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The Role of Phosphorylation in Redox Regulation of Photosynthesis Genes psaA and psbA during Photosynthetic Acclimation of Mustard

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Molecular Plant 2009年第3期2卷 416-429页

作者： sebastian steiner Lars Dietzel Yvonne Schroter Vidal Fey Raik Wagner Thomas Pfannschmidt Junior Research Group 'Plant acclimation to environmental changes: Protein analysis by MS' at the Institute of General Botany and Plant Physiology Department of Plant Physiology Friedrich-Schiller-University of Jena Dornburger Str. 159 07743 Jena Germany Present address: VTT Technical research Center of Finland Medical Biotechnology Turku Finland Present address: Department of Chemistry Bioenergetics University of Umea Sweden

The long-term response （LTR） to light-quality gradients improves performance and survival of plants in dense stands. It involves redox-controlled transcriptional regulation of the plastome-encoded genes psaAB （encoding the P700 apoproteins of photosystem I） and psbA （encoding the D1 protein of photosystem II） and requires the action of plastidlocalized kinases. To study the potential impact of phosphorylation events on plastid gene expression during the LTR, we analyzed mustard seedlings acclimated to light sources favoring either photosystem I or photosystem II. Primer extension analyses of psaA transcripts indicate that the redox regulation occurs at the principal bacterial promoters, suggesting that the plastid encoded RNA polymerase （PEP） is the target for redox signals. Chloroplast protein fractions containing PEP and other DNA-binding proteins were purified from mustard via heparin-Sepharose chromatography. The biochemical properties of these fractions were analyzed with special emphasis on promoter recognition and specificity, phosphorylation state, and kinase activity. The results demonstrate that the LTR involves the action of small DNA-binding proteins; three of them exhibit specific changes in the phosphorylation state. Auto-phosphorylation assays, in addition, exhibit large differences in the activity of endogenous kinase activities. Chloroplast run-on transcription experiments with the kinase inhibitor H7 and the reductant DTT indicate that phosphorylation events are essential for the mediation of redox signals toward psaA and psbA transcription initiation, but require the synergistic action of a thiol redox signal. The data support the idea that redox signals from the thylakoid membrane are linked to gene expression via phosphorylation events; however, this mediation appears to require a complex network of interacting proteins rather than a simple phosphorelay.

关键词： Light-quality acclimation redox control protein phosphorylation chloroplast transcription Sinapis alba.

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Modulus spectroscopy for the detection of parallel electric responses in electroceramics

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Journal of Materiomics 2022年第3期8卷 556-569页

作者： Till Fromling Yao Liu An-Phuc Hoang Maximillian Gehringer sebastian steiner Mikalai Zhuk Julia Glaum Bai-Xiang Xu Department of Materials and Earth Science Technical University of DarmstadtFG Nichtmetallisch-Anorganische WerkstoffeAlarich-Weiss-Straße 264287DarmstadtGermany Department of Materials and Earth Science Technical University of DarmstadtFG Mechanics of Functional MaterialsOtto-Berndt-Straße 364287DarmstadtGermany Department of Materials Science and Engineering Norwegian University of Science and Technology(NTNU)Functional Materials and Materials Chemistry Group(FACET)Sem Sælands Vei 127491TrondheimNorway

Impedance spectroscopy has become one of the most versatile and essential investigation methods concerning electrical properties of materials for electronic and energy *** knowledge about physical mechanisms,however,often demands excellent expertise in evaluating the *** different representations of the same data set can help elucidate the underlying physics,but this is rarely *** this work,the importance of using the modulus representation to identify parallel electric responses is *** responses result from parallel conducting pathways,e.g.,at grain boundaries,or from regions with differing permittivity,e.g.,in *** and quantitative data can be obtained,as it is illustrated based on experimental data from electroceramics and respective physical simulation results using the finite element *** findings should help to study intricate electric responses of materials with chemical or structural heterogeneity.

关键词： ceramics spectroscopy versatile

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