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Physical chemical and biological characterization of a new bacteriocin produced by Bacillus cereus NS02

Asian Pacific Journal of Tropical Medicine 2013年第12期6卷 934-941页

作者： senbagam d Gurusamy R Senthilkumar B department of Biotechnology Vivekanandha College of Engineering for Women Tiruchengode-637 205Tamil NaduIndia department of Biotechnology Muthayammal College of Arts and ScienceRasipuram-637 408.Tamil NaduIndia

Objective:To screen the bacteriocinogenic isolate from buffalo milk and to characterize it on physical,chemical and biological aspects for the application in ***:Bacillus cereus(***)was isolated and assessed for its baceteriocinogenic *** was produced and purified by ammonium sulphate precipitation,dialysis and gel filtration *** bacteriocin was used to clieck its antimicrobial activity against food borne *** and stability of bacteripcin was determined with the respect to temperature,pH,enzymes,organic solvents and *** was also subjected to SdS PAGE analysis to determine its molecular *** addition,functional groups exist in the bacteriocin was determined by FTIR ***:*** was identified by 16S rRNA sequence *** showed increased activity against all the bacteria used and its activity unit was found to be 51,200 AU/*** was stable to high temperature(100℃)and wide range of pH(3-10),sensitive to proteolytic enzymes and resistant to nonprotcolytic *** was low molecular weight(3.5-6 kda)protein and FTIR study revealed the presence of amide group and NH stretching,Conclusions:Bacteriocin produced in this study possesses the highest antimicrobial activity against both gram positive and gram negative bacteria thereby it has immense application as biopreservative *** proved its peptide nature.

关键词： Bacillus cereus Bacteriocin Antimicrobial activity FTIR

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Induction of deletion mutation on ompR gene of Salmonella enterica serovar Typhi isolates from asymptomatic typhoid carriers to evolve attenuated strains for vaccine development

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Asian Pacific Journal of Tropical Medicine 2014年第12期7卷 933-939页

作者： Senthilkumar B Anbarasu K senbagam d Rajasekarapandian M Centre for Biotechnology Muthayammal College of Arts & SeienceRasipuram-637408Tamil NaduIndia department of Marine Biotechnology Bharathidasan UniversityTiruchirappalli-620024.Tamil NaduIndia department of Biotechnology Virekanandha College of Engineering for WomenTiruchengode-637205.Tamil NaduIndia department of Zoology Arignar Anna Government Arts CollegeNamakkal-637001Tamil NaduIndia

Objective:To develop allenualed slrains of Salmonella enterica serorar Typhi(***) for the candidate vaccine by osmolar ***:*** SS3 and SS5 strains were isolated from asymptomatic typhoid carriers in Mamakkal,Tamil *** strains were grown in LB(Luria Bertani) medium supplemented with various concentration of NaCl(0.1- 0.7M) *** effecl of osmolar stress was determined at molecular level by PCR using MCR 06 and MCR07 primers corresponding to ompR with chromosomal dNA of *** SS3 and SS5 *** by osmolar stress results in deletion mutation of *** slrains was determined by agglutination assays,precipitation *** PAGE analysis and by animal ***:The 799 bp amplified ompR gene product from wild type *** SS3 and SS5 illustrate the presence of virulent ***,there was only a 282 bp amplified product from *** SS3 and SS5 grown in the presence of 0.***.0.6 and 0.7 M *** illustrates the occurrence of deletion mutation in ompR gene al high concentration of ***,both the wildtype and mutant *** outer membrane *** reveals the differences in the expression of *** and ompA *** mice,wild type and mutant strains lethal dose(Ld_(50)) were *** mice died within 72 h when both the wild type strains were injected intraperitoneally with 3 log CFU-mL^(-1).When the mice were injected with the mutants in same dosage,no clinical symptoms were observed;whereas the serum antibodv litre was elicited within two weeks indicated that the mutants have the ability to induce protective humoral immune *** results suggest that *** SS3 and SS5 may bo used as good candidate strains for the development of live attenuated vaccine against ***:This study demonstrates that the *** strains were allenualed and could be good vaccine candidates in future.

关键词： Attenuated live vaccine Salmonella enterica serovar Typhi Mutants

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