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Potassium transporter OsHAK9 regulates seed germination under salt stress by preventing gibberellin degradation through mediating OsGA2ox7 in rice

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Journal of Integrative Plant Biology 2024年第4期66卷 731-748页

作者： Peng Zeng Ting Xie Jiaxin Shen Taokai Liang Lu Yin Kexin Liu Ying He Mingming Chen Haijuan Tang Sunlu Chen sergey shabala Hongsheng Zhang Jinping Cheng National Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization Jiangsu Collaborative Innovation Center for Modern Crop ProductionHainan Yazhou Bay Seed LabJiangsu Province Engineering Research Center of Seed Industry Science and TechnologyNanjing Agricultural UniversityNanjing 210095China International Research Center for Environmental Membrane Biology Foshan UniversityFoshan 528000China School of Biological Sciences University of Western AustraliaCrawley WA 6009Australia

Soil salinity has a major impact on rice seed germination,severely limiting rice production.Herein,a rice germination defective mutant under salt stress(gdss)was identified by using chemical mutagenesis.The GDSS gene was detected via MutMap and shown to encode potassium transporter OsHAK9.Phenotypic analysis of complementation and mutant lines demonstrated that OsHAK9 was an essential regulator responsible for seed germination under salt stress.OsHAK9 is highly expressed in germinating seed embryos.Ion contents and non-invasive micro-test technology results showed that OsHAK9 restricted K^(+)efflux in salt-exposed germinating seeds for the balance of K^(+)/Na^(+).Disruption of OsHAK9 significantly reduced gibberellin 4(GA4)levels,and the germination defective phenotype of oshak9a was partly rescued by exogenous GA_(3)treatment under salt stress.RNA sequencing(RNA-seq)and real-time quantitative polymerase chain reaction analysis demonstrated that the disruption of OsHAK9 improved the GA-deactivated gene OsGA2ox7 expression in germinating seeds under salt stress,and the expression of OsGA2ox7 was significantly inhibited by salt stress.Null mutants of OsGA2ox7 created using clustered,regularly interspaced,short palindromic repeat(CRISPR)/CRISPR-associated nuclease 9 approach displayed a dramatically increased seed germination ability under salt stress.Overall,our results highlight that OsHAK9 regulates seed germination performance under salt stress involving preventing GA degradation by mediating OsGA2ox7,which provides a novel clue about the relationship between GA and OsHAKs in rice.

关键词： GDSS gibberellins potassium transporter rice salt tolerance seed germination

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Rewilding staple crops for the lost halophytism:Toward sustainability and profitability of agricultural production systems

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Molecular Plant 2022年第1期15卷 45-64页

作者： Nishtha Rawat Silas Wungrampha Sneh L.Singla-Pareek Min Yu sergey shabala Ashwani Pareek Stress Physiology and Molecular Biology Lab School of Life SciencesJawaharlal Nehru UniversityNew Delhi 110067India Plant Stress Biology International Centre for Genetic Engineering and BiotechnologyNew Delhi 110067India International Research Centre for Environmental Membrane Biology Foshan UniversityFoshan 528000China Tasmanian Institute for Agriculture University of TasmaniaHobart Tas 7001Australia National Agri-Food Biotechnology institute Mohali 140306India

Abiotic stress tolerance has been weakened during the domestication of all major staple crops.Soil salinity is a major environmental constraint that impacts over half of the world population;however,given the increasing reliance on irrigation and the lack of available freshwater,agriculture in the 21st century will increasingly become saline.Therefore,global food security is critically dependent on the ability of plant breeders to create high-yielding staple crop varieties that will incorporate salinity tolerance traits and ac-count for future climate scenarios.Previously,we have argued that the current agricultural practices and reliance on crops that exclude salt from uptake is counterproductive and environmentally unsustainable,and thus called for a need for a major shift in a breeding paradigm to incorporate some halophytic traits that were present in wild relatives but were lost in modern crops during domestication.In this review,we provide a comprehensive physiological and molecular analysis of the key traits conferring crop halophy-tism,such as vacuolar Na+sequestration,ROS desensitization,succulence,metabolic photosynthetic switch,and salt deposition in trichomes,and discuss the strategies for incorporating them into elite germ-plasm,to address a pressing issue of boosting plant salinity tolerance.

关键词： halophyte salinity salt sequestration tissue tolerance trichomes water use efficiency

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Phosphoinositides:Emerging players in plant salinity stress tolerance

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Molecular Plant 2021年第12期14卷 1973-1975页

作者： sergey shabala Min Yu International Research Centre for Environmental Membrane Biology Foshan UniversityFoshan 528041China Tasmanian Institute of Agriculture University of TasmaniaHobartTas 7001Australia

Soil salinity reduces the biodiversity of natural ecosystems and severely limits sustainability and profitability of agricultural pro-duction systems.Given the increasing reliance of modern agricul-ture on irrigation(Liu et al.,2020a),the extent of the soil salinity is only going to increase under current climate trends scenarios.In this context,achieving global food security in the 21 st century will be not feasible without developing a salt-tolerant crop germplasm.However,the physiological and genetic complexity of this trait has significantly handicapped progress in the field.

关键词： soil salinity germplasm

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Transport Across Chloroplast Membranes： Optimizing Photosynthesis for Adverse Environmental Conditions

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Molecular Plant 2016年第3期9卷 356-370页

作者： Igor Pottosin sergey shabala Biomedicat Centre University of Colima Colima Colima 28045 Mexico School of Land and Food University of Tasmania Hobart TAS 7001 Australia

Chloroplasts are central to solar light harvesting and photosynthesis. Optimal chloroplast functioning is vitally dependent on a very intensive traffic of metabolites and ions between the cytosol and stroma, and should be attuned for adverse environmental conditions. This is achieved by an orchestrated regulation of a variety of transport systems located at chloroplast membranes such as porines, solute channels, ion-specific cation and anion channels, and various primary and secondary active transport systems. In this review we describe the molecular nature and functional properties of the inner and outer envelope and thylakoid membrane channels and transporters. We then discuss how their orchestrated regulation affects thylakoid structure, electron transport and excitation energy transfer, proton-motive force partition, ion homeostasis, stromal pH regulation, andvolume regulation. We link the activity of key cation and anion transport systems with stress-specific signaling processes in chloroplasts, and discuss how these signals interact with the signals generated in other organelles to optimize the cell performance, with a special emphasis on Ca^2＋ and reactive oxygen species signaling.

关键词： stroma thylakoid p.m.f. envelope reactive oxygen species programmed cell death

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K^+ retention in leaf mesophyll, an overlooked component of salinity tolerance mechanism:A case study for barley

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Journal of Integrative Plant Biology 2015年第2期57卷 171-185页

作者： Honghong Wu Min Zhu Lana shabala Meixue Zhou sergey shabala School of Land and Food University of Tasmania

Plant salinity tolerance is a physiologically complex trait, with numerous mechanisms contributing to it. In this work, we show that the ability of leaf mesophyll to retain K＋ represents an important and essentially overlooked component of a salinity tolerance mechanism. The strong positive correlation between mesophyll K＋ retention ability under saline conditions （quantified by the magnitude of NaCl-induced K＋ efflux from mesophyll） and the overall salinity tolerance （relative fresh weight and/or survival or damage under salinity stress） was found while screening 46 barley （Hordeum vulgare L.） genotypes contrasting in their salinity tolerance. Genotypes with intrinsically higher leaf K＋ content under control conditions were found to possess better K＋ retention ability under salinity and, hence, overall higher tolerance. Contrary to previous reports for barley roots, K＋ retention in mesophyll was not associated with an increased H＋-pumping in tolerant varieties but instead correlated negatively with this trait. These findings are explained by the fact that increased H＋ extrusion may be needed to charge balance the activity and provide the driving force for the high affinity HAK/KUP K＋ transporters required to restore cytosolic K＋ homeostasis in salt-sensitive genotypes.

关键词： Cytosolic K＋ homeostasis ion flux membrane potential membrane transport tissue tolerance

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Proto Kranz-like leaf traits and cellular ionic regulation are associated with salinity tolerance in a halophytic wild rice

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Stress Biology 2022年第1期2卷 587-605页

作者： Miing-Tiem Yong Celymar Angela Solis Samuel Amatoury Gothandapani Sellamuthu Raja Rajakani Michelle Mak Gayatri Venkataraman Lana shabala Meixue Zhou Oula Ghannoum Paul Holford Samsul Huda sergey shabala Zhong-Hua Chen School of Science Western Sydney UniversityPenrithNSW 2751Australia Tasmanian Institute of Agriculture College of Science and EngineeringUniversity of TasmaniaHobartTasmania 7001Australia Plant Molecular Biology Laboratory M.S.Swaminathan Research FoundationIII Cross StreetTaramani Institutional Area-600113ChennaiIndia Hawkesbury Institute for the Environment Western Sydney UniversityPenrithNSW 2751Australia International Research Centre for Environmental Membrane Biology Foshan UniversityFoshan 528000China

Species of wild rice(Oryza spp.)possess a wide range of stress tolerance traits that can be potentially utilized in breeding climate-resilient cultivated rice cultivars(Oryza sativa)thereby aiding global food security.In this study,we conducted a greenhouse trial to evaluate the salinity tolerance of six wild rice species,one cultivated rice cultivar(IR64)and one landrace(Pokkali)using a range of electrophysiological,imaging,and whole-plant physiological techniques.Three wild species(O.latifolia,O.officinalis and O.coarctata)were found to possess superior salinity stress tolerance.The underlying mechanisms,however,were strikingly different.Na+accumulation in leaves of O.latifolia,O.officinalis and O.coarctata were significantly higher than the tolerant landrace,Pokkali.Na+accumulation in mesophyll cells was only observed in O.coarctata,suggesting that O.officinalis and O.latifolia avoid Na+accumulation in mesophyll by allocating Na+to other parts of the leaf.The finding also suggests that O.coarctata might be able to employ Na+as osmolyte without affecting its growth.Further study of Na+allocation in leaves will be helpful to understand the mechanisms of Na+accumulation in these species.In addition,O.coarctata showed Proto Kranz-like leaf anatomy(enlarged bundle sheath cells and lower numbers of mesophyll cells),and higher expression of C4-related genes(e.g.,NADPME,PPDK)and was a clear outlier with respect to salinity tolerance among the studied wild and cultivated Oryza species.The unique phylogenetic relationship of O.coarctata with C4 grasses suggests the potential of this species for breeding rice with high photosynthetic rate under salinity stress in the future.

关键词： Gas exchange Gene expression Ion flux Na+imaging Oryza sativa Oryza coarctata

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Molecular mechanisms of salinity tolerance in rice

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The Crop Journal 2021年第3期9卷 506-520页

作者： Tianxiao Chen sergey shabala Yanan Niu Zhong-Hua Chen Lana shabala Holger Meinke Gayatri Venkataraman Ashwani Pareek Jianlong Xu Meixue Zhou Tasmanian Institute of Agriculture University of TasmaniaPrivate Bag 1375ProspectTAS 7250Australia Guangdong Laboratory of Lingnan Modern Agriculture Genome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120GuangdongChina Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural SciencesBeijing 100081China International Research Centre for Environmental Membrane Biology Foshan UniversityFoshan 528000GuangdongChina School of Science Hawkesbury Institute for the EnvironmentWestern Sydney UniversityPenrithNSW 2751Australia University of Tasmania Private Bag 51HobartTAS 7001Australia Plant Molecular Biology Laboratory M.S.Swaminathan Research FoundationⅢCross StreetTaramani Institutional AreaChennai 600113India School of Life Sciences Jawaharlal Nehru UniversityNew Delhi 110067India National Agri-Food Biotechnology Institute Mohali 140306India

Salinity is one of the major abiotic stresses which impose constraints to plant growth and production.Rice(Oryza sativa L.)is one of the most important staple food crops and a model monocot plant.Its production is expanding into regions that are affected by soil salinity,requiring cultivars more tolerant to saline conditions.Understanding the molecular mechanisms of such tolerance could lay a foundation for varietal improvement of salt tolerance in rice.In spite of extensive studies exploring the mechanism of salt tolerance,there has been limited progress in breeding for increased salinity tolerance.In this review,we summarize the information about the major molecular mechanisms underlying salinity tolerance in rice and further discuss the limitations in breeding for salinity tolerance.We show that numerous gene families and interaction networks are involved in the regulation of rice responses to salinity,prompting a need for a comprehensive functional analysis.We also show that most studies are based on whole-plant level analyses with only a few reports focused on tissue-and/or cell-specific gene expression.More details of salt-responsive channel and transporter activities at tissue-and cell-specific level still need to be documented before these traits can be incorporated into elite rice germplasm.Thus,future studies should focus on diversity of available genetic resources and,particular,wild rice relatives,to reincorporate salinity tolerance traits lost during domestication.

关键词： Oryza sativa L. Reactive oxygen species(ROS) Stomatal regulation Membrane transporters Osmotic adjustment Gene network Crosstalk

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Hypoxia-induced increase in GABA content is essential for restoration of membrane potential and preventing ROS-induced disturbance to ion homeostasis

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Plant Communications 2021年第3期2卷 105-116页

作者： Qi Wu Nana Su Xin Huang Jin Cui Lana shabala Meixue Zhou Min Yu sergey shabala International Research Centre for Environmental Membrane Biology Foshan UniversityFoshan 528000China Tasmanian Institute for Agriculture College of Science and EngineeringUniversity of TasmaniaHobartTAS 7001Australia Institute of Crop Germplasm and Biotechnology Provincial Key Laboratory of AgrobiologyJiangsu Academy of Agricultural SciencesNanjing 210014China College of Life Sciences Nanjing Agricultural UniversityNanjing 210095China

When plants are exposed to hypoxic conditions,the level of g-aminobutyric acid(GABA)in plant tissues increases by several orders of magnitude.The physiological rationale behind this elevation remains largely unanswered.By combining genetic and electrophysiological approach,in this work we show that hypoxia-induced increase in GABA content is essential for restoration of membrane potential and preventing ROS-induced disturbance to cytosolic K+homeostasis and Ca^(2+)signaling.We show that reduced O_(2) availability affects H+-ATPase pumping activity,leading to membrane depolarization and K+loss via outward-rectifying GORK channels.Hypoxia stress also results in H_(2)O_(2) accumulation in the cell that activates ROS-inducible Ca^(2+)uptake channels and triggers self-amplifying"ROS-Ca hub,"further exacerbating K^(+)loss via non-selective cation channels that results in the loss of the cell’s viability.Hypoxia-induced elevation in the GABA level may restore membrane potential by pH-dependent regulation of H^(+)-ATPase and/or by generating more energy through the activation of the GABA shunt pathway and TCA cycle.Elevated GABA can also provide better control of the ROS-Ca^(2+)hub by transcriptional control of RBOH genes thus preventing over-excessive H_(2)O_(2) accumulation.Finally,GABA can operate as a ligand directly controlling the open probability and conductance of K+efflux GORK channels,thus enabling plants adaptation to hypoxic conditions.

关键词： potassium homeostasis calcium signaling NADPH oxidase GORK H+-ATPase reactive oxygen species

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Mechanisms of Plant Responses and Adaptation to Soil Salinity

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The Innovation 2020年第1期1卷 69-109页

作者： Chunzhao Zhao Heng Zhang Chunpeng Song Jian-Kang Zhu sergey shabala Shanghai Center for Plant Stress Biology and CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of SciencesShanghai 200032China State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityKaifeng 475004China State Key Laboratory of Plant Molecular Genetics Shanghai Center for Plant Stress BiologyCenter of Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China Department of Horticulture and Landscape Architecture Purdue UniversityWest LafayetteIN 47907USA International Research Centre for Environmental Membrane Biology Foshan UniversityFoshan 528000China Tasmanian Institute of Agriculture University of TasmaniaHobartTAS 7001Australia

Soil salinity is a major environmental stress that restricts the growth and yield of crops.Understanding the physiological,metabolic,and biochemical responses of plants to salt stress and mining the salt tolerance-associated genetic resource in nature will be extremely important for us to cultivate salt-tolerant crops.In this review,we provide a comprehensive summary of the mechanisms of salt stress responses in plants,including salt stress-triggered physiological responses,oxidative stress,salt stress sensing and signaling pathways,organellar stress,ion homeostasis,hormonal and gene expression regulation,metabolic changes,as well as salt tolerance mechanisms in halophytes.Important questions regarding salt tolerance that need to be addressed in the future are discussed.

关键词： stress Plant salinity

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Barley responses to O2 deficiency during waterlogging:the importance of long-distance gas transport in membrane potential maintenance and K+ retention of root cells

Barley responses to O2 deficiency during waterlogging:the im...

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中国作物学会2013年学术年会

作者：曾凡荣张国平 sergey shabala School of Agricultural Science and Tasmanian Institute of Agriculture University of TasmaniaPrivate Bag 54HobartTAS 7001Australia Department of Agronomy College of Agriculture and BiotechnologyZhejiang University

O deficiency(hypoxia-anoxia)is one of the key determinants influencing root growth and functioning during waterlogging.Hypoxia-anoxia induced changes in net K flux and membrane potential across the plasma membrane of root cells were measured in elongation and mature zones of roots of two barley varieties contrasting in their waterlogging tolerance(tolerant variety CM72 and sensitive variety Naso Nijo)using the non-invasive

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