiological Factors of Haemolytic Disease of the New Born in Ile-Ife

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Open Journal of Clinical Diagnostics 2014年第1期4卷 5-11页

作者： Johnson Kayode Fadairo seto Tunrayo aladenika Clement Osaiyuwu Mathew F. Olaniyan Kevin Aghatise Achievers University Owo Nigeria Igbinedion University Okada Nigeria

Heamolytic disease of the newborn is a neonatal disease associated with red cells heamolysis resulting from red cell antigens of the foetus and mother red cells antibodies. This study involved a cross sectional laboratory based analytical survey, eighty neonates with pyrexia of unknown origin and twenty neonates apparently healthy were selectected for this study. ABO and Rh blood group, Direct Coombs’ test, Glucose-6-phosphate dehydrogenase estimation and blood culture were performed on blood samples collected from each neonate. Blood group O had the highest number of neonates (60% control;61% test);80% and 81.3% of the control and test neonates are Rh positive respectively. None of the control subjects are positive to DCT while 5% of test subjects positive to DCT;4% and 8.7% of the control and test respectively are positive to G6-PD deficiency. The study recorded 0% growth in blood culture of control subjects while 2.5% of the neonates blood samples yielded when cultured. Though prevalence rate of HDN in our society is diminishing gradually based on this study, there is need to look inwardly to other aetiological factors that can cause HDN apart from blood group incompatibility, since sudden infant death is on increase now.

关键词： HDN G6-PD DCT Red Cell

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