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Physiological differences in the growth and maturation of Eisenia bicyclis and Ecklonia cava gametophytes in Korea

Journal of Oceanology and Limnology 2019年第2期37卷 657-664页

作者： Han Gil CHOI Da Vine JEON seo kyoung park GAO Xu Faculty of Biological Science and Sea & Biotech Wonkwang University

The objective of this study was to examine the eff ects of temperature and light intensity on growth of female gametophytes of Eisenia bicyclis and Ecklonia cava and responses of these female gametophytes to Fe addition and daylength. Female gametophytes of each species were cultured at four temperatures (10, 15, 20, and 25°C) and under a combination of four light intensities (10, 20, 40, and 80 μmol photons/(m^2 ·s)) with two temperatures (15 and 20°C for E i . bicyclis;20 and 25°C for E c . cava ) to clarify their optimal growth conditions. Growth and maturation of female gametophytes of these two species under a combination of five Fe-EDTA concentrations (0, 1, 2, 4, and 8 μmol/L) and three daylengths (10, 12, and 14 h) were also examined. The growth of E i . bicyclis gametophyte was maximal at approximately 15–20°C, 20 μmol photons/(m^2 ·s), Fe-EDTA concentration of 8 μmol/L and daylengths of 12–14 h. While E c . c ava gametophytes showed optimal growth at approximately 20–25°C, 20 μmol photons/(m^2 ·s), Fe- EDTA concentration of 8 μmol/L and daylength of 14 h. Maturation of gametophytes was enhanced at Fe-EDTA concentration of 4 μmol/L for E i . bicyclis and at 2 μmol/L for E c . c ava . In conclusion, optimal growth temperatures and Fe-EDTA concentrations for maturation of E i . bicyclis and E c . c ava gametophytes were diff erent. Higher optimal growth temperature for E c . c ava gametophytes may contribute to its wider geographical distribution compared to E i . bicyclis which has restricted habitats in Korea. This suggests that addition of Fe ion could be used to recover beds of these two species in barren grounds of Korea.

关键词： Ecklonia cava Eisenia bicyclis gametophyte growth maturation

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Prevalence and risk factors of wound complications after transtibial amputation in patients with diabetic foot

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World Journal of Diabetes 2024年第4期15卷 629-637页

作者： Young Uk park seong Hyuk Eim Young Wook seo Department of Orthopedic Surgery Ajou University School of MedicineAjou University HospitalSuwon 16499South Korea

BACKGROUND Diabetic foot(DMF)complications are common and are increasing in *** factors related to wound complications are yet to be established after transtibial amputation under the diagnosis of DMF *** To analyze the prognosis and risk factors related to wound complications after transtibial amputation in patients with *** This retrospective cohort study included seventy-two patients with DMF complications who underwent transtibial amputation between April 2014 and March *** groups were categorized based on the occurrence of wound complications,and we compared demographic data between the complication group and the non-complication group to analyze risk ***,a multivariate logistic regression analysis was performed to identify risk *** The average follow-up period was 36.2 *** the 72 cases,31(43.1%)had wound *** these,12 cases(16.7%)received further treatment,such as debridement,soft tissue stump revision,and re-amputation at the proximal *** a group that required further management due to wound complications after transtibial amputation,the hemoglobin A1c(HbA1c)level was 9.32,while the other group that did not require any treatment had a 7.54 HbA1c *** prevalence of a history of kidney transplantation with wound complications after transtibial amputation surgery in DMF patients was significantly greater than in cases without wound complications(P=0.02).Other factors did not show significant *** Approximately 43.1%of the patients with transtibial amputation surgery experienced wound complications,and 16.7%required additional surgical *** HbA1c levels and kidney transplant history are risk factors for postoperative wound complications.

关键词： Diabetic foot Transtibial amputation Wound complications Risk factor

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Biomass and Community Structure of Epilithic Biofilm on the Yellow and East Coasts of Korea

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Open Journal of Marine Science 2014年第4期4卷 286-297页

作者： Bo Yeon Kim seo kyoung park Jin Suk Heo Han Gil Choi Young Sik Kim Ki Wan Nam Faculty of Biological Science and Institute of Basic Natural Sciences Wonkwang University Iksan Korea School of Marine Life Science Kunsan National University Kunsan Korea Department of Marine Biology Pukyong National University Busan Korea

Spatial biomass variation and community structure of epilithic biofilms were examined using cell counts, chlorophyll a extraction, and remote-sensing techniques. Samples were collected at two levels of wave exposure along the Yellow and East Coasts of Korea in December 2010. Cyanobacteria were dominant, occupying about 88% of biofilm, irrespective of wave exposure levels. The cyanobacteria species, Aphanotece spp. was abundant in the Yellow Coast location and Lyngbya spp. was abundant in the East coast location. The representative diatoms were Navicula spp. and Achnanthes spp. on the rocky shores of all study sites. Average Normalized Difference Vegetation Index (NDVI) was significantly greater in the Yellow Coast (mean 0.46) than that in the East Coast (mean 0.21);a similar pattern was observed in Vegetation Index (VI). Chlorophyll a content was three times greater on the Yellow Coast (20.50 μg/cm2) than that on the East Coast (8.21 μg/cm2), and it was greater at the Gosapo and Bangpo shore sites than that at the Gyeokpo site, on the Yellow Coast. However, chlorophyll a contents were not different between 23.33 and 17.66 μg/cm2 at exposed- and sheltered-shores of Yellow Coast, and were 9.62 μg/cm2 and 6.80 μg/cm2 on the East Coast. Vegetation indices were positively correlated with chlorophyll a contents. In conclusion, biofilm of Korean upper rocky shore was mainly composed of cyanobacteria and biofilm biomass that differed between the Yellow and East Coast.

关键词： Biomass Community Structure Epilithic Biofilm NDVI and VI

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Changes in microorganisms and metabolites during long-term fermentation of commercially manufactured anchovy sauce

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Food Science and Human Wellness 2024年

作者： Daehyeon Kim seong-Eun park Eun-Ju Kim Seung-Ho seo Kwang-Moon Cho Sun Jae Kwon Hong-seok Son Department of Biotechnology College of Life Sciences and Biotechnology Korea University Sonlab Inc. 3. AccuGene Inc.

This study was performed to investigate the changes in microbial communities and metabolites during the long-term fermentation of commercially manufactured anchovy sauce. Samples of commercial anchovy sauce were collected from large-scale fermentation tanks with fermentation periods of up to 7.87 years. The complex bacterial community was simplified to two genera， Tetragenococcus and Halanaerobium， after approximately 0.55 years of fermentation. Although genera， such as Saccharomyces， Cladosporium， Candida， and Aspergillus， were relatively dominant， no clear pattern was identified in fungal community analysis. The longitudinal metabolite profile demonstrated that approximately half (55.8%) of the metabolites present in anchovy sauce were produced within a year of fermentation due to rapid fermentation. Despite the static microbial community， the contents of several metabolites including amino acids and biogenic amines changed continuously during the long-term fermentation of anchovy sauce. This study provides novel insights into the changes in microbiota and metabolites in fish sauce produced without any starter inoculation.

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RUFY4 deletion prevents pathological bone loss by blocking endo-lysosomal trafficking of osteoclasts

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Bone Research 2024年第2期12卷 407-420页

作者： Minhee Kim Jin Hee park Miyeon Go Nawon Lee Jeongin seo Hana Lee Doyong Kim Hyunil Ha Taesoo Kim Myeong seon Jeong Suree Kim Taesoo Kim Han Sung Kim Dongmin Kang Hyunbo Shim Soo Young Lee Department of Life Science Ewha Womans UniversitySeoul 03760South Korea The Research Center for Cellular Homeostasis Ewha Womans UniversitySeoul 03760South Korea Department of Biomedical Engineering Yonsei UniversityWonju 26493South Korea KM Convergence Research Division Korea Institute of Oriental MedicineDaejeon 34054South Korea Chuncheon Center Korea Basic Science InstituteChuncheon 24341South Korea Fluorescence Core Imaging Center and Bioimaging Data Curation Center Ewha Womans UniversitySeoul 03760South Korea Multitasking Macrophage Research Center Ewha Womans UniversitySeoul 03760South Korea

Mature osteoclasts degrade bone matrix by exocytosis of active proteases from secretory lysosomes through a ruffled ***,the molecular mechanisms underlying lysosomal trafficking and secretion in osteoclasts remain largely ***,we show with GeneChip analysis that RUN and FYVE domain-containing protein 4(RUFY4)is strongly upregulated during *** lacking Rufy4 exhibited a high trabecular bone mass phenotype with abnormalities in osteoclast function in ***,deleting Rufy4 did not affect osteoclast differentiation,but inhibited bone-resorbing activity due to disruption in the acidic maturation of secondary lysosomes,their trafficking to the membrane,and their secretion of cathepsin K into the extracellular ***,RUFY4 promotes late endosome-lysosome fusion by acting as an adaptor protein between Rab7 on late endosomes and LAMP2 on primary ***,Rufy4-deficient mice were highly protected from lipopolysaccharide-and ovariectomy-induced bone ***,RUFY4 plays as a new regulator in osteoclast activity by mediating endo-lysosomal trafficking and have a potential to be specific target for therapies against bone-loss diseases such as osteoporosis.

关键词： osteoclast inhibited traffic

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Survival benefit of concurrent chemoradiotherapy for advanced ampulla of Vater cancer

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World Journal of Clinical Cases 2024年第2期12卷 267-275页

作者： Chae Hwa Kwon Hyung Il seo Dong Uk Kim Sung Yong Han Suk Kim Nam Kyung Lee Seung Baek Hong Ji Hyun Ahn Young Mok park Byeong Gwan Noh BioMedical Research Institute Pusan National University HospitalBusan 49241South Korea Department of Surgery Pusan National University School of Medicine and Biomedical Research InstitutePusan National University HospitalBusan 49241South Korea Department of Internal Medicine Pusan National University School of Medicine and Biomedical Research InstitutePusan National University HospitalBusan 49241South Korea Department of Radiology Pusan National University School of Medicine and Biomedical Research InstitutePusan National University HospitalBusan 49241South Korea Department of Pathology and Biomedical Research Institute Pusan National University HospitalBusan 49241South Korea

BACKGROUND Currently,there is no standard adjuvant therapy for patients with resected ampulla of Vater(AoV)*** To evaluate the effectiveness of adjuvant concurrent chemoradiotherapy(CCRT)in patients with advanced AoV cancer who underwent curative *** This single-centered,retrospective study included 29 patients with advanced AoV cancer who underwent pancreaticoduodenectomy between 2006 and *** impact of CCRT on advanced AoV cancer was *** The 1-,3-,and 5-yr recurrence-free survival(RFS)rates for patients with advanced AoV cancer were 82.8%,48.3%,and 40.8%,respectively,and the overall survival(OS)rates were 89.7%,62.1%,and 51.7%,***-cular invasion was found to be a significant risk factor for RFS and OS in patients with advanced AoV cancer in the univariate analysis,whereas T stage and lymph node metastasis were significantly associated with OS in the multivariate *** to the patients who did not receive adjuvant CCRT,those who received adjuvant CCRT did not show statistically significant improvements in the RFS and OS,although they had a significantly lower average age and significantly higher platelet-to-lymphocyte *** Adjuvant CCRT did not improve survival outcomes in patients with advanced AoV *** findings contribute to existing knowledge on the effectiveness of CCRT in this patient population and provide important insights for clinical decision-making.

关键词： Advanced ampulla of Vater cancer Adjuvant concurrent chemoradiotherapy Recurrence Survival Vater cancer

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Polymer donors with hydrophilic side-chains enabling efficient and thermally-stable polymer solar cells by non-halogenated solvent processing

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Nano Research Energy 2024年第1期3卷 19-29页

作者： Soodeok seo Jun-Young park Jin Su park Seungjin Lee Do-Yeong Choi Yun-Hi Kim Bumjoon J.Kim Department of Chemical and Biomolecular Engineering Korea Advanced Institute of Science and Technology(KAIST)Daejeon 34141Republic of Korea Department of Materials Engineering and Convergence Technology and ERI Gyeongsang National UniversityJinju 52828Republic of Korea Advanced Energy Materials Research Center Korea Research Institute of Chemical Technology(KRICT)Daejeon 34114Republic of Korea Department of Chemistry and ERI Gyeongsang National UniversityJinju 52828Republic of Korea

Polymer solar cells(PSCs)with high power conversion efficiency(PCE)and environment-friendly fabrication are the main requirements enabling their production in industrial *** the use of non-halogenated solvent processing is inevitable for the PSC fabrication,it significantly reduces the processability of polymer donors(PDS)and small-molecule acceptors(SMAs).This often results in unoptimized blend morphology and limits the device *** address this issue,hydrophilic oligoethylene glycol(OEG)side-chains are introduced into a PD(2EG)to enhance the molecular compatibility between the PD and L8-BO *** 2EG PD induces higher crystallinity and alleviates phase separation with the SMA compared to the reference PD(PM7)with hydrocarbon ***,the 2EG-based PSCs exhibit a higher PCE(15.8%)than the PM7-based PSCs(PCE=14.4%)in the ortho-xylene based ***,benefitted from the reduced phase separation and increased crystallinity of 2EG PDS,the 2EG-based PSCs show enhanced thermal stability(84%of initial PCE after 120 h heating)compared to that of the PM7-based PSCs(60%of initial PCE after 120 h heating).This study demonstrates the potential of OEG side-chain-incorporated materials in developing efficient,stable,and eco-friendly PSCs.

关键词： polymer solar cell polymer donor OEG side-chain non-halogenated solvent process side-chain engineering

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Effect of transcranial direct current stimulation on supernumerary phantom limb pain in spinal cord injured patient:A case report

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World Journal of Clinical Cases 2024年第17期12卷 3177-3182页

作者： Hyo-Sik park Jae-Hyung Kim Department of Interventional Physiatry Eulji University Medical CenterSeo-GuDaejeonSouth Korea

BACKGROUND Supernumerary phantom limb(SPL)sensation is the experience of additional limbs,either single or a pair of *** to traumatic spinal cord injuries,we report effect of transcranial direct current stimulation(tDCS)on SPL pain in a patient with cervical cord *** SUMMARY The subject was a 57-year-old man who was diagnosed with complete spinal cord injury(C6/C5,motor level;C5/C5,sensory level;AIS-A)approximately three months *** a period of 2 wk,we administered anodal tDCS over the motor cortex for 15 minutes at an intensity of 1.5 *** that treatment,the patient experienced a decrease of SPL pain intensity and frequency,which lasted for 1 week after the end of *** Targeting the motor cortex through neuromodulation appears to be a promising option for the management of SPL pain.

关键词： Supernumerary phantom limb pain Spinal cord injury transcranial Direct Current Stimulation Neuromodulation Case report

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Specific inhibition of Streptococcus bovis by endolysin LyJH307 supplementation shifts the rumen microbiota and metabolic pathways related to carbohydrate metabolism

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Journal of Animal Science and Biotechnology 2022年第1期13卷 209-223页

作者： Hanbeen Kim Tansol park Inhyuk Kwon Jakyeom seo Department of Animal Science Life and Industry Convergence Research InstitutePusan National University1268-50 Samrangjin-roMiryang 50463Republic of Korea Department of Animal Science and Technology Chung-Ang UniversityAnseong-siGyeonggi-do 17546Republic of Korea EASY BIO Inc.Seoul 06253South Korea

Background:Endolysins,the bacteriophage-originated peptidoglycan hydrolases,are a promising replacement for antibiotics due to immediate lytic activity and no antibiotic *** objectives of this study were to investigate the lytic activity of endolysin LyJH307 against *** and to explore changes in rumen fermentation and microbiota in an in vitro *** treatments were used:1)control,corn grain without LyJH307;and 2)LyJH307,corn grain with LyJH307(4 U/mL).An in vitro fermentation experiment was performed using mixture of rumen fluid collected from two cannulated Holstein steers(450±30 kg)and artificial saliva buffer mixed as 1:3 ratio for 12 h incubation *** vitro dry matter digestibility,pH,volatile fatty acids,and lactate concentration were estimated at 12 h,and the gas production was measured at 6,9,and 12 *** rumen bacterial community was analyzed using 16S rRNA amplicon ***:LyJH307 supplementation at 6 h incubation markedly decreased the absolute abundance of ***(approximately 70% compared to control,P=0.0289)and increased ruminal pH(P=0.0335)at the 12 h *** acetate proportion(P=0.0362)was significantly increased after LyJH307 addition,whereas propionate(P=0.0379)was ***307 supplementation increased D-lactate(P=0.0340)without any change in L-lactate concentration(P>0.10).There were no significant differences in Shannon’s index,Simpson’s index,Chao1 estimates,and evenness(P>0.10).Based on Bray-Curtis dissimilarity matrices,the LyJH307 affected the overall shift in microbiota(P=0.097).LyJH307 supplementation induced an increase of 11 genera containing Lachnoclostridium,WCHB1-41,unclassified genus Selenomonadaceae,Paraprevotella,vadinBE97,Ruminococcus gauvreauii group,Lactobacillus,Anaerorhabdus furcosa group,Victivallaceae,Desulfuromonadaceae,and *** predicted functional features represented by the Kyoto Encyclopedia of Genes and Genomes pathways were changed by LyJH307 toward a de

关键词： Endolysin Rumen microbiota Ruminal acidosis Streptococcus bovis

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Deep Drawing Process Using a Tractrix Die for Manufacturing Liners for a CNG High-Pressure Vessel(Type Ⅱ)

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Chinese Journal of Mechanical Engineering 2022年第1期35卷 132-143页

作者： Gun‑Young park Hyo‑seo Kwak Hyo‑seong Jang Chul Kim School of Mechanical Engineering Pusan National UniversityPusan 46241South Korea Department of Mechanical Engineering Dong-eui UniversityPusan 47340Korea.

The liner of a CNG pressure vessel is manufactured by a DDI(deep drawing and ironing)process for the cylinder part,which is a continuous process that includes a drawing process to reduce the diameter of the billet and a subsequent ironing process to reduce the thickness of the billet.A tractrix die used in the 1^(st) deep drawing allows the blank to flow smoothly by decreasing the punch load and radial tensile stress occurring in the *** also increases the draw ratio compared to conventional dies,but it causes forming *** this study,a shape coefficient(S_(c))is proposed for the tractrix die using the blank diameter(D_(0)),inflow diameter of the workpiece(d_(i)),and inflow angle of the workpiece(θ)for design of the tractrix *** effects of the thickness and inflow angle of the workpiece on wrinkling and folding were investigated through ***,a discriminant is proposed for the relative radial stress(σ)generated during the deep drawing process using the tractirx die and used to predict *** on the results,the blank thickness,the draw ratio,and the inflow of the workpiece angle in the 1^(st) deep drawing process are suggested,and the number of operations in the DDI process was reduced from 6 to *** improves the productivity and reduces the manufacturing cost.

关键词： Tractrix die Deep drawing Wrinkling Folding LDR(Limit draw ratio) FEM

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