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亚甲基四氢叶酸还原酶基因C677T位点多态性与结直肠癌的相关性分析

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消化肿瘤杂志（电子版） 2024年第3期16卷 306-311页

作者：洪文光陈森明蔡泽华李泓陈健松刘万珍潮州市人民医院胃肠外科广东潮州521011

目的探索亚甲基四氢叶酸还原酶(methylenetetrahydrofolate reductase,MTHFR)基因C677T位点的多态性与结直肠癌(colorectal cancer,CRC)患病风险之间的关系。方法选取2022年1月至2023年12月在潮州市人民医院确诊的101例CRC患者为研究... 详细信息

目的探索亚甲基四氢叶酸还原酶(methylenetetrahydrofolate reductase,MTHFR)基因C677T位点的多态性与结直肠癌(colorectal cancer,CRC)患病风险之间的关系。方法选取2022年1月至2023年12月在潮州市人民医院确诊的101例CRC患者为研究组,另选取同期98例健康人员为对照组。采用聚合酶链反应和Sanger测序,检测受试者MTHFR基因C677T位点多态性。对两组受试者的年龄、性别、体重指数、吸烟史、饮酒史、家族史、基因型等进行比较。基于多因素Logistic回归模型计算调整优势比(adjusted odds ratio,A OR)和95%CI,分析CRC患病的风险因素。结果研究组和对照组MTHFR C677T位点基因型和等位基因分布差异有统计学意义(均P<0.05)。多因素Logistic回归模型分析结果显示,CC、CT基因型的CRC患病风险较TT基因型高(AOR=4.068,95%CI1.672~9.896,P<0.05;AOR=2.866,95%CI 1.481~5.546,P<0.05),C等位基因纯合子(CC)携带者的CRC患病风险较T等位基因(CT+TT)携带者高(AOR=3.176,95%CI 1.718~5.870,P<0.05)。结论MTHFR基因C677T位点CC基因型和C等位基因会增加CRC患病风险。

关键词：结直肠癌亚甲基四氢叶酸还原酶基因多态性

Screening biomarkers for spinal cord injury using weighted gene co-expression network analysis and machine learning

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Neural Regeneration Research 2024年第12期19卷 2723-2734页

作者： Xiaolu Li Ye Yang senming Xu Yuchang Gui Jianmin chen Jianwen Xu Department of Rehabilitation Medicine The First Affiliated Hospital of Guangxi Medical UniversityNanningGuangxi Zhuang Autonomous RegionChina Department of Rehabilitation Medicine Guilin People’s HospitalGuilinGuangxi Zhuang Autonomous RegionChina Department of Rehabilitation Medicine The First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian ProvinceChina

Immune changes and inflammatory responses have been identified as central events in the pathological process of spinal co rd *** can greatly affect nerve regeneration and functional ***,there is still limited understanding of the peripheral immune inflammato ry response in spinal cord inju *** this *** obtained microRNA expression profiles from the peripheral blood of patients with spinal co rd injury using high-throughput *** also obtained the mRNA expression profile of spinal cord injury patients from the Gene Expression Omnibus(GEO)database(GSE151371).We identified 54 differentially expressed microRNAs and 1656 diffe rentially expressed genes using bioinformatics *** enrichment analysis revealed that various common immune and inflammation-related signaling pathways,such as neutrophil extracellular trap formation pathway,T cell receptor signaling pathway,and nuclear factor-κB signal pathway,we re abnormally activated or inhibited in spinal cord inju ry patient *** applied an integrated strategy that combines weighted gene co-expression network analysis,LASSO logistic regression,and SVM-RFE algorithm and identified three biomarke rs associated with spinal cord injury:ANO10,BST1,and *** verified the expression levels and diagnostic perfo rmance of these three genes in the original training dataset and clinical samples through the receiver operating characteristic *** polymerase chain reaction results showed that ANO20 and BST1 mRNA levels were increased and ZFP36L2 mRNA was decreased in the peripheral blood of spinal cord injury *** also constructed a small RNA-mRNA interaction network using ***,we evaluated the proportion of 22 types of immune cells in the peripheral blood of spinal co rd injury patients using the CIBERSORT *** proportions of naive B cells,plasma cells,monocytes,and neutrophils were increased while the proportions of memory B cells,C

关键词： bioinformatics analysis biomarker CIBERSORT GEO dataset LASSO miRNA-mRNA network RNA sequencing spinal cord injury SVM-RFE weighted gene co-expression network analysis

XRCC1基因多态性与食管鳞癌放疗敏感相关性分析

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消化肿瘤杂志（电子版） 2021年第1期13卷 29-34页

作者：陈森明罗招云陈楚芝章耀鸿汕头大学附属潮州市人民医院肿瘤科广东潮州521000 南方医科大学附属潮州市中心医院中心实验室广东潮州521000

目的了解食管鳞癌患者的X射线交错互补修复基因1(X-ray repair cross complementing group1,XRCC1)的4个单核苷酸多态性(single nucleotide polymorphisms,SNP)位点的分布情况及其与放疗敏感性的关系。方法提取175例食管鳞癌患者外周血D... 详细信息

目的了解食管鳞癌患者的X射线交错互补修复基因1(X-ray repair cross complementing group1,XRCC1)的4个单核苷酸多态性(single nucleotide polymorphisms,SNP)位点的分布情况及其与放疗敏感性的关系。方法提取175例食管鳞癌患者外周血DNA,通过特异性引物PCR扩增XR CC1的4个SNP所在基因片段,测序及BLAST多态性分析,并与放疗结果相结合,分析SNP与放疗疗效的相关性。结果175例食管鳞癌患者中有112例能成功扩增并测序XR CC1中4个SNP位点。112例患者全组放疗近期有效率为84.8%,其中,4个SNP位点均未变异的基因型组有效率为74.3%,有1个以上SNP变异的基因型组有效率为89.6%(χ^(2)=4.389,P=0.036);单独第4个SNP位点(G28152 A,Arg399Gln)变异的基因型组和所有含第4个SNP位点变异组有效率分别为91.2%和91.5%,与未变异的基因型组比较差异有统计学意义(χ^(2)=4.014,P=0.045和X2=4.451,P=0.035)。结论XRCC1中SNP变异基因型组与食管鳞癌患者放疗敏感性相关。其中,第4个SNP位点变异的基因型可能是放疗敏感的预测指标。

关键词：食管鳞癌 X射线修复交叉互补基因单核苷酸多态性放射治疗放射敏感性

结合潮汕文化的人文关怀护理在晚期恶性肿瘤中的应用

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消化肿瘤杂志（电子版） 2020年第4期12卷 292-295页

作者：刘万珍邱文纯杨婷陈森明章耀鸿广东省潮州市人民医院肿瘤三科广东潮州521000

目的探讨结合潮汕文化的人文关怀护理在减轻晚期恶性肿瘤患者疼痛,改善生活质量和不良情绪中的应用。方法纳入60例晚期恶性肿瘤患者,将其分为对照组和观察组,每组各30例。对照组采用常规护理方法,观察组采用结合潮汕文化的人文关怀护理... 详细信息

目的探讨结合潮汕文化的人文关怀护理在减轻晚期恶性肿瘤患者疼痛,改善生活质量和不良情绪中的应用。方法纳入60例晚期恶性肿瘤患者,将其分为对照组和观察组,每组各30例。对照组采用常规护理方法,观察组采用结合潮汕文化的人文关怀护理措施,比较两组患者干预后疼痛缓解率、焦虑及抑郁等不良情绪改善情况、生活质量提高情况。结果观察组疼痛缓解率优于对照组(P<0.05),观察组干预后抑郁自评量表(self-rating depression scale,SDS)、焦虑自评量表(self-rating anxiety scale,SAS)评分均高于对照组(P<0.05),观察组SF-36生活质量量表评分高于对照组(P<0.05)。结论结合潮汕文化的人文关怀护理可以减轻晚期恶性肿瘤患者疼痛,改善其焦虑、抑郁等不良情绪,并提高患者的生活质量。

关键词：潮汕文化人文关怀护理恶性肿瘤疼痛不良情绪生活质量

Phytoplankton changes during SE monsoonal period in the Lembeh Strait of North Sulawesi, Indonesia,from 2012 to 2015

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Acta Oceanologica Sinica 2018年第12期37卷 9-17页

作者： TANG senming RACHMAN Arief FITRIA Nurul THOHA Hikmah chen Bin Third Institute of Oceanography Ministry of Natural Resources Research Center for Oceanography Indonesian Institute of Sciences

Phytoplankton species composition and abundance in the Lembeh Strait waters was studied in four cruises of April 2013, May 2014, June 2012 and October 2015, during the period of monsoon transition time of SE *** data obtained the seasonal alternations of phytoplankton community structures and its driving factors were discussed. A total of 416 taxa belonging to 5 classes of phytoplankton were recorded in the four month *** density was averaged 2 348 cell/L and diatoms and dinoflagellates had the most diversified species. Cyanobacterium was characterized by its low species numbers but high abundance in the waters of Lembeh Strait. Total phytoplankton abundance occurred low in April and October in the monsoon transition period and it raised high in May and June during the SE monsoon. Frequently occurred species were pelagic diatoms in addition to cyanobacterium Trichodesmium. Abundance and diversity of phytoplankton significantly differed seasonally. The diatoms Thalassionema and Pseudo-nitzschia, and cyanobacterium Trichodesmium contributed most to the community dissimilarities. Due to potentially higher nutrient supply in the south of Lembeh Strait, diatoms and dinoflagellates showed higher densities in the south than in the north of the ***, cyanobacterium preferred distributing much evenly in all waters, it had higher density in the southern Lembeh Strait. Total phytoplankton abundance is quite low compared with the Jakarta Bay and some bays in China. Analysis showed that nutrients from upwelling forced by SE monsoon are the key factor varying the monthly phytoplankton abundances. Due to its primitive nature state, Lembeh water can be an ideal location for the study of pelagic ecosystem under merely the influence of macro environment changes with lower background noise from human activities.

关键词： Lembeh Strait phytoplankton diversity community alternation Trichodesmium monsoon

创伤性脊髓损伤患者血清和尿液的代谢组学分析

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中国组织工程研究 2024年第32期28卷 5085-5090页

作者：宋佳婷陈建敏王柯文黄蓝莹徐森明桂裕昌许建文广西医科大学第一附属医院康复医学科广西壮族自治区南宁市530021

背景:创伤性脊髓损伤在临床上主要依赖于量表评估与影像学检查,但对于损伤程度的预后评估具有一定局限性,利用代谢组学技术进行生物标志物筛选,对于估计病变范围、损伤与恢复程度以及开发新疗法具有重要意义。目的:使用代谢组学技术来... 详细信息

背景:创伤性脊髓损伤在临床上主要依赖于量表评估与影像学检查,但对于损伤程度的预后评估具有一定局限性,利用代谢组学技术进行生物标志物筛选,对于估计病变范围、损伤与恢复程度以及开发新疗法具有重要意义。目的:使用代谢组学技术来表征创伤性脊髓损伤患者的代谢特征,探寻潜在的生物标志物及失调的代谢途径。方法:收集20例创伤性脊髓损伤患者(观察组)和10例健康受试者(对照组)的血清和尿液样本,进行代谢物分析,然后利用多元变量统计分析进行数据处理,筛选差异代谢物。通过MetaboAnalyst软件进行代谢通路富集,应用logistic回归构建生物标志物组合模型,并分析其与美国脊髓损伤协会(ASIA)分级的关系。结果与结论:两组受试者的血清和尿液中分别检测出160种和73种具有显著差异的代谢物。通路富集分析显示,创伤性脊髓损伤后脂质代谢出现明显的紊乱,包括鞘脂类、亚油酸、α-亚麻酸和花生四烯酸代谢以及糖基磷脂酰肌醇生物合成。识别出他索沙坦和葫芦素糖苷这组生物标志物,并且二者构成的代谢物组合在血清和尿液中的水平与ASIA分级存在相关性。由此可见,代谢组学技术为进一步理解创伤性脊髓损伤病理机制、筛选治疗靶点提供帮助。识别出的代谢生物标志物组合可能为评估创伤性脊髓损伤的严重程度提供参考。

关键词：创伤性脊髓损伤代谢组学生物标志物差异代谢物超高效液相色谱-四极杆飞行时间质谱

乳腺癌改良根治术后大分割放射治疗的近期疗效及不良反应

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中华乳腺病杂志（电子版） 2019年第1期13卷 58-59页

作者：孙健泳章耀鸿陈森明陈冰陈煜宇陈燕芳林文辉广东省潮州市人民医院肿瘤二科 521000

乳腺癌是女性发病率最高的恶性肿瘤[1]。放射治疗作为术后辅助治疗的重要手段起着不可替代的作用。乳腺癌术后辅助放射治疗可以降低局部和区域复发率,并提高患者的长期生存率[2]。近年来,国外多项随机临床研究已证实乳腺癌保留乳房(简... 详细信息

乳腺癌是女性发病率最高的恶性肿瘤[1]。放射治疗作为术后辅助治疗的重要手段起着不可替代的作用。乳腺癌术后辅助放射治疗可以降低局部和区域复发率,并提高患者的长期生存率[2]。近年来,国外多项随机临床研究已证实乳腺癌保留乳房(简称保乳)手术后行大分割放射治疗与常规放射治疗的疗效和不良反应相当[3-4],但对乳腺癌改良根治术后大分割放射治疗的研究相对较少,中国乳腺癌患者接受改良根治术仍占多数,进行大分割放射治疗的研究意义重大,因此,潮州市人民医院开展了乳腺癌改良根治术后大分割放射治疗的前瞻性临床研究,探讨其近期疗效及不良反应。

关键词：乳腺肿瘤改良根治术大分割放射治疗

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NM500钢板冷弯断裂原因分析

物理测试 2024年第4期42卷 53-59页

作者：陈以荟曾智中黎建东王世茹林森明广西盛隆冶金有限公司广西防城港538000

某厂厚度为10 mm的NM500钢板在冷弯试验过程中出现断裂。为研究其断裂原因,通过对弯曲试验断裂钢板与合格的钢板进行冷弯工艺参数、拉伸性能、显微组织及夹杂物类型、断口形貌、能谱检测对比综合分析其冷弯断裂原因。结果表明:钢中大尺... 详细信息

某厂厚度为10 mm的NM500钢板在冷弯试验过程中出现断裂。为研究其断裂原因,通过对弯曲试验断裂钢板与合格的钢板进行冷弯工艺参数、拉伸性能、显微组织及夹杂物类型、断口形貌、能谱检测对比综合分析其冷弯断裂原因。结果表明:钢中大尺寸的MnS和不规则形态、团聚的TiN夹杂物破坏了钢板连续性,在冷弯过程中出现微裂纹,随着应力增大微裂纹扩展,最终导致钢板冷弯断裂。

关键词： NM500 冷弯断裂 MnS和TiN夹杂

Effects of diesel oil on the polychaete Annelida in model benthic ecosystem

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Acta Oceanologica Sinica 1994年第3期13卷 425-431页

作者： Tang senming chen Xiaolin Zhuang Dongfa Wu Shengsan Lin Yu Cai Ziping and Zheng Fengwu(This project was supported by the National Natural Science Foundation of China.(Third Institute of oceanography, State Oceanic Administration,Xiamen 361005, China)

An experiment about the effect of diesel oil pollution on the model benthic ecosystem was conducted inthe land-based tank at the 3rd Institute of Oceanography, State Oceanic Administration, in Xiamen, added with No.0 diesel oil at concentrations of 5, 25, 125, 625 mg/dm3 in water of series sub-tanks for 16 h. The changes in polychaete community were studied in the period of two weeks with results that though all concentrations did not basically altered the population structure and composition, the total biomass decreased significantly and individualstended to be smaller in size. The average weight of individual decreased with the increase of diesel pollutant stress.

关键词： Polychaete benthic ecosystem model experiment diesel oil pollution effect