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Fractionation of edible bird’s nest glycoprotein hydrolysates:characterisation and antioxidative activities of the fractions

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Food Science and Human Wellness 2022年第4期11卷 886-894页

作者： Poh Kei Chong Sue Lian Mun Lee Sin Chang Abdul Salam Babji seng joe lim Department of Food Sciences Faculty of Science and TechnologyUniversiti Kebangsaan Malaysia43600 UKM BangiSelangorMalaysia Department of Food Science and Nutrition Faculty of Applied SciencesUCSI University Kuala LumpurNo.1Jalan Menara GadingUCSI Heights 56000 CherasKuala LumpurMalaysia Innovation Centre for Confectionery Technology(MANIS) Faculty of Science and TechnologyUniversiti Kebangsaan Malaysia43600 UKM BangiSelangorMalaysia Centre for Innovation and Technology Transfer(INOVASI-UKM) ChancelleryUniversiti Kebangsaan Malaysia43600 UKM BangiSelangorMalaysia

E dible bird’s nest(EBN)hydrolysates have been proven to exhibit enhanced ***,being a macromolecule,fractions with different molecular weights would have different properties and ***,this research was aimed to determine the chemical properties and antioxidant activities of freezedried(EBN_(FD))and spray-dried EBN(EBN_(SD))hydrolysates fractionated using gel permeation chromatography(GPC).Overall,two well-separated fractions were identified(EBN_(fFD1),EBN_(fFD2),EBN_(fSD1) and EBN_(fSD2)).EBNFD demonstrated significantly higher(P≤0.05)peptide(3.6%),total carbohydrate(27.7%)and sialic acid(18.2%)contents than that of *** trend was observed in low molecular weight fractionates(EBN_(fFD2) and EBN_(fSD2)).Meanwhile,the first fractionates(EBN_(fFD1) and EBN_(fSD1))exhibited significantly higher(P≤0.05)hydroxyl radical(·OH)scavenging *** transform infrared(FTIR)spectroscopy demonstrated that all EBN fractionates have similar spectrum,except in the region of N—H(amideⅡ)and C—H alkyl *** conclusion,EBN fractionates with different molecular weights showed different chemical properties and antioxidant activities.

关键词： Fractionates Gel permeation chromatography Hydrolysates Peptide content Sialic acid

Enzymatic recovery of glycopeptides from different industrial grades edible bird’s nest and its by-products:nutrient,probiotic and antioxidant activities,and physicochemical characteristics

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Food Science and Human Wellness 2022年第6期11卷 1555-1564页

作者： Hidayati Syamimi Mohd Noor Rafi dah Mohd Ariff Lee Sin Chang Xin Yi Chai Hui Yan Tan Nur’Aliah Daud Abdul Salam Babji seng joe lim Faculty of Science and Technology Universiti Kebangsaan MalaysiaBangi 43600Malaysia International Institute for Halal Research and Training(INHART) International Islamic University Malaysia(IIUM)Jalan Gombak 53100Malaysia Faculty of Applied Sciences UCSI University Kuala LumpurCheras 56000Malaysia Faculty of Agriculture Science and Technology Universiti Putra Malaysia Bintulu CampusBintulu 97008Malaysia Innovative Centre for Confectionery Technology(MANIS) Faculty of Science and TechnologyUniversiti Kebangsaan MalaysiaBangi 43600Malaysia

This study was conducted to recover edible bird’s nest(EBN)hydrolysates from different grades of EBN,including the industrial by-products,using enzymatic *** nutrient,physicochemical properties and antioxidant activities of the recovered hydrolysates at different hydrolysis times were *** showed that the recovery yield of enzymatic hydrolysis was above 89%for all grades of EBN and the degree of hydrolysis increased over *** content(0.321-0.433 mg/L)was below the permissible tolerance level for all ***,the antioxidant activities(DPPH and ABTS scavenging activities and ferric reducing antioxidant powder(FRAP)activity)were significantly higher(P≤0.05)in hydrolysates recovered from EBN by-products(EBNhC and EBNhD)as compared to the high grade EBN hydrolysates(EBNhA and EBNhB).The in-vitro probiotic activity of EBN and its hydrolysates were examined using the probiotic bacterium Lactobacillus ***,EBN by-products hydrolysate(EBNhD)recorded the highest number of ***(1.1×1011 CFU/mL),indicating that low grade EBN has the potential as prebiotic material that promotes probiotic *** study demonstrated the concept of using EBN by-products hydrolysates for various applications,such as functional ingredients with enhanced bioactivities,to improve its economic value.

关键词： Edible bird’s nest by-products Enzymatic hydrolysis Glycopeptides Lactobacillus plantarum Probiotic activity

3D laparoscopy and fluorescence imaging can improve surgical precision for hepatectomy

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Hepatobiliary Surgery and Nutrition 2024年第3期13卷 544-547页

作者： Joshua Sheng Hao lim Vishal G.Shelat Department of General Surgery Tan Tock Seng HospitalSingaporeSingapore

Laparoscopic hepatectomy for the treatment of hepatocellular carcinoma(HCC)has been shown to have superior short-term and comparable long-term outcomes compared with open hepatectomy in experienced centres(1).Short-term advantages include small incisions,reduced post-operative pain,faster recovery,reduced blood loss and lower risk of complications(2).However,the loss of tactile sensation makes it challenging to accurately locate lesions and define tumour margins.

关键词： 3D laparoscopy hepatocellular carcinoma(HCC) indocyanine green dye(ICG dye) liver resection

Design of Multi-layered Protection Against Guided Mortar Threats Yhrough Numerical Modeling

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Defence Technology（防务技术） 2023年第11期29卷 55-65页

作者： Bonny Thawani seng Kiat lim Laura Brown Richard Critchley Rachael Hazael Cranfield Forensic Institute Cranfield UniversityDefence Academy of the United KingdomShrivenhamSN68LAUK

The trade—off between protection and weight is a constant consideration when designing a portable protective *** mobility is a desirable attribute and protection must therefore adapt,prompting a demand for lightweight,simple to construct,low-cost and effective ballistic protection *** strength and ductility,wave spreading capability and good energy absorption are key properties for ballistic *** materials,polycarbonate,Kevlar?-epoxy,polyurethane foam,and aluminium alloy,possess these properties and were selected for analysis by numerical *** configurations were proven to be an optimal solution,by exploiting the advantages of each material without having large penalties of mass and *** modelling using ANSYS AUTODYN?is used to simulate monolithic and multi-layered target configurations,to obtain the penetration mitigation *** results are analysed to select configurations based on different requirements,such as lowest cost,lowest mass,best performance,and optimal configuration which balanced the three key parameters *** optimal configuration of Aluminium,Kevlar-Epoxy,Polyurethane,and Polycarbonate has layers with thickness of 7,3,38,2 mm respectively with a total mass of 7.97 kg,total cost of$39.86 and penetration of 29.34%(14.67 mm).Polynomial relationships between performance and mass/cost are also determined.

关键词： Hydrocode Fragmentation High velocity Composite structures Foams

Validation of four Helicobacter pylori rapid blood tests in a multi-ethnic Asian population

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World Journal of Gastroenterology 2005年第42期11卷 6681-6683页

作者： Lee-Guan lim Khay-Guan Yeoh Bow Ho seng-Gee lim Department of Gastroenterology and Hepatology National University Hospital 5 Lower Kent Ridge Road Singapore 119074 Republic of Singapore Department of Microbiology National University of Singapore Lower Kent Ridge Road Singapore 119260 Republic of Singapore

AIM: To validate the accuracy of four rapid blood tests in the diagnosis of Helicobacter ***: Consecutive dyspeptic patients scheduled for endoscopy at the National University Hospital,Singapore, were interviewed and had blood drawn for serology. The first 109 patients were tested with BM-test (BM), Pyloriset Screen (PS) and QuickVue (QV), and the next 99 subjects were tested with PS and Unigold (UG).Endoscopies were performed blinded to rapid blood test results and biopsies were taken for culture and rapid urease test. Urea breath tests were performed after endoscopies. The rapid blood test results were compared with four reference tests (rapid urease test, culture,serology, and breath test).RESULTS: The study population composed of 208patients (mean age 43.1 years; range 18-73 years; 119males; 174 Chinese). The number of evaluable patientsfor BM, QV, UG and PS were 102, 102, 95, and 197,respectively. The sensitivity and specificity, respectively were: PS 80.2%, 95.8%; UG 55.9%, 100%; QV 43.3%,100%; BM 67.2%, 97.1%.CONCLUSION: The rapid blood test kits showed high specificity and positive predictive value (97-100%), while sensitivity and negative predictive value ranged widely (43%-80% and 47%-73%, respectively). Among test kits, PS showed the best sensitivity (80%), best negative predictive value (73%) and best negative likelihood ratio (0.207). PS had a specificity of 96%, positive predictive value of 97% and positive likelihood ratio of 19.1.

关键词： Helicobacter pylori Rapid blood test

Dynamic Force Reduction and Heat Transfer Improvement for Horizontal Tubes in Large-Particle Gas-Fluidized Beds

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Journal of Thermal Science 2008年第1期17卷 77-83页

作者： Yusumi Nagahashi John R.Grace Kok-seng lim Yutaka Asako Dept.Mech.Eng. Kochi National College of Technol.KochiJAPAN Professor Dept.Chem.Biological Eng. The University of British ColumbiaVancouverCANADA CSIRO Minerals VictoriaAUSTRALIA Dept.Mech.Eng. Tokyo Metropolitan UniversityTokyoJAPAN

The effects of tube bank configuration on forces and heat transfer were investigated for both two-dimensional and three-dimensional gas fluidized beds. Effective dynamic forces and heat transfer coefficients were measured for several tube bank configurations, and it was found that the average forces are smaller than for a single tube. The heat transfer coefficient can be increased by providing sufficient space for particles to descend around both sides of the tube bank. The results provide useful guidelines for optimizing the configuration of tube banks to achieve high heat transfer coefficients while reducing tube erosion due to dynamic forces.

关键词： fluidized beds forces heat transfer tube arrangement sofid circulation

Economic Assessment of Standby Diesel Generator for Peak Reduction in Commercial and Industrial Buildings:A Case Study in Malaysia

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Journal of Electronic Science and Technology 2017年第4期15卷 400-406页

作者： Kein Huat Chua Yun seng lim Jee Xiong Chew Universiti Tunku Abdul Rahman Cheras 43000 Malaysia

This paper outlines the barriers and potential benefits of using standby diesel generators in mitigating the peak demands for commercial and industrial customers. The feasibility of utilizing the standby diesel generators to reduce the electricity bills for customers is carried out by using the hybrid optimization model for electric renewable(HOMER)software. The size of the standby diesel generator and its operational duration are determined based on the lowest cost of electricity obtained from the evaluations. The economic assessments demonstrate that there is potential to reduce the electricity bills for commercial and industrial customers under the existing fuel price and tariffs. The commercial customers under the tariff C2 have the highest potential to save their electricity bills with the use of standby diesel generators for peak reduction. This study demonstrates the potential of the standby diesel generators in peak reduction.

关键词： Commercial and industrial customers cost of electricity hybrid optimization model for electric renewable(HOMER) peak reduction standby diesel generator

Size prediction of κ-carrageenan droplets formed in co-flowing immiscible liquid

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Particuology 2011年第6期9卷 637-643页

作者： Jun-Yee Leong Tek-Kaun lim Ravindra Pogaku Eng-seng Chan Centre of Materials and Minerals School of Engineering and Information Technology Universiti Malaysia Sabah School of Engineering Monash University

The formation of K-carrageenan droplets in channel emulsification was experimentally investigated. The dispersed phase was vertically injected into co-flowing immiscible palm oil in the direction of gravity. This study focused on predicting K-carrageenan drop size using force balance analysis. The force balance model considers the interracial tension to be the solitary attaching force, while a combination of the drag force from the co-flowing palm oil and the body force of the extruding K-carrageenan liquid act as the detaching forces. The conventional model gave poor predictions for droplet size, with an average relative deviation of 23%. This large deviation could be attributed to necking phenomena and an underestimation of the drag force generated on the shear-thinning K-carrageenan solution. By incorporating correction factors, the average relative deviation of the force balance model dronned to 4%.

关键词： K-Carrageenan Droplet Channel emulsification Co-flowing Dripping Bioencapsulation

Primary care physicians'knowledge,attitude and perception towards homosexuality in Singapore

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Global Health Journal 2021年第4期5卷 209-214页

作者： Derek lim seng Bin Ang Kandang Kerbau Women’s and Children’s Hospital 100 Bukit Timah Road Singapore

Aims:This study aim to gain insights into attitudes,acceptance and knowledge levels among family physicians toward homosexual people,which are important as they may affect care among this at-risk ***:An anonymized self-administered questionnaire in English was posted with the assistance of the College of Family Physicians Singapore in February 2015 to its 1529 members with self-addressed,pre-stamped envelopes for *** questionnaire included demographic questions such as age group,gender,religion,ethnicity,marital status and place of *** and scales that measured attitude,acceptance,knowledge and perception of colleagues who are homosexual were also included in the ***:451 responses(29.5%)were obtained,of which 441 were valid(28.84%).About 52.8%of participants were found to have negative,8.9%neutral and 38.3%positive attitudes toward ***(75.6%)were accepting of homosexuals,with 9.3%neutral and 15.1%not *** levels were poor,with a mean score of 6.9 out of a maximum of 13.A strong correlation was found between the attitudes towards lesbians and gay men(ATLG)and acceptance of homosexuals scales(r=0.69,P<0.001),with moderate correlations between acceptance and knowledge(r=0.54,P<0.001)and ATLG and Knowledge(r=0.54,P<0.001).Conclusion:Despite majority of family physicians having negative attitudes towards homosexuality,most are able to accept *** better education in sexual orientation in the medical educational curriculum,the increase in knowledge will lead to better care for people with homosexuality.

关键词： Attitudes Acceptance Family physician Homosexuality Knowledge Stigma Sexual orientation Singapore