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Expression and Role of Voltage-Gated Sodium Channels in Human Dorsal Root Ganglion Neurons with Special Focus on Nav1.7,Species Differences, and Regulation by Paclitaxel

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Neuroscience Bulletin 2018年第1期34卷 4-12页

作者： Wonseok Chang Temugin Berta yong Ho Kim Sanghoon lee seok-yong lee Ru-Rong Ji Department of Anesthesiology Duke University Medical Center Durham NC 27710 USA Department of Physiology and Biophysics College of Medicine Eulji University Daejeon Korea Pain Research Center Department of Anesthesiology University of Cincinnati Medical Center Cincinnati OH 45267 USA D epartment of Physiology College of Medicine Gachon University Incheon Korea Department of Biochemistry Duke University Medical Center Durham NC 27710 USA Department of Neurobiology Duke University Medical Center Durham NC 27710 USA

Voltage-gated sodium channels （Navs） play an important role in human pain sensation. However, the expression and role of Nav subtypes in native human sensory neurons are unclear. To address this issue, we obtained human dorsal root ganglion （hDRG） tissues from healthy donors. PCR analysis of seven DRG-expressed Nav subtypes revealed that the hDRG has higher expression of Navl.7 （,-~ 50% of total Nav expression） and lower expres- sion of Navl.8 （~ 12%）, whereas the mouse DRG has higher expression of Nav 1.8 （- 45%） and lower expression of Navl.7 （- 18%）. To mimic Nav regulation in chronic pain, we treated hDRG neurons in primary cultures with paclitaxel （0.1-1 μmol/L） for 24 h. Paclitaxel increased the Navl.7 but not Navl.8 expression and also increased the transient Na＋ currents and action potential firing frequency in small-diameter （〈50 ~tm） hDRG neurons. Thus, the hDRG provides a translational model in which to study ＂human pain in a dish＂ and test new pain therapeutics.

关键词： Dorsal root ganglion Neuropathic pain Paclitaxel Voltage-gated sodium channels

Evaluating the Discriminatory Power of DNA Barcodes in Panicoideae, Poaceae

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Journal of Agricultural Science and Technology(B) 2014年第7期4卷 533-544页

作者： Jeongran lee Chang-seok Kim In-yong lee Department of Crop Life Safety National Academy of Agricultural Science Rural Development Administration Suwon 441- 701Republic of Korea

DNA barcoding is a powerful technique for species identification with little morphological knowledge, by using short sections of DNA from a specific region of the genome. Two core barcode markers, rbcL and matK, and a supplementary nuclear ribosomal internal transcribed spacer region were used to examine the effectiveness of the markers for Poaceae barcoding using 133 individuals of 36 taxa across 23 genera of Korean Panicoideae. We also aimed to establish a DNA barcode database for the major weeds of Korean Panicoideae. All three markers revealed a good level of amplification and sequencing success. As a single DNA marker, the ITS region achieved the highest species resolution, followed by matK. Resolving power was increased when nrlTS was incorporated into the core barcode markers. The best resolving power was obtained with a combination of matK ＋ ITS with 89.7%, followed by rbcL ＋ matK ＋ ITS with 89.3%. Thus, rbcL may be not necessary as a DNA barcode for Panicoideae species identification, when considering cost and effectiveness. Instead, a combination of matK ＋ ITS is proposed as the most suitable DNA barcode for the species identification of Panicoideae, Poaceae. We conclude that DNA barcoding using a combination of matK ＋ ITS could be one of powerful techniques for the identification of Poaceae species, The barcode sequences were deposited to the National Center for Biotechnology Information （NCBI） database for public use.

关键词： Plant barcode Poaceae core barcode markers nrITS resolving power.

Differential Inhibition of Nav1.7 and Neuropathic Pain by Hybridoma-Produced and Recombinant Monoclonal Antibodies that Target Nav1.7

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Neuroscience Bulletin 2018年第1期34卷 22-41页

作者： Sangsu Bang Jiho Yoo Xingrui Gong Di Liu Qingjian Han Xin Luo Wonseok Chang Gang Chen Sang-Taek Im yong Ho Kim Judith A.Strong Ma-Zhong Zhang Jun-Ming Zhang seok-yong lee Ru-Rong Ji Department of Anesthesiology Duke University Medical Center Department of Biochemistry Duke University Medical Center Pain Research Center Department of AnesthesiologyUniversity of Cincinnati Department of Anesthesiology Shanghai Children's Medical Center Department of Physiology and Biophysics College of Medicine Eulji University Department of Physiology College of Medicine Gachon University

Abstract The voltage-gated Na＋ channel subtype Nav1.7 is important for pain and itch in rodents and humans. We previously showed that a Nav1.7-targeting monoclonal antibody （SVmab） reduces Na＋ currents and pain and itch responses in mice. Here, we investigated whether recom- binant SVmab （rSVmab） binds to and blocks Nav1.7 similar to SVmab. ELISA tests revealed that SVmab was capable of binding to Nav1.7-expressing HEK293 cells, mouse DRG neurons, human nerve tissue, and the voltagesensor domain II of Nav1.7. In contrast, rSVmab showed no or weak binding to Nav1.7 in these tests. Patch-clamp recordings showed that SVmab, but not rSVmab, markedly inhibited Na＋ currents in Nav1.7-expressing HEK293 cells. Notably, electrical field stimulation increased the blocking activity of SVmab and rSVmab in Nav1.7- expressing HEK293 cells. SVmab was more effective than rSVmab in inhibiting paclitaxel-induced mechanical allodynia. SVmab also bound to human DRG neurons and inhibited their Na＋ currents. Finally, potential reasons for the differential efficacy of SVmab and rSVmab and future directions are discussed.

关键词： Primary afferent neuron Patch clamping (electrophysiology) Sodium channels Animal models

CONSTRUCTION OF BIOINFORMATICS SYSTEM

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CONSTRUCTION OF BIOINFORMATICS SYSTEM

Prominent cycling reversibility and kinetics enabled by CaTiO3 protective layer on Zn metal for aqueous Zn-ion batteries

Ⅸ International Congress on Medical and Applied Malacology

作者： Se Won Kang Yeon Soo Han Weon-Gyu Kho yong seok lee Department of Biology College of Natural Sciences Soonchunhyang UniversityAsan Chungnam336-745 Korea College of Agriculture and Life Science Chonnam National University 300 Yongbong-DongPuk-GuGwangju 500-757Korea Dept.of Parasitology Inje University College of Medicine and Dept.of Malariology PICRGaegum-dongBusanjin-gu Busan 614-735Korea Dept.of Parasitology Inje University College of Medicine and Dept.of Malariology PICRGaegum-dongBusanjin-gu Busan 614-735Korea

＜正＞To efficiently handle the massive sequences, a bioinformatic system was established. The constructed systems consisted of main server, assistance server, terminal server for primer working and gap closing, etc), and web server for database and BLAST server. Main and assistance servers were build up using SAMBA protocol for

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Journal of Energy Chemistry 2025年第1期100卷 245-258页

作者： Gaeun lee yong Nam Ahn Seongjoon So Chanwoo Park Jong-Seong Bae Taehyun Park Il Tae Kim Jaehyun Hur School of Chemical Biological and Battery Engineering Gachon University Seongnam Busan Center Korea Basic Science Institute

Aqueous Zn-ion batteries （AZIBs） have received considerable attention owing to their various advantages such as safety,low cost,simple battery assembly conditions,and high ionic ***,they still suffer from serious problems,including uncontrollable dendrite growth,corrosion,hydrogen evolution reaction （HER） from water decomposition,electrode passivation,and unexpected *** creation of a uniform artificial nanocrystal layer on the Zn anode surface is a promising strategy for resolving these ***,we propose the use of a perovskite CaTiO3（CTO） protective layer on Zn（CTO@Zn） as a promising approach for improving the performance of *** CTO artificial layer provides an efficient pathway for Zn ion diffusion towards the Zn metal because of the high dielectric constant （εr=180） and ferroelectric characteristics that enable the alignment of dipole moments and redistribute the Zn2+ions in the CTO *** avoiding the direct contact of the Zn anode with the electrolyte solution,the uneven dendrite growth,corrosion,parasitic side reactions,and HER are mitigated,while CTO retains its mechanical and chemical robustness during ***,CTO@Zn demonstrates an improved lifespan in a symmetric cell configuration compared with bare ***@Zn shows steady overpotential （～68 m V） for 1500 h at 1 mA cm-2/0.5 mA h cm-2,excelling bare ***,when paired with the V2O5-C cathode,the CTO@Zn//V2O5-C full battery delivers 148.4 mA h g-1（based on the mass of the cathode） after 300 *** study provides new insights into Zn metal anodes and the development of high-performance AZIBs.

关键词： Artificial layer Dielectric constant Zn metal anode Zinc aqueous battery

Novel acoustic features for speech emotion recognition

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Science China(Technological Sciences) 2009年第7期52卷 1838-1848页

作者： ROH yong-Wan KIM Dong-Ju lee Woo-seok HONG Kwang-seok School of Information and Communication Engineering Sungkyunkwan University300Chunchun-dongJangan-guSuwonKyungki-do440-746Korea Development Division / DSP Development Team Tamul Multimedia10th Fl.Anyang Trade Center1107Bisan-DongDongAn-GuAnyang-City431-050Korea

This paper focuses on acoustic features that effectively improve the recognition of emotion in human *** novel features in this paper are based on spectral-based entropy parameters such as fast Fourier transform(FFT) spectral entropy,delta FFT spectral entropy,Mel-frequency filter bank(MFB) spectral entropy,and Delta MFB spectral ***-based entropy features are *** reflect frequency characteristic and changing characteristic in frequency of *** implement an emotion rejection module using the probability distribution of recognized-scores and *** reduces the false recognition rate to improve overall ***-scores and rejected-scores refer to probabilities of recognized and rejected emotion recognition results,*** scores are first obtained from a pattern recognition *** pattern recognition phase uses the Gaussian mixture model(GMM).We classify the four emotional states as anger,sadness,happiness and *** proposed method is evaluated using 45 sentences in each emotion for 30 subjects,15 males and 15 *** results show that the proposed method is superior to the existing emotion recognition methods based on GMM using energy,Zero Crossing Rate(ZCR),linear prediction coefficient(LPC),and pitch *** demonstrate the effectiveness of the proposed *** of the proposed features,combined MFB and delta MFB spectral entropy improves performance approximately 10% compared to the existing feature parameters for speech emotion recognition *** demonstrate a 4% performance improvement in the applied emotion rejection with low confidence score.

关键词： speech emotion recognition MFB spectral entropy entropy emotion recognition rejection

Efficient thermal management and all-season energy harvesting using adaptive radiative cooling and a thermoelectric power generator

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Journal of Energy Chemistry 2023年第9期84卷 496-501页

作者： Chanil Park Woohwa lee Choyeon Park Sungmin Park Jaeho lee yong seok Kim Youngjae Yoo Advanced Materials Division Korea Research Institute of Chemical Technology(KRICT)Daejeon 34114Republic of Korea Department of Mechanical and Aerospace Engineering University of CaliforniaIrvineCA 92617United States Department of Advanced Materials Engineering Chung-Ang UniversityAnseong 17546Republic of Korea

Passive daytime radiative cooling(PDRC) is useful for thermal management because it allows an object to emit terrestrial heat into space without the use of additional *** produce sub-ambient temperatures under direct sunlight,PDRC materials are designed to reduce their absorption of solar energy and to enhance their long-wavelength infrared(LWIR) *** recent years,many photonic structures and polymer composites have been studied to improve the cooling system of ***,in cold weather(i.e. during winter in cold climates),buildings need to be kept warm rather than cooled due to heat *** overcome this limitation,temperature-responsive radiative cooling is a promising *** the present study,adaptive radiative cooling(ARC) film fabricated from a polydimethylsiloxane/hollow SiO_(2) microsphere/thermochromic pigment composite was *** found that the ARC film absorbed solar radiation under cold conditions while exhibiting radiative cooling at ambient temperatures above 40℃.Thus,in outdoor experiments,the ARC film achieved sub-ambient temperatures and had a theoretical cooling power of 63.2 W/m~2 in hot *** also demonstrated that radiative cooling with an energy harvesting system could be used to improve the energy management of buildings,with the thermoelectric module continuously generating output power using the ARC ***,we believe that our proposed ARC film can be employed for efficient thermal management of buildings and all-season energy harvesting in the near future.

关键词： Thermal management Daytime radiative cooling Temperature-adaptive film Thermoelectric device Energy harvesting

Spectrum of final pathological diagnosis of gastric adenoma after endoscopic resection

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World Journal of Gastroenterology 2011年第47期17卷 5177-5183页

作者： Kwan Woo Nam Kyu Sang Song Heon Young lee Byung seok lee Jae Kyu Seong seok Hyun Kim Hee seok Moon Eaum seok lee Hyun yong Jeong Gastroenterology Center Sun Hospital Pathologic Department Chung-Nam National University Hospital Gastroenterology Unit of Internal Medicine Department Chung-Nam National University Hospital

AIM: To investigate how many discrepancies occur in patients before and after endoscopic treatment of referred adenoma and the reason for these results. METHODS: We retrospectively reviewed data from 554 cases of 534 patients who were referred from primary care centres for adenoma treatment and treated for endoscopic mucosal resection (EMR) or endoscopic submucosal dissection (ESD) at Chungnam National University Hospital, from July 2006 to June 2009. Reendoscopy was examined in 142 cases and biopsywas performed in 108 cases prior to treatment. Three endoscopists (1, 2 and 3) performed all EMRs or ESDs and three pathologists (1, 2 and 3) diagnosed most of the cases. Transfer notes, medical records and endoscopic pictures of these cases were retrospectively reviewed and analyzed. RESULTS: Adenocarcinoma was 72 (13.0%) cases in total 554 cases after endoscopic treatment of referred adenoma. When the grade of dysplasia was high (55.0%), biopsy number was more than three (22.7%), size was no smaller than 2.0 cm (23.2%), morphologic type was depressed (35.8%) or yamada type Ⅳ (100%), and color was red (30.9%) or mixed-or-undetermined (25.0%), it had much more malignancy rate than the others (P < 0.05). All 18 cases diagnosed as adenocarcinoma in the re-endoscopic forceps biopsy were performed by endoscopist 1. There were different malignancy rates according to the pathologist (P = 0.027). CONCLUSION: High grade dysplasia is the most important factor for predicting malignancy as a final pathologic diagnosis before treating the referred gastric adenoma. This discrepancy can occur mainly through inappropriately selecting a biopsy site where cancer cells do not exist, but it also depends on the pathologist to some extent.

关键词： Discrepancy Adenoma High grade dyspla-sia Endoscopic mucosal resection Endoscopic submu-cosal dissection

Early recovery process and restoration planning of burned pine forests in central eastern Korea

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Journal of Forestry Research 2019年第1期30卷 243-255页

作者： Ji Hong An Chi Hong Lim yong Chan Cho Chang seok lee Department of Bio and Environmental Technology Seoul Women's University Division of Plant Conservation Korea National Arboretum

In central eastern Korea, there has been a continued effort to sustain pine forests because of their value for wood and mushroom production, as well as for other resources. Through the analysis of previously burned areas,we clarified the effects of fire on vegetation dynamics of pine forests by measuring changes in species composition,population structure, and the growth rates of major tree species. Vegetation analysis was conducted on a series of 100 m^2 plots established in Korean red pine(Pinus densiflora Siebold. & Zucc.) and Mongolian oak(Quercus mongolica Fisch. Ex Ledeb.) forests situated in three different topographic positions on slopes where fire severity varied. We confirmed that most pine trees will succumb to even a surface fire, whereas oak may be killed following high intensity or infrequent fires but will sprout and become dominant. Complete protection from fire favors other, more shade-tolerant deciduous hardwoods. Different restoration plans apply, based on observed vegetation responses for each topographic zone. Near the ridge top,where pine seed sources are most limited, it may be necessary to restore pine forests by planting and to control competing shrubs and herbs. At the mountain foot, where exotic species and species modified by hybridization have invaded aggressively, restoration that involves the introduction of natural vegetation is required. Mid-slope sites,where pine has been replaced by natural vegetation dominated by Mongolian oak, do not require any form of restorative treatment.

关键词： Vegetation succession Vegetation type Fire severity Fire disturbance Landscape structure