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Proteome alterations in aqueous humour of primary open angle glaucoma patients

International Journal of Ophthalmology(English edition) 2020年第1期13卷 176-179页

作者： Hanhan Liu Fabian Anders sebastian funke Karl Mercieca Franz Grus Verena Prokosch Department of Ophthalmology University Medical Center of the Johannes Gutenberg University MainzMainz 55131Germany Experimental and Translational Ophthalmology Department of OphthalmologyUniversity Medical Center of the Johannes Gutenberg University MainzMainz 55131Germany Royal Eye Hospital School of MedicineUniversity of ManchesterManchester M202ULUnited Kingdom

AIM: To unravel the primary open angle glaucoma(POAG) related proteomic changes in aqueous humour(AH).METHODS: Totally 35 patients listed for cataract surgery(controls: n=12, age: 67.4±13.6 y) or trabeculectomy for POAG(n=23, age: 72.5±8.3 y) were included. AH samples of those patients were obtained during cataract surgery or trabeculectomy. AH samples were subsequently pooled into the experimental groups under equal contribution in terms of protein amount of each individual patient. Protein samples were analyzed by a linear trap quadrupol Orbitrap Mass Spectrometry device with an upstream liquid chromatography system. The obtained raw data were analyzed using the Maxquant proteome software and compared. Proteins with a fold-change ratio higher than a cut-off of 2 were considered as noticeably ***: A total number of 175 proteins could be identified out of the AH from POAG and cataract by means of quantitative mass spectrometric analysis. Apolipoprotein D(fold change, 3.16 times), complement C3(2.96), pigment epithelium-derived factor(2.86), dickkopf-related protein 3(2.18) and wingless-related integration(Wnt) inhibitory factor 1(2.35) were significantly upregulated within the AH of glaucoma compared to cataract serving as ***: AH provides a tool to analyze changes in glaucoma and shows striking changes in Wnt signaling inhibitory molecules and other proteins.

关键词： primary open angle glaucoma aqueous humor proteomics Wnt signaling pathway

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