检索结果-南通市图书馆

Molecular Characterization of RHD in Rh-Negative Blood Donors in Congo Brazzaville

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Biosciences and Medicines 2024年第11期12卷 299-309页

作者： Ludmilhia Gudrid Fouti Landry Fils Mpelle Faust René Okamba Ondzia Love Fresia senda Arsène Bikoue Anicet Luc Magloire Boumba Etienne Nguimbi Faculty of Science and Technology Marien Ngouabi University Brazzaville Republic of the Congo Faculty of Health Sciences Marien Ngouabi University Brazzaville Republic of the Congo National Center of Blood Transfusion Brazzaville Republic of the Congo

Background: The D antigen is the most important and immunogenic antigen of the Rh blood group;its correct screening prevents any risk of alloimmunization in the RHD negative recipient. The D negative phenotype is characterized by the absence of the D antigen (RH1) on the surface of the erythrocyte. Three main mechanisms can generate this absence: total or partial deletion of the RHD gene, insertion of base pairs within the said gene and gene conversion. The objective of this study was to report the first data on RHD genotyping in RHD negative congolese blood donors. Materials and Methods: Blood samples came from regular RHD-negative blood donors selected from the blood transfusion stations in Brazzaville and Pointe-Noire. They were analyzed individually by conventional PCR, targeting exons 4, 5, 7 and 10 of the RHD gene. Results: Fifty-nine regular RHD negative blood donors were selected for this study. The immuno-hematological profile was determined individually, and the dccee phenotype was the most frequent (n = 52;88.1%). The search for the weak D antigen was negative for all donors. Exons 4, 5, 7 and 10 of the RHD gene were amplified in the following respective proportions: 89.8%, 81.4%, 6.8% and 42.4%. Moreover, (1) donor was found to carry all four specific exons sought. Conclusion: Conventional PCR amplification allowed to study the presence of specific exons of the targeted D gene. At least one exon was detected in the entire study population. These results suggest that the RHD gene is indeed present in the donors studied and that the deletion cannot be considered as the main mechanism causing the RH-1 (D negative) phenotype in this sample.

关键词： Molecular Characterization Rhesus RHD Gene Blood Donors

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

樱山之家——关注地球环境的住宅设计

时代建筑 2002年第6期45卷 58-61页

作者：仙田满郭屹民 Mitsuru senda 日本东京工业大学日本建筑学会

本文以“樱山之家”设计为例,着重介绍了在设计过程中对基地周边环境的充分协调和利用,并在此基础上创造丰富的居住空间形态。同时强调了环境设计是关系设计的观点。

关键词：环境设计关系

Hybridization increases genetic diversity in Schistosoma haematobium populations infecting humans in Cameroon

在线全文

学校读者我要写书评

暂无评论

Infectious Diseases of Poverty 2022年第2期11卷 100-100页

作者： Felicite Flore Djuikwo Teukeng Manon Blin Nicolas Bech Marta reguera Gomez Rima Zein-Eddine Alain Michel Kouam Simo Jean-Francois Allienne Louis Albert Tchuem‑Tchuente Jerome Boissier Faculty of Heath Science Mountain UniversityP.O.Box 208BangangteCameroon 不详

Background:Hybrids between Schistosoma haematobium(Sh)and ***(Sb)have been found in several African countries as well as in *** the consequences of this hybridization are still unknown,this study aims to verify the presence of such hybrids in Cameroonian humans,to describe the structure of *** populations on a large geographic scale,and to examine the impact of these hybrids on genetic diversity and structure of these ***:From January to April 2019,urine from infected children was collected in ten geographically distinct popu‑*** were collected from eggs in this *** detect the presence of hybrids among these miracidia we genotyped both Cox1(RD-PCR)and ITS2 gene(PCR-RFLP).Population genetic diversity and structure was assessed by genotyping each miracidium with a panel of 14 microsatellite *** diversity was measured using both heterozygosity and allelic richness indexes,and genetic structure was analyzed using paired Fst,PCA and Bayesian ***:Of the 1327 miracidia studied,88.7% were identifed as pure genotypes of ***(Sh_Sh/Sh)while the remaining 11.3% were hybrids(7.0% with Sh_Sh/Sb,3.7% with Sb_Sb/Sh and 0.4% with Sb_Sh/Sb).No miracidium has been identifed as a pure genotype of *** richness ranged from 5.55(Loum population)to 7.73(MattaBarrage)and difered signifcantly between *** heterozygosity ranged from 53.7%(Loum)to 59%(Matta Barrage)with no signifcant *** overall genetic diferentiation inferred either by a principal compo‑nent analysis or by the Bayesian approach shows a partial *** populations(Loum and Matta Bar‑rage)were clearly separated from other localities but genetic diferentiation between northern localities was limited,certainly due to the geographic proximity between these ***:Hybrids between *** and *** were identifed in 11.3% of miracidia that hatched from eggs present in the urine o

关键词： Schistosoma haematobium Schistosoma bovis Genetic diversity Hybridization Miracidium Cameroon

Potential Technological Use of Reserves of <i>Jatropha curcas</i>and <i>J. macrocarpa</i>Griseb. Seeds

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2019年第8期10卷 1444-1456页

作者： Nancy E. de las M. Tavecchio Marta S. Dardanelli Yanina B. reguera Herminda E. Reinoso Oscar A. Terenti Marisa M. Garbero Hilda E. Pedranzani Departamento de Ciencias Agropecuarias Facultad de Ingeniería y Ciencias Agropecuarias Universidad Nacional de San Luis San Luis Argentina Departamento de Biología Molecular Facultad de Ciencias Exactas Físico-Químicas y Naturales Universidad Nacional de Río Cuarto Córdoba Argentina Departamento de Ciencias Naturales Facultad de Ciencias Exactas Físico-Químicas y Naturales Universidad Nacional de Río Cuarto Córdoba Argentina Laboratorio de semillas EEA INTA San Luis Argentina Departamento de Bioquímica y Ciencias Biológicas Facultad de Química Bioquímica y Farmacia Universidad Nacional de San Luis San Luis Argentina

J. curcas and J. macrocarpa are useful for restoring degraded areas and their seeds contain oils for biodiesel production. The aim of the work was to determine the reserve substances in the endosperm and the embryo of J. curcas and J. macrocarpa which is important in understanding the germination process, the establishment of these species and its industrial employment. Seeds were imbibed in distilled water for 24 h, to facilitate removal of seed coat with the aim to separate the embryo and nutritive tissues. In both species, the endosperm contained aleurone grains consisting of a crystalloid and globoid, lipids of red color and the starch was not observed. Four major fatty acids were determined in J. curcas seed: oleic, palmitic, stearic, palmitoleic and oleic fatty acid represents about 70% oil content. Oleic acid was the most abundant in J. macrocarpa seeds, while, there was not palmitoleic acid. Seed with predominantly unsaturated fatty acids is ideal for biodiesel industry. The means of the sugar content were: 14.3 μg/mg in endosperm and 104.76 μg/mg in embryo of J. curcas and 6.48 μg/mg in endosperm and 59.20 μg/mg in embryo of J. macrocarpa. The means of the protein content were: 4.2 μg/mg in endosperm and 45.02 μg/mg in embryo of J. curcas and 3.26 μg/mg in endosperm and 31.08 μg/mg in embryo of J. macrocarpa. Sugar and protein contents of Jatropha seeds were significantly higher in embryo in both species (p 0.1), which suggests early mobilization towards the embryo during imbibition period.

关键词： Fatty Acids J. curcas J. macrocarpa Proteins Seeds Sugars

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

博物馆·公园——茨城县自然博物馆的设计

建筑学报 2000年第12期 20-24页

作者：仙田满胡惠琴日本建筑学会东京工业大学日本昭和女子大学

1.前言(1)自然教育·环境教育的场所茨城县自然博物馆是1972年开始构思的,1988年决定在横跨岩井市和水海道,占地面积约为16.4公顷的地面上建造自然博物馆.这块用地是由面临240公顷的菅生沼的两个阶梯型的地形和山谷构成的.

关键词：自然博物馆建筑设计公园环境建筑

实时聚合酶链反应:诊断神经型布氏杆菌病的新有效工具

在线全文

学校读者我要写书评

暂无评论

世界核心医学期刊文摘（神经病学分册） 2005年第10期1卷 34-35页

作者： Colmenero J.D. Queipo-Ortuo M.I. reguera J.M. 周永 Unidad de Enfermedades Infecciosas Hospital Universitario Carlos Haya Avda Carlo s Haya s/n 29010 Malaga Spain

Background/methods: We compared the diagnostic yield of a real time polymeras e chain reaction (PCR) assay in cerebrospinal fluid (CSF) samples with conventio nal microbiological techniques for the diagnosis of neurobrucellosis. Following amplification of a 223 bp sequence specific for Brucella genus, melting curve an alysis was performed to verify the specificity of the PCR products. Results: All six patients with neurobrucellosis (three meningitis and three meningoencephali tis) had a positive real time PCR assay, whereas CSF cultures and Wright seroagg lutination tests were positive in only two and four cases, respectively. Brucell a specific amplicons were easily demonstrated by their characteristic melting te mperature in all the real time PCR assays. Conclusion: LightCycler based real ti me PCR assay in CSF samples is more rapid and sensitive than conventional microb iological tests. This technique could be useful for the rapid diagnosis of neuro brucellosis.

关键词：神经型特异序列微生物学检验血清凝集试验脑膜脑炎熔点温度诊断分析特异性扩增产物布氏杆菌属检测诊断

Wood Density Determination with the Perspective to Decarbonisation of Tropical Forest Species from the Luki Biosphere Reserve in the Democratic Republic of the Congo

在线全文

学校读者我要写书评

暂无评论

Green and Sustainable Chemistry 2023年第2期13卷 162-169页

作者： Topwe Milongwe Mwene-Mbeja Luboya Muisangie Jeannette Bukasa Kadima Katanku Kabongo Kanimba Junior Benjamin Kalenda Kabengela N’senda Kamulumba Kayembe Gaby Liyandja Impofi Jean-Claude Lopema Ongala Dénis Mbuyi Mpoyi Alain Center for Research on Water Management and Environment (CREE) Kinshasa Democratic Republic of the Congo Department of Chemistry Faculty of Science University of Lubumbashi Lubumbashi Democratic Republic of the Congo Hydro-Quebec Institute for Environment Development and Society at Laval University Quebec Canada Research Centre for Applied Sciences and Technologies (CRSAT) Kinshasa Democratic Republic of the Congo Faculty of Letters and Human Sciences National Pedagogical University (UPN) Kinshasa Democratic Republic of the Congo

Specimens of the forest species such as Pentaclethra macrophylla, Petersianthus macrocarpus, Pycnanthus angolensis and Terminalia superba have been sampled from LUKI Biosphere reserve in the Democratic Republic of the Congo in order to determine their wood density with the perspective to decarbonisation. These parameters have been found out experimentally utilizing a drying technique in an oven including techniques of immersion in an Erlenmeyer full of water. The corresponding results indicated that the four species wood density is respectively 0.85, 0.80, 0.77 and 0.51. These preliminary results will be useful in our ongoing project on carbon dioxide absorption capacity of Congo rainforest tree species.

关键词： Carbon Dioxide Congo Rainforest Decarbonisation Wood Density

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

顶管技术在深层地下区域中作用的基础研究

非开挖技术 2010年第5期 27-33页

作者： Takashi senda （） Hideki Shimada （） Takashi Sasaoka（） Kikuo Matsui（） Masaya Hirai（） Tomo Morita（）蒲婷（译）

在日本城市地区的地下有许多公用设施，如污水、自来水、天然气、电路等等。在更深层的地下区域安装各种管路和电缆的需求正随着城市化而不断扩大。因此，在深层地下区域中的顶管技术正亟需使用。本文给出了顶管机铺管技术的一些准则和... 详细信息

在日本城市地区的地下有许多公用设施，如污水、自来水、天然气、电路等等。在更深层的地下区域安装各种管路和电缆的需求正随着城市化而不断扩大。因此，在深层地下区域中的顶管技术正亟需使用。本文给出了顶管机铺管技术的一些准则和一些行业数据。同时，文章还分析了在粘土层和砂质土壤中顶管机所受的推进力和摩擦阻力特性。文章还讨论了在土壤中和深层地区的沉积岩中。

关键词：顶管深部力学特性

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

游戏环境的设计方法及其发展

世界建筑 2016年第11期 27-32,119页

作者：仙田满辛梦瑶环境设计研究所儿童环境学会

儿童的游戏环境具有游戏空间、游戏时间、游戏方法和游戏集体4个要素。游戏空间应与其他3个要素联合起来考虑。同时,游戏空间应按照自然空间、开放空间、道路空间、无秩序空间、秘密集会空间、游具空间等6种基本空间类型来考虑。本文提... 详细信息

儿童的游戏环境具有游戏空间、游戏时间、游戏方法和游戏集体4个要素。游戏空间应与其他3个要素联合起来考虑。同时,游戏空间应按照自然空间、开放空间、道路空间、无秩序空间、秘密集会空间、游具空间等6种基本空间类型来考虑。本文提出一种能激发儿童游戏的空间构造,包含7个条件,称为游环构造。许多儿童设施都基于该构造的原理被设计,其有效性获得了证实。

关键词：游戏环境游戏空间游具幼儿园儿童中心