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Interaction with general transcription factor ⅡF (TFⅡF) is required for the suppression of activated transcription by RPB5-mediating protein(RMP)

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Cell Research 2003年第2期 111-120页

作者： DORJBAL DORJSUREN,seishi MURAKAMI Department of Molecular Oncology Cancer Research Institute Kanazawa University Takara-machi 13-1 Kanazawa 920-0934 Japan Department of Molecular Oncology Cancer Research Institute Kanazawa University Takara-machi 13-1 Kanazawa 920-0934 Japan

RMP was reported to regulate transcription via competing with HBx to bind the general transcription factor IIB (TFIIB) and interacting with RPB5 subunit of RNA polymerase Ⅱ as a corepressor of transcription regulator. However, our present research uncovered that RMP also regulates the transcription through interaction with the general transcription factors IIF (TFIIF), which assemble in the preinitiation complex and function in both transcription initiation and elongation. With in vitro pull-down assay and Far-Western analysis, we demonstrated that RMP could bind with bacterially expressed recombinant RAP30 and RAP74 of TFIIF subunits. In the immunoprecipitation assay in COS1 cells cotransfected with FLAG-tagged RMP or its mutants, GST-fused RAP30 and RAP74 were co-immunoprecipitated with RMP in approximately equal molar ratio, which suggests that RAP30 and RAP74 interact with RMP as a TFIIF complex. Interestingly both RAP30 and RAP74 interact with the same domain (D5) of the C-terminal RMP of 118-amin

关键词： general transcription factor IIF RPB5-mediating protein activated transcription RNA polymerase Ⅱ subunit 5.

Plant Phenomics:Emerging Transdisciplinary Science

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Plant Phenomics 2019年第1期1卷 39-41页

作者： seishi ninomiy. Frédéric Baret Zong-Ming(Max)Cheng University of Tokyo TokyoJapan French National Institute of Agricultural Research ParisFrance Nanjing Agricultural University NanjingChina University of Tennessee TNUSA

Humankind is facing an unprecedented challenge to produce enough food for the coming decades because of population growth and increase in the average demand per capita,changes in climate conditions,and limitations in arable land area,as well as pressure on the water and *** main avenues should be concurrently taken to increase crop productivity:improving genetics to get more efficient and resilient crops and developing optimal crop management *** description and understanding of crop functioning will therefore be instrumental both for genetic improvement and crop *** will help to associate functional traits with the genome which will accelerate genetic progress by having more efficient techniques to design ideotypes adapted to particular pedoclimatic and crop management conditions and create them from the available genetic diversity.

关键词： crops Plant adapted

Analyzing Nitrogen Effects on Rice Panicle Development by Panicle Detection and Time-Series Tracking

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Plant Phenomics 2023年第2期5卷 253-267页

作者： Qinyang Zhou Wei Guo Na Chen Ze Wang Ganghua Li Yanfeng Ding seishi ninomiy. Yue Mu College of Agriculture Academy for Advanced Interdisciplinary StudiesCollaborative Innovation Center for Modern Crop Production co-sponsored by Province and MinistryNanjing Agricultural UniversityNanjing 210095China Graduate School of Agricultural and Life Sciences The University of Tokyo1-1-1 Midori-choNishi-TokyoTokyo 188-0002Japan

Detailed observation of the phenotypic changes in rice panicle substantially helps us to understand the yield *** recent studies,phenotyping of rice panicles during the heading–flowering stage still lacks comprehensive analysis,especially of panicle development under different nitrogen *** this work,we proposed a pipeline to automatically acquire the detailed panicle traits based on time-series images by using the YOLO v5,ResNet50,and DeepSORT *** with field observation data,the proposed method was used to test whether it has an ability to identify subtle differences in panicle developments under different nitrogen *** result shows that panicle counting throughout the heading–flowering stage achieved high accuracy(R^(2)=0.96 and RMSE=1.73),and heading date was estimated with an absolute error of 0.25 *** addition,by identical panicle tracking based on the time-series images,we analyzed detailed flowering phenotypic changes of a single panicle,such as flowering duration and individual panicle flowering *** rice population,with an increase in the nitrogen application:panicle number increased,heading date changed little,but the duration was slightly extended;cumulative flowering panicle number increased,rice flowering initiation date arrived earlier while the ending date was later;thus,the flowering duration became *** a single panicle,identical panicle tracking revealed that higher nitrogen application led to earlier flowering initiation date,significantly longer flowering days,and significantly longer total duration from vigorous flowering beginning to the end(total DBE).However,the vigorous flowering beginning time showed no significant differences and there was a slight decrease in daily DBE.

关键词： heading identical flowering

Hepatitis B virus X protein induces hepatic stem cell-like features in hepatocellular carcinoma by activating KDM5B

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World Journal of Gastroenterology 2017年第18期23卷 3252-3261页

作者： Xuyang Wang Naoki Oishi Tetsuro Shimakami Taro Yamashita Masao Honda seishi Murakami Shuichi Kaneko Department of Disease Control and Homeostasis Kanazawa University Graduate School of Medical Science Department of Gastroenterology Kanazawa University Hospital

To determine the role of hepatitis B virus X protein (HBx), HBx in regulating hepatic progenitor cell (HPC)-like features in hepatocellular carcinoma (HCC) and the underlying molecular *** used a retrovirus vector to introduce wild type HBx or empty vector into HepG2 cells. We then used these cells to analyze cell proliferation, senescence, transformation, and stem-like features. Gene expression profiling was carried out on Affymetrix GeneChip Human U133A2.0 ver.2 arrays according to the manufacturer’s protocol. Unsupervised hierarchical clustering analysis and Class Comparison analysis were performed by BRB-Array Tools software Version 4.2.2. A total of 238 hepatitis B virus (HBV)-related HCC patients’ array data were used for analyzing clinical *** histone demethylase KDM5B was significantly highly expressed in HBV-related HCC cases (P < 0.01). In HBV proteins, only HBx up-regulated KDM5B by activating c-myc. Hepatic stem cell (HpSC) markers (EpCAM, AFP, PROM1, and NANOG) were significantly highly expressed in KDM5B-high HCC cases (P < 0.01). KDM5B played an important role in maintaining HpSC-like features and was associated with a poor prognosis. Moreover, inhibition of KDM5B suppressed spheroid formation and cell invasion in *** activates the histone demethylase KDM5B and induces HPC-like features in HCC. Histone demethylases KDM5B may be an important therapeutic target against HBV-related HCC cases.

关键词： Hepatitis B virus X protein Hepatocellular carcinoma KDM5B Progenitor cell Tumorigenesis

Buckling Load Evaluation Method for Single Layer Cylindrical Lattice Shells

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Journal of Civil Engineering and Architecture 2012年第3期6卷 268-279页

作者： seishi Yamada Department of Architecture and Civil Engineering Toyohashi University of Technology Toyohashi 441-8580 Japan

A rational design evaluation procedure is investigated for the elastic overall buckling load carrying capacity of single layer cylindrical lattice shell roof structures. The nature of the imperfection sensitivity of these structures is for the first time reviewed in this paper. This allows the development of the reduced stiffness buckling analytical concept for the lattice shells based upon the introduction of a simple lower bound estimation equation through the use of the so-called continuum shell analogy theory. The linear and nonlinear buckling loads found from conventional finite element analyses are compared with the present estimations. Finally, the elastic-plastic load carrying capacity estimation method through the use of the present elastic lower bound criteria is also proposed.

关键词： Metal lattice shell Buckling design formula cylindrical barrel vault roof structure.

Can the biliary enhancement of Gd-EOB-DTPA predict the degree of liver function?

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Hepatobiliary & Pancreatic Diseases International 2012年第3期11卷 307-313页

作者： Masahiro Okada Kazunari Ishii Kazushi Numata Tomoko Hyodo seishi Kumano Masayuki Kitano Masatoshi Kudo Takamichi Murakami Department of Radiology Kinki University Faculty of Medicine Gastroenterological Center Yokohama City University Medical Center Department of Radiology and Department of Gastroenterology and Hepatology Kinki University Faculty of Medicine

BACKGROUND: Excretion of gadolinium-ethoxybenzyl-diethyl- enetriamine pentaacetic acid (Gd-EOB-DTPA) in the bile may be related to liver function, because of elimination from the liver after preferential uptake by hepatocytes. The purpose of this study was to investigate the relation between liver and biliary enhancement in patients with or without liver dysfunction, and to compare the tumor-to-liver contrast in these patients. METHODS: Forty patients [group 1: normal liver and Child Pugh class A in 20 patients, group 2: Child-Pugh class B in 18 patients and Child-Pugh C in 2] were evaluated. All patients underwent MR imaging of the liver using a 1.5-Tesla system. T1 weighted 3D images were obtained at 5, 10, 15 and 20 minutes after Gd-EOB-DTPA injection. The relation between group 3 (total bilirubin <1.8 mg/dL) and group 4 (total bilirubin ≥1.8 mg/dL) was investigated at 20 minutes. Liver and biliary signals were measured, and compared between groups 1 and 2 or groups 3 and 4. Tumor-to-liver ratio was also evaluated between groups 1 and 2. Scheffé’s post-hoc test after two-way repeated measures ANOVA and Pearson’s correlation test were used for statistical analysis. RESULTS: Liver enhancement showed significant difference at all time points between groups 1 and 2. Biliary enhancement did not show a significant difference between groups 1 and 2 at 5 minutes, but did at 10, 15 and 20 minutes. At 20 minutes significant differences between groups 3 and 4 were seen for liver and biliary enhancement. At all time points, liver enhancement correlated with biliary enhancement in both groups. At 5 minutes and 20 minutes, statistical differences between groups 1 and 2 were seen for tumor-to-liver ***: The degree of biliary enhancement has a close correlation to that of liver enhancement. It is especially important that insufficient liver enhancement causes lower tumor-to-liver contrast in the hepatobiliary phase of Gd-EOB- DTPA.

关键词： magnetic resonance imaging Gd-EOB-DTPA liver bile duct hepatobiliary phase

The Japanese agricultural case-base InformationRetrieval System using Semi-Discrete MatrixDecomposition

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The Japanese agricultural case-base InformationRetrieval Sys...

Effective Utilization of Concrete Sludge as Soil Improvement Materials

国际农业工程大会

作者： seishi ninomiy. HeadNetwork Computing Lab. Dept. Information Science ＆ Technology National Agriculture Research Center 3-1-1 Kannondai Tsukuba City Ibaraki 305-8666 Japan

＜正＞ In this paper, we present a Japanese document retrieval system that uses a new method of matrix *** order to improve the precision and recall ratio in the document retrieval system, a mathematical method called...

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Journal of Civil Engineering and Architecture 2014年第6期8卷 783-789页

作者： seishi Tomohisa Yasuyuki Nabeshima Toshiki Noguchi Yuya Miura Department of Civil Engineering Akashi National College of Technology Hyogo 6748501 Japan Department of Architecture and Civil Engineering Akashi National College of Technology Hyogo 6748501 Japan

The amount of muddy soil generated from various kinds of construction sites is always problematic. It is very difficult to treat muddy soil because of its low strength and high water content. But, the reuse of muddy soil is necessary to reduce the total amount of industrial wastes. Surplus concrete is also in a similar situation. Coarse and fine aggregates are removed from surplus concrete as an intermediate treatment, however, concrete sludge still remains. The authors propose a reuse method that involves the muddy soil being mixed with concrete sludge as an improvement material. The possibility of the utilization of concrete sludge was investigated through laboratory experiments. As a result, it was found that the unconfined compressive strength of the improved soil mixed with concrete sludge increased as the curing proceeded.

关键词： Reuse concrete sludge muddy soil improvement material curing process.

STATIC SEISMIC LOAD MODELING USING CONTINUUM SHELL ANALOGY FOR SINGLE LAYER LATTICED CYLINDRICAL SHELL STRUCTURES

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STATIC SEISMIC LOAD MODELING USING CONTINUUM SHELL ANALOGY F...