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Physiological differences in the growth and maturation of Eisenia bicyclis and Ecklonia cava gametophytes in Korea

Journal of Oceanology and Limnology 2019年第2期37卷 657-664页

作者： Han Gil CHOI Da Vine JEON seo kyoung park GAO Xu Faculty of Biological Science and Sea & Biotech Wonkwang University

The objective of this study was to examine the eff ects of temperature and light intensity on growth of female gametophytes of Eisenia bicyclis and Ecklonia cava and responses of these female gametophytes to Fe addition and daylength. Female gametophytes of each species were cultured at four temperatures (10, 15, 20, and 25°C) and under a combination of four light intensities (10, 20, 40, and 80 μmol photons/(m^2 ·s)) with two temperatures (15 and 20°C for E i . bicyclis;20 and 25°C for E c . cava ) to clarify their optimal growth conditions. Growth and maturation of female gametophytes of these two species under a combination of five Fe-EDTA concentrations (0, 1, 2, 4, and 8 μmol/L) and three daylengths (10, 12, and 14 h) were also examined. The growth of E i . bicyclis gametophyte was maximal at approximately 15–20°C, 20 μmol photons/(m^2 ·s), Fe-EDTA concentration of 8 μmol/L and daylengths of 12–14 h. While E c . c ava gametophytes showed optimal growth at approximately 20–25°C, 20 μmol photons/(m^2 ·s), Fe- EDTA concentration of 8 μmol/L and daylength of 14 h. Maturation of gametophytes was enhanced at Fe-EDTA concentration of 4 μmol/L for E i . bicyclis and at 2 μmol/L for E c . c ava . In conclusion, optimal growth temperatures and Fe-EDTA concentrations for maturation of E i . bicyclis and E c . c ava gametophytes were diff erent. Higher optimal growth temperature for E c . c ava gametophytes may contribute to its wider geographical distribution compared to E i . bicyclis which has restricted habitats in Korea. This suggests that addition of Fe ion could be used to recover beds of these two species in barren grounds of Korea.

关键词： Ecklonia cava Eisenia bicyclis gametophyte growth maturation

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Biomass and Community Structure of Epilithic Biofilm on the Yellow and East Coasts of Korea

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Open Journal of Marine Science 2014年第4期4卷 286-297页

作者： Bo Yeon Kim seo kyoung park Jin Suk Heo Han Gil Choi Young Sik Kim Ki Wan Nam Faculty of Biological Science and Institute of Basic Natural Sciences Wonkwang University Iksan Korea School of Marine Life Science Kunsan National University Kunsan Korea Department of Marine Biology Pukyong National University Busan Korea

Spatial biomass variation and community structure of epilithic biofilms were examined using cell counts, chlorophyll a extraction, and remote-sensing techniques. Samples were collected at two levels of wave exposure along the Yellow and East Coasts of Korea in December 2010. Cyanobacteria were dominant, occupying about 88% of biofilm, irrespective of wave exposure levels. The cyanobacteria species, Aphanotece spp. was abundant in the Yellow Coast location and Lyngbya spp. was abundant in the East coast location. The representative diatoms were Navicula spp. and Achnanthes spp. on the rocky shores of all study sites. Average Normalized Difference Vegetation Index (NDVI) was significantly greater in the Yellow Coast (mean 0.46) than that in the East Coast (mean 0.21);a similar pattern was observed in Vegetation Index (VI). Chlorophyll a content was three times greater on the Yellow Coast (20.50 μg/cm2) than that on the East Coast (8.21 μg/cm2), and it was greater at the Gosapo and Bangpo shore sites than that at the Gyeokpo site, on the Yellow Coast. However, chlorophyll a contents were not different between 23.33 and 17.66 μg/cm2 at exposed- and sheltered-shores of Yellow Coast, and were 9.62 μg/cm2 and 6.80 μg/cm2 on the East Coast. Vegetation indices were positively correlated with chlorophyll a contents. In conclusion, biofilm of Korean upper rocky shore was mainly composed of cyanobacteria and biofilm biomass that differed between the Yellow and East Coast.

关键词： Biomass Community Structure Epilithic Biofilm NDVI and VI

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Subacute electroacupuncture at Baihui (GV 20) and Dazhui (GV 14) promotes post-stroke functional recovery via neurogenesis and astrogliosis in a photothrombotic stroke mouse model

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Journal of Traditional Chinese Medicine 2019年第6期39卷 833-841页

作者： Young-Wook park Gi Yoon Heo Min Jae Kim seo-Yeon Lee Byung Tae Choi Hwa kyoung Shin Division of Meridian and Structural Medicine School of Korean MedicinePusan National UniversityYangsanGyeongnam 50612Republic of Korea Department of Korean Medical Science School of Korean MedicinePusan National UniversityYangsanGyeongnam 50612Republic of Korea Graduate Training Program of Korean Medicine for Healthy-Aging Pusan National UniversityYangsanGyeongnam 50612Republic of Korea Korean Medical Science Research Center for Healthy-Aging Pusan National UniversityYangsanGyeongnam 50612Republic of Korea

OBJECTIVE:To investigate the optimal timing and underlying mechanism of electroacupuncture(EA)at Baihui(GV 20)and Dazhui(GV 14)for improved long-term functional recovery after focal cerebral ischemia in a photothrombotic stroke mouse ***:Totally 50 adult male C57 BL/6 J mice were assigned into 5 groups:(a)the control group,sham-operated mice(n=10);(b)the vehicle group,focal cerebral ischemia induction without EA(n=10);(c)the acute EA group,mice received EA immediately post-ischemia,followed by once-daily treatments for 7 consecutive days(n=10);(d)the subacute EA group,mice received EA 4 days post-ischemia,followed by once-daily treatments for 7 consecutive days(n=10);(e)the delayed EA *** stimulation(2 Hz,2 V for 20 min)was applied to acupuncture points(acupoints),Baihui(GV 20)and Dazhui(GV 14),once a day for 7 consecutive days beginning immediately(acute treatment),4 d(subacute treatment)and 10 d(delayed treatment)after focal cerebral ischemia in C57 BL/6 J *** assessments were conducted 21 and 28 d post-ischemia and histopathological analyses were performed 28 days ***:The subacute EA treatment at Baihui(GV20)and Dazhui(GV 14)significantly improved functional recovery compared to the vehicle group 28 d after ischemic brain injury,although brain atrophy was not *** number of Neu N+and Neu N+/Brd U+cells as well as GFAP intensity in the ipsilateral cortex were significantly increased in the subacute group compared to the vehicle group 28 d *** concluded that EA stimulation 4 d post-ischemia(subacute treatment)enhanced neurogenesis and astrogliosis,likely contributing to long-term functional recovery following focal cerebral ***:Our findings suggest that the timing of the EA therapy at Baihui(GV 20)and Dazhui(GV 14)determines the therapeutic effects in mice with focal cerebral ischemia induced by photothrombotic occlusion.

关键词： Electroacupuncture Stroke Astrocytes Neurogenesis Post-stroke remodeling

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Calcified Cardiac Mass Compressing Coronary Artery

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Chinese Medical Journal 2018年第17期131卷 2128-2129页

作者： kyoung-Woo seo Jin-Sun park Department of Cardiology Ajou University School of Medicine Suwon 16499 Korea

To the Editor： A 49-year-old male was referred for the left calcified cardiac border detected incidentally by routine chest radiography. He had no cardiac symptoms.

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Reliable beacon transmission based MAC protocol for LR-WPANs over WLAN interferences

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Journal of Zhejiang University-Science C(Computers and Electronics) 2014年第6期15卷 470-481页

作者： Ji-Hoon park Byung-seo KIM ITO Business Division Nongshim Data System Department of Computer and Information Communications Engineering Hongik University

The use of IEEE 802.15.4 standard based application systems has been rapidly increasing, for example, in medical services, sensor networks, public safety systems, and home automation systems. However, issues arise from the fact that IEEE 802.15.4 standard based low rate wireless personal area networks(LR-WPANs) use the same frequency bands as wireless local area networks(WLANs), and they interfere with each other. Based on past research on this issue, the interference has a more serious impact on LR-WPANs' performance than on WLANs' performance. In this paper we propose a method to improve LR-WPANs' performance while coexisting with WLANs, which is called the reliable beacon transmission based medium access control(MAC) protocol. Since the reliability of a beacon frame is important, in this method, only the beacon frame is transmitted in interference-free channels, and the data packets are transmitted in interfered channels instead of abandoning the channels altogether. This method increases the reliability of beacon frames as well as overall channel utilizations. The effectiveness of the proposed method was evaluated through extensive simulations, and this paper proves that this method improves the performance of IEEE 802.15.4 based wireless sensor networks(WSNs) over WLANs' interferences.

关键词： Low rate wireless personal area network(LR-WPAN) Interference Wireless local area network(WLAN) Beacon

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Effect of internal sloshing on added resistance of ship

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Journal of Hydrodynamics 2017年第1期29卷 13-26页

作者： Min-Guk seo Yonghwan Kim Dong-Min park Korea Research Institute of Ships and Ocean Engineering DaejeonKorea Department of Naval Architecture and Ocean Engineering Seoul National UniversitySeoulKorea

The motion responses of ships carrying liquid cargo are affected not only by external wave excitation, but also by internal sloshing-induced forces and moments. Sloshing flow is coupled with the ship motion. This means the added resistance in waves may change when sloshing occurs inside the tank of the ship. In this study, the motion responses and added resistance of a ship, coupled with the sloshing-induced internal forces and moments are considered by using the linear potential theory. The three-dimensional Rankine panel method, in which the physical quantities are represented by using B-spline basis function, is applied. The sloshing flow of inner tanks is also simulated by Using the Rankine panel method and linearized boundary value problem. To study the added resistance, a near-field method, which integrates the second-order pressure on a body surface, is applied. The model ship is a blunt modified Wigley model with two inner tanks. Numerical results obtained without inner tanks are compared with the experimental data, and then the effect of filling ratio of inner tanks on ship motion and added resistance are observed. The components that induce added resistance are examined, and the effects of surge motion on sloshing flow and added resistance are briefly considered. This study shows that the sloshing flow inside the inner tanks may significantly influence not only the motion responses, but also added resistance, especially, when the incident wave frequency approaches the resonance frequency of the sloshing flow.

关键词： Added resistance sloshing seakeeping coupled effect Rankine panel method

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Significant control of metal-insulator transition temperature through catalytic excessive oxygen doping in high-performance vanadium dioxide nanobeam channel

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Journal of Materials Science & Technology 2020年第9期44卷 96-101页

作者： Minhwan Ko Sang Yeon Lee Jucheol park Hyungtak seo Department of Energy Systems Research Ajou UniversitySuwon16499Republic of Korea Gyeongbuk Science Technology Promotion Center Gumi Electronics&Information Technology Research InstituteGumi39717Republic of Korea Department of Materials Science and Engineering Ajou UniversitySuwon16499Republic of Korea

The strategy of a reliable transition temperature control of vanadium dioxide(VO2)is *** VO2 nanobeams were synthesized by a thermal chemical vapor deposition(TCVD)*** metal-insulator transition(MIT)temperature increases to above 380K when the TiO2 ratio of the source is 5 at.%,although the Ti source is not physically doped into VO2 *** XPS spectra of the V 2p orbital reveal the excessive oxidation of V after the TCVD processes with a higher TiO2 ratio,indicating that the TiO2 precursor is important in the O-doping of the surface V O bonds when forming volatile Ti-O gas ***,TiO2 reactants can be used as a VO2 surface chemical modifier to manipulate the MIT transition temperature and maintain a homogenous VO2 phase,which is useful for a Mott device application with a record on/off switching ratio>104 and Mott transition temperature>380 K.

关键词： VO2 Mott transition Metal-insulator transition temperature Oxygen doping Titanium catalyst

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Tailored eradication vs empirical bismuth-containing quadruple therapy for first-line Helicobacter pylori eradication: A comparative,open trial

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World Journal of Gastroenterology 2019年第46期25卷 6743-6751页

作者： Youn I Choi Jun Won Chung Dong Kyun park kyoung Oh Kim Kwang An Kwon Yoon Jae Kim Ja Young seo Department of Gastroenterology Gil Medical CenterGachon UniversityIncheon 21565South Korea Department of Laboratory Medicine Gil Medical CenterGachon UniversityIncheon 21565South Korea

BACKGROUND Few studies have compared the efficacy and safety profile of a tailored eradication(TR)strategy based on the presence of a 23S ribosomal RNA point mutation with those of empirical bismuth-based quadruple therapy(EBQT)for first-line eradication of Helicobacter pylori(***)in Korean *** To compare the efficacy and safety of a TR strategy and those of EBQT regimen as first-line eradication therapy for *** This is an open-label,comparative study in which we prospectively enrolled patients over 18 years of age with *** infection and retrospectively reviewed their ***-positive patients diagnosed by rapid urease test,Giemsa staining,or dual priming oligonucleotide polymerase chain reaction(DPO-PCR)were enrolled from May 2016 to September 2018 at Gil Medical *** with *** infection received either a TR regimen or the EBQT *** the tailored therapy group that underwent DPO-PCR testing,patients with A2142G and/or A2143G point mutations were treated with a bismuth-containing quadruple *** eradication rate,patient-reported side effect rate,and *** eradication success rate were evaluated and compared between the *** A total of 150 patients were assigned to the TR(n=50)or EBQT group(n=100).The first-line eradication rate of *** did not differ between the groups(96.0%vs 95.7%,P=0.9).The rate of eradication-related side effects for TR was 12.0%,which differed significantly from that of EBQT(43.0%)for first-line treatment(P<0.001).CONCLUSION DPO-PCR-based TR for *** eradication may be equally efficacious,with less treatment-related complications,compared to EBQT in Korea,where clarithromycin resistance is high.

关键词： Helicobacter pylori Eradication Tailored Empirical Quadruple

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Specific inhibition of Streptococcus bovis by endolysin LyJH307 supplementation shifts the rumen microbiota and metabolic pathways related to carbohydrate metabolism

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Journal of Animal Science and Biotechnology 2022年第1期13卷 209-223页

作者： Hanbeen Kim Tansol park Inhyuk Kwon Jakyeom seo Department of Animal Science Life and Industry Convergence Research InstitutePusan National University1268-50 Samrangjin-roMiryang 50463Republic of Korea Department of Animal Science and Technology Chung-Ang UniversityAnseong-siGyeonggi-do 17546Republic of Korea EASY BIO Inc.Seoul 06253South Korea

Background:Endolysins,the bacteriophage-originated peptidoglycan hydrolases,are a promising replacement for antibiotics due to immediate lytic activity and no antibiotic *** objectives of this study were to investigate the lytic activity of endolysin LyJH307 against *** and to explore changes in rumen fermentation and microbiota in an in vitro *** treatments were used:1)control,corn grain without LyJH307;and 2)LyJH307,corn grain with LyJH307(4 U/mL).An in vitro fermentation experiment was performed using mixture of rumen fluid collected from two cannulated Holstein steers(450±30 kg)and artificial saliva buffer mixed as 1:3 ratio for 12 h incubation *** vitro dry matter digestibility,pH,volatile fatty acids,and lactate concentration were estimated at 12 h,and the gas production was measured at 6,9,and 12 *** rumen bacterial community was analyzed using 16S rRNA amplicon ***:LyJH307 supplementation at 6 h incubation markedly decreased the absolute abundance of ***(approximately 70% compared to control,P=0.0289)and increased ruminal pH(P=0.0335)at the 12 h *** acetate proportion(P=0.0362)was significantly increased after LyJH307 addition,whereas propionate(P=0.0379)was ***307 supplementation increased D-lactate(P=0.0340)without any change in L-lactate concentration(P>0.10).There were no significant differences in Shannon’s index,Simpson’s index,Chao1 estimates,and evenness(P>0.10).Based on Bray-Curtis dissimilarity matrices,the LyJH307 affected the overall shift in microbiota(P=0.097).LyJH307 supplementation induced an increase of 11 genera containing Lachnoclostridium,WCHB1-41,unclassified genus Selenomonadaceae,Paraprevotella,vadinBE97,Ruminococcus gauvreauii group,Lactobacillus,Anaerorhabdus furcosa group,Victivallaceae,Desulfuromonadaceae,and *** predicted functional features represented by the Kyoto Encyclopedia of Genes and Genomes pathways were changed by LyJH307 toward a de

关键词： Endolysin Rumen microbiota Ruminal acidosis Streptococcus bovis

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Submerged arc plasma system combined with ozone oxidation for the treatment of wastewater containing non-degradable organic compounds

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Frontiers of Environmental Science & Engineering 2021年第5期15卷 155-163页

作者： Byungjin Lee Eun seo Jo Dong-Wha park Jinsub Choi Department of Chemistry and Chemical Engineering and Regional Innovation Center for Environmental Technology of Thermal Plasma(RIC-ETTP) INHA UniversityIncheon402-751Republic of Korea

Submerged arc plasma technology was assessed for the removal of phenols from *** OH radicals generated from the boundary between the plasma and waste solution were considered as a significant factor on the degradation *** this study,the effects of highly energetic electrons released from the submerged arc plasma were mainly *** highly energetic electrons directly broke the strong chemical bond and locally increased the reaction temperatures in *** effects of the submerged-arc plasma on the decomposition of phenol are discussed in terms of the input energy and initial *** single use of submerged arc plasma easily decomposed the phenol but did not increase the mineralization ***,the submerged arc plasma,coupled with the ozone injection,was *** submerged arc plasma combined with ozone injection had a synergic effect,which led to significant improvements in mineralization with only a small increase in input *** decomposition mechanism of phenol by the submerged arc plasma with the ozone was analyzed.

关键词： Thermal plasma Submerged arc plasma Wastewater Ozone Phenol Highly energetic electron

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