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Marine Life Science & Technology 2020年第3期2卷 302-308页

作者： Minglei Ma Xinguo Shi senjie lin State Key Laboratory of Marine Environmental Science College of Ocean and Earth SciencesXiamen UniversityXiamen 361102China College of Biological Science and Engineering.Fuzhou University Fuzhou 350108China Department of Marine Sciences University of ConnecticutGrotonCT 06340USA

Rhodopsins are now found in all domains of life,and are classified into two large groups:type II,found in animals and type I found in microbes including Bacteria,Archaea,and *** type II rhodopsin functions in many photodependent signaling processes including vision,type 1 among others contains rhodopsins that function as a light-driven proton pump to convert light into ATP as in proteobacteria(named proteorhodopsin).Proteorhodopsin homologs have been documented in dinoflagellates,but their subcellular localizations and functions are still poorly *** though sequence analyses suggest that it is a membrane protein,experimental evidence that dinoflagellate rhodopsins are localized on the plasma membrane or endomembranes is still *** no robust dinoflagellate gene transformation tool was available,we used HEK 293T cells to construct a mammalian expression system for two dinoflagellate rhodopsin *** success of expressing these genes in the system shows that this mammalian cell type is suitable for expressing dinoflagellate *** of the expressed protein locates these dinoflagellate rhodopsins on the cell *** result indicates that the protein codons and membrane targeting signal of the dinoflagellate genes are compatible with the mammalian cells,and the proteins'subcellular localization is consistent with proton pump rhodopsins.

关键词： Dinoflagellate rhodopsins Proteorhodopsin-Subcellular localization HEK 293T cells

Epiphytic zooplankton community profiles in a typical urban wetland as revealed by DNA metabarcoding

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Journal of Oceanology and Limnology 2024年第5期42卷 1571-1585页

作者： Diwen LIANG Chunrong HUANG senjie lin Jiahua DONG Mingyi LIANG Hailin LUO State Environmental Protection Key Laboratory of Urban Ecological Simulation and Protection South China Institute of Environmental SciencesMinistry of Ecology and Environment of the People’s Republic of ChinaGuangzhou 510530China Department of Marine Sciences University of ConnecticutGrotonConnecticut 06340USA

Zooplankton,a crucial component of urban wetland,are one of the effective bioindicators for monitoring the feeding stocks of organisms at higher trophic levels and assessing the ecological quality of ***,information about the characteristics of epiphytic zooplankton community structure resulted from traditional methods is limited and hindered by the large amount of detritus and sludge attached to the *** investigated the epiphytic zooplankton communities associated with macrophytes(Vallisneria,Nymphaea,and Thalia dealbata)in a subtropical wetland using as DNA markers of the 18 S rRNA gene and the mitochondrial cytochrome c oxidase subunit I(COI)gene.A total of 241 OTUs of zooplankton were obtained from COI amplicons,including 194 OTUs of Rotifera,22 of Cladocera,and 25 of Copepoda,while only 62 OTUs of zooplankton were obtained from 18 S rDNA amplicons including 34 OTUs of Rotifera and 28 of *** zooplankton communities associated with the three macrophytes were similar,but they differed significantly from those in the open ***,there were no significant temporal differences among the zooplankton *** zooplankton communities were dominated by littoral zooplankton such as Testudinella,Lecane,and ***,especially littoral species,utilize macrophytes as food sources and as refuges against *** further led to an increase inαandβdiversity of zooplankton communities in urban *** result suggests that the joint use of multiple molecular markers could improve the taxonomic resolution and generate a comprehensive biodiversity profile of zooplankton.

关键词： environmental DNA metabarcoding diversity macrophyte cytochrome c oxidase subunit I(COI) 18 S rRNA

Development rate of Labidocera euchaeta Giesbrecht in Xiamen Harbor

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Acta Oceanologica Sinica 1990年第3期9卷 439-447页

作者： lin senjie and Li Song Department of Oceanography, Xiamen University, Xiamen, China

-The development rate of Labidocera euchaeta Giesbrecht in the Xiamen Harbor was studied in laboratory. The results showed that the development times of egg and larval stages ( D ) were significantly correlated with temperature ( T ), following the Belehradek function, D = α( T -7. 5)-1.0634. The development time from egg to larval stages and adult, as well as generation time can be predicted from the equation for egg development ( α=411/24) by appropriately multipling the constant (α) with a proportion. The development was not isochronal over the whole larval period. Durations of naupliar stages were short and quasi-isochronal, which favored their non-feeding development. In contrast, durations of copepodid stages (C) were long. CV had the longest duration followed by CI, both of which are considered as the critical stages in the larval development. The acclimation effect of the seasonal temperature on the development rate was also observed.

关键词： Th Lab Development rate of Labidocera euchaeta Giesbrecht in Xiamen Harbor rate

A Rhizobium bacterium and its population dynamics under different culture conditions of its associated toxic dinoflagellate Gambierdiscus balechii

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Marine Life Science & Technology 2021年第4期3卷 542-551页

作者： Zhen Wu Xiaohong Yang senjie lin Wai Hin Lee Paul K.S.Lam State Key Laboratory of Marine Pollution City University of Hong KongHong KongChina Department of Chemistry City University of Hong KongHong KongChina State Key Laboratory of Marine Environmental Science Xiamen UniversityXiamen 361005China Department of Marine Sciences University of ConnecticutGrotonCT 06340USA Department of Biomedical Sciences City University of Hong KongHong KongChina Shenzhen Key Laboratory for the Sustainable Use of Marine Biodiversity Research Centre for the Oceans and Human HealthCity University of Hong Kong Shenzhen Research InstituteShenzhen 518057China

Rhizobium bacteria are known as symbionts of legumes for developing nodules on plant roots and fixing N2 for the host plants but unknown for associations with ***,we detected,isolated,and characterized a Rhizobium species from the marine toxic dinoflagellate Gambierdiscus *** 16S rRNA gene(rDNA)is 99%identical to that of Rhizobium rosettiformans,and the affiliation is supported by the phylogenetic placement of its cell wall hydrolase-encoding gene(cwh).Using quantitative PCR of 16S rDNA and cwh,we found that the abundance of this bacterium increased during the late exponential growth phase of Gambierdiscus and under nitrogen limitation,suggesting potential physiological interactions between the dinoflagellate and the *** is the first report of dinoflagellate-associated Rhizobium bacterium,and its prevalence and ecological roles in dinoflagellate-Rhizobium relationships remain to be investigated in the future.

关键词： Rhizobium Gambierdiscus 16S rRNA gene Cell wall hydrola Edited by Chengchao Chen

ATP utilization mechanism by the harmful algal bloom dinoflagellate Karenia mikimotoi

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ATP utilization mechanism by the harmful algal bloom dinofla...

福建省海洋学会2016年学术年会

作者： Hao Luo Xin lin ling Li lingxiao lin senjie lin State Key Laboratory of Marine Environmental Science Xiamen University Department of Marine Sciences University of Connecticut

Phosphorus is a vital nutrient for marine phytoplankton but its preferred form,dissolved inorganic phosphorus,is often limited in the euphotic ***,the ability to utilize organic phosphorus is believed to confer competitive advantages to dinoflagellates and some other phytoplankton species that form harmful algal ***,the mechanisms regulating the utilization of the diverse organophosphate compounds are still poorly *** this study,we investigated the utilization of adenosine triphosphate(ATP) as well as the molecular mechanism in Karenia mikimotoi,a HAB forming *** were set up in L1 medium as control(+P group),dissolved inorganic phosphorus(DIP)-depleted L1 medium(-P group),and ATP-amended-P medium(ATP group).Differential gene expression was profiled for ATP and +P groups using suppression subtractive hybridization(SSH),454 pyrosequencing,and RT-qPCR *** showed that ATP supported a similar growth rate and cell yield as LI medium that contained 36 μM dissolved inorganic *** observed DIP release from ATP into the medium in the course of the experiment,suggesting that *** cells were expressing extracellular DOP hydrolases,but found that it was not alkaline ***,we confirmed that both the phosphorus and nucleoside components of ATP could be assimilated by *** expression analyses revealed that genes involved in the metabolic pathway of ATP such as phosphate transporter,Canalicular multispecific organic anion transporter 1,and some genes related to purine metabolism were up-regulated in the ATP *** results reveal the general rhythmicity of ATP assimilation,and provide insights into possible target genes and proteins for further research of phosphorus utilization mechanisms in ***.

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面向拟态安全防御的异构功能等价体调度算法

通信学报 2018年第7期39卷 188-198页

作者：刘勤让林森杰顾泽宇国家数字交换系统工程技术研究中心河南郑州450001

拟态安全防御的一个关键环节是异构功能等价体的调度,现有的调度策略缺乏对冗余体间相似度的考虑,且调度算法较为单一。基于此,提出了一种兼顾动态性和可靠性的异构功能等价体调度算法——随机种子最小相似度算法,首先随机确定种子冗余... 详细信息

拟态安全防御的一个关键环节是异构功能等价体的调度,现有的调度策略缺乏对冗余体间相似度的考虑,且调度算法较为单一。基于此,提出了一种兼顾动态性和可靠性的异构功能等价体调度算法——随机种子最小相似度算法,首先随机确定种子冗余体,然后再根据相似度指标选择整体相似度最小的最终调度方案。理论分析和仿真实验表明,该算法的调度周期远高于最长相异性距离算法,而失效率远低于随机调度算法,在动态性和可靠性之间达到了较好的平衡。

关键词：拟态安全防御异构冗余调度相似度随机种子

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南海浮游植物生态学研究进展

厦门大学学报（自然科学版） 2019年第1期58卷 1-10页

作者：李红飞林森杰厦门大学海洋与地球学院近海海洋环境科学国家重点实验室福建厦门361102

作为西太平洋最大的边缘海,南海位于热带-亚热带区域,其水文理化环境复杂,高度多样的浮游植物群落在丰度和生产力上均存在显著差异.迄今为止,人们对该海域浮游植物的时空分布及其影响的认识仍有限.通过收集历史资料,特别是近20年的研究... 详细信息

作为西太平洋最大的边缘海,南海位于热带-亚热带区域,其水文理化环境复杂,高度多样的浮游植物群落在丰度和生产力上均存在显著差异.迄今为止,人们对该海域浮游植物的时空分布及其影响的认识仍有限.通过收集历史资料,特别是近20年的研究成果,从浮游植物时空分布、环境调控因子以及受全球气候变化的影响三方面进行分析,总结南海浮游植物生态学研究的重要进展,进而针对研究现状的不完善之处探讨未来的研究方向,突出有待深入研究的领域,以促使南海浮游植物生态学的研究赶上世界同类海域的研究脚步.

关键词：浮游植物南海群落结构环境调控因子

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厦门港真刺唇角水蚤的生长率

台湾海峡 1990年第1期9卷 48-55页

作者：林森杰李松厦门大学 361005

本文以实验室培养为主,结合自然种群调查,观测厦门港真刺唇角水蚤各发育阶段体长、体重的增长状况,并研究了其生长率与温度、发育期的关系。

关键词：水蚤真剌唇角生长率

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基于安全策略的负载感知动态调度机制

计算机应用 2017年第11期37卷 3304-3310页

作者：顾泽宇张兴明林森杰国家数字交换系统工程技术研究中心郑州450002

针对软件定义网络(SDN)网络控制器流规则篡改攻击等单点脆弱性威胁,传统安全解决方案如备份、容错机制等存在被动防御缺陷,无法从根本上解决控制层安全问题。结合目前移动目标防御、网络空间拟态防御等主动防御技术研究现状,提出一种基... 详细信息

针对软件定义网络(SDN)网络控制器流规则篡改攻击等单点脆弱性威胁,传统安全解决方案如备份、容错机制等存在被动防御缺陷,无法从根本上解决控制层安全问题。结合目前移动目标防御、网络空间拟态防御等主动防御技术研究现状,提出一种基于异构冗余结构的动态安全调度机制。建立控制器执行体与调度体调度模型,根据系统攻击异常、异构度等指标,以安全性为原则设计动态调度策略;同时考虑系统负载因素,通过设计调度算法LASSA将调度问题转化为动态双目标优化问题,以实现优化的调度方案。仿真结果表明,对比静态结构,动态调度机制在累积异常值、输出安全率等指标上有明显优势,说明安全调度机制中的动态性与多样性能够显著提高系统抵御攻击能力,LA-SSA机制负载方差较安全优先调度更平稳,在实现安全调度的同时避免了负载失衡问题,验证了安全调度机制的有效性。

关键词：单点脆弱性主动防御技术动态调度机制安全策略负载感知

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面向拟态防御系统的竞赛式仲裁模型

计算机工程 2018年第4期44卷 193-198页

作者：林森杰刘勤让王孝龙国家数字交换系统工程技术研究中心郑州450001

拟态防御通过构建动态异构冗余的系统架构达到破坏攻击链的目的。为提高仲裁的效率,并进而改进系统的整体执行能力,针对拟态防御系统的仲裁过程,在多数一致性表决的基础上,提出一种竞赛式的仲裁模型,在不改变仲裁余度的前提下增加执行... 详细信息

拟态防御通过构建动态异构冗余的系统架构达到破坏攻击链的目的。为提高仲裁的效率,并进而改进系统的整体执行能力,针对拟态防御系统的仲裁过程,在多数一致性表决的基础上,提出一种竞赛式的仲裁模型,在不改变仲裁余度的前提下增加执行余度。实验结果表明,与三余度拟态系统相比,该模型能够有效地弥补拟态系统的性能损失。

关键词：拟态防御拟态调度器异构冗余仲裁策略仲裁效率竞赛式仲裁模型