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Functional Interaction of the SNARE Protein NtSyp121 in Ca^2＋ Channel Gating, Ca^2＋ Transientsand ABA Signalling of Stomatal Guard Cells

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Molecular Plant 2008年第2期1卷 347-358页

作者： sergei Sokolovski Adrian Hills Robert A. Gay Michael R. Blatt Laboratory of Plant Physiology and Biophysics IBLS--Plant Sciences University of Glasgow Glasgow G12 8QQ UK Current address: Blackfriars 64 St Giles Oxford OX1 3LY UK

There is now growing evidence that membrane vesicle trafficking proteins, especially of the superfamily of SNAREs, are critical for cellular signalling in plants. Work from this laboratory first demonstrated that a soluble, inhibitory （dominant-negative） fragment of the SNARE NtSyp121 blocked K^＋ and CI^- channel responses to the stress-related hormone abscisic acid （ABA）, but left open a question about functional impacts on signal intermediates, especially on Ca^2＋-mediated signalling events. Here, we report one mode of action for the SNARE mediated directly through alterations in Ca^2＋ channel gating and its consequent effects on cytosolic-free [Ca^2＋] （[Ca^2＋]i） elevation. We find that expressing the same inhibitory fragment of NtSyp121 blocks ABA-evoked stomatal closure, but only partially suppresses stomatal closure in the presence of the NO donor, SNAP, which promotes [Ca^2＋]i elevation independently of the plasma membrane Ca^2＋ channels. Consistent with these observations, Ca^2＋ channel gating at the plasma membrane is altered by the SNARE fragment in a manner effective in reducing the potential for triggering a rise in [Ca^2＋]i, and we show directly that its expression in vivo leads to a pronounced suppression of evoked [Ca^2＋]i transients. These observations offer primary evidence for the functional coupling of the SNARE with Ca^2＋ channels at the plant cell plasma membrane and, because [Ca^2＋]i plays a key role in the control of K^＋ and CI^- channel currents in guard cells, they underscore an important mechanism for SNARE integration with ion channel regulation during stomatal closure.

关键词： Ca^2+ channel, hyperpolarization-activated abscisic acid membrane vesicle traffic cytosolic-free [Ca^2+]elevation Nicotiana plant pathogen defense.

The effect of self-focusing on laser space-debris cleaning

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Light(Science & Applications) 2014年第1期3卷 250-255页

作者： Alexander M Rubenchik Michail P Fedoruk sergei K Turitsyn Lawrence Livermore National Laboratory LivermoreCA 94550USA Institute of Computational Technologies Siberian Branch of the Russian Academy of SciencesNovosibirsk 630090Russia Novosibirsk State University Novosibirsk 630090Russia Aston Institute of Photonic Technologies School of Engineering and Applied ScienceAston UniversityBirmingham B47ETUK

A ground-based laser system for space-debris cleaning will use powerful laser pulses that can self-focus while propagating through the *** demonstrate that for the relevant laser parameters,this self-focusing can noticeably decrease the laser intensity on the *** show that the detrimental effect can be,to a great extent,compensated for by applying the optimal initial beam *** effect of laser elevation on the system performance is discussed.

关键词： laser self-focusing space debris

Impact of cavity symmetry on mode suppression and increase of free spectral range in solid-state dye microlaser

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Chinese Optics Letters 2007年第11期5卷 651-653页

作者： sergei Popov Sebastien Ricciardi Ari T.Friberg Sergey Sergeyev Royal Institute of Technology Stockholm 16440 Sweden Waterford Institute of Technology Waterford Ireland

We describe modeling the solid-state dye laser with the microcavity size comparable to light wavelength. Certain symmetry in the allocation of gain material leads to depletion of odd longitudinal modes that, in turn, ... 详细信息

关键词： mode Impact of cavity symmetry on mode suppression and increase of free spectral range in solid-state dye microlaser free

Pulsed corona discharge for improving treatability of coking wastewater

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Journal of Environmental Sciences 2018年第2期30卷 306-316页

作者： Ming Liu sergei Preis Iakov Kornev Yun Hu Chao-Hai Wei School of Environment and Energy South China University of Technology Guangzhou 510006 China Guangdong Provincial Engineering and Technology Research Centre for Environmental Risk Prevention and Emergency Disposal Guangzhou 510006 China Institute of High Technology Physics National Research Tomsk Polytechnic University 30 Lenin Ave. Tomsk 634050 Russian Federation

Coking wastewater（CW） contains toxic and macromolecular substances that inhibit biological treatment. The refractory compounds remaining in biologically treated coking wastewater（BTCW） provide chemical oxygen demand（COD） and color levels that make it unacceptable for reuse or disposal. Gas-phase pulsed corona discharge（PCD） utilizing mostly hydroxyl radicals and ozone as oxidants was applied to both raw coking wastewater（RCW） and BTCW wastewater as a supplemental treatment. The energy efficiency of COD,phenol, thiocyanate and cyanide degradation by PCD was the subject of the research. The cost-effective removal of intermediate oxidation products with addition of lime was also studied. The energy efficiency of oxidation was inversely proportional to the pulse repetition frequency： lower frequency allows more effective utilization of ozone at longer treatment times. Oxidative treatment of RCW showed the removal of phenol and thiocyanate at 800 pulses per second from 611 to 227 mg/L and from 348 to 86 mg/L, respectively, at 42 k Wh/m^3 delivered energy, with substantial improvement in the BOD5/COD ratio（from 0.14 to 0.43）.The COD and color of BTCW were removed by 30% and 93%, respectively, at 20 k Wh/m^3,showing energy efficiency for the PCD treatment exceeding that of conventional ozonation by a factor of 3–4. Application of lime appeared to be an effective supplement to the PCD treatment of RCW, degrading COD by about 28% at an energy input of 28 k Wh/m3 and the lime dose of 3.0 kg/m^3. The improvement of RCW treatability is attributed to the degradation of toxic substances and fragmentation of macromolecular compounds.

关键词： Coking wastewater Liming Low-temperature plasma Oxidation Ozone

Temperature regime of mountain permafrost in the Russian Altai Mountains

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Journal of Mountain Science 2022年第1期19卷 33-45页

作者： Robert SYSOLYATIN sergei SERIKOV Mikhail ZHELEZNYAK Mark SHATZ Yana TIKHONRAVOVA Melnikov Permafrost Institute Yakutsk 677010Russia

This study presents the long-term temperature monitoring in the Russian Altai Mountains. In contrast to the Mongolian and Chinese parts, the modern temperature regime of the Russian Altai remains unclear. The complexity of a comprehensive understanding of permafrost conditions in the Russian Altai is related to the high dissection of the terrain, the paucity of the latest observational data, and the sparse population of permafrost areas. The general objective of this study is to determine the temperature regime on the surface,in the active layer, and in the zero annual amplitude(ZAA) layer, based on the known patterns of permafrost distribution in the region. Using automatic measuring equipment(loggers), we obtained information on the temperature of frozen and thawed ground within the altitudes from 1484 to 2879 m a. s. l. during the period from 2014 to *** array of 15 loggers determined the temperature regime of bare and vegetated areas within watersheds,slopes, and valleys. N-factor parameters and surface temperature are similar to those in the Mongolian Altai, but the mean annual ground temperature at the depth of 1 m has a wide range of fluctuations(more than 32℃) based on research results, and we allocated it into three groups based on altitudinal zonality. Snow cover has a strong influence on the temperature regime, but the determination of the fine-scale variability requires additional *** temperature regime during the observation period remained stable, but continued monitoring allows a more detailed assessment of the response to climatic changes.

关键词： Ground temperature Mountain permafrost Temperature monitoring Altai Mountain Climate change

A new aberrant species of the genus Sphaerostoma (Trematoda: Opecoelidae) from the Amur River basin

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Zoological Systematics 2024年第4期49卷 352-360页

作者： Sergey G.Sokolov sergei V.Shchenkov Evgeniy V.Frolov sergei A.Vlasenkov A.N.Severtsov Institute of Ecology and Evolution RAS Moscow 119071Russia Department of Invertebrate Zoology St.Petersburg State UniversitySt.Petersburg 199034Russia Sakhalin Branch of Russian Federal Research Institute of Fisheries and Oceanography Yuzhno-Sakhalinsk 693023Russia

A new freshwater opecoelid species,Sphaerostoma tamarae ***.,is described from Rhynchocypris percnura mantschurica caught in the Syumnyur River(Amur River basin,Russia).This parasite species differs from all other congeners in the distribution of the vitelline follicles(restricted to the hindbody)and the morphology of the ventral sucker(strongly protuberant).Molecular phylogenetic analysis based on 28S rRNA gene sequences and the morphological analysis allow to tentatively assign the new species to the genus Sphaerostoma,without closing the question regarding the true genus affiliation of this *** addition,the authors provide an argument for renaming the Plagioporinae to the Sphaerostomatinae.

关键词： Rhynchocypris Plagioporinae Sphaerostomatinae minnow

Crystal-field levels of Nd3+ in a new langasite compound Nd3CrGe3Be2O14

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Journal of Rare Earths 2019年第11期37卷 1250-1254页

作者： sergei A.Klimin Boris V.Mill Marina N.Popova Institute of Spectroscopy Russian Academy of SciencesTroitskMoscow 108840Russia Faculty of Physics M.V.Lomonosov Moscow State UniversityMoscow 119991Russia

We report on the crystal-field(CF)levels of Nd^3+in Nd3CrGe3Be2O14,a representative of recently synthesized Be-containing *** are the first in the family to contain two magnetic subsystems,1 D chromium and Kagome-like rare-earth ***-resolution broad-band temperature-dependent spectra allow us to find all five CF Kramers doublets of the ground 4I9/2 CF multiplet and 38 levels of the excited 4I11/2,13/2,15/2,4F3/2,4F5/2+2H9/2,4F7/2+4S3/2 and 4F9/2 CF *** data form a basis for a future exploration of this interesting two-sublattice magnet with frustrated *** transitions between Kramers doublets of Nd^3+indicate magnetic activity of 1D-chromium chains at T<20 K.

关键词： Langasite Two magnetic subsystems High-resolution spectroscopy Crystal-field levels Magnetic correlations Rare earths

Gain through losses in nonlinear optics

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Light(Science & Applications) 2018年第1期7卷 641-651页

作者： Auro M.Perego sergei K.Turitsyn Kestutis Staliunas Aston Institute of Photonic Technologies Aston UniversityBirmingham B47ETUK Novosibirsk State University Novosibirsk 630090Russia InstitucióCatalana de Recerca i Estudis Avançats Pg.Lluis Companys 2308010 BarcelonaSpain Departament de Física i Enginyeria Nuclear Universitat Politècnica de CatalunyaRambla Sant Nebridi 2208222 TerrassaBarcelonaSpain

Instabilities of uniform states are ubiquitous processes occurring in a variety of spatially extended nonlinear *** instabilities are at the heart of symmetry breaking,condensate dynamics,self-organisation,pattern formation,and noise amplification across diverse disciplines,including physics,chemistry,engineering,and *** nonlinear optics,modulation instabilities are generally linked to the so-called parametric amplification process,which occurs when certain phase-matching or quasi-phase-matching conditions are *** the present review article,we summarise the principle results on modulation instabilities and parametric amplification in nonlinear optics,with special emphasis on optical *** then review state-of-the-art research about a peculiar class of modulation instabilities(MIs)and signal amplification processes induced by dissipation in nonlinear optical *** applied to certain parts of the spectrum counterintuitively lead to the exponential growth of the damped mode themselves,causing gain through *** discuss the concept of imaging of losses into gain,showing how to map a given spectral loss profile into a gain *** demonstrate with concrete examples that dissipation-induced MI,apart from being of fundamental theoretical interest,may pave the way towards the design of a new class of tuneable fibre-based optical amplifiers,optical parametric oscillators,frequency comb sources,and pulsed lasers.

关键词： nonlinear optics parametric

Second-line treatment of metastatic gastric cancer:Current options and future directions

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World Journal of Gastroenterology 2015年第41期21卷 11621-11635页

作者： Dheepak Kanagavel Mikhail Fedyanin Alexey Tryakin sergei Tjulandin Department of Clinical Pharmacology andChemotherapy N. N. Blokhin Russian Cancer Research Center

Gastric cancer remains one among the leading causes of cancer-related deaths, regardless of its decreasing incidence and newly available treatment options. Most patients present at an advanced stage and are treated with upfront systemic chemotherapy. Those patients receiving first-line therapy may initially respond to treatment, but many of them relapse over time. In such condition, second-line treatment for disease progression remains the only available option. Although there exists no standard approach in the second-line setting, several phase Ⅲ trials have shown modest survival benefit in patients receiving irinotecan, taxane and ramucirumab over the best supportive care or active agents. This review analyzes the currently available treatment regimens and future directions of research in the second-line setting for metastatic gastric cancer with the best available evidence. Additionally, the prognostic factors that influence patient survival in those receiving second-line therapy are discussed.

关键词： Metastatic gastric cancer Second-line chemotherapy