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TET1 Participates in Complete Freund’s Adjuvant-induced Trigeminal Inflammatory Pain by Regulating Kv7.2 in a Mouse Model

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Neuroscience Bulletin 2024年第6期40卷 707-718页

作者： Dengcheng zhan Jingjing zhang Songxue Su Xiuhua Ren sen Zhao Weidong Zang Jing Cao Department of Human Anatomy School of Basic Medical SciencesZhengzhou UniversityZhengzhou450001China Neuroscience Research Institute Zhengzhou UniversityZhengzhou450001China

Trigeminal inflammatory pain is one of the most severe pain-related disorders in humans;however,the underlying mechanisms remain largely *** this study,we investigated the possible contribution of interaction between ten-eleven translocation methylcytosine dioxygenase 1(TET1)and the voltage-gated K^(+)channel Kv7.2(encoded by Kcnq2)to orofacial inflammatory pain in *** found that complete Freund’s adjuvant(CFA)injection reduced the expression of Kcnq2/Kv7.2 in the trigeminal ganglion(TG)and induced orofacial inflammatory *** involvement of Kv7.2 in CFA-induced orofacial pain was further confirmed by Kv7.2 knockdown or ***,TET1 knockdown in Tet1^(flox/flox)mice significantly reduced the expression of Kv7.2 and M currents in the TG and led to pain-like ***,TET1 overexpression by lentivirus rescued the CFA-induced decreases of Kcnq2 and M currents and alleviated mechanical *** data suggest that TET1 is implicated in CFA-induced trigeminal inflammatory pain by positively regulating Kv7.2 in TG neurons.

关键词： Facial pain KCNQ2 potassium channel TET1 protein

Intrinsically large effective mass and multi-valley band characteristics of n-type Bi_(2)eBi_(2)Te_(3)superlattice-like films

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Journal of Materiomics 2024年第3期10卷 716-724页

作者： Yujie Ouyang Min zhang Fangyang zhan Chunxia Li Xianda Li Fan Yan sen Xie Qiwei Tong Haoran Ge Yong Liu Rui Wang Wei Liu Xinfeng Tang State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan430070China International School of Materials Science and Engineering Wuhan University of TechnologyWuhan430070China Institute for Structure and Function&Department of Physics Chongqing UniversityChongqing400044China School of Physics and Technology and the Key Laboratory of Artificial Micro/Nano Structures of Ministry of Education Wuhan UniversityWuhan430072China

Thermoelectric superlattices are expected to decouple the strong correlation between various thermo-electric parameters,and are an important strategy for excellent thermoelectric *** superlattices of(Bi_(2))m(Bi_(2)Te_(3))n homologous series are well-known for low lattice thermal conductivity and intriguing topological surface ***,the impacts of electronic structure on the thermo-electric performance were still not well-understood in(Bi_(2))m(Bi_(2)Te_(3))*** cope with this issue,Bi_(2)eBi_(2)Te_(3)superlattice-like films with adjustable Bi_(2)/(Bi_(2)+Bi_(2)Te_(3))molar ratio(R)were successfully fabricated by the molecular beam epitaxy ***-resolved photoemission spectroscopy measurements com-bined with theoretical calculations revealed the conduction band evolution from single-valley to multi-valley as R≥0.30,leading to intrinsically high carrier effective mass and improved thermoelectric power ***,the superlattice film(R=0.46)with the structure close to Bi_(4)Te_(3)possesses the topological surface state feature around the high symmetry *** a result of the high effective mass of 3.9 m0 and very high electron density of_(2).31×10^(21)cm^(-3),the film with R=0.46 acquired the highest power factor of 1.49 mW·m^(-1)·K^(-2)at 420 K,outperforming that of other(Bi_(2))m(Bi_(2)Te_(3))n *** work lays an essential foundation on understanding the electronic structure and further improving thermoelectric performances of(Bi_(2))m(Bi_(2)Te_(3))n homologous series.

关键词： Bi_(2)eBi_(2)Te_(3)superlattice Molecular beam epitaxy Band structure Thermoelectric performance

Achieving single-cell-resolution lineage tracing in zebrafish by continuous barcoding mutations during embryogenesis

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Journal of Genetics and Genomics 2024年第9期51卷 947-956页

作者： zhan Liu Hui Zeng Huimin Xiang Shanjun Deng Xionglei He State Key Laboratory of Biocontrol School of Life SciencesSun Yat-Sen UniversityGuangzhouGuangdong 510275China

Unraveling the lineage relationships of all descendants from a zygote is fundamental to advancing our understanding of developmental and stem cell ***,existing cell barcoding technologies in zebrafish lack the resolution to capture the majority of cell divisions during embryogenesis.A recently developed method,a substitution mutation-aided lineage-tracing system(SMALT),successfully reconstructed high-resolution cell phylogenetic trees for Drosophila ***,we implement the SMALT system in zebrafish,recording a median of 14 substitution mutations on a one-kilobase-pair barcoding sequence for one-day post-fertilization *** this system,we reconstruct four cell lineage trees for zebrafish fin cells,encompassing both original and regenerated *** tree consists of hundreds of internal nodes with a median bootstrap support of 99%.Analysis of the obtained cell lineage trees reveals that regenerated fin cells mainly originate from cells in the same part of the *** multiple times sampling germ cells from the same individual,we show the stability of the germ cell pool and the early separation of germ cell and somatic cell *** system offers the potential for reconstructing high-quality cell phylogenies across diverse tissues,providing valuable insights into development and disease in zebrafish.

关键词： Lineage tracing Cellular barcoding Zebrafishcell phylogeny Fin regeneration Germcell lineage

A study of the effect of local scour on the flow field near the spur dike

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Theoretical & Applied Mechanics Letters 2024年第2期14卷 98-105页

作者： Yu-Tian Li Jie-Min zhan Wing-Hong Onyx Wai Department of Industrial Design School of Industrial Design and Ceramic ArtFoshan UniversityFoshan 528000China Department of Applied Mechanics and Engineering Shenzhen Campus of Sun Yat-sen UniversityShenzhen 518107China Department of Civil and Environmental Engineering The Hong Kong Polytechnic UniversityHong Kong 999077China

The flow field near a spur dike such as down flow and horseshoe vortex system(HVS)are susceptible to the topographic changes in the local scouring process,resulting in variation of the sediment transport with *** this study,large eddy simulations with fixed-bed at different scouring stages were conducted to investigate the changes in flow *** results imply that the bed deformation leads to an increase in flow rate per unit area,which represent the capability of sediment transportation by water,in the scour ***,the intensity of turbulent kinetic energy and bimodal motion near the sand bed induced by the HVS were also ***,the peak moments between the two sediment transport mechanisms were ***,understanding the complex feedback mechanism between topography and flow field is essential for the local scour problem.

关键词： Spur dike Local scour Large eddy simulation Down flow Horseshoe vortex system

基于UPLC指纹图谱和多成分测定的天王补心丸质量控制研究

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中国中药杂志 2024年第5期49卷 1240-1248页

作者：沈丹萍姜鹏詹常森上海和黄药业有限公司上海201401 上海中药固体制剂创新工程技术研究中心上海201401

天王补心丸临床上已有一定疗效,但严重缺少较全面的质量控制,以期保证临床用药安全性和有效性。研究采用超高效液相色谱(UPLC)建立天王补心丸指纹图谱和7个成分含量同时测定的方法,并结合化学计量学等方法寻找天王补心丸质量稳定的关键... 详细信息

天王补心丸临床上已有一定疗效,但严重缺少较全面的质量控制,以期保证临床用药安全性和有效性。研究采用超高效液相色谱(UPLC)建立天王补心丸指纹图谱和7个成分含量同时测定的方法,并结合化学计量学等方法寻找天王补心丸质量稳定的关键因素,为其科学全面的质量控制和评价提供参考。建立了15批天王补心丸指纹图谱,相似度均大于0.96,共标定了25个共有峰,指认出13个成分,对其中7个药效成分(α-亚麻酸、丹酚酸B、甘草酸、五味子醇甲、β-细辛醚、3,6′-二芥子酰基蔗糖和藁本内酯)建立含量测定方法,进一步采用主成分分析(principal component analysis,PCA)和偏最小二乘法-判别分析(partial least square discriminate analysis,PLS-DA)得到控制天王补心丸质量稳定性的关键药效物质为3,6′-二芥子酰基蔗糖、α-亚麻酸和β-细辛醚。结果表明建立的指纹图谱及多成分含量测定方法专属性强,稳定,可靠,提示3,6′-二芥子酰基蔗糖、α-亚麻酸和β-细辛醚是保障天王补心丸批次间质量稳定性的关键药效物质,可较为全面地对天王补心丸进行质量控制,为其质量评价和标准建立提供科学依据。

关键词：天王补心丸指纹图谱多成分含量测定统计学质量评价

TREM-2 Drives Development of Multiple Sclerosis by Promoting Pathogenic Th17 Polarization

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Neuroscience Bulletin 2024年第1期40卷 17-34页

作者： Siying Qu Shengfeng Hu Huiting Xu Yongjian Wu Siqi Ming Xiaoxia zhan Cheng Wang Xi Huang Center for Infection and Immunity and Guangdong Provincial Engineering Research Center of Molecular Imaging The Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhai 519000China The State Key Laboratory of Respiratory Disease Guangdong Provincial Key Laboratory of Allergy and Clinical ImmunologyThe Second Affiliated HospitalGuangzhou Medical UniversityGuangzhou 510260China Department of Laboratory Medicine The First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080China Division of Nephrology Department of Medicinethe Fifth Affliated Hospital of Sun Yat-sen UniversityZhuhai 519000China

Multiple sclerosis(MS)is a neuroinflammatory demyelinating disease,mediated by pathogenic T helper 17(Th17)***,the therapeutic effect is accompa-nied by the fluctuation of the proportion and function of Th17 cells,which prompted us to find the key regulator of Th17 differentiation in ***,we demonstrated that the triggering receptor expressed on myeloid cells 2(TREM-2),a modulator of pattern recognition receptors on innate immune cells,was highly expressed on pathogenic CD4-positive T lymphocyte(CD4^(+)T)cells in both patients with MS and experimental autoimmune encephalomyelitis(EAE)mouse *** knockout of Trem-2 in CD4^(+)T cells significantly alleviated the disease activity and reduced Th17 cell infiltration,activation,differentiation,and inflammatory cytokine production and secretion in EAE ***,with Trem-2 knockout in vivo experiments and in vitro inhibitor assays,the TREM-2/zeta-chain associated protein kinase 70(ZAP70)/signal transducer and activator of transcription 3(STAT3)signal axis was essential for Th17 activation and differentiation in EAE *** conclusion,TREM-2 is a key regulator of pathogenic Th17 in EAE mice,and this sheds new light on the potential of this therapeutic target for MS.

关键词： Multiple sclerosis Experimental autoimmune encephalomyelitis T helper 17 Triggering receptor expressed on myeloid cells 2

Magnetic field stabilization system designed for the cold-atom coherent population-trapping clock

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Chinese Optics Letters 2024年第8期22卷 7-12页

作者： Chang zhan Zhu Ma Jiatao Wu Maojie Li Chengyin Han Bo Lu Chaohong Lee Laboratory of Quantum Engineering and Quantum Metrology School of Physics and AstronomySun Yat-sen UniversityZhuhai 519082China Institute of Quantum Precision Measurement State Key Laboratory of Radio Frequency Heterogeneous IntegrationShenzhen UniversityShenzhen 518060China College of Physics and Optoelectronic Engineering Shenzhen UniversityShenzhen 518060China Quantum Science Center of Guangdong-Hong Kong-Macao Greater Bay Area Shenzhen 518045China

Accurate control of magnetic fields is crucial for cold-atom experiments,often necessitating custom-designed control systems due to limitations in commercially available power ***,we demonstrate precise and flexible control of a static magnetic field by employing a field-programmable gate array and a feedback *** setup enables us to maintain exceptionally stable current with a fractional stability of 1 ppm within 30 *** error signal of the feedback loop exhibited a noise level of 10~(-5)A·Hz~(-1/2)for control bandwidths below 10 k *** this precise magnetic field control system,we investigate the second-order Zeeman shift in the context of cold-atom coherent population-trapping (CPT)*** analysis reveals the second-order Zeeman coefficient to be 574.21 Hz/G~2,with an uncertainty of 1.36 Hz/G~***,the magnetic field stabilization system we developed allows us to achieve a second-order Zeeman shift below10~(-14),surpassing the long-term stability of current cold-atom CPT clocks.

关键词： cold-atom coherent population-trapping clock Zeeman coefficient magnetic field stabilization system

Unveiling the role of hypoxia-inducible factor 2alpha in osteoporosis:Implications for bone health

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World Journal of Stem Cells 2024年第4期16卷 389-409页

作者： Ling-Ling Wang zhan-Jin Lu Shun-Kui Luo Yun Li Zhe Yang Hong-Yun Lu Department of Gerontology The Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhai 519000Guangdong ProvinceChina Department of Endocrinology and Metabolism The Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhai 519000Guangdong ProvinceChina Guangdong Provincial Key Laboratory of Biomedical Imaging The Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhai 519000Guangdong ProvinceChina Department of Endocrinology and Metabolism Zhuhai People’s Hospital(Zhuhai Clinical Medical College of Jinan Universitythe First Hospital Affiliated with Medical College of Macao University of Science and Technology)Zhuhai 519000Guangdong ProvinceChina

BACKGROUND Osteoporosis(OP)has become a major public health problem *** OP treatments are based on the inhibition of bone resorption,and it is necessary to identify additional treatments aimed at enhancing *** the bone marrow(BM)niche,bone mesenchymal stem cells(BMSCs)are exposed to a hypoxic ***,a few studies have demonstrated that hypoxiainducible factor 2alpha(HIF-2α)is involved in BMSC osteogenic differentiation,but the molecular mechanism involved has not been *** To investigate the effect of HIF-2αon the osteogenic and adipogenic differentiation of BMSCs and the hematopoietic function of hematopoietic stem cells(HSCs)in the BM niche on the progression of *** Mice with BMSC-specific HIF-2αknockout(Prx1-Cre;Hif-2αfl/fl mice)were used for in vivo *** quantification was performed on mice of two genotypes with three interventions:Bilateral ovariectomy,semilethal irradiation,and dexamethasone ***,the hematopoietic function of HSCs in the BM niche was compared between the two mouse *** vitro,the HIF-2αagonist roxadustat and the HIF-2αinhibitor PT2399 were used to investigate the function of HIF-2αin BMSC osteogenic and adipogenic ***,we investigated the effect of HIF-2αon BMSCs via treatment with the mechanistic target of rapamycin(mTOR)agonist MHY1485 and the mTOR inhibitor *** The quantitative index determined by microcomputed tomography indicated that the femoral bone density of Prx1-Cre;Hif-2αfl/fl mice was lower than that of Hif-2αfl/fl mice under the three intervention *** vitro,Hif-2αfl/fl mouse BMSCs were cultured and treated with the HIF-2αagonist roxadustat,and after 7 d of BMSC adipogenic differentiation,the oil red O staining intensity and mRNA expression levels of adipogenesis-related genes in BMSCs treated with roxadustat were decreased;in addition,after 14 d of osteogenic differentiation,BMSCs treated with roxadusta

关键词： Hypoxia-inducible factor-2α Bone marrow niche Bone mesenchymal stem cells Osteoporosis Osteogenic/adipogenic differentiation Mechanistic target of rapamycin signaling pathway

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变压器套管介损异常案例分析

电工技术 2024年第17期 129-132页

作者：占鹏杨森广州供电局广东广州510410

对2起110 kV变压器套管介损异常案例进行分析。采用油色谱、绝缘电阻、10 kV常规介损、高电压介损与FDS试验结合,分析套管可能存在的缺陷,并对其中1起套管案例进行解体检查,找出缺陷的原因。所开展的案例分析可为发现变压器套管缺陷隐... 详细信息

对2起110 kV变压器套管介损异常案例进行分析。采用油色谱、绝缘电阻、10 kV常规介损、高电压介损与FDS试验结合,分析套管可能存在的缺陷,并对其中1起套管案例进行解体检查,找出缺陷的原因。所开展的案例分析可为发现变压器套管缺陷隐患提供思路与参考。

关键词：变压器套管 FDS试验高电压介损