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Function of hepatocyte spheroids in bioactive microcapsules is enhanced by endogenous and exogenous hepatocyte growth factor

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Bioactive Materials 2023年第10期28卷 183-195页

作者： Kihak Gwon Daheui Choi Jose Mde Hoyos-Vega Harihara Baskaran Alan M.Gonzalez-Suarez seonhwa lee Hye Jin Hong Kianna M.Nguyen Ether Dharmesh Go Sugahara Yuji Ishida Takeshi Saito Gulnaz Stybayeva Alexander Revzin Department of Physiology and Biomedical Engineering Mayo ClinicRochesterMNUSA Department of Chemical and Biomolecular Engineering Case Western Reserve UniversityClevelandOHUSA Biomedical Engineering Saint Louis UniversitySt.LouisMOUSA University of Southern Californi1 Keck School of MedicineDepartment of MedicineDivision of Gastrointestinal and Liver DiseasesLos AngelesCAUSA Research and Development Department PhoenixBioCo.LtdKagamiyam1Higashi-Hiroshim1Hiroshim1Japan USC Research Center for Liver Diseases Los AngelesCAUSA

The ability to maintain functional hepatocytes has important implications for bioartificial liver development,cell-based therapies,drug screening,and tissue engineering.Several approaches can be used to restore hepatocyte function in vitro,including coating a culture substrate with extracellular matrix(ECM),encapsulating cells within biomimetic gels(Collagen-or Matrigel-based),or co-cultivation with other cells.This paper describes the use of bioactive heparin-based core-shell microcapsules to form and cultivate hepatocyte spheroids.These microcapsules are comprised of an aqueous core that facilitates hepatocyte aggregation into spheroids and a heparin hydrogel shell that binds and releases growth factors.We demonstrate that bioactive microcapsules retain and release endogenous signals thus enhancing the function of encapsulated hepatocytes.We also demonstrate that hepatic function may be further enhanced by loading exogenous hepatocyte growth factor(HGF)into microcapsules and inhibiting transforming growth factor(TGF)-β1 signaling.Overall,bioactive microcapsules described here represent a promising new strategy for the encapsulation and maintenance of primary hepatocytes and will be beneficial for liver tissue engineering,regenerative medicine,and drug testing applications.

关键词： Droplet microfluidics Bioactive microcapsules Hepatocyte spheroid Heparin Hepatocyte growth factor

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Hemodynamic instability following intravenous dexmedetomidine infusion for sedation under brachial plexus block: Two case reports

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World Journal of Clinical Cases 2023年第30期11卷 7469-7474页

作者： Ye Sull Kim Chanhong lee Jeongmin Oh seonhwa Nam A Ram Doo Department of Anesthesiology and Pain Medicine Jeonbuk National University HospitalJeonju 54907South Korea Department of Anesthesiology and Pain Medicine Jeonbuk National University Medical School and HospitalJeonju 54907South Korea Department of Anesthesiology and Pain Medicine Research Institute of Clinical Medicine-Biomedical Research Institute of Jeonbuk National University HospitalJeonju 54907South Korea

BACKGROUND Dexmedetomidine(DMED)is frequently used as a sedative in several medical fields.The benefits of DMED include enhanced quality of regional anesthesia,prolonged analgesia,and postoperative opioid-sparing when administered intravenously or perineurally in combination with regional anesthesia.Severe hemodynamic complications,such as profound bradycardia and hypotension,can occur after DMED administration in critically ill patients or overdosage;however,there are few reports of complications with DMED administration following brachial plexus block(BPB).CASE SUMMARY We present two cases of hemodynamic instability that occurred following the initial loading of DMED under supraclavicular BPB.A healthy 29-year-old man without any medical history showed profound bradycardia after receiving a loading dose of DMED 0.9μg/kg for 9 min.DMED administration was promptly stopped,and after receiving a second dose of atropine,the heart rate recovered.A 62-year-old woman with a history of cardiomyopathy became hypotensive abruptly,requiring the administration of inotrope and vasopressors after receiving a reduced loading dose of 0.5μg/kg for 10 min.Half of the recommended loading dose of DMED was administered due to the underlying heart dysfunction.Decrea-sed blood pressure was maintained despite the intravenous administration of ephedrine.With continuous infusion of dopamine and norepinephrine,the vital signs were maintained within normal ranges.Inotropic and vasopressor support was required for over 6 h after the initial loading dose of DMED.CONCLUSION DMED administration following BPB could trigger hemodynamic instability in patients with decreased cardiac function as well as in healthy individuals.

关键词： Dexmedetomidine Brachial plexus block Profound bradycardia Complication Hypotension Instability Case report

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Evaluation of an erosion-sediment transport model for a hillslope using laboratory flume data

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Journal of Arid Land 2014年第6期6卷 647-655页

作者： Anya Catherine CARGUELLES MinJae JUNG Kristine Joy BMALLARI GiJung PAK Hafzullah AKSOY Levent M KAVVAS Ebru ERIS JaeYoung YOON YoungJoon lee seonhwa HONG Department of Environmental Engineering Korea University Sejong 339-700Korea Program in Environmental Technology and Policy Korea University Sejong 339-700Korea Department of Civil Engineering Istanbul Technical University Maslak 34469IstanbulTurkey Department of Civil and Environmental Engineering University of California DavisCalifornia 95616USA Department of Civil Engineering Ege University Bornova 35100IzmirTurkey Geum-River Environment Research Center National Institute of Environmental Research OkcheonChungbuk 373-804Korea

Climate change can escalate rainfall intensity and cause further increase in sediment transport in arid lands which in turn can adversely affect water quality. Hence, there is a strong need to predict the fate of sediments in order to provide measures for sound erosion control and water quality management. The presence of micro- topography on hillslopes influences processes of runoff generation and erosion, which should be taken into account to achieve more accurate modelling results. This study presents a physically based mathematical model for erosion and sediment transport coupled to one-dimensional overland flow equations that simulate rainfall-runoff generation on the rill and interrill areas of a bare hillslope. Modelling effort at such a fine resolution considering the flow con- nection between Jnterrill areas and rills is rarely verified. The developed model was applied on a set of data gath- ered from an experimental setup where a 650 cm×136 cm erosion flume was pre-formed with a longitudinal rill and interrJll having a plane geometry and was equipped with a rainfall simulator that reproduces natural rainfall characteristics. The flume can be given both longitudinal and lateral slope directions. For calibration and validation, the model was applied on the experimental results obtained from the setup of the flume having 5% lateral and 10% longitudinal slope directions under rainfall intensities of 105 and 45 mm/h, respectively. Calibration showed that the model was able to produce good results based on the R2 （0.84） and NSE （0.80） values. The model performance was further tested through validation which also produced good statistics （R2=0.83, NSE=0.72）. Results in terms of the sedigraphs, cumulative mass curves and performance statistics suggest that the model can be a useful and an important step towards verifying and improving mathematical models of erosion and sediment transport.

关键词： climate change erosion rill and interrill physically based model sediment transport

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