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ROLE OF FUNGAL LACCASE AND LOW MOLECULAR MEDIATORS ON DECOLOURIZATION OF AROMATIC DYES IN PAPER MILL EFFLUENTS

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天津科技大学学报 2004年第A2期19卷 148-155页

作者： Nam-seok cho Tae-Ho choi Woonsup Shin A. Leonowicz Professor School of Forest Resources Chungbuk National University Cheongju 361-763 KoreaProfessor School of Forest Resources Chungbuk National University Cheongju 361-763 KoreaProfessor Department of Chemistry Sogang University Seoul 121-742 KoreaProfessor Department of Biochemistry Marie Curie-Sklodowska University Lublin Poland

Several wood rotting fungi decolourized Remazol brilliant blue R (RBBR) and carminic acid (CA). Parallel activity of laccase in these fungi was studied. The addition of acetovanillone (AV) or acetosyringone (AS) intensified these processes: decolourization was more extensive than in the experiment omitting these compounds. At the presence of AS the decourization was more extensive than AV. However the level of decolorizing was relatively low in comparison to laccase activity on syringaldazine. The highly purified constitutive form of Cerrena unicolor and inducible form of Trametes versicolor laccases also destained both dyes. Anyway the addition of AV and AS improved the efficiency of dyes decolourization by wood rotting fungi and fungal laccase. Nitrogen starvation induced the laccase and decoloration activity in both organisms, irrespective of nitrogen availability. This fact indicates laccase not solely responsible for discoloration, and probably discoloration of dyes involves more than one mechanism.

关键词：真菌虫漆酶脱色芳香染料造纸厂废水处理木蚀真菌低分子真菌

Efficacy and safety of 0.4 percent sodium hyaluronate for endoscopic submucosal dissection of gastric neoplasms

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World Journal of Gastroenterology 2013年第20期19卷 3069-3076页

作者： Young Dae Kim Jun Lee Ju Yeon cho seok Won Kim Seong Hwan Kim Young Kwan cho Jin seok Jang Ji Sun Han Joo Young cho Division of Gastroenterology and Hepatology Department of Internal MedicineMedical SchoolChosun UniversityGwangju 501-717South Korea Department of Internal Medicine Eulji HospitalEulji UniversitySeoul 139-872South Korea Department of Internal Medicine Dong A University College of MedicineBusan 602-715South Korea Department of Internal Medicine Institute for Digestive ResearchDigestive Disease CenterSoonchunhyang University College of MedicineSeoul 140-743South Korea

AIM:To evaluate the efficacy and safety of sodium hyaluronate solution(SH) in endoscopic submucosal dissection(ESD) of gastric ***:A prospective multicenter randomized,double blind,controlled trial was designed and utilized in this study.A total of 76 patients with 5-20 mm sized gastric neoplasms were enrolled at three academic hospitals in South Korea from June 2011 to October *** were randomly assigned to the 0.4% sodium hyaluronate or control *** lesions underwent endoscopic *** was performed with 0.4%SH and normal saline(NS) solution for submucosal *** was assessed using en bloc resection and the number of additional *** evaluation variables were the volume of injection material,steepness of mucosal elevation,bleeding rate,procedural time and operator ***,the safety was assessed by analyzing adverse events during the ***:The usefulness rate in the 0.4%SH group and the controlled group had statistically significant difference under intention to treat(ITT) analysis(90.91% vs 61.11% P = 0.0041).Under per protocol(PP),the usefulness rate is statistically significant different(93.10% vs 61.76%,P = 0.0036).The difference in volume of the solution injected between 0.4%SH group and the controlled group and NS group was also statistically significant under intention to treat and per protocol analysis(ITT:0.03 ± 0.02 mL vs 0.06 ± 0.03 mL,P = 0.0003,PP:0.03 ± 0.02 mL vs 0.06 ± 0.03 mL,P = 0.0004).Satisfaction above the grade good was significantly higher in the SH group under intention to treat and per protocol analysis(ITT:90.91% vs 61.11%,P = 0.0041,PP = 93.11% vs 61.77%,P = 0.0022).Adverse events above grade 3 were not noticed in either *** adverse events were treated and were judged as not associated with the submucosal injection ***:0.4%SH solution is a safe and effective agent that doesn't cause any significant adverse events and is useful for submucos

关键词： Sodium hyaluronate Endoscopic submucosal dissection Gastric neoplasm Endoscopic mucosal resection

Protein profiling of Modulators regulated by E7 oncogene in HaCaT Keratinocytes by Approach to Omics:The effect of E7 on oxidative stress

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Protein profiling of Modulators regulated by E7 oncogene in ...

Current status and challenges in sentinel node navigation surgery for early gastric cancer

24th International Papillomavirus Conference and Clinical Workshop

作者： Sue-Nie Park Jin-seok Kang Soo-Kyung Suh Eun-Jin Kim Nin-Chul cho Jung-Hyun Shim Seong-goo Park Do-Young Yoon National Institute of Toxicological Research Korea Food and Drug Administration National Institute of Toxicological Research Korea Food and Drug Administration National Institute of Toxicological Research Korea Food and Drug Division of Bioscience and Biotechnology Konkuk University Division of Bioscience and Biotechnology Konkuk University Division of Bioscience and Biotechnology Konkuk University

＜正＞Cervical carcinoma is predominant cancer among malignancies in women throughout the world and human papillomavirus （HPV）16 is the most common agent linked to humman cervical *** HPV E7 oncoprotein can form a specific complex with a retinoblastoma tumor supressor gene product（p105-Rb）that results in the release of the E2F transcription factor,which

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Chinese Journal of Cancer Research 2017年第2期29卷 93-99页

作者： Bang Wool Eom Young-II Kim Hong Man Yoon Soo-Jeong cho Jong Yeul Lee Chan GyooKim Soo Jin Kim Ji Yoon Rho seok Ki Kim Myeong-Cherl Kook Young-Woo Kim Keun Won center for gastric cancer national cancer centerrepublic of korea center for diagnostic oncology national cancer centerrepublic of korea

Although a number of feasibility studies for sentinel node （SN） concepts in gastric cancer have been conducted since 2000, there remains a debate regarding detailed detection techniques and oncological safety. Two important multicenter phase II clinical trials were performed in Japan that used different methods and reached different conclusions; one confirmed acceptable results with a false-negative rate of 7%, and the other showed an unacceptably high false-negative rate of 46.4%. The Sentinel Node Oriented Tailored Approach （SENORITA） trial is a multicenter randomized controlled phase 1/I trial being performed in Korea. Patient enrollment is now complete and the long-term results are currently awaited. Recently, an image-guided SN mapping technique using infrared ray/fluorescence was introduced. This method might be a promising technology because it allows the clear visualization of SNs. With regard to the primary tumor, the non-exposed endoscopic wall-inversion surgery technique and non-exposure endolaparoscopic full-thickness resection with simple suturing technique have been reported. These methods prevent abdominal infection and tumor seeding and can be good alternatives to conventional laparoscopic gastric wedge resection. For indications, SN navigation surgery can be extended to patients who underwent non-curative endoscopic resection. Although a few studies have been performed on these patients, sentinel concepts may be beneficial to patients as they omit the need for additional gastrectomy. SN navigation surgery can lead to actual organ-preserving surgery and plays a key role in improving the quality of life of patients with early gastric cancer in the future.

关键词： Sentinel node navigation surgery early gastric cancer SENORITA

Interactions between Transmembrane Helices within Monomers of the Aquaporin AtPIP2;1 Play a Crucial Role in Tetramer Formation

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Molecular Plant 2016年第7期9卷 1004-1017页

作者： Yun-Joo Yoo Hyun Kyung Lee Wonhee Han Dae Heon Kim Myoung Hui Lee Jouhyun Jeon Dong Wook Lee Junho Lee Yongjik Lee Juhun Lee Jin seok Kim Yunje cho Jin-Kwan Han Inhwan Hwang School of Interdisciplinary Bioscience and Bioengineering Pohang University of Science and Technology Pohang 790-784 Korea Department of Life Sciences Pohang University of Science and Technology Pohang 790-784 Korea Department of Biology Sunchon National University Sunchon 57922 Korea Division of Integrative Biosciences and Biotechnology Pohang University of Science and Technology Pohang 790-784 Korea

Aquaporin （AQP） is a water channel protein found in various subcellular membranes of both prokaryotic and eukaryotic cells. The physiological functions of AQPs have been elucidated in many organisms. However, understanding their biogenesis remains elusive, particularly regarding how they assemble into tetramers. Here, we investigated the amino acid residues involved in the tetramer formation of the Arabidopsis plasma membrane AQP AtPIP2;1 using extensive amino acid substitution mutagenesis. The mutant proteins V41A/ E44A, F51A/L52A, F87A/191A, F92A/193A, V95A/Y96A, and H216A/L217A, harboring alanine substitutions in the transmembrane （TM） helices of AtPIP2;1 polymerized into multiple oligomeric complexes with a vari- able number of subunits greater than four. Moreover, these mutant proteins failed to traffic to the plasma membrane, instead of accumulating in the endoplasmic reticulum （ER）. Structure-based modeling revealed that these residues are largely involved in interactions between TM helices within monomers. These results suggest that inter-TM interactions occurring both within and between monomers play crucial roles in tetramer formation in the AtPIP2;1 complex. Moreover, the assembly of AtPIP2;1 tetramers is critical for their trafficking from the ER to the plasma membrane, as well as water permeability.

关键词： aquaporin AtPIP2 tetramer formation interaction between transmembrane helices

Light Triggers the miRNA-Biogenetic Inconsistency for De-etiolated Seedling Survivability in Arabidopsis thaliana

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Molecular Plant 2020年第3期13卷 431-445页

作者： Suk Won choi Moon Young Ryu Andras Viczian Hyun Ju Jung Gu Min Kim Agustin LArce Natalia PAchkar Pablo Manavella Ulla Dolde Stephan Wenke Attila Molnar Ferenc Nagy seok Keun cho Seong Wook Yang Department of Systems Biology Institute of Life Science and BiotechnologyYonsei UniversitySeoul 120-749Korea Institute of Plant Biology Biological Research Centre(BRC)Hungarian Academy of SciencesTemesvari krt.62Szeged 6726Hungary Instituto de Agrobiotecnologia del Litoral(IAL)Centro Cientifico Tecnologico Santa Fe(CCT) Santa FeArgentina Laboratoire de Recherche en Sciences Vegetates 24chemin de Borde-RougeBP 42617 AuzevilleCastanet-Tolosan 31326France Department of Plant and Environmental Sciences Faculty of ScienceUniversity of CopenhagenThorvaldsensvej 40FrederiksbergCopenhagen 1871Denmark Institute of Molecular Plant Sciences School of Biological SciencesThe King’s BuildingsUniversity of EdinburghEdinburgh EH93BFUK These authors contributed equally to this article

The shift of dark-grown seedlings into light causes enormous transcriptome changes followed by a dramatic developmental ***,we show that microRNA(miRNA)biogenesis also undergoes regulatory changes during *** seedlings maintain low levels of primary miRNAs(pri-miRNAs)and miRNA processing core proteins,such as Dicer-like 1,SERRATE,and HYPONASTIC LEAVES 1,whereas during de-etiolation both pri-miRNAs and the processing components accumulate to high ***,the levels of most miRNAs do not notably increase in response to *** reconcile this inconsistency,we demonstrated that an unknown suppressor decreases miRNA-processing activity and light-induced SMALL RNA DEGRADING NUCLEASE 1 shortens the half-life of several miRNAs in de-etiolated *** together,these data suggest a novel mechanism,miRNA-biogenetic inconsistency,which accounts for the intricacy of miRNA biogenesis during *** mechanism is essential for the survival of de-etiolated seedlings after long-term skotomorphogenesis and their optimal adaptation to ever-changing light conditions.

关键词： Light signaling miRNA biogenesis

Fe_2O_3对粉青裂纹釉的制备及性能影响

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中国陶瓷 2019年第1期55卷 64-69页

作者：董伟霞包启富 cho Woo seok 景德镇陶瓷大学材料与工程学院景德镇333001 Icheon Branch Korea Institute of Ceramic Engineering and Technology

主要利用韩国青瓷坯体在1270～1290℃还原气氛下制备粉青裂纹釉。考察了不同坯体种类、釉用氧化铁含量对粉青裂纹釉的影响。研究结果表明:合适的坯体应为收缩小,铁含量为1%～2%的瓷质浅灰色坯体有利于粉青釉的呈色和玉质感。随着氧化铁... 详细信息

主要利用韩国青瓷坯体在1270～1290℃还原气氛下制备粉青裂纹釉。考察了不同坯体种类、釉用氧化铁含量对粉青裂纹釉的影响。研究结果表明:合适的坯体应为收缩小,铁含量为1%～2%的瓷质浅灰色坯体有利于粉青釉的呈色和玉质感。随着氧化铁含量的增加,釉面由白色、粉青色、绿色最后逐渐向黄色色调过渡,并且釉面的裂纹逐渐由稠密向稀疏直裂纹转化。当氧化铁含量为0.020 mol时,由于釉面吸收了红光而反射出蓝绿光,因此釉面呈现粉青色(L*=65.18, a*=-6.67, b*=1.63)。从XRD和SEM结果可知:韩国青瓷坯体结构致密,釉面中存在很多细小结构的微气泡和未被溶解的石英相对光造成漫发射,从而赋予了釉面玉质感。

关键词：韩国青瓷坯体仿龙泉青瓷粉青裂纹釉氧化铁

Efficient and power-awareness query over sensor networks

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重庆邮电大学学报（自然科学版） 2007年第3期19卷 270-275页

作者： Sang-Hun Eo Ho-seok Kim Sook-Kyoung cho Hae-Young Bae Dept. of Computer Science & Information Engineering Inha University

Sensor networks consisted of low-cost, low-power, multifunctional miniature sensor devices have played an important role in our daily life. Light and humidity monitoring, seismic and animal activity detection, environment and habitat monitoring are the most common applications. However, due to the limited power supply, ordinary query methods and algorithms can not be applied on sensor networks. Queries over sensor networks should be power-aware to guarantee the maximum power savings. The minimal power consumption by avoiding the expensive communication of the redundant sensor nodes is concentrated on. A lot of work have been done to reduce the participated nodes, but none of them have considered the overlapping minimum bounded rectangle (MBR) of sensors which make them impossible to reach the optimization solution. The proposed OMSI-tree and OMR algorithm can efficiently solve this problem by executing a given query only on the sensors involved. Experiments show that there is an obvious improvement compared with TinyDB and other spatial index, adopting the proposed schema and algorithm.

关键词：传感器网络查询最小界矩形效率

Multi-objective Optimization Based on Unsteady Analysis Considering the Efficiency and Radial Force of a Single-Channel Pump for Wastewater Treatment

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Journal of Mechanics Engineering and Automation 2016年第5期6卷 234-245页

作者： Jin-Hyuk Kim Bo-Min cho Yotmg-seok choi Kyotmg-Yong Lee Thermal & Fluid System R&D Group Korea Institute of lndustrial Technology Cheonan Republic of Korea Advanced Energy and Technology University of Science and Technology Daejeon Republic of Korea